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Shop By Category | Buffers

Buffers
Buffers are essential in many different biological and biotechnical applications. GoldBio’s vast array of application-specific buffers include biological, electrophoretic and lysis buffers in order to ensure that we have exactly the right product for both your research needs as well as your budget.
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5X Phosphate Buffer + 3M Imidazole Solution
Catalog ID
Size
Pricing
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5X Phosphate Buffer + 3M Imidazole Solution product image
I-905-50
50 mL
$ 117.00
I-905-100
100 mL
$ 168.00
Description
This Buffer Set consists of: - 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl) - 25 mL 3M Recrystallized Imidazole Solution, pH 6.0This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification. View product page →
Specifications
CAS Number
Technical Resources
5X Phosphate Buffer, pH 8
Catalog ID
Size
Pricing
More Info
5X Phosphate Buffer, pH 8 product image
P-500-50
50 mL
$ 54.00
P-500-100
100 mL
$ 76.00
P-500-250
250 mL
$ 108.00
Description
5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag. View product page →
Specifications
CAS Number
Technical Resources
ACES
Catalog ID
Size
Pricing
More Info
ACES product image
A-010-25
25 g
$ 25.00
A-010-50
50 g
$ 39.00
A-010-100
100 g
$ 50.00
Description

ACES is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is an acetamido buffer that is useful for a pH range of 6.1 – 7.5. ACES is used as buffering component in cell culture media, in both agarose and polyacrylamide electrophoresis, and in isoelectric focusing of proteins. ACES forms a complex with most common metals and formation constants should be taken into account when using this buffer in a solution containing metal ions. ACES absorbs UV light at 230 nm.

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Specifications
CAS Number 7365-82-4
Technical Resources
ADA
Catalog ID
Size
Pricing
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ADA product image
A-780-25
25 g
$ 19.00
A-780-50
50 g
$ 24.00
A-780-100
100 g
$ 35.00
Description
ADA is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is an acetamido buffer that is useful for a pH range of 6.0 – 7.2. ADA forms a complex with most common metals and stability constants should be taken into account when using this buffer. It has been used as a complexing agent to effectively help remove contaminant metals from soil. ADA absorbs UV light between 0.1 and 260 nm. Solubility of ADA increases if the free acid is converted to the sodium salt with NaOH. Product Specifications ADA; MHA; N-(2-acetamido)iminodiacetic View product page →
Specifications
CAS Number 26239-55-4
Technical Resources
Bacterial Cell Lysis Buffer
Catalog ID
Size
Pricing
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Bacterial Cell Lysis Buffer product image
GB-177-100
100 mL
$ 54.00
GB-177-500
500 mL
$ 106.00
GB-176-100
100 Preps
$ 152.00
GB-176-250
250 Preps
$ 268.00
GB-176-500
500 Preps
$ 495.00
Description

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Bacterial Lysis Buffer is based on organic buffering agents and utilizes a mild nonionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into the Bacterial Lysis Buffer. It has been tested for use with several widely used bacterial strains and is recommended for E. coli and gram-negative bacteria.

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Specifications
CAS Number
Technical Resources
BES
Catalog ID
Size
Pricing
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BES product image
B-780-25
25 g
$ 19.00
B-780-50
50 g
$ 29.00
B-780-100
100 g
$ 40.00
Description
BES is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is a bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 6.4 – 7.8. It is soluble in water at 0°C up to 3.2M. Like most neutral, amine-based buffers, BES forms a complex with DNA. It interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in BES shields the View product page →
Specifications
CAS Number 10191-18-1
Technical Resources
Bicine
Catalog ID
Size
Pricing
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Bicine product image
B-785-25
25 g
$ 17.00
B-785-50
50 g
$ 22.00
B-785-100
100 g
$ 30.00
B-785-500
500 g
$ 120.00
B-785-1
1 kg
$ 199.00
Description
Bicine is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 7.6 – 9.0. It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, as well as a multiphasic buffer system for SDS-PAGE of proteins. It is soluble in water at 0°C up to 1.1 M. Bicine forms a complex with most common metals so stability constants and concentrations View product page →
Specifications
CAS Number 150-25-4
Technical Resources
Bis-Tris
Catalog ID
Size
Pricing
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Bis-Tris product image
B-020-100
100 g
$ 51.00
B-020-250
250 g
$ 78.00
B-020-500
500 g
$ 119.00
B-020-1
1 kg
$ 205.00
Description

