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GoldBio’s new ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail is specifically formulated for the most demanding protein purification applications. The optimized concentrations of the various inhibitors in our Extra Strength cocktail deliver an enhanced dose of protease inhibition for the toughest research experiments. ProBlock™ Gold Extra Strength contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases.

ProBlock™ Gold mammalian protease inhibitor cocktail contains optimized concentrations of various mammalian protease inhibitors, which provide excellent inhibition of protease activities during protein purification from mammalian cells and tissues. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine and specific inhibitors for mammalian proteases, such as aminopeptidases. A 1X concentration of ProBlock™ Gold Mammalian inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca²⁺, Mg²⁺ or Mn²⁺) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Mammalian is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

ProBlock™ Gold Plant protease inhibitor cocktail contains optimized concentrations of various plant protease inhibitors, which provide excellent inhibition of protease activities during protein purification from plant. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for plant proteases, such as aspartic proteases and aminopeptidases. A 1X concentration oft ProBlock™ Gold Plant inhibits over 90% of protease activities.

Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during purification from animal tissues, plant tissues, yeast and bacteria. ProBlock™ Gold contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. An separate bottle of EDTA is provided to inhibit metalloproteases.

Since some proteins require divalent cations like Ca²⁺, Mg²⁺ or Mn²⁺ for their biological activity, the presence of EDTA may be detrimental to the protein activity. Furthermore, if the protein of interest is purified using immobilized metal chelate affinity chromatography, EDTA must be removed from the buffer before the chromatography. The ProBlock™ Gold is therefore supplied with a separate, optional EDTA solution which may be added to the extraction buffer or lysate as needed.

ProBlock™ Gold Yeast/Fungi protease inhibitor cocktail contains optimized concentrations of various yeast and fungal protease inhibitors, which provide excellent inhibition of protease activities during protein purification from yeast or fungi. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for yeast or fungal proteases, such as aspartic proteases. At 1X concentration, ProBlock™ Gold Yeast/Fungi inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca²⁺, Mg²⁺ or Mn²⁺) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Yeast/Fungi is supplied with additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

ProBlock™-100, EDTA Free is designed for large volumes of extraction/lysis buffers and is supplied as 10 vials per kit, with each vial suitable for 100 ml.

ProBlock-100, EDTA Free protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail is EDTA free and contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

ProBlock™-100, Plus EDTA is designed for large volumes of extraction/ lysis buffers and is supplied as 10 vials /kit, with each vial suitable for 100 ml.

ProBlock™-100, Plus EDTA protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is packed separately for inhibiting metalloproteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

ProBlock™-50, EDTA Free is designed for large volumes of extraction/lysis buffers and is supplied as 1 vial/kit (Cat# GB-326-1), 10 vials /kit (Cat# GB-326-10) or 20 vials/kit (Cat# GB-326-20), with each vial suitable for 50 ml.

ProBlock-50, EDTA Free protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail is EDTA free and contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

ProBlock™-50, Plus EDTA is designed for large volumes of extraction/lysis buffers and is supplied as 10 vials /kit (Cat# GB-334-10) or 20 vials/kit (Cat# GB-334-20), with each vial suitable for 50 ml sample. EDTA [0.5M, 100X] is also supplied with this kit as an optional metalloprotease inhibitor.

ProBlock™-50, Plus EDTA protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is packed separately for inhibiting metalloproteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

GoldBio’s RR1 chemically competent E. coli cells are suitable for high-efficiency transformation in a wide variety of applications such as cloning and sub-cloning. E. coli RR1 is a recA+ derivative of the HB101 strain and can be more useful than HB101 if a recA+ background is required.

Product Specifications
Competent cell type: Chemically competent
Derivative of: RR1
Species: E. coli
Format: Tubes
Transformation efficiency: ≥3 x 10⁷ cfu/µg pUC19 DNA
Blue/white screening: No

Simple Stop™ 1 is a broad-spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors in two solutions that target all the phosphatase categories: serine/threonine (Ser/Thr) specific phosphatase, tyrosine specific phosphatase, dual specificity phosphatases, acid phosphatase and alkaline phosphatase.

