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TCEP-HCl is an odorless reducing agent suitable for protein applications. The compound has many desirable features, some of which are as follows:
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• TCEPHCl is stable in air.
• TCEPHCl is odorless so reactions can be carried out on the bench without the need for a fume hood.
• TCEPHCl is efficient and can be used to reduce disulfide bonds at low concentrations.
• TCEPHCl is selective for disulfides and does not react with other protein sidechains.
• TCEPHCl may be used as a substitute for DTT at a final concentration of 50 mM.