B_Products

5X Passive Lysis Buffer is a concentrated cell lysis buffer that is designed for use with GoldBio’s Illumination™ Renilla Luciferase Enhanced Assay Kit.The kit is supplied with enough lysis buffer to perform the stated number of assays.This 5X Passive Lysis Buffer is sold separately for use with the kits when more lysis buffer is required.

5X Passive Lysis Buffer is not compatible with Firefly Luciferase or the Illumination™ Luciferase Enhanced Assay Kit.For Firefly luciferase assays, use 5X Luciferase Lysis Buffer (GoldBio Catalog # L-740).

This Buffer Set consists of:

- 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)
- 25 mL 3M Recrystallized Imidazole Solution, pH 6.0

This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.

5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).

Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.

The plant hormones cytokinins (CKs) comprise a class of growth regulators involved in the stress response, senescence, photosynthesis, nutrient assimilation and mobilization, as well as modulation of a plant tissue’s ability to act as a sink or source of metabolites.

CKs are recognized by a subset of sensor histidine kinases (HKs) and the signal is transmitted to the nucleus via a multistep phosphorelay (MSP), thus activating the expression of target genes

6-Benzyloxyindole-3-carboxaldehyde is a cyclic reagent for the creation of organic molecules like substituted naphthylene leukotriene B4 antagonists which are useful as anti-inflammatory agents.

6-Bromo-2-naphthyl-alpha-D-mannopyranoside is a substrate for alpha-mannosidase. By binding with a staining reagent a red-brown dye is formed.

6-Bromo-DL-tryptophan is an analog of tryptophan.

GoldBio's 6x Blue DNA Loading Dye is pre-mixed buffer designed for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 4,000 bp and 500 bp on a standard 1% TAE agarose gel respectively (800 bp and 150 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

GelRed™ is an ultra-sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed™ Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.

6X GelRed™ Prestain Loading Buffer with Orange Tracking Dye contains an orange electrophoresis tracking dye that run at approximately 50 bp in a 1% agarose gel.

GelRed™ and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed™ is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed™ is compatible with downstream applications such as sequencing and cloning. GelRed™ is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.

7-Benzyloxyindole is a reagent for the preparation of inhibitors like HCV inhibitor and pyrazolo quinolone derivatives that function as adenosine receptor antagonists.

7-Bromoindole is a reagent for chemical synthesis.

alpha,beta-D-Glucose pentaacetate is a reagent used for chemical synthesis. It can be used to induce the secretion of insulin.

Bacitrac99 is the most pure form of bacitracin available, and should be used for the most sensitive applications and on the most sensitive cell lines since any impurities that might cause inhibition of resistant cell lines have been removed. In general, our USP Grade bacitracin will not adversely affect your cells and is suitable for most experiments.

Bacitracin is a cyclic polypeptide antibiotic isolated from Bacillus subtilis. It is made up of a variety of antimicrobial peptide fractions including A, A1, B, B1, B2, C, D, E, F, G, and X. This standard grade product is a mixture of these fractions, with bacitracin A exhibiting the most potent antimicrobial activity. Bacitracins B1 and B2 exhibit about 90% of the antimicrobial activity of bacitracin A when together. Other forms of bacitracin have not been studied extensively, but may have negligible antimicrobial properties.

Bacitracin targets the cell wall of gram-positive organisms such as Streptococcus pyogenes and Staphylococcus aureus. Bacitracin also functions as a useful tool to distinguish between group A and group B β-hemolytic Streptococcus species, which are susceptible and resistant, respectively.

Bacitracin is a cyclic polypeptide antibiotic isolated from Bacillus subtilis. It is made up of a variety of antimicrobial peptide fractions including A, A1, B, B1, B2, C, D, E, F, G, and X. This standard grade product is a mixture of these fractions, with bacitracin A exhibiting the most potent antimicrobial activity. Bacitracins B1 and B2 exhibit about 90% of the antimicrobial activity of bacitracin A when together. Other forms of bacitracin have not been studied extensively, but may have negligible antimicrobial properties. Bacitracin is freely soluble in aqueous solution.

