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Shop By Category | Protein Expression & Purification

Protein Expression & Purification
Proteins are fundamental macromolecules found in every biological system and form the basis of many fields of work from research into drug creation to industrial applications like fermentation. GoldBio protein products are ideal for every step of your protein synthesis. Our reagents and tools are of the highest quality available at a fraction of the prices of other vendors, so you can be assured that your research yields confident results without compromising your budget.
Displaying 1-20 of 108 results.
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1 kb DNA Ladder
Catalog ID
Size
Pricing
More Info
1 kb DNA Ladder product image
D010-500
1x500 μl
$ 44.00
D010-1000
2x500 μl
$ 82.00
D010-1500
3x500 μl
$ 115.00
D010-2000
4x500 μl
$ 152.00
D010-2500
5x500 μl
$ 174.00
D010-3000
6x500 μl
$ 190.00
Description

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

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Specifications
CAS Number
Technical Resources
1 kb PLUS™ DNA Ladder
Catalog ID
Size
Pricing
More Info
1 kb PLUS™ DNA Ladder product image
D011-500
1x500 μl
$ 55.00
D011-1000
2x500 μl
$ 103.00
D011-1500
3x500 μl
$ 147.00
D011-2000
4x500 μl
$ 185.00
D011-2500
5x500 μl
$ 217.00
D011-3000
6x500 μl
$ 239.00
Description

The 1 kb PLUS™ is composed of 14 individual DNA fragments: 25K, 10K, 8K, 6K, 5K, 4K, 3K, 2.5K, 2K, 1500, 1000, 750, 500, 250 base pairs. This product contains two enhanced bands (3K and 1K bp) for easy reference. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

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Specifications
CAS Number
Technical Resources
1,2-Dipalmitoyl-rac-glycero-3-phosphocholine
Catalog ID
Size
Pricing
More Info
1,2-Dipalmitoyl-rac-glycero-3-phosphocholine product image
D-226-500
0.5 g
$ 155.00
D-226-1
1 g
$ 290.00
D-226-2.5
2.5 g
$ 677.00
D-226-5
5 g
$ 1265.00
Description
1,2-Dipalmitoyl-rac-glycero-3-phosphocholine is a phospholipid with two palmitic acids and is the racemic mixture of Dipalmitoylphosphatidylcholine (also known as Colfosceril palmitate or DPPC). The phosphocholine head is sensitive to electric charges at the membrane surface when bound to pure phospholipids. When added to a solution containing charged ions, this sensitivity can shift the nitrogen cation away from a lack of positive charge or towards a positive charge in solution. In a research environment, it is useful in studying changes of biological membranes and liposomes. Because of its ability to form simple phospholipid bilayers, it is useful in computer modeling of bilayers View product page →
Specifications
CAS Number 2644-64-6
Technical Resources
100 bp DNA Ladder
Catalog ID
Size
Pricing
More Info
100 bp DNA Ladder product image
D001-500
1x500 μl
$ 44.00
D001-1000
2x500 μl
$ 82.00
D001-1500
3x500 μl
$ 115.00
D001-2000
4x500 μl
$ 152.00
D001-2500
5x500 μl
$ 174.00
D001-3000
6x500 μl
$ 190.00
Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

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Specifications
CAS Number
Technical Resources
100 bp PLUS™ DNA Ladder
Catalog ID
Size
Pricing
More Info
100 bp PLUS™ DNA Ladder product image
D003-500
1x500 μl
$ 44.00
D003-1000
2x500 μl
$ 82.00
D003-1500
3x500 μl
$ 120.00
D003-2000
4x500 μl
$ 152.00
D003-2500
5x500 μl
$ 174.00
D003-3000
6x500 μl
$ 190.00
Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

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Specifications
CAS Number
Technical Resources
4-Nitrophenyl phosphate, disodium salt hexahydrate
Catalog ID
Size
Pricing
More Info
4-Nitrophenyl phosphate, disodium salt hexahydrate product image
N-380-50
50 g
$ 81.00
N-380-100
100 g
$ 126.00
N-380-250
250 g
$ 279.00
N-380-500
500 g
$ 475.00
Description
4-Nitrophenyl phosphate, disodium salt hexahydrate PNPP, p-nitrophenyl phosphate, para nitrophenyl phosphate, PNP-phos · 2 Na MOLECULAR BIOLOGY GRADE C6H4NO6PNa2·6H2O MW: 371.15 g/mol Off-white to light yellow crystalline powder Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases. Used for determining alkaline phosphatase activity in human serum. Storage/Handling: Store at -15°C, and protect from light. Literature: 1. H. Lowry, Micromethods for the assay of enzymes. Methods Enzymol. 1957, 4, 366. 2. R.J. Sugasawara, et al., Enzyme-linked immunosorbent assay with a monoclonal antibody for detecting group A meningococcal antigens in cerebrospinal fluid. J. Clin. Microbiol. 1984, 19, 230. View product page →
Specifications
CAS Number 333338-18-4
Technical Resources
5-Fluoroorotic acid monohydrate (5-FOA)
Catalog ID
Size
Pricing
More Info
5-Fluoroorotic acid monohydrate (5-FOA) product image
F-230-2.5
2.5 g
$ 54.00
F-230-5
5 g
$ 103.00
F-230-10
10 g
$ 173.00
F-230-25
25 g
$ 318.00
Description

