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Shop By Category | Cloning & Induction

Gold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.
Displaying 1-20 of 35 results.
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5-Fluoroorotic acid monohydrate (5-FOA)
Catalog ID
Size
Pricing
More Info
5-Fluoroorotic acid monohydrate (5-FOA) product image
F-230-2.5
2.5 g
$ 54.00
F-230-5
5 g
$ 103.00
F-230-10
10 g
$ 173.00
F-230-25
25 g
$ 318.00
Description

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

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Specifications
CAS Number 220141-70-8
Technical Resources
Bacterial Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Bacterial Cell Lysis Buffer product image
GB-177-100
100 mL
$ 54.00
GB-177-500
500 mL
$ 106.00
GB-176-100
100 Preps
$ 160.00
GB-176-250
250 Preps
$ 281.00
GB-176-500
500 Preps
$ 518.00
Description
The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.Bacterial Cell Lysis Buffer is based on organic buffering agents and utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of View product page →
Specifications
CAS Number
Technical Resources
Cas9 Nuclease (10µM)
Catalog ID
Size
Pricing
More Info
C-529-50
50 µl
$ 150.00
C-529-250
250 µl
$ 495.00
Description
Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie blue staining.CRISPR (Clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system View product page →
Specifications
CAS Number
Technical Resources
Cas9 Nuclease (1µM)
Catalog ID
Size
Pricing
More Info
C-519-250
250 µl
$ 90.00
Description
Cas9 Nuclease is the purified recombinant Streptococcus pyogenes Cas9 enzyme containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR/Cas9-mediated genome editing. The physical purity of this enzyme is ≥98% as assessed by SDS-PAGE with Coomassie blue staining.CRISPR (Clustered regularly interspaced short palindromic repeats)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. The CRISPR system consists of a short non-coding guide RNA (sgRNA) made View product page →
Specifications
CAS Number
Technical Resources
Hot Start Pfu DNA Polymerase
Catalog ID
Size
Pricing
More Info
P-650-40
40 μl
$ 130.00
P-650-200
200 μl
$ 590.00
Description
Hot Start Pfu DNA Polymerase is formulated with chemical modification which effectively neutralize 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities at room temperature, but regain the full enzyme activity upon the initial denaturation step. Hot start Pfu DNA polymerase retains the high fidelity, sensitivity and processivity of Pfu DNA polymerase, while provides reduced background by facilitating room temperature PCR assembly and preventing priming until stringent primer annealing temperatures are reached. Pfu DNA polymerase has an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the View product page →
Specifications
CAS Number
Technical Resources
Hot Start Pfu DNA Polymerase plus dNTP
Catalog ID
Size
Pricing
More Info
P-655-40
40 μl
$ 144.00
P-655-200
200 μl
$ 640.00
Description
Hot Start Pfu DNA Polymerase is formulated with chemical modification which effectively neutralize 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities at room temperature, but regain the full enzyme activity upon the initial denaturation step. Hot start Pfu DNA polymerase retains the high fidelity, sensitivity and processivity of Pfu DNA polymerase, while provides reduced background by facilitating room temperature PCR assembly and preventing priming until stringent primer annealing temperatures are reached. Pfu DNA polymerase has an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the View product page →
Specifications
CAS Number
Technical Resources
Hot Start Taq 2x Master Mix - 20 μl reaction
Catalog ID
Size
Pricing
More Info
T-513-100
100 reactions
$ 40.00
T-513-500
500 reactions
$ 130.00
Description
Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer that has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start View product page →
Specifications
CAS Number
Technical Resources
Hot Start Taq 2x Master Mix - 50 μl reaction
Catalog ID
Size
Pricing
More Info
T-512-100
100 reactions
$ 70.00
T-512-500
500 reactions
$ 210.00
Description
Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer that has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification View product page →
Specifications
CAS Number
Technical Resources
Hot Start Taq DNA Polymerase
Catalog ID
Size
Pricing
More Info
T-510-40
40 μl
$ 60.00
T-510-200
200 μl
$ 250.00
Description
Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´ flap endonuclease activity. Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of View product page →
Specifications
CAS Number
Technical Resources
Hot Start Taq DNA Polymerase plus dNTP
Catalog ID
Size
Pricing
More Info
T-511-40
40 μl
$ 74.00
T-511-200
200 μl
$ 295.00
Description
Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity and a 5´ flap endonuclease activity. Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC View product page →
Specifications
CAS Number
Technical Resources
IPTG
Catalog ID
Size
Pricing
More Info
IPTG product image
I2481C
1 g
$ 16.00
I2481C5
5 g
$ 25.00
I2481C25
25 g
$ 108.00
I2481C50
50 g
$ 173.00
I2481C100
100 g
$ 329.00
I2481C200
200 g
$ 636.00
I2481C300
300 g
$ 848.00
I2481C400
400 g
$ 1101.00
I2481C500
500 g
$ 1327.00
Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

