Affinity Reagents

Gold Biotechnology gravity flow empty columns are ideal for normal protein purification processes. Customers may buy bulk resins and then pack their own His-tag purification columns as an economic option.

These columns serve as tools for gravity purification using small quantities of resin. The package contains 50 plastic columns with their top and end caps.

The plastic columns are polypropylene with a total volume of either 12 mL or 35 mL and contain a polyethylene frit with a nominal pore size of 20 µm. They are specially designed for working with bead volumes of 0.5-2.0 mL (12 mL column) or 2-6 mL (35 mL column).

Gold Biotechnology gravity flow, Empty Spin Mini Columns are great for small quantity protein purification processes. Customers may buy bulk resins and then pack their own His-tag purification columns as an economic option.

This column serves as a tool for purification using small quantities of resin (100 – 250 µl). It is a single-use format for centrifuge purification.

Gold Biotechnology gravity flow, Empty Spin Columns are an excellent option for small quantity protein purification processes. Customers may buy bulk resins and then pack their own his tag purification columns as an economic option.

The Spin Column serves as a tool for purification using small quantities of resin (50 – 100 µl). It is a single-use format for purifications with syringe (luer lock system) or by centrifugation.

Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.

Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).

Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).

Protein A Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein A. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

Protein A Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.

GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.

Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.

Protein G Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein G. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

Protein G Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

Protein L Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification and immunoprecipitation. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein L. Magnetic agarose beads provide a convenient and quick method for purifications of IgG, IgM, IgA, and IgD containing kappa light chains from various species without interfering with the antigen binding site.

Protein L Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

Streptavidin Agarose Resin is a high affinity biotin-binding chromatography resin with streptavidin covalently bound to 6% crosslinked agarose beads and a biotin binding capacity of >120 nmol/ml of gel. Immobilized Streptavidin resin is suitable for gravity flow columns, spin columns, and FPLC methods. Immobilized Streptavidin can be used for affinity chromatography purifications by separating biotinylated proteins from nonbiotinylated proteins and for affinity purification of antigens when used with biotinylated antibodies.