Buffers and Washes

GoldBio offers various buffers for your affinity purification demands. We provide basic washing and elution buffers, already prepared and ready to use, as well as a His-tag buffer set which provides all the buffers necessary for purification. GoldBio’s recrystallized imidazole, used for eluting His-tagged proteins in protein purification, has the highest purity available anywhere on the market and is available in a prepared, ready solution or in dry form to create your lab preferred concentrations.
Showing 1-5 of 5 items.

5X Phosphate Buffer + 3M Imidazole Solution

Catalog ID Size Pricing
I-905-100 100 mL $ 201.00

Description

This Buffer Set consists of:

- 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)
- 25 mL 3M Recrystallized Imidazole Solution, pH 6.0

This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.

Product Specifications

Catalog ID I-905
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I-905-100 100 mL $ 201.00

5X Phosphate Buffer, pH 8

Catalog ID Size Pricing
P-500-50 50 mL $ 67.00
P-500-100 100 mL $ 95.00
P-500-250 250 mL $ 133.00

Description

5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).

Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.

Product Specifications

Catalog ID P-500
Storage/Handling Store at 4°C.
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P-500-50 50 mL $ 67.00
P-500-100 100 mL $ 95.00
P-500-250 250 mL $ 133.00

His-Tag Buffer Set

Catalog ID Size Pricing
I-906-250 1 kit $ 129.00

Description

His-Tag Buffer Set includes:

- 250 mL Wash Buffer (50mM Phosphate Buffer pH 8, 300mM NaCl, 10mM Imidazole)
- 50 mL Elution Buffer (50mM Phosphate Buffer, 300mM NaCl, 500mM Imidazole)

Designed for use with Goldbio Nickel and Cobalt Agarose resins, but it can be used with most standard His-tag purification resins.

Product Specifications

Catalog ID I-906
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I-906-250 1 kit $ 129.00

Imidazole - 3M Recrystallized Solution, pH 6.0

Catalog ID Size Pricing
I-901-25 25 mL $ 79.00
I-901-50 50 mL $ 117.00
I-901-100 100 mL $ 180.00
I-901-250 250 mL $ 414.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-901
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at 4°C.
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I-901-25 25 mL $ 79.00
I-901-50 50 mL $ 117.00
I-901-100 100 mL $ 180.00
I-901-250 250 mL $ 414.00

Imidazole, Recrystallized

Catalog ID Size Pricing
I-902-25 25 g $ 67.00
I-902-50 50 g $ 113.00
I-902-100 100 g $ 181.00
I-902-500 500 g $ 550.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-902
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at room temperature.
UN Number 3262
Item Class 8
Group Number III
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I-902-25 25 g $ 67.00
I-902-50 50 g $ 113.00
I-902-100 100 g $ 181.00
I-902-500 500 g $ 550.00

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