Biological Buffers

Biological buffers are pH stabilized solutions used for performing electrophoresis, blotting tests, or analyzing cell cultures, and they all have the benefit of being water soluble. Most molecules suitable as buffers can be grouped into one of six different structural families, each with their own range of pH values. GoldBio supplies a wide range of buffers to function with your research condition and pH. Our contingent of Good’s buffers offer both stability and a minimum impact to your biological system, ensuring that you have the best buffer to suit your research needs!
Showing 1-20 of 26 items.

ACES

Catalog ID Size Pricing
A-010-25 25 g $ 30.00
A-010-50 50 g $ 47.00
A-010-100 100 g $ 58.00

Description

ACES is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is an acetamido buffer that is useful for a pH range of 6.1 – 7.5. ACES is used as buffering component in cell culture media, in both agarose and polyacrylamide electrophoresis, and in isoelectric focusing of proteins. ACES forms a complex with most common metals and formation constants should be taken into account when using this buffer in a solution containing metal ions. ACES absorbs UV light at 230 nm.

Product Specifications

Catalog ID A-010
CAS # 7365-82-4
MW 182.20 g/mol
Grade HIGH PURITY GRADE
Storage/Handling Store at room temperature.
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A-010-25 25 g $ 30.00
A-010-50 50 g $ 47.00
A-010-100 100 g $ 58.00

ADA

Catalog ID Size Pricing
A-780-25 25 g $ 24.00
A-780-50 50 g $ 29.00
A-780-100 100 g $ 41.00

Description

ADA is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is an acetamido buffer that is useful for a pH range of 6.0 – 7.2. ADA forms a complex with most common metals and stability constants should be taken into account when using this buffer. It has been used as a complexing agent to effectively help remove contaminant metals from soil. ADA absorbs UV light between 0.1 and 260 nm. Solubility of ADA increases if the free acid is converted to the sodium salt with NaOH.

Product Specifications

Catalog ID A-780
CAS # 26239-55-4
MW 190.22 g/mol
Grade ULTRA PURE GRADE
Storage/Handling Store at room temperature.
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A-780-25 25 g $ 24.00
A-780-50 50 g $ 29.00
A-780-100 100 g $ 41.00

BES

Catalog ID Size Pricing
B-780-25 25 g $ 22.00
B-780-50 50 g $ 32.00
B-780-100 100 g $ 45.00

Description

BES is a zwitterionic buffering agent used in biochemistry and molecular biology that was first selected and described by Good et al. It is a bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 6.4 – 7.8. It is soluble in water at 0°C up to 3.2M. Like most neutral, amine-based buffers, BES forms a complex with DNA. It interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in BES shields the negative charge of DNA to a lesser extent than less substituted buffers. BES can be used in a buffered saline solution in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. BES also forms a complex with certain metals, including copper and cobalt. Though BES binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.

Product Specifications

Catalog ID B-780
CAS # 10191-18-1
MW 213.25 g/mol
Grade ULTRA PURE GRADE
Storage/Handling Store at room temperature.
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B-780-25 25 g $ 22.00
B-780-50 50 g $ 32.00
B-780-100 100 g $ 45.00

Bicine

Catalog ID Size Pricing
B-785-25 25 g $ 21.00
B-785-50 50 g $ 27.00
B-785-100 100 g $ 35.00
B-785-500 500 g $ 126.00
B-785-1 1 kg $ 228.00

Description

Bicine is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, bis(2-hydroxyethyl)amine buffer that is useful for a pH range of 7.6 – 9.0. It is commonly used in protein crystallization, in the study of enzymatic reactions, and for electrophoresis. It is used in thin layer ion exchange chromatography for protein resolution, as well as a multiphasic buffer system for SDS-PAGE of proteins. It is soluble in water at 0°C up to 1.1 M. Bicine forms a complex with most common metals so stability constants and concentrations should be taken into consideration when choosing this buffer.

Product Specifications

Catalog ID B-785
CAS # 150-25-4
MW 163.17 g/mol
Grade ULTRA PURE GRADE
Storage/Handling Store at room temperature.
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B-785-25 25 g $ 21.00
B-785-50 50 g $ 27.00
B-785-100 100 g $ 35.00
B-785-500 500 g $ 126.00
B-785-1 1 kg $ 228.00

Bis-Tris

Catalog ID Size Pricing
B-020-100 100 g $ 53.00
B-020-250 250 g $ 91.00
B-020-500 500 g $ 138.00
B-020-1 1 kg $ 235.00

Description

Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.

Product Specifications

Catalog ID B-020
CAS # 6976-37-0
MW 209.24 g/mol
Storage/Handling Store at room temperature.
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B-020-100 100 g $ 53.00
B-020-250 250 g $ 91.00
B-020-500 500 g $ 138.00
B-020-1 1 kg $ 235.00

CAPS

Catalog ID Size Pricing
C-040-25 25 g $ 22.00
C-040-50 50 g $ 32.00
C-040-100 100 g $ 45.00
C-040-500 500 g $ 108.00

Description

CAPS is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a cyclohexylamine buffer that is useful for a pH range of 9.7 – 11.1 and commonly used to study enzymatic processes above physiological pH. CAPS is an excellent buffer for the transfer of high molecular weight proteins to nitrocellulose membranes after SDS-PAGE. It is also used in protein sequencing. CAPS displays only weak or negligible complexation capabilities for some metals making it a suitable non-coordinating buffer for use in solutions containing metal ions. CAPS is suitable for use with the bicinchoninic acid (BCA) assay.

Product Specifications

Catalog ID C-040
CAS # 1135-40-6
MW 221.32 g/mol
Storage/Handling Store desiccated at room temperature.
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C-040-25 25 g $ 22.00
C-040-50 50 g $ 32.00
C-040-100 100 g $ 45.00
C-040-500 500 g $ 108.00

CHES

Catalog ID Size Pricing
C-870-25 25 g $ 25.00
C-870-50 50 g $ 29.00
C-870-100 100 g $ 43.00
C-870-500 500 g $ 164.00

Description

CHES is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a cyclohexylamine buffer that is useful for a pH range of 8.6 – 10.0 and commonly used to study enzymatic processes above physiological pH. CHES displays only weak or negligible complexation capabilities for some metals making it a suitable non-coordinating buffer for use in most solutions containing metal ions. CHES displays high affinity for the iodoacetate binding site of liver alcohol dehydrogenase, protecting the enzyme from inactivation by iodoacetic acid.

Product Specifications

Catalog ID C-870
CAS # 103-47-9
MW 207.29 g/mol
Grade ULTRA PURITY GRADE
Storage/Handling Store at room temperature.
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C-870-25 25 g $ 25.00
C-870-50 50 g $ 29.00
C-870-100 100 g $ 43.00
C-870-500 500 g $ 164.00

HEPES, Free Acid

Catalog ID Size Pricing
H-400-100 100 g $ 37.00
H-400-250 250 g $ 74.00
H-400-500 500 g $ 112.00
H-400-1 1 kg $ 175.00
H-400-2 2 x 1 kg $ 290.00
H-400-5 5 kg $ 612.00

Description

HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

Product Specifications

Catalog ID H-400
CAS # 7365-45-9
MW 238.30 g/mol
Grade ULTRA PURE GRADE
Storage/Handling Store at room temperature.
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H-400-100 100 g $ 37.00
H-400-250 250 g $ 74.00
H-400-500 500 g $ 112.00
H-400-1 1 kg $ 175.00
H-400-2 2 x 1 kg $ 290.00
H-400-5 5 kg $ 612.00

HEPES, Sodium Salt

Catalog ID Size Pricing
H-401-50 50 g $ 34.00
H-401-100 100 g $ 58.00
H-401-500 500 g $ 169.00
H-401-1 1 kg $ 281.00
H-401-2.5 2.5 kg $ 622.00
H-401-5 5 kg $ 1,080.00

Description

HEPES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It is commonly used in cell culture media and as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPES interferes with reactions between DNA and restriction enzymes to a lesser extent than similar buffers with less substituted amine groups, such as Tris. This lack of interference occurs because steric hindrance of the protonated tertiary amine in HEPES shields the negative charge of DNA to a lesser extent than less substituted buffers. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.

Product Specifications

Catalog ID H-401
CAS # 75277-39-3
MW 260.29 g/mol
Grade HIGH PURITY GRADE
Storage/Handling Store desiccated at room temperature.
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H-401-50 50 g $ 34.00
H-401-100 100 g $ 58.00
H-401-500 500 g $ 169.00
H-401-1 1 kg $ 281.00
H-401-2.5 2.5 kg $ 622.00
H-401-5 5 kg $ 1,080.00

HEPPSO

Catalog ID Size Pricing
H-480-25 25 g $ 44.00
H-480-50 50 g $ 60.00
H-480-100 100 g $ 82.00

Description

HEPPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 7.1 – 8.5. It is commonly used as an ampholytic separator to create a pH gradient in isoelectric focusing. HEPPSO displays no toxicity for crustaceans with which it has been studied and has been deemed suitable for toxicological studies. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. HEPPSO forms a complex with Cu(II) ions so stability constants and concentrations should be taken into consideration when using this buffer in a solution containing copper ions. Though HEPPSO binds to coppers ions, it is still suitable for use with the bicinchoninic acid (BCA) assay. Diminished protein-induced color development can be accounted for or even eliminated by precipitating protein prior to reaction with bicinchoninic acid.

