Toll Free: 800.248.7609
Connect with Us
1-O-Methyl-β-D-glucuronic acid, sodium salt is an inducer of β-glucuronidase, a glycosidase enzyme coded by the GUS gene.
The GUS gene is the most extensively used reporter gene, particularly in plant biology because it offers the advantage of having no background activity.
β-glucuronidase also has an important role within human digestion because this enzyme is capable of deconjugating toxins within the body and releasing them back freely into the bloodstream. There is a positive relationship between higher levels of β-glucuronidase in the colon and increased cancer risk. However, β-glucuronidase, at certain levels, is necessary, and deficiencies can result in mucopolysaccharidosis type VII or Sly syndrome in newborns.View product page →
No resources are available for this product.
Tetrazolium red is used to visualize dehydrogenase enzyme activity. Initially, the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions, which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.View product page →
2-Naphthyl caprylate is a chromogenic substrate for carboxylesterase and lipase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a diazonium salt, the corresponding azo-dye is formed.
Naphthols can also be detected by fluorescence analysis, and can be used in a colorimetric assay for the extracellular lipase of Pseudomonas fluorescent B52.View product page →
P-aminophenyl phosphate (pAPP) can be used as a substrate for alkaline phosphatase enzymatic reactions in order to electrochemically quantify ALP activity.
The enzymatic reaction results in the production of phenol, which can be quantified and used to analyze ALP concentrations.
ALP is a naturally occurring enzyme and can be found in human tissue in the liver, bone and intestines. Usual concentrations of ALP range between 25-100 IU/L. A significant spike outside of that range could act as a signal for many diseases.View product page →
4-Methylumbelliferyl palmitate is used as a fluorogenic substrate for a subclass of esterases called lipase.
Lipase is an enzyme that assists in the hydrolysis of fats. They have a large role in digestion as well as dietary lipid transport.
Lysosomal acid lipase (LAL), in particular, is required for the hydrolysis of cholesterol esters which are then delivered into the lysosome. Deficiencies in LAL are primarily associated with two diseases: cholesteryl ester storage disease (CESD) and Wolman’s disease (WD). 4-Methylumbelliferyl palmitate has often been used as one of the lipase substrates in studies involving either of these diseases.View product page →