Each Simple Stop™ phosphatase inhibitor cocktail solution is a 100X concentrated, ready-to-use, solution. Simply add cocktail solution “A” and cocktail solution “B” to your extraction buffers or samples and you are ready to go. This cocktail is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most common protein assays.

Simple Stop™ 2 is a broad-spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors in two solutions that target all the phosphatase categories: serine/threonine (Ser/Thr) specific phosphatase, tyrosine specific phosphatase, dual specificity phosphatases, acid phosphatase and alkaline phosphatase.

Each Simple Stop™ phosphatase inhibitor cocktail solution is a 100X concentrated, ready-to-use, solution. Simply add cocktail solution “A” and cocktail solution “B” to your extraction buffers or samples and you are ready to go. This cocktail is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most common protein assays.

Simple Stop^TM 3 is a phosphatase inhibitor cocktail consisting of three phosphatase inhibitors that target alkaline and serine/threonine phosphatases. It is a stabilized solution of cantharidin, bromotetramisole and microcystin LR. It is compatible with IEF/2D studies.

The Simple Stop^TM phosphatase inhibitor cocktails are 100X concentrated, ready-to-use solutions that are simply added to your extraction buffers or samples. They are ideal for inhibition in tissue extractions and cell lysis experiments and are compatible with most common protein assays.

GoldBio’s TG1 Phage Display Electrocompetent E. coli cells are suitable for protein expression and preparation of antibody or peptide phage display libraries.TG1 Phage Display cells have ≥1 x 10⁹ cfu/µg efficiency with electroporation and have Amber suppressor strain (supE).

Product Specifications
Competent cell type: ElectroCompetent
Species: E. coli
Format: Tubes
Transformation efficiency: ≥1 x 10⁹ cfu/µg pUC19 DNA
Blue/white screening: Yes

Timentin™ is a 15:1 mixture of ticarcillin and clavulanate. Ticarcillin is a penicillin ß-lactam antibiotic, which is susceptible to ß-lactamase degradation. When combined with clavulanate, a ß-lactamase inhibitor, its efficacy is greatly increased. Timentin™ exhibits high activity against gram-negative bacteria and resistant Agrobacterium species.

Timentin™ is a 15:1 mixture of ticarcillin and clavulanate. Ticarcillin is a penicillin ß-lactam antibiotic, which is susceptible to ß-lactamase degradation. When combined with clavulanate, a ß-lactamase inhibitor, its efficacy is greatly increased. Timentin™ exhibits high activity against gram-negative bacteria and resistant Agrobacterium species.

The Timentin™ EZ Pak™ is the fastest and easiest way to make a set amount of sterile Timentin™ solution. The kit includes pre weighed Timentin™ powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H₂O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio Timentin™ and the sterile solution is ready for tissue culture, bacterial media, or any number of uses.

Tissue Cell Lysis Buffer has been developed for extraction of total soluble protein from fresh or frozen animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue Cell Lysis Buffer. Tissue Cell Lysis Buffer reagent has been tested for use with a wide variety of animal tissues.

α-Chloralose is used as an anesthetic and analgesia for laboratory animals.

α-Cyclodextrin is a polysaccharide of six glucose units that are covalently attached end-to-end via α-1, 4 linkages. There are two other naturally occurring cyclodextrins, β- and Ɣ-, which contain seven and eight glucose units respectively. In water, these fibers take on a toroid or truncated cone configuration. In aqueous medium, the exterior surface of cyclodextrins is hydrophilic while the interior core is hydrophobic. This allows for the formation of guest-host relationships with small hydrophobic molecules.

α-Cyclodextrin is a natural, soluble dietary fiber. It has the unique ability among known fibers to bind nine times its weight in fat. This high ratio of binding makes α-Cyclodextrin practical as a weight loss supplement.