B_Products

Showing 121-128 of 128 items.

ProBlock™ Gold Bacterial Protease Inhibitor Cocktail [100X]

Catalog ID Size Pricing
GB-330-1 1 mL $ 85.00
GB-330-5 5 mL $ 295.00

Description

ProBlock™ Gold Bacterial Protease Inhibitor Cocktail contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of ProBlock™ Gold Bacterial inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Bacterial is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

Product Specifications

Catalog ID GB-330
Storage/Handling Store at 4°C.
View
Packs & Pricing
,
GB-330-1 In stock 1 mL $ 85.00
GB-330-5 In stock 5 mL $ 295.00

Protein L Magnetic Agarose Beads

There is no image for Protein L Magnetic Agarose Beads Protein L Magnetic Agarose Beads
Catalog ID Size Pricing
P-454-2 2 mL $ 138.00
P-454-5 5 mL $ 325.00

Description

Protein L Magnetic Agarose Beads are designed to provide the easiest and simplest method of monoclonal and polyclonal antibody purification and immunoprecipitation. The antibodies are retained to the magnetic resin which are also covalently conjugated with recombinant Protein L. Magnetic agarose beads provide a convenient and quick method for purifications of IgG, IgM, IgA, and IgD containing kappa light chains from various species without interfering with the antigen binding site.

Protein L Magnetic Beads are supplied as a 50% suspension of magnetic beads in 20% ethanol (Each 1000 μl of suspension contains 500 μl of magnetic beads.)

Binding Capacity: ~10 mg human IgG / ml resin.

Product Specifications

Catalog ID P-454
Storage/Handling Store at 4°C. Do not freeze.
View
Packs & Pricing
,
P-454-2 2-3 Weeks 2 mL $ 138.00
P-454-5 2-3 Weeks 5 mL $ 325.00

S-Butyrylthiocholine iodide

Catalog ID Size Pricing
B-820-10 10 g $ 131.00
B-820-25 25 g $ 276.00
B-820-50 50 g $ 524.00
B-820-100 100 g $ 814.00

Description

No description available

Product Specifications

Catalog ID B-820
CAS # 1866-16-6
MW 317.23 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
View
Packs & Pricing
,
B-820-10 2-3 Weeks 10 g $ 131.00
B-820-25 2-3 Weeks 25 g $ 276.00
B-820-50 2-3 Weeks 50 g $ 524.00
B-820-100 2-3 Weeks 100 g $ 814.00

Tissue Cell Lysis Buffer

Catalog ID Size Pricing
GB-181-100 100 mL $ 75.00
GB-181-500 500 mL $ 145.00

Description

Tissue Cell Lysis Buffer has been developed for extraction of total soluble protein from fresh or frozen animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue Cell Lysis Buffer. Tissue Cell Lysis Buffer reagent has been tested for use with a wide variety of animal tissues.

Product Specifications

Catalog ID GB-181
Storage/Handling Store at 4°C.
View
Packs & Pricing
,
GB-181-100 1 week 100 mL $ 75.00
GB-181-500 In stock 500 mL $ 145.00

Xylenol blue

Catalog ID Size Pricing
X-040-50 50 g $ 356.00
X-040-100 100 g $ 622.00
X-040-250 250 g $ 1,288.00
X-040-500 500 g $ 2,131.00

Description

Xylenol Blue is a useful alternative to thymol blue developed in the 1920s. Xylenol Blue can be used at half the concentration that thymol blue requires. It is functional at the same two pH ranges, acidic (pH 1-3), which transitions from red to yellow, and basic (pH 8-10), which transitions from yellow to blue. Xylenol Blue may be substituted for any experiment that requires thymol blue.

Product Specifications

Catalog ID X-040
CAS # 125-31-5
MW 410.49 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at room temperature.
View
Packs & Pricing
,
X-040-50 2-3 Weeks 50 g $ 356.00
X-040-100 2-3 Weeks 100 g $ 622.00
X-040-250 2-3 Weeks 250 g $ 1,288.00
X-040-500 2-3 Weeks 500 g $ 2,131.00

Y-β-D-GLC

Catalog ID Size Pricing
I-470-250MG 250 mg $ 93.00
I-470-5 5 g $ 867.00

Description

Y-β-D-GLC is a chromogenic substrate for β-D-glucosidase, yielding a blue precipitate upon cleavage.

Product Specifications

Catalog ID I-470
CAS # 487-60-5
MW 295.29 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Packs & Pricing
,
I-470-250MG 2-3 Weeks 250 mg $ 93.00
I-470-5 2-3 Weeks 5 g $ 867.00

Yeast Lysis Buffer Kit plus Zymolyase

Catalog ID Size Pricing
GB-178 100 Preps $ 199.00

Description

The Yeast Lysis Buffer Kit is useful for extraction of soluble proteins from yeast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

This kit is provided with an optional protocol to make spheroplast and remove lytic enzyme Zymolyase®, prior to lysis and extraction of yeast proteins. Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer.

The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works with both gram-positive and gram-negative bacteria. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.

This kit is suitable for processing 100 x 50 µl yeast cell pellet preparations. Yeast Lysis Buffer is also available separately for further downstream processing.

Product Specifications

Catalog ID GB-178
Storage/Handling Store at -20°C.
View
Packs & Pricing
,
GB-178 2-3 Weeks 100 Preps $ 199.00

Yeast Spheroplast Lysis Buffer

Catalog ID Size Pricing
GB-179-100 100 mL $ 75.00
GB-179-500 500 mL $ 145.00

Description

The Yeast Spheroplast Lysis Buffer is useful for extraction of soluble proteins from spheroplast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer. The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works well with both gram-positive and gram-negative bacteria.

The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.

Product Specifications

Catalog ID GB-179
Storage/Handling Store at -20°C.
View
Packs & Pricing
,
GB-179-100 In stock 100 mL $ 75.00
GB-179-500 In stock 500 mL $ 145.00

Join our list to receive promos and articles.

Join Now