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Specifications
CAS Number 367-93-1
Technical Resources
IPTG EZ Pak™ for 1 M Solution
Catalog ID
Size
Pricing
More Info
IPTG EZ Pak™ for 1 M Solution product image
I2481-EZ10
10 mL
$ 54.00
I2481-EZ50
50 mL
$ 86.00
I2481-EZ100
100 mL
$ 141.00
Description

The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

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Specifications
CAS Number 367-93-1
Technical Resources
Mammalian Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Mammalian Cell Lysis Buffer product image
GB-180-100
100 mL
$ 54.00
GB-180-500
500 mL
$ 110.00
Pfu 2x DNA Polymerase Master Mix
Catalog ID
Size
Pricing
More Info
P-660-50
50 reactions
$ 75.00
P-660-200
200 reactions
$ 240.00
Description
Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.The 2x Premix with Dye comes ready to use, containing Pfu DNA Polymerase, dNTPs, MgCl2 and View product page →
Specifications
CAS Number
Technical Resources
Pfu DNA Polymerase
Catalog ID
Size
Pricing
More Info
P-665-40
40 μl
$ 115.00
P-665-200
200 μl
$ 490.00
Description
Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%. Product Includes View product page →
Specifications
CAS Number
Technical Resources
Pfu DNA Polymerase plus dNTP
Catalog ID
Size
Pricing
More Info
P-690-40
40 μl
$ 129.00
P-690-200
200 μl
$ 540.00
Description
Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with a 10x PCR reaction buffer that provides a final concentration of 1.5mM Mg 2+ and an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%. Product Includes View product page →
Specifications
CAS Number
Technical Resources
T4 DNA Helicase (gp41)
Catalog ID
Size
Pricing
More Info
T-417-1
1 ml
$ 350.00
T-417-5
5 ml
$ 1025.00
Description
T4 DNA helicase (gp41) is a hexameric, DnaB-like helicase that unwinds DNA with 5′ to 3′ polarity. Helicase activity has been shown to be stimulated upon association with gp61 or in coupled assays with the T4 DNA polymerase gp43. T4 replication helicase (gp41) and polymerase (gp43) can carry out ATP-dependent strand displacement DNA synthesis at physiological rates with high processivity in the absence of other T4 DNA replication proteins. T4 DNA helicase is free from detectable nuclease activities. Product Includes T4 DNA helicase (gp41 protein)10X gp41 Reaction BufferPurity: ≥98% (assessed by SDS-PAGE with Coomassie View product page →
Specifications
CAS Number
Technical Resources

No resources are available for this product.

T4 DNA Ligase - 2000 units/μl
Catalog ID
Size
Pricing
More Info
T-411-50
50 μl
$ 165.00
T-411-200
200 μl
$ 299.00
Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzymes joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Product Includes T4 DNA Ligase10x T4 DNA Ligase Reaction BufferPurity: ≥99% (assessed by SDS-PAGE with Coomassie blue staining)Concentration: 2000 unit/μlOne unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (250 ng/µl) in View product page →
Specifications
CAS Number
Technical Resources
T4 DNA Ligase - 400 units/μl
Catalog ID
Size
Pricing
More Info
T-410-50
50 μl
$ 60.00
T-410-250
250 μl
$ 165.00
Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5’-phosphate and 3’-hydroxyl termini in duplex DNA or RNA. This enzymes joins DNA fragments with either cohesive or blunt termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids. Product Includes T4 DNA Ligase10x T4 DNA Ligase Reaction BufferPurity: ≥99% (assessed by SDS-PAGE with Coomassie blue staining)Concentration: 400 unit/μlOne unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (250 ng/µl) in a total reaction volume View product page →
Specifications
CAS Number
Technical Resources
T4 DNA Polymerase
Catalog ID
Size
Pricing
More Info
T-412-25
25 μl
$ 50.00
T-412-100
100 μl
$ 150.00
Description
T4 DNA Polymerase has both a DNA-dependent DNA polymerase activity and a potent 3´→5´ exonuclease activity. T4 DNA Polymerase is idea for removal of 3´-overhangs or filling in 5´-overhang to form blunt ends, ligation-independent cloning of PCR products, or second strand synthesis in site-directed mutagenesis. Our T4 polymerase is proven to be free from detectable endonuclease and RNase activities.  Product Includes T4 DNA Polymerase10x T4 DNA Polymerase Reaction BufferPurity: ≥95% (assessed by SDS-PAGE with Coomassie blue staining)Concentration: 5 unit/μlOne unit of T4 DNA Polymerase converts 10 nmol of dNTPs into acid-insoluble material in 30 minutes at 37°C under standard View product page →
Specifications
CAS Number
Technical Resources
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