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

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Specifications
CAS Number 6976-37-0
Technical Resources
Boric Acid, ACS Grade
Catalog ID
Size
Pricing
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Boric Acid, ACS Grade product image
B-030-500
500 g
$ 15.00
B-030-1
1 kg
$ 25.00
B-030-5
5 kg
$ 110.00
Description

Boric acid is commonly used for the preparation of buffers such as Tris-Borate-EDTA (TBE). Borate buffers have been used in capillary and gel electrophoresis for separating proteins and nucleic acids. The useful buffering range of boric acid is pH 8.2-10.1. In a basic solution, the salt of boric acid forms complex equilibria, with the tetraborate ion (B4O72-) being the most predominant species present. Boric acid is used in catalytic amounts to promote amidation between carboxylic acids and amines. It is capable of forming a complex with various carbohydrates, as well as with pyridine nucleotides, such as NAD+.

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Specifications
CAS Number 10043-35-3
Technical Resources
Boric Acid, Biotechnology Grade
Catalog ID
Size
Pricing
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Boric Acid, Biotechnology Grade product image
B-031-500
500 g
$ 18.00
B-031-1
1 kg
$ 27.00
B-031-5
5 kg
$ 120.00
Description

Boric acid is commonly used for the preparation of buffers such as Tris-Borate-EDTA (TBE). Borate buffers have been used in capillary and gel electrophoresis for separating proteins and nucleic acids. The useful buffering range of boric acid is pH 8.2-10.1. In a basic solution, the salt of boric acid forms complex equilibria, with the tetraborate ion (B4O72-) being the most predominant species present. Boric acid is used in catalytic amounts to promote amidation between carboxylic acids and amines. It is capable of forming a complex with various carbohydrates, as well as with pyridine nucleotides, such as NAD+.

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Specifications
CAS Number 10043-35-3
Technical Resources
CAPS
Catalog ID
Size
Pricing
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CAPS product image
C-040-25
25 g
$ 19.00
C-040-50
50 g
$ 29.00
C-040-100
100 g
$ 40.00
C-040-500
500 g
$ 99.00
Description

CAPS is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a cyclohexylamine buffer that is useful for a pH range of 9.7 – 11.1 and commonly used to study enzymatic processes above physiological pH. CAPS is an excellent buffer for the transfer of high molecular weight proteins to nitrocellulose membranes after SDS-PAGE. It is also used in protein sequencing. CAPS displays only weak or negligible complexation capabilities for some metals making it a suitable non-coordinating buffer for use in solutions containing metal ions. CAPS is suitable for use with the bicinchoninic acid (BCA) assay.

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Specifications
CAS Number 1135-40-6
Technical Resources
CHES
Catalog ID
Size
Pricing
More Info
CHES product image
C-870-25
25 g
$ 20.00
C-870-50
50 g
$ 24.00
C-870-100
100 g
$ 35.00
C-870-500
500 g
$ 130.00
Description
CHES is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a cyclohexylamine buffer that is useful for a pH range of 8.6 – 10.0 and commonly used to study enzymatic processes above physiological pH. CHES displays only weak or negligible complexation capabilities for some metals making it a suitable non-coordinating buffer for use in most solutions containing metal ions. CHES displays high affinity for the iodoacetate binding site of liver alcohol dehydrogenase, protecting the enzyme from inactivation by iodoacetic acid. Product Specifications CHES; 2-(Cyclohexylamino)ethanesulfonic acid; N-Cyclohexyltaurine; MOLECULAR BIOLOGY GRADE Formula: C8H17NO3S MW: 207.29 g/mol PubChem Chemical ID: 66898 Storage/Handling: Store at room temperature. View product page →
Specifications
CAS Number 103-47-9
Technical Resources
HEPES, Free Acid
Catalog ID
Size
Pricing
More Info
HEPES, Free Acid product image
H-400-100
100 g
$ 32.00
H-400-250
250 g
$ 64.00
H-400-500
500 g
$ 97.00
H-400-1
1 kg
$ 151.00
H-400-2
2 kg
$ 252.00
H-400-5
5 kg
$ 535.00
Description

HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

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Specifications
CAS Number 7365-45-9
Technical Resources
HEPES, Sodium Salt
Catalog ID
Size
Pricing
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HEPES, Sodium Salt product image
H-401-50
50 g
$ 31.00
H-401-100
100 g
$ 52.00
H-401-500
500 g
$ 154.00
H-401-1
1 kg
$ 258.00
H-401-2.5
2.5 kg
$ 571.00
H-401-5
5 kg
$ 993.00
Description
HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent View product page →
Specifications
CAS Number 75277-39-3
Technical Resources
HEPPSO
Catalog ID
Size
Pricing
More Info
HEPPSO product image
H-480-25
25 g
$ 39.00
H-480-50
50 g
$ 54.00
H-480-100
100 g
$ 75.00
Description
HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 7.1 – 8.5. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations View product page →
Specifications
CAS Number 68399-78-0
Technical Resources
His-Tag Buffer Set
Catalog ID
Size
Pricing
More Info
His-Tag Buffer Set product image
I-906-250
1 kit
$ 108.00
Description
His-Tag Buffer Set includes: - 250 mL Wash Buffer (50mM Phosphate Buffer pH 8, 300 mM NaCl, 10mM Imidazole) - 50 mL Elution Buffer (50mM Phosphate Buffer, 300 mM NaCl, 500mM Imidazole)Designed for use with Goldbio Nickel and Cobalt Agarose resins, but it can be used with most standard His-tag purification resins. View product page →
Specifications
CAS Number
Technical Resources
Imidazole - 3M Recrystallized Solution, pH 6.0
Catalog ID
Size
Pricing
More Info
Imidazole - 3M Recrystallized Solution, pH 6.0 product image
I-901-25
25 mL
$ 64.00
I-901-50
50 mL
$ 95.00
I-901-100
100 mL
$ 147.00
I-901-250
250 mL
$ 340.00
Description
Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.Recrystallized Imidazole from GoldBio is specially purified Imidazole specifically designed for Histidine tagged protein purification. We purify the Imidazole at Gold Biotechnology’s labs and go through multiple purification steps including ultrafiltration and recrystallization to obtain the most purified Imidazole on the market. The final Imidazole View product page →
Specifications
CAS Number 288-32-4
Technical Resources
Imidazole, Recrystallized
Catalog ID
Size
Pricing
More Info
Imidazole, Recrystallized  product image
I-902-25
25 g
$ 64.00
I-902-50
50 g
$ 108.00
I-902-100
100 g
$ 173.00
I-902-500
500 g
$ 530.00
Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

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Specifications
CAS Number 288-32-4
Technical Resources
Mammalian Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Mammalian Cell Lysis Buffer product image
GB-180-100
100 mL
$ 54.00
GB-180-500
500 mL
$ 110.00
Description

The Mammalian Cell Lysis Buffer is ideal for extracting total, biologically active, soluble proteins from mammalian cells. Rather than using mechanical means for cell lysis, the mammalian cell lysis buffer simplifies the process, utilizing a mild, nonionic detergent, chelating agent and a proprietary combination of various salts and agents to improve the extraction and stability of proteins. It is compatible with electrophoresis and chromatographic applications, as well as being compatible with various kinases, protease inhibitors and immunoassays such as ELISA and RIA.

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Specifications
CAS Number
Technical Resources
MES Free Acid Monohydrate, Ultra Pure
Catalog ID
Size
Pricing
More Info
MES Free Acid Monohydrate, Ultra Pure product image
M-090-100
100 g
$ 51.00
M-090-250
250 g
$ 78.00
M-090-500
500 g
$ 114.00
M-090-1
1 kg
$ 195.00
Description

MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

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Specifications
CAS Number 145224-94-8
Technical Resources
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