Aminoethyl Agarose Beads

Gold Biotechnology’s aminoethyl agarose resins are cross-linked, autoclavable agarose beads used for protein immobilization. Our resins are covalently bound to the agarose, giving them a qualitative advantage to resins activated by CNBr. These agarose resins have a higher binding capacity, faster conjugation, more reproducibility and a longer shelf life than CNBr. Aminoethyl agarose resins are safer to use and are designed to bind with the carboxy groups on a protein with high specificity. GoldBio offers both low pressure resins (low density) for R&D scale experiments as well as high pressure resins (high density) for industrial scale separations. For best results, screen your proteins with the one of our aminoethyl test kits in order to optimize the activation of your specific protein.
Showing 1-9 of 9 items.

Aminoethyl (High Density) 4% Agarose Beads, Crosslinked

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Catalog ID Size Pricing
A-499-25 25 mL $ 146.00
A-499-100 100 mL $ 502.00

Description

High Density Aminoethyl 4% crosslinked beads allows covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in batch or column purifications (Low Pressure). The resin has a high amount of aldehyde groups (40-60 µm diaminoethyl / mL gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand. The backbone of the resin is 4% agarose beads and the pore size is adequate to work with enzymes, proteins & antibodies.

Aminoethyl resins give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Product Specifications

Catalog ID A-499
Storage/Handling store at 4°C. Do NOT freeze.
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A-499-25 25 mL $ 146.00
A-499-100 100 mL $ 502.00

Aminoethyl (High Density) 6% Agarose Beads, Crosslinked

There is no image for Aminoethyl (High Density) 6% Agarose Beads, Crosslinked Aminoethyl (High Density) 6% Agarose Beads, Crosslinked
Catalog ID Size Pricing
A-503-25 25 mL $ 151.00
A-503-100 100 mL $ 556.00

Description

HIGH Density Aminoethyl 6% crosslinked beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in batch or column purifications (Low Pressure). The resin has a high amount of aldehyde groups (40-60 µm diaminoethyl / mL gel) providing a very good option to conjugate affinity ligands improving the stability of the ligand. The backbone of the resin is 6% agarose beads and the pore size is adequate to work with enzymes & proteins mainly.

Aminoethyl resins give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Product Specifications

Catalog ID A-503
Storage/Handling store at 4°C. Do NOT freeze.
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A-503-25 25 mL $ 151.00
A-503-100 100 mL $ 556.00

Aminoethyl (Low Density) 6% Agarose Beads, Crosslinked

Catalog ID Size Pricing
A-501-25 25 mL $ 146.00
A-501-100 100 mL $ 483.00

Description

LOW Density Aminoethyl 6% crosslinked beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in batch or column purifications (Low Pressure). This resin has a low content of aldehyde groups (15- 25 µm diaminoethyl / ml gel) providing a very good option to conjugate affinity ligands without distorting the target ligand. The backbone of the resin is 6% agarose beads and the pore size is adequate to work with enzymes & proteins mainly.

Aminoethyl resin give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.

Product Specifications

Catalog ID A-501
Storage/Handling store at 4°C. Do NOT freeze.
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A-501-25 25 mL $ 146.00
A-501-100 100 mL $ 483.00

Aminoethyl (Very Low Density) 4% Agarose Beads, Crosslinked

Catalog ID Size Pricing
A-500-25 25 mL $ 139.00
A-500-100 100 mL $ 476.00

Description

Biomolecules can be immobilized by binding them to a support under conditions that will then determine the characteristics of the complex. Immobilization is a technique that binds a ligand (enzyme, antibody, affinity proteins like Protein A or Protein G) to a support, giving high stability and making it easier to reuse the immobilized molecule.

Aminoethyl resin give the biomolecules increased stability through the covalent bonds of the agarose to carboxy group amino acids of the target ligand, thus facilitating recovery and later reuse. This covalent binding also confers a qualitative advantage compared to resins activated with CNBr, in which the binding is weaker.

The choice of Glyoxal or Aminoethyl will depend on the biomolecule to be immobilized, the accessibility of the reactive groups, and the direction/ orientation required for the binding to the support.

