Analytical Reagents

Spermidine trihydrochloride is a polyamine that inhibits neuronal nitric oxide synthase (nNOS) and binds and precipitates DNA. It may be used to purify DNA binding proteins. Additionally, spermidine stimulates T4 polynucleotide kinase activity. It is involved in growth, development, and the stress response in plants.

Spermine is a polyamine derived from spermidine. It is involved in the cellular metabolism of all eukaryotic cells, as well as being vital to some bacteria.

Due to its polycationic nature, nonviral gene therapy applications involving spermine have shown promising results. This has had a positive impact in cancer research involving the lungs.

Gene transfer within the lungs is met with many obstacles due to inflammatory and immunological responses. Using spermine as a gene delivery vehicle to transfect living cells has bypassed some of these obstacles, showing higher gene expression.

Dimethylsulfoxide (DMSO) is a very polar organic compound that is useful in organic chemistry for solubilizing organic and inorganic compounds. DMSO is also used as a cryoprotectant for cell media, preventing the formation of ice crystals which would otherwise damage cells. DMSO can be used in PCR reactions to inhibit the self-complementation of DNA or PCR primers and increase amplification, especially for DNA sequences which have GC rich sequences.

GoldBio's DMSO is filter sterilized through a 0.2 μg filter. Sterile DMSO is ideal for working with tissue culture or cell culture in which there is a need for eliminating all potential sources of contamination.

Trans-Stilbene is used in transition metal-catalyzed asymmetric epoxidation and dihydroxylation. It is also useful in making synthetic estrogens.

GoldBio Tris has the highest purity available on the market. Short for Tris (hydroxymethyl) aminomethane (THAM), Tris is an organic compound often used in buffer solutions such as TAE or TBE for electrophoresis gels. Tris is highly soluble in water and is useful in the pH range 7.0-9.0 and is also used in the preparation of Laemmli buffer, one of the most common SDS-PAGE buffers. In addition, it can also be used for many custom running and loading buffers, most often with glycine and SDS.

Tris hydrochloride is a reagent that is used for the stabilization of buffer formulations and electrophoresis of biological molecules.

XTT sodium salt is used in the XTT colormetric assay and assists in identifying metabolic activity within cells. In the XTT assay, the metabolic activity of cells reduces XTT sodium salt to an orange color formazan dye by dehydrogenase enzymes. Dye conversion will only occur in viable cells.