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Shop By Category | Cloning & Induction

Cloning & InductionGold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.
Displaying 1-8 of 8 results.
5-Fluoroorotic acid monohydrate (5-FOA)
Catalog ID
Size
Pricing
More Info
5-Fluoroorotic acid monohydrate (5-FOA) product image
F-230-2.5
2.5 g
$ 54.00
F-230-5
5 g
$ 103.00
F-230-10
10 g
$ 173.00
F-230-25
25 g
$ 318.00
Description

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

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Specifications
CAS Number 220141-70-8
Technical Resources
Bacterial Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Bacterial Cell Lysis Buffer product image
GB-177-100
100 mL
$ 54.00
GB-177-500
500 mL
$ 106.00
GB-176-100
100 Preps
$ 152.00
GB-176-250
250 Preps
$ 268.00
GB-176-500
500 Preps
$ 495.00
Description

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Bacterial Lysis Buffer is based on organic buffering agents and utilizes a mild nonionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into the Bacterial Lysis Buffer. It has been tested for use with several widely used bacterial strains and is recommended for E. coli and gram-negative bacteria.

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Specifications
CAS Number
Technical Resources
IPTG
Catalog ID
Size
Pricing
More Info
IPTG product image
I2481C
1 g
$ 16.00
I2481C5
5 g
$ 25.00
I2481C25
25 g
$ 108.00
I2481C50
50 g
$ 173.00
I2481C100
100 g
$ 329.00
I2481C200
200 g
$ 636.00
I2481C300
300 g
$ 848.00
I2481C400
400 g
$ 1101.00
I2481C500
500 g
$ 1327.00
Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

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Specifications
CAS Number 367-93-1
Technical Resources
IPTG EZ Pak™ for 1 M Solution
Catalog ID
Size
Pricing
More Info
IPTG EZ Pak™ for 1 M Solution product image
I2481-EZ10
10 mL
$ 54.00
I2481-EZ50
50 mL
$ 86.00
I2481-EZ100
100 mL
$ 141.00
Description

The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

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Specifications
CAS Number 367-93-1
Technical Resources
Mammalian Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Mammalian Cell Lysis Buffer product image
GB-180-100
100 mL
$ 54.00
GB-180-500
500 mL
$ 110.00
Description

The Mammalian Cell Lysis Buffer is ideal for extracting total, biologically active, soluble proteins from mammalian cells. Rather than using mechanical means for cell lysis, the mammalian cell lysis buffer simplifies the process, utilizing a mild, nonionic detergent, chelating agent and a proprietary combination of various salts and agents to improve the extraction and stability of proteins. It is compatible with electrophoresis and chromatographic applications, as well as being compatible with various kinases, protease inhibitors and immunoassays such as ELISA and RIA.

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Specifications
CAS Number
Technical Resources
Tissue Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Tissue Cell Lysis Buffer product image
GB-181-100
100 mL
$ 54.00
GB-181-500
500 mL
$ 110.00
Description
Tissue Cell Lysis Buffer has been developed for extraction of total biologically active, soluble proteins from animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer and utilizes a mild nonionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into Tissue Buffer. Tissue Cell Lysis Buffer has been tested for use with a wide variety of fresh and frozen animal tissues. View product page →
Specifications
CAS Number
Technical Resources
Yeast Lysis Buffer
Catalog ID
Size
Pricing
More Info
Yeast Lysis Buffer product image
GB-179-100
100 mL
$ 54.00
GB-179-500
500 mL
$ 110.00
Description
The Yeast Cell Lysis kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast Lysis Buffer is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast Lysis Buffer is based on organic buffering agents and utilizes a mild nonionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works well with both gram-positive View product page →
Specifications
CAS Number
Technical Resources
Yeast Lysis Buffer plus Zymolyase
Catalog ID
Size
Pricing
More Info
Yeast Lysis Buffer plus Zymolyase product image
GB-178
100 Preps
$ 158.00
Description
The Yeast Cell Lysis Kit has been developed for extraction of biologically active, soluble proteins from yeast cells. Yeast Lysis Buffer is a proprietary improvement on the lyticase (Zymolyase®) based spheroplast preparation and extraction of soluble proteins from yeast cells. Yeast Lysis Buffer is based on organic buffering agents and utilizes a mild nonionic detergent, chelating agent, and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided. Depending on the required downstream application, additional agents such as reducing agents and protease inhibitors may be added into the Yeast View product page →
Specifications
CAS Number
Technical Resources