Product Specifications

Catalog ID A-500
Storage/Handling store at 4°C. Do NOT freeze.
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A-500-25 25 mL $ 139.00
A-500-100 100 mL $ 476.00

Aminoethyl HTC 4% HIGH Density Agarose Beads

There is no image for Aminoethyl HTC 4% HIGH Density Agarose Beads Aminoethyl HTC 4% HIGH Density Agarose Beads
Catalog ID Size Pricing
A-504-25 25 mL $ 160.00
A-504-100 100 mL $ 551.00

Description

Aminoethyl HTC 4% HIGH Density beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in large scale process (High Pressure). The resin has a high amount of amino groups (40-60 µm diaminoethyl / mL gel) providing multiple binding points and high immobilized ligand stability. The resin has been manufactured using 4% HTC agarose beads, and the pore size is adequate to work with enzymes, proteins & antibodies. The stability at high flow is adequate for large scale process.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-504
Storage/Handling store at 4°C. Do NOT freeze.
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A-504-25 25 mL $ 160.00
A-504-100 100 mL $ 551.00

Aminoethyl HTC 4% VERY LOW Density Agarose Beads

There is no image for Aminoethyl HTC 4% VERY LOW Density Agarose Beads Aminoethyl HTC 4% VERY LOW Density Agarose Beads
Catalog ID Size Pricing
A-505-25 25 mL $ 153.00
A-505-100 100 mL $ 524.00

Description

Aminoethyl HTC 4% VERY LOW Density beads allow a covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in large scale process (High Pressure). The resin has a low content of amino groups (3- 6 µm diaminoethyl / mL gel) providing a minimum distortion of conjugated affinity ligand. The resin has been manufactured using 4% HTC agarose beads, and the pore size is adequate to work with enzymes, proteins & antibodies. The stability at high flow is adequate for large scale process.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-505
Storage/Handling store at 4°C. Do NOT freeze.
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A-505-25 25 mL $ 153.00
A-505-100 100 mL $ 524.00

Aminoethyl HTC 6% HIGH Density Agarose Beads

There is no image for Aminoethyl HTC 6% HIGH Density Agarose Beads Aminoethyl HTC 6% HIGH Density Agarose Beads
Catalog ID Size Pricing
A-507-25 25 mL $ 167.00
A-507-100 100 mL $ 556.00

Description

Aminoethyl 6% HIGH Density beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in large scale process (High Pressure). The resin has a high amount of amino groups (40-60 µm diaminoethyl / mL gel) providing multiple binding points and high immobilized ligand stability. The resin has been manufactured using 6% HTC agarose beads, and the pore size is adequate to work with enzymes, proteins & small ligands. The stability at high flow is adequate to large scale process.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-507
Storage/Handling store at 4°C. Do NOT freeze.
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A-507-25 25 mL $ 167.00
A-507-100 100 mL $ 556.00

Aminoethyl HTC 6% LOW Density Agarose Beads

There is no image for Aminoethyl HTC 6% LOW Density Agarose Beads Aminoethyl HTC 6% LOW Density Agarose Beads
Catalog ID Size Pricing
A-506-25 25 mL $ 159.00
A-506-100 100 mL $ 478.00

Description

Aminoethyl HTC 6% LOW Density beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. This product is adequate to work in large scale process (High Pressure). The resin has a low content of amino groups (15- 25 µm diaminoethyl / mL gel) providing a minimum distortion of conjugated affinity ligand. The resin has been manufactured using 6% HTC agarose beads and the pore size is adequate to work with enzymes, proteins & small ligands. The stability at high flow is adequate for large scale process.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-506
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-506-25 25 mL $ 159.00
A-506-100 100 mL $ 478.00

Aminoethyl HTC 6% VERY LOW Density Agarose Beads

There is no image for Aminoethyl HTC 6% VERY LOW Density Agarose Beads Aminoethyl HTC 6% VERY LOW Density Agarose Beads
Catalog ID Size Pricing
A-508-25 25 mL $ 170.00
A-508-100 100 mL $ 579.00

Description

Aminoethyl HTC 6% VERY LOW Density beads allow covalent binding of agarose to carboxy group amino acids of the target ligand. The resin has a low content of amino groups (3- 6 µm diaminoethyl / mL gel) providing a minimum distortion of conjugated affinity ligand. The resin has been manufactured using 6% HTC FINE agarose beads, and the pore size is adequate to work with enzymes, proteins & small ligands. The bead size is adequate to manufacture resins for packing cartridges.

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID A-508
Storage/Handling store at 4°C. Do NOT freeze.
View
Quick Add
A-508-25 25 mL $ 170.00
A-508-100 100 mL $ 579.00

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