RT-PCR & QPCR Kits

GoldBio has an assortment of kits for RT-PCR, QPCR, and RT-QPCR to maximize and provide consistent and robust transcriptions and amplifications. Our polymerase offers the highest fidelity and best amplification available.

Showing 1-8 of 8 items.

Goof-Proof™ qPCR Master Mix

Catalog ID Size Pricing
G-700-1 1 mL $ 63.00
G-700-5 5 x 1 mL $ 216.00

Description

Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue (see Figure 2).

Goof-Proof™ allows you to see quickly and easily whether you forgot to add the master mix or the template to any of your reactions, preventing pipetting mistakes and wasted time, reagents and money. The 2X Goof-Proof™ Master Mix can also be used without the Template Buffer, if desired.

Product Specifications

Catalog ID G-700
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G-700-1 1 mL $ 63.00
G-700-5 5 x 1 mL $ 216.00

Goof-Proof™ qPCR Master Mix with ROX

Catalog ID Size Pricing
G-705-1 1 mL $ 63.00
G-705-5 5 x 1 mL $ 216.00

Description

Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue.

Product Specifications

Catalog ID G-705
Storage/Handling Store the kit at -20°C.
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G-705-1 1 mL $ 63.00
G-705-5 5 x 1 mL $ 216.00

One Step RT-PCR Kit

There is no image for One Step RT-PCR Kit One Step RT-PCR Kit
Catalog ID Size Pricing
R-910-100 100 reactions $ 180.00
R-910-500 500 reactions $ 680.00

Description

GoldBio's One Step RT-PCR kit combines two powerful mixtures: i) Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.

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R-910-100 100 reactions $ 180.00
R-910-500 500 reactions $ 680.00

One Step RT-qPCR Kit

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Catalog ID Size Pricing
R-915-100 100 reactions $ 195.00
R-915-500 500 reactions $ 695.00

Description

One step RT-qPCR kit combines two powerful mixtures: i) Reverse Transcriptase and ii) SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.

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R-915-100 100 reactions $ 195.00
R-915-500 500 reactions $ 695.00

One Step RT-qPCR kit for SARSCoV-2 (COVID-19) Detection

There is no image for One Step RT-qPCR kit for SARSCoV-2 (COVID-19) Detection One Step RT-qPCR kit for SARSCoV-2 (COVID-19) Detection
Catalog ID Size Pricing
P-065-100 100 reactions $ 576.00
P-065-500 500 reactions $ 2,583.00

Description

The One Step RT-qPCR kit is used for detection of SARS-CoV-2 in vitro. The approach used for detection is by Real-Time quantitative PCR (RT-qPCR). The SARS-CoV-2 detection kit allows cDNA synthesis and qPCR in a single tube. The kit provides an improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. In addition, the kit contains CDC recommended primers/probe sets for SARS-CoV-2 and can be used to detect SARS-CoV-2 in respiratory specimens; such as sputum, nasopharyngeal, orophayringel aspirates, washes or swabs and tracheal aspirates.

Product Specifications

Catalog ID P-065
Storage/Handling Store the kit at -20°C.
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P-065-100 100 reactions $ 576.00
P-065-500 500 reactions $ 2,583.00

qPCR Master Mix with SYBR Green®

There is no image for qPCR Master Mix with SYBR Green® qPCR Master Mix with SYBR Green®
Catalog ID Size Pricing
M-915-100 100 reactions $ 71.00
M-915-500 500 reactions $ 236.00

Description

GoldBio's qPCR 2X Master Mix with SYBR Green® is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The master mix contains integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

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M-915-100 100 reactions $ 71.00
M-915-500 500 reactions $ 236.00

RT-PCR Kit

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Catalog ID Size Pricing
R-920-50 50 Reactions $ 173.00
R-920-200 200 Reactions $ 638.00

Description

RT-PCR Kit combines two powerful mixtures: i) 5x master mix and ii) Hot start Taq 2x master mix with standard buffer for 2-steps RT-PCR. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.

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R-920-50 50 Reactions $ 173.00
R-920-200 200 Reactions $ 638.00

RT-qPCR Kit

There is no image for RT-qPCR Kit RT-qPCR Kit
Catalog ID Size Pricing
R-925-50 50 reactions $ 206.00
R-925-200 200 reactions $ 692.00

Description

RT-qPCR kit combines two powerful mixtures: i) first strand cDNA synthesis kit and ii) SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.

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R-925-50 50 reactions $ 206.00
R-925-200 200 reactions $ 692.00

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