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

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Specifications
CAS Number 220141-70-8
Technical Resources
50 bp DNA Ladder
Catalog ID
Size
Pricing
More Info
50 bp DNA Ladder product image
D100-500
1x500 μl
$ 55.00
D100-1000
2x500 μl
$ 103.00
D100-1500
3x500 μl
$ 147.00
D100-2000
4x500 μl
$ 185.00
D100-2500
5x500 μl
$ 217.00
D100-3000
6x500 μl
$ 239.00
Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 17 fragments, suitable for gel electrophoresis. The DNA includes fragments ranging from 50-1,500 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 g a load) for approximating the mass of DNA in comparably intense samples of a similar size.The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

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Specifications
CAS Number
Technical Resources
AEBSF
Catalog ID
Size
Pricing
More Info
AEBSF product image
A-540-500
500 mg
$ 78.00
A-540-1
1 g
$ 129.00
A-540-2.5
2.5 g
$ 260.00
A-540-5
5 g
$ 394.00
A-540-10
10 g
$ 650.00
A-540-25
25 g
$ 1375.00
Description

AEBSF is an irreversible serine protease inhibitor. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein and thrombin. It inhibits by acylation of the active site of the enzyme. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25 mM. Aqueous solutions are stable between pH 5-6; limited stability above pH 7.5.

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Specifications
CAS Number 30827-99-7
Technical Resources
Agarose LE (Molecular Biology Grade)
Catalog ID
Size
Pricing
More Info
Agarose LE (Molecular Biology Grade) product image
A-201-100
100 g
$ 86.00
A-201-500
500 g
$ 318.00
A-201-1000
1 kg
$ 530.00
Description

Agarose LE (Low Electroendosmosis) is Molecular Biology Grade and used for the superior separation of nucleic acids and for the maximum crisp resolution of bands.

GoldBio Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end-product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 50 bp to 25 kbp, protein electrophoresis and various blotting protocols.

The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

Agarose is a natural product that forms an inert matrix used in electrophoresis, chromatography and other molecular biology and biochemistry techniques. Likewise, it is neutral and easily derivatizable, so it is easy to bind to its structure proteins like enzymes, antigens or antibodies. Toxicity absence makes working with agarose very convenient.

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Specifications
CAS Number 9012-36-6
Technical Resources
Ampicillin (Sodium), USP Grade
Catalog ID
Size
Pricing
More Info
Ampicillin (Sodium), USP Grade product image
A-301-5
5 g
$ 21.00
A-301-10
10 g
$ 32.00
A-301-25
25 g
$ 43.00
A-301-100
100 g
$ 162.00
A-301-500
500 g
$ 775.00
Description

Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains. Ampicillin sodium targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. It has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus species. Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation. Ampicillin Sodium is freely soluble in water.

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Specifications
CAS Number 69-52-3
Technical Resources
Aprotinin
Catalog ID
Size
Pricing
More Info
Aprotinin product image
A-655-25
25 mg
$ 43.00
A-655-100
100 mg
$ 108.00
Description

Aprotinin is a monomeric globular polypeptide derived from bovine lung tissue. It consists of a chain of 58 amino acids that fold into a stable, compact tertiary structure of the 'small SS-rich" type, containing 3 disulfides, a twisted β-hairpin and a C-terminal α-helix.

Aprotinin inhibits several serine proteases, specifically trypsin, chymotrypsin, plasmin and kallikrein. Its action on kallikrein leads to the inhibition of the formation of factor XIIa. Binding is reversible with most aprotinin-protease complexes dissociating at pH >10 or < 3. Aprotinin is freely soluble in water (>10 mg/ml) as well as low ionic strength aqueous buffers.

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Specifications
CAS Number 9087-70-1
Technical Resources
Bacterial Cell Lysis Buffer
Catalog ID
Size
Pricing
More Info
Bacterial Cell Lysis Buffer product image
GB-177-100
100 mL
$ 54.00
GB-177-500
500 mL
$ 106.00
GB-176-100
100 Preps
$ 152.00
GB-176-250
250 Preps
$ 268.00
GB-176-500
500 Preps
$ 495.00
Description
The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.Bacterial Cell Lysis Buffer is based on organic buffering agents and utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of View product page →
Specifications
CAS Number
Technical Resources
BCIP
Catalog ID
Size
Pricing
More Info
BCIP product image
B-500-10
10 g
$ 272.00
B-500-25
25 g
$ 675.00
B-500-50
50 g
$ 1281.00
B-500-100
100 g
$ 2443.00
Description

BCIP is a white to off-white powder that is used as a substrate for visualization of alkaline phosphatase. The end product is insoluble and purple when used with nitroblue tetrazolium. This final product can be visually detected. BCIP is DMF soluble.

