Affinity Reagents
Affinity reagents and affinity-based tools utilize complex networks of specific interactions between various molecules for use in the separation, purification or analysis of proteins. Affinity purification requires an affinity ligand to be coupled to a matrix, which allows capture of the intended product from a complex mixture. Gold Biotechnology’s affinity reagents are activated beads or preactivated resins for use in coupling of affinity ligands and immobilization. Our affinity beads are specifically engineered to meet your specialized needs, whether it be laboratory scale experiments or industry scale separations. GoldBio’s agarose beads are conveniently cross-linked to include any of the following: aminoethyl or glyoxal groups, glutathione S-transferase, Protein A, Protein G, streptavidin and His-tag metal ions such as nickel, cobalt, copper or zinc.
Sub-Categories
Plastic Columns
Catalog ID | Size | Pricing | |
---|---|---|---|
P-301 | 50 x 12 mL | $ 317.00 | |
P-302 | 50 x 35 mL | $ 392.00 |
Description
Gold Biotechnology gravity flow empty columns are ideal for normal protein purification processes. Customers may buy bulk resins and then pack their own His-tag purification columns as an economic option.
These columns serve as tools for gravity purification using small quantities of resin. The package contains 50 plastic columns with their top and end caps.
The plastic columns are polypropylene with a total volume of either 12 mL or 35 mL and contain a polyethylene frit with a nominal pore size of 20 µm. They are specially designed for working with bead volumes of 0.5-2.0 mL (12 mL column) or 2-6 mL (35 mL column).
Plastic Mini Columns
Catalog ID | Size | Pricing | |
---|---|---|---|
P-307-25 | 1 x 25 each | $ 135.00 | |
P-307-100 | 1 x 100 each | $ 492.00 |
Description
Gold Biotechnology gravity flow, Empty Spin Mini Columns are great for small quantity protein purification processes. Customers may buy bulk resins and then pack their own His-tag purification columns as an economic option.
This column serves as a tool for purification using small quantities of resin (100 – 250 µl). It is a single-use format for centrifuge purification.
Product Specifications
Catalog ID | P-307 |
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Plastic Spin Columns
Catalog ID | Size | Pricing | |
---|---|---|---|
P-300 | 800 μL | $ 225.00 |
Description
Gold Biotechnology gravity flow, Empty Spin Columns are an excellent option for small quantity protein purification processes. Customers may buy bulk resins and then pack their own his tag purification columns as an economic option.
The Spin Column serves as a tool for purification using small quantities of resin (50 – 100 µl). It is a single-use format for purifications with syringe (luer lock system) or by centrifugation.
Product Specifications
Catalog ID | P-300 |
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Protein A Agarose Resin (Pharmaceutical grade for research)
Catalog ID | Size | Pricing | |
---|---|---|---|
P-400-5 | 5 mL | $ 195.00 | |
P-400-25 | 25 mL | $ 755.00 | |
P-400-50 | 50 mL | $ 1,385.00 | |
P-400-100 | 100 mL | $ 2,349.00 |
Description
Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.
Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).
Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).
Product Specifications
Catalog ID | P-400 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Protein A Magnetic Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
P-404-2 | 2 mL | $ 120.00 | |
P-404-5 | 5 mL | $ 284.00 |
Description
Protein A Magnetic Agarose Beads are designed to provide the easiest
and simplest method of monoclonal and polyclonal antibody purification.
The antibodies are retained to the magnetic resin which are also
covalently conjugated with recombinant Protein A. Magnetic agarose beads
provide a convenient and quick method for purification without the need
for pipetting or centrifugation. Washing, binding and elution steps
will require a magnetic device.
Protein A Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)
Binding Capacity: ~10 mg human IgG / ml resin.
Product Specifications
Catalog ID | P-404 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Protein G HTC Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
P-430-5 | 5 mL | $ 375.00 | |
P-430-25 | 25 mL | $ 1,306.00 |
Description
Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.
GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.
Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.
Product Specifications
Catalog ID | P-430 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Protein G Magnetic Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
P-434-2 | 2 mL | $ 132.00 | |
P-434-5 | 5 mL | $ 300.00 |
Description
Protein G Magnetic Agarose Beads are designed to provide the easiest
and simplest method of monoclonal and polyclonal antibody purification.
The antibodies are retained to the magnetic resin which are also
covalently conjugated with recombinant Protein G. Magnetic agarose
beads provide a convenient and quick method for purification without
the need for pipetting or centrifugation. Washing, binding and elution
steps will require a magnetic device.
Protein G Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)
Binding Capacity: ~10 mg human IgG / ml resin.
Product Specifications
Catalog ID | P-434 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |
Protein L Magnetic Agarose Beads
Catalog ID | Size | Pricing | |
---|---|---|---|
P-454-2 | 2 mL | $ 138.00 | |
P-454-5 | 5 mL | $ 325.00 |
Description
Protein L Magnetic Agarose Beads are designed to provide the easiest
and simplest method of monoclonal and polyclonal antibody purification and immunoprecipitation.
The antibodies are retained to the magnetic resin which are also
covalently conjugated with recombinant Protein L. Magnetic agarose
beads provide a convenient and quick method for purifications of IgG, IgM, IgA, and IgD containing kappa light chains
from various species without interfering with the antigen binding site.
Protein L Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)
Binding Capacity: ~10 mg human IgG / ml resin.
Product Specifications
Catalog ID | P-454 |
---|---|
Storage/Handling | Store at 4°C. Do not freeze. |
Streptavidin Agarose Resin
Catalog ID | Size | Pricing | |
---|---|---|---|
S-105-1 | 1 mL | $ 79.00 | |
S-105-5 | 5 mL | $ 289.00 | |
S-105-10 | 10 mL | $ 505.00 |
Description
Streptavidin Agarose Resin is a high affinity biotin-binding chromatography resin with streptavidin covalently bound to 6% crosslinked agarose beads and a biotin binding capacity of >120 nmol/ml of gel. Immobilized Streptavidin resin is suitable for gravity flow columns, spin columns, and FPLC methods. Immobilized Streptavidin can be used for affinity chromatography purifications by separating biotinylated proteins from nonbiotinylated proteins and for affinity purification of antigens when used with biotinylated antibodies.
Product Specifications
Catalog ID | S-105 |
---|---|
Storage/Handling | store at 4°C. Do NOT freeze. |