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E. coli Homologous Recombination: How It Works and Influences on Cloning
Homologous recombination is the exchange of sequences that occurs between two similar (homologous)...
Understanding Auxotrophic Mutants: Yeast and Bacterial Cloning Tools
Auxotrophic mutants are bacteria, yeast, protoplast or mammalian host cell strains that can’t produc...
All About the Turbidity-Based Lysozyme Activity Assay
Lysozyme is an enzyme that breaks down peptidoglycan, and it is often used in protein purification,...
What are E. coli Expression Strains?
E. coli expression strains, BL21(DE3) and DL39 for example, are used for recombinant RNA/protein exp...
Explain Alpha-Complementation and Blue-White Screening To Me Like I’m 10
Alpha-complementation – it’s a big phrase, but it’s not terribly hard to understand. The technical...
E. coli Cloning Strains for Molecular Biology: What They Are and Why They Matter
loning strains are very specific bacterial strains used in molecular biology to clone recombinant DN...
All About Differential Media in Gene Cloning - What it is and How it Works
Differential media is culture media containing compounds that enable visual distinction between the...
Selective Media Used in Gene Cloning
Selective media helps researchers identify their desired mutant host cell colonies during gene cloni...
Basic and Advanced Fluorescence Microscopy Techniques
Fluorescence microscopy spans from basic epifluorescence/widefield microscopy to advanced techniques...