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Shop By Category | Cloning & Induction

Cloning & InductionGold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.
Displaying 1-8 of 8 results.
5-Fluoroorotic acid monohydrate (5-FOA)
Catalog ID
Size
Pricing
More Info
5-Fluoroorotic acid monohydrate (5-FOA) product image
F-230-2.5
2.5 g
$ 54.00
F-230-5
5 g
$ 103.00
F-230-10
10 g
$ 173.00
F-230-25
25 g
$ 318.00
Description

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

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Specifications
CAS Number 220141-70-8
Technical Resources
Bacterial Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Bacterial Cell Lysis Buffer product image
GB-177-100
100 mL
$ 54.00
GB-177-500
500 mL
$ 106.00
GB-176-100
100 Preps
$ 152.00
GB-176-250
250 Preps
$ 268.00
GB-176-500
500 Preps
$ 495.00
Description
The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.Bacterial Cell Lysis Buffer is based on organic buffering agents and utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of View product page →
Specifications
CAS Number
Technical Resources
IPTG
Catalog ID
Size
Pricing
More Info
IPTG product image
I2481C
1 g
$ 16.00
I2481C5
5 g
$ 25.00
I2481C25
25 g
$ 108.00
I2481C50
50 g
$ 173.00
I2481C100
100 g
$ 329.00
I2481C200
200 g
$ 636.00
I2481C300
300 g
$ 848.00
I2481C400
400 g
$ 1101.00
I2481C500
500 g
$ 1327.00
Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

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Specifications
CAS Number 367-93-1
Technical Resources
IPTG EZ Pak™ for 1 M Solution
Catalog ID
Size
Pricing
More Info
IPTG EZ Pak™ for 1 M Solution product image
I2481-EZ10
10 mL
$ 54.00
I2481-EZ50
50 mL
$ 86.00
I2481-EZ100
100 mL
$ 141.00
Description

The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

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Specifications
CAS Number 367-93-1
Technical Resources
Mammalian Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Mammalian Cell Lysis Buffer product image
GB-180-100
100 mL
$ 54.00
GB-180-500
500 mL
$ 110.00
Tissue Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Tissue Cell Lysis Buffer product image
GB-181-100
100 mL
$ 54.00
GB-181-500
500 mL
$ 110.00
Description
Tissue Cell Lysis Buffer has been developed for extraction of total soluble protein from fresh or frozen animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue Cell Lysis Buffer. Tissue Cell Lysis Buffer reagent has been tested for use with a wide variety of animal tissues.. View product page →
Specifications
CAS Number
Technical Resources
Yeast Lysis Buffer
Catalog ID
Size
Pricing
More Info
Yeast Lysis Buffer product image
GB-179-100
100 mL
$ 54.00
GB-179-500
500 mL
$ 110.00
Description
The Yeast Lysis Buffer is useful for extraction of soluble proteins from yeast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer. The Yeast Lysis View product page →
Specifications
CAS Number
Technical Resources
Yeast Lysis Buffer plus Zymolyase
Catalog ID
Size
Pricing
More Info
Yeast Lysis Buffer plus Zymolyase product image
GB-178
100 Preps
$ 158.00
Description
The Yeast Lysis Buffer is useful for extraction of soluble proteins from yeast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.This kit is provided with an optional protocol to make spheroplast and remove lytic enzyme Zymolyase®, prior to lysis and extraction of yeast proteins. Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided. Depending on application, additional agents such View product page →
Specifications
CAS Number
Technical Resources