Bacitracin targets the cell wall of gram-positive organisms such as Streptococcus pyogenes and Staphylococcus aureus. Bacitracin also functions as a useful tool to distinguish between group A and group B β-hemolytic Streptococcus species, which are susceptible and resistant, respectively.

Bactenecin is a twelve residue antimicrobial peptide that is recognized by its disulfide linked cyclical structure. It lyses gram-negative bacteria by increasing permeability through binding lipopolysaccharides (LPS). The bactenecin/LPS conjugate forms a β-hairpin structures which integrate with the bacterial membrane, forming pores. Cytosol contents leak with pore formation and lysis occurs with higher bactenecin concentrations

Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Baicalin is a flavone glycoside formed from the glucuronidation of baicalein. It has an array of properties which include promoting cell survival and bolstering the immune system. One of the effects that baicalin produced with in vitro studies of pathogens and in vivo studies on rats and mice was an elevation in cell survival, which implied the prevention of cell death due to induced apoptosis or the accumulation of oxidative species. Apoptotic repression occurred in rats due to a decreased expression of proteins within the CHOP (C/EBP homologous protein) pathway. Stimulating the synthesis of endothelial nitrogen oxides (eNOS) and other nitrogen oxides quell oxidative stress with the termination of free radicals.

Additionally, protective effects of baicalin have specifically diminished injury in the hippocampal area. Baicalin has been shown to stimulate the expression of Bcl-2 (B-cell lymphoma-2) which regulates apoptosis. It has also been shown to decrease miR-497 expression and cleave caspase-3 protein synthesis. These play roles in regulating gene expression and nervous system cell apoptosis respectively.

Enhancing the immune response with baicalin has proven effective in two ways. One observed effect was the induction of IFN-γ cytokine production in mice, a cytokine which activates macrophages and MHC II (major histocompatibility complex II) expression. Lysozyme bacteriostatic duration was shown to increase when exposed to increasing concentrations of baicalin.

Bakuchiol is a terpenophenolic compound extracted from the plant Psoralea corylifolia. Bakuchiol has been used as an oral antibiotic, anti-tumor agent and for the protection of liver and bone tissue. It has also been used as an insulin enhancer to counter hyperglycemia and hyperlipidemia for in vivo research on mice and rats. Within these studies, bakuchiol inhibited phospho-tyrosine protein phosphatase type 1B (PTP1B), DNA polymerase and topoisomerase II. Inactivity of these three enzymes greatly reduced PTPB1 activity, which has been found to suppress insulin signaling.

Antibiotic and anti-tumor uses of bakuchiol have been shown to be correlated to suppression of DNA synthesis. As a bactericidal agent, bakuchiol has been proven to be robust in vitro and is functional at many pH values as well as in the presence of organic acids. Bakuchiol terminates bacteria and malignant cells by generating intracellular reactive oxygen species (ROS), dismantling bacterial cells and compromising tumor cell mitochondria. Conversely, bakuchiol has also been shown to preserve liver function by inhibiting ROS that cause lipid peroxidation and the use of glutathione, an antioxidant.

The bone preserving effects of bakuchiol are related to its high binding affinity for estrogen receptor alpha (ERα). It has been shown that bakuchiol stimulates ERα to promote bone protective pathways.The pathways controlled by ERα increase alkaline phosphatase, calcium concentration, estradiol concentration and a decrease in inorganic phosphate level.

FGF2 is a member of the fibroblast growth factor family of proteins. It is often called FGF-basic or bFGF. FGF2 is a single-chain polypeptide growth factor that plays a significant role in wound healing, it is a potent inducer of angiogenesis and it is essential for the growth of many stem and progenitor cells. FGF2 shares a 55% amino acid residue identity with FGF1 and interacts with heparin sulfate with nanomolar affinity. It is a potent inducer of DNA synthesis in a variety of cell types. FGF2 activates mesenchymal splice variants of FGF receptors (c splice variants) and also activates FGF Receptor 1b, but has little activity against FGFR2b or FGFR3b. It is most closely associated with FGF1 (aFGF) (see additional information).