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Specifications
CAS Number 6578-06-9
Technical Resources
Benzamidine hydrochloride monohydrate
Catalog ID
Size
Pricing
More Info
Benzamidine hydrochloride monohydrate product image
B-050-500
500 g
$ 222.00
B-050-1
1 kg
$ 371.00
B-050-2.5
2.5 kg
$ 773.00
B-050-5
5 kg
$ 1288.00
Description

Benzamidine hydrochloride monohydrate is an aromatic amidine. It is used in protein extractions as a strong reversible inhibitor of serine proteases such as trypsin, thrombin, plasmin and other trypsin-like proteases. Compared to aprotinin, benzamidine is considered to be just as effective in preventing the degradation of glucogon.

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Specifications
CAS Number 1670-14-0
Technical Resources
Biotin-Maleimide with Biotrace™
Catalog ID
Size
Pricing
More Info
Biotin-Maleimide with Biotrace™ product image
B-420-10
10 mg
$ 119.00
B-420-25
25 mg
$ 219.00
B-420-kit
1 kit
$ 295.00
Description
Biotin-Maleimide with Biotrace™ is a sulfhydryl-reactive, UV-Vis traceable biotinylation reagent that enables incorporation of biotin onto antibodies, cysteine-containing peptides and other thiol-containing molecules.This advanced biotinylation reagent contains a chromophore, UV-traceable benzophenone moiety with absorption maximum of 350 nm, surrounded by two long hydrophilic PEG4 spacer arms. The hydrophilic arm enhances water solubility while reducing the tendency of some proteins to aggregate/precipitate during biotinylation. Extended PEG spacers improve conjugate yield while reducing nonspecific interactions for enhanced assay signal at higher biotin loads. After biotinylation, the conjugate's absorbance at 280 nm and 350 nm is used to rapidly and quantitatively measure the View product page →
Specifications
CAS Number
Technical Resources
Biotin-NHS Ester with Biotrace™
Catalog ID
Size
Pricing
More Info
Biotin-NHS Ester with Biotrace™ product image
B-220-10
10 mg
$ 119.00
B-220-25
25 mg
$ 219.00
Description
Biotin-NHS Ester with Biotrace™ is an amine-reactive, UV-traceable biotinylation reagent that enables controllable and reproducible labeling of amine-containing biopolymers.This advanced biotinylation reagent contains a chromophore, UV-traceable benzophenone moiety with absorption maximum of 350 nm, surrounded by two long hydrophilic PEG4 spacer arms. The hydrophilic arm enhances water solubility while reducing the tendency of some proteins to aggregate/precipitate during biotinylation. Extended PEG spacers improve conjugate yield while reducing nonspecific interactions for enhanced assay signal at higher biotin loads. After biotinylation, the conjugate's absorbance at 280 nm and 350 nm is used to rapidly and quantitatively measure the level of biotin incorporation. View product page →
Specifications
CAS Number
Technical Resources
Biotin‐NHS ester
Catalog ID
Size
Pricing
More Info
Biotin‐NHS ester product image
B-120-100
100 mg
$ 95.00
B-120-1000
1000 mg
$ 195.00
Description
Biotin‐NHS ester reagent enables simple and efficient biotin labeling of amine-containing organic molecules, antibodies, proteins and any other primary amine-containing biomolecules in solution. NH2-activated biotins require an organic solvent such as DMSO or DMF to be dissolved. Formula: C14H19N3O5S MW: 341.38 g/mol Storage/Handling: Store at -20°C. Product shipped at ambient temperature. Soluble in DMSO or DMF Highlights: - Biotin‐NHS ester reacts rapidly with any primary amine-containing molecule to attach the biotin label via   a stable amide bond. - Biotin‐NHS ester is water insoluble - must be dissolved in DMSO or DMF before further dilution in   aqueous buffers. - View product page →
Specifications
CAS Number 35013-72-0
Technical Resources
Biotin‐PEG4‐NHS Ester
Catalog ID
Size
Pricing
More Info
Biotin‐PEG4‐NHS Ester product image
B-720-25
25 mg
$ 95.00
B-720-100
100 mg
$ 195.00
Description
Biotin‐PEG4‐NHS Ester is a pegylated, highly hydrophilic biotinylation reagent for simple and efficient biotin labeling of antibodies, proteins and other primary amine-containing biomolecules.Biotin‐PEG4‐NHS Ester reacts specifically and efficiently with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces) at pH 7-9 to form covalent bonds. The hydrophilic polyethylene glycol (PEG) spacer arm of this reagent imparts water solubility that is transferred to the labeled molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG spacer arm also gives the reagent a long and flexible connection to minimize steric hindrance involved with binding to avidin molecules. Formula: View product page →
Specifications
CAS Number
Technical Resources
BLUEstain™ 2 Protein ladder, 5-245 kDa
Catalog ID
Size
Pricing
More Info
BLUEstain™ 2 Protein ladder, 5-245 kDa product image
P008-500
1x500 μl
$ 109.00
P008-1000
2x500 μl
$ 212.00
P008-1500
3x500 μl
$ 303.00
P008-2000
4x500 μl
$ 394.00
P008-2500
5x500 μl
$ 478.00
P008-3000
6x500 μl
$ 558.00
Description

The BLUEstain™ 2 Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). 


The BLUEstain™ 2 Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.

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