Buffers & Washes for Chromatography

Buffers and washes are important for purification of proteins in a chromatography system. In chromatography, the primary buffers help the proteins bind with ligands present in the matrix. To purify this sample an elution buffer, or wash, is used to separate out unwanted proteins. It is important for both types of buffers to be of high quality to ensure the maximum purity of your sample, and GoldBio provides a variety of buffers and washes at affordable prices.
Showing 1-5 of 5 items.

5X Phosphate Buffer + 3M Imidazole Solution

Catalog ID Size Pricing
I-905-100 100 mL $ 184.00

Description

This Buffer Set consists of:

- 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)
- 25 mL 3M Recrystallized Imidazole Solution, pH 6.0

This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.

Product Specifications

Catalog ID I-905
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I-905-100 100 mL $ 184.00

5X Phosphate Buffer, pH 8

Catalog ID Size Pricing
P-500-50 50 mL $ 61.00
P-500-100 100 mL $ 86.00
P-500-250 250 mL $ 121.00

Description

5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).

Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.

Product Specifications

Catalog ID P-500
Storage/Handling Store at 4°C.
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P-500-50 50 mL $ 61.00
P-500-100 100 mL $ 86.00
P-500-250 250 mL $ 121.00

His-Tag Buffer Set

Catalog ID Size Pricing
I-906-250 1 kit $ 118.00

Description

His-Tag Buffer Set includes:

- 250 mL Wash Buffer (50mM Phosphate Buffer pH 8, 300mM NaCl, 10mM Imidazole)
- 50 mL Elution Buffer (50mM Phosphate Buffer, 300mM NaCl, 500mM Imidazole)

Designed for use with Goldbio Nickel and Cobalt Agarose resins, but it can be used with most standard His-tag purification resins.

Product Specifications

Catalog ID I-906
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I-906-250 1 kit $ 118.00

Imidazole - 3M Recrystallized Solution, pH 6.0

Catalog ID Size Pricing
I-901-25 25 mL $ 72.00
I-901-50 50 mL $ 107.00
I-901-100 100 mL $ 164.00
I-901-250 250 mL $ 380.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-901
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at 4°C.
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I-901-25 25 mL $ 72.00
I-901-50 50 mL $ 107.00
I-901-100 100 mL $ 164.00
I-901-250 250 mL $ 380.00

Imidazole, Recrystallized

Catalog ID Size Pricing
I-902-25 25 g $ 61.00
I-902-50 50 g $ 103.00
I-902-100 100 g $ 165.00
I-902-500 500 g $ 505.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-902
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at room temperature.
UN Number 3262
Item Class 8
Group Number III
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I-902-25 25 g $ 61.00
I-902-50 50 g $ 103.00
I-902-100 100 g $ 165.00
I-902-500 500 g $ 505.00

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