Product Specifications

Catalog ID H-480
CAS # 68399-78-0
MW 268.33 g/mol
Grade ULTRA PURITY GRADE
Storage/Handling Store at room temperature.
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H-480-25 25 g $ 44.00
H-480-50 50 g $ 60.00
H-480-100 100 g $ 82.00

MES Free Acid Monohydrate, Ultra Pure

Catalog ID Size Pricing
M-090-100 100 g $ 60.00
M-090-250 250 g $ 91.00
M-090-500 500 g $ 132.00
M-090-1 1 kg $ 224.00

Description

MES monohydrate is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

Product Specifications

Catalog ID M-090
CAS # 145224-94-8
MW 213.25 g/mol
Storage/Handling Store desiccated at room temperature.
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M-090-100 100 g $ 60.00
M-090-250 250 g $ 91.00
M-090-500 500 g $ 132.00
M-090-1 1 kg $ 224.00

MES, Free Acid

Catalog ID Size Pricing
M-095-25 25 g $ 19.00
M-095-50 50 g $ 29.00
M-095-100 100 g $ 41.00
M-095-250 250 g $ 78.00
M-095-1 1 kg $ 263.00

Description

MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.

Product Specifications

Catalog ID M-095
CAS # 4432-31-9
MW 195.24 g/mol
Grade ULTRA PURITY GRADE
Storage/Handling Store at room temperature.
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M-095-25 25 g $ 19.00
M-095-50 50 g $ 29.00
M-095-100 100 g $ 41.00
M-095-250 250 g $ 78.00
M-095-1 1 kg $ 263.00

MES, Sodium Salt

Catalog ID Size Pricing
M-091-25 25 g $ 19.00
M-091-50 50 g $ 31.00
M-091-100 100 g $ 49.00
M-091-500 500 g $ 118.00
M-091-1 1 kg $ 188.00

Description

MES sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 – 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MES is often used in buffered culture media for bacteria, yeast, and mammalian cells. It is toxic towards plants at high concentrations, but can be used in culture media at lower concentrations for plants. Due to low ionic mobility, MES is regarded as an excellent buffer for use in capillary electrochromatography. MES is often employed as a substitute for the highly toxic cacodylate, as well as a substitute for the non-zwitterionic buffers citrate and malate. MES complexes with some organotin(IV) molecules that are being explored as antitumor agents.

Product Specifications

Catalog ID M-091
CAS # 71119-23-8
MW 217.22 g/mol
Storage/Handling Store desiccated at room temperature.
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M-091-25 25 g $ 19.00
M-091-50 50 g $ 31.00
M-091-100 100 g $ 49.00
M-091-500 500 g $ 118.00
M-091-1 1 kg $ 188.00

MOPS Free Acid, Ultra Pure

Catalog ID Size Pricing
M-790-100 100 g $ 35.00
M-790-250 250 g $ 60.00
M-790-500 500 g $ 95.00
M-790-1 1 kg $ 153.00
M-790-2 2 x 1 kg $ 273.00
M-790-5 5 kg $ 564.00

Description

MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

Product Specifications

Catalog ID M-790
CAS # 1132-61-2
MW 209.26 g/mol
Storage/Handling Store desiccated at room temperature.
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M-790-100 100 g $ 35.00
M-790-250 250 g $ 60.00
M-790-500 500 g $ 95.00
M-790-1 1 kg $ 153.00
M-790-2 2 x 1 kg $ 273.00
M-790-5 5 kg $ 564.00

MOPS, Sodium Salt

Catalog ID Size Pricing
M-791-25 25 g $ 20.00
M-791-100 100 g $ 55.00
M-791-250 250 g $ 87.00
M-791-500 500 g $ 120.00
M-791-1 1 kg $ 191.00

Description

MOPS sodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer that is useful for a pH range of 6.5 – 7.9 and commonly used for cell culture media, as a running buffer in electrophoresis and for protein purification in chromatography. MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. MOPS is often used in buffered culture media for bacteria, yeast, and mammalian cells. MOPS is regarded as an excellent buffer for use in separating RNA in agarose gels. It is recommended to sterilize MOPS buffers by filtration rather than with autoclave due to the unknown identity of yellow degradation products that occur after sterilization of MOPS with autoclave. It is suitable for use in the bicinchoninic acid (BCA) assay.

MOPS sodium salt can be mixed with MOPS free acid to attain desired pH. Alternatively, MOPS free acid can be titrated with sodium hydroxide to attain desired pH.

Product Specifications

Catalog ID M-791
CAS # 71119-22-7
MW 231.25 g/mol
Grade HIGH PURITY GRADE
Storage/Handling Store desiccated at room temperature.
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M-791-25 25 g $ 20.00
M-791-100 100 g $ 55.00
M-791-250 250 g $ 87.00
M-791-500 500 g $ 120.00
M-791-1 1 kg $ 191.00

MOPSO, Free Acid

Catalog ID Size Pricing
M-795-25 25 g $ 22.00
M-795-50 50 g $ 32.00
M-795-100 100 g $ 45.00

Description

MOPSO is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, morpholinic buffer, structurally similar to MOPS, that is useful for a pH range of 6.2 – 7.6. It is commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification with chromatography. Due to low ionic mobility, MOPSO is regarded as an excellent buffer for use in capillary electrochromatography. MOPSO lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It does form a complex with some organotin(IV) molecules that are being explored as antitumor agents. MOPSO also interacts with the peptide backbone of bovine serum albumin (BSA) to stabilize BSA against thermal denaturation.

Product Specifications

Catalog ID M-795
CAS # 68399-77-9
MW 225.26 g/mol
Grade ULTRA PURITY GRADE
Storage/Handling Store desiccated at -20°C.
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M-795-25 25 g $ 22.00
M-795-50 50 g $ 32.00
M-795-100 100 g $ 45.00

MOPSO, Sodium Salt

Catalog ID Size Pricing
M-799-25 25 g $ 28.00
M-799-50 50 g $ 44.00
M-799-100 100 g $ 55.00

Description

MOPSO sodium salt is a zwitterionic buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a morpholinic buffer, structurally similar to MOPS, that is useful for a pH range of 6.2 – 7.6. It is commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification with chromatography. Due to low ionic mobility, MOPSO is regarded as an excellent buffer for use in capillary electrochromatography. MOPSO lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. It does form a complex with some organotin(IV) molecules that are being explored as antitumor agents. MOPSO also interacts with the peptide backbone of bovine serum albumin (BSA) to stabilize BSA against thermal denaturation.

Product Specifications

Catalog ID M-799
CAS # 79803-73-9
MW 247.24 g/mol
Grade ULTRA PURITY GRADE
Storage/Handling Store at room temperature.
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M-799-25 25 g $ 28.00
M-799-50 50 g $ 44.00
M-799-100 100 g $ 55.00

PBS (Phosphate Buffered Saline) Tablets

There is no image for PBS (Phosphate Buffered Saline) Tablets PBS (Phosphate Buffered Saline) Tablets
Catalog ID Size Pricing
P-271-50 50 tablets $ 49.00
P-271-200 200 tablets $ 90.00

Description

PBS (phosphate buffered saline) is a common buffer solution used in the laboratory. It is an isotonic and non-toxic buffer that is meant to mimic the osmolarity and ion concentrations of the human body. PBS is used for immunoassays, for sample dilution, for protein purification, and as a wash buffer in cell cultures. PBS tablets are premeasured tablets for convenient preparation of 1X PBS solution at a pH of 7.3 – 7.5. PBS buffer contains 10 mM phosphate buffer, 137 mM sodium chloride, and 2.7 mM potassium chloride.

Product Specifications

Catalog ID P-271
Grade BIOTECHNOLOGY GRADE
Storage/Handling Store desiccated at room temperature.
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P-271-50 50 tablets $ 49.00
P-271-200 200 tablets $ 90.00

PIPES, Free Acid

Catalog ID Size Pricing
P-281-100 100 g $ 37.00
P-281-250 250 g $ 57.00
P-281-500 500 g $ 103.00
P-281-1 1 kg $ 176.00
P-281-2 2 x 1 kg $ 285.00

Description

PIPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1 – 7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography.

Product Specifications

Catalog ID P-281
CAS # 5625-37-6
MW 302.37 g/mol
Grade HIGH PURITY GRADE
Storage/Handling Store desiccated at room temperature.
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P-281-100 100 g $ 37.00
P-281-250 250 g $ 57.00
P-281-500 500 g $ 103.00
P-281-1 1 kg $ 176.00
P-281-2 2 x 1 kg $ 285.00

PIPES, Sodium Salt

Catalog ID Size Pricing
P-280-100 100 g $ 37.00
P-280-250 250 g $ 57.00
P-280-500 500 g $ 103.00

Description

PIPES sesquisodium salt is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.1 – 7.5. PIPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions. PIPES has wide variety of applications and is commonly used in cell culture media, in protein crystallization, as a running buffer in gel electrophoresis, and as an eluent in isoelectric focusing and chromatography. This buffer is capable of forming radicals and is therefore not suitable for redox reactions. It is suitable for use in the bicinchoninic acid (BCA) assay.

Product Specifications

Catalog ID P-280
CAS # 100037-69-2
MW 335.34 g/mol
Grade HIGH PURITY GRADE
Storage/Handling Store desiccated at room temperature.
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P-280-100 100 g $ 37.00
P-280-250 250 g $ 57.00
P-280-500 500 g $ 103.00

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