Cell Lysis

Many downstream applications require cell membranes to be broken up. Cell lysis forms the fundamental first step in the analysis of DNA and RNA extraction, and protein purification. Proteins removed via lysis can later be analyzed via electrophoresis, blotting or assays. Lysozyme helps break down the bacterial cell wall, while DNases improve your protein recovery. To simplify your extraction and get consistent results, try GoldBio’s pre-made cell lysis buffers. GoldBio’s lysis reagents are designed to help you achieve maximum protein recovery without compromising your research budget.

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Showing 1-16 of 16 items.

Bacterial Cell Lysis Buffer

Catalog ID Size Pricing
GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-176-100 100 Preps $ 165.00
GB-176-250 250 Preps $ 289.00
GB-176-500 500 Preps $ 533.00

Description

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Product Specifications

Catalog ID GB-177
Storage/Handling Store at 4°C. Do NOT freeze.
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GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-176-100 100 Preps $ 165.00
GB-176-250 250 Preps $ 289.00
GB-176-500 500 Preps $ 533.00

Cellulase R-10

There is no image for Cellulase R-10 Cellulase R-10
Catalog ID Size Pricing
C8001.0001 1 g $ 83.00
C8001.0005 5 g $ 162.00
C8001.0010 10 g $ 253.00

Description

Cellulase R-10, also called Cellulase "Onozuka" R-10, is derived from Trichoderma Viride. Cellulase R-10 is a multi-enzymatic system consisting of various enzymes, including cellulase, hemicellulase, pectinase and protease. Cellulase R-10 is typically used in association with macerozyme R-10 in the decomposition of plant cell walls to produce protoplasts.

Product Specifications

Catalog ID C8001
CAS # 9012-54-8
Storage/Handling Store at room temperature.
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C8001.0001 1 g $ 83.00
C8001.0005 5 g $ 162.00
C8001.0010 10 g $ 253.00

Cellulase RS

There is no image for Cellulase RS Cellulase RS
Catalog ID Size Pricing
C8003.0001 1 g $ 128.00
C8003.0005 5 g $ 351.00
C8003.0010 10 g $ 552.00

Description

Cellulase “Onozuka RS” is produced by a mutant Trichoderma viride that was derived from the parent strain for Cellulase “Onozuka -R-10”. Cellulase RS contains a very high activity of decomposing natural celluloses. This type of cellulase can be used to obtain protoplasts in a very short time and dissolves cell walls of a wider range of plants.

Product Specifications

Catalog ID C8003
CAS # 9012-54-8
Storage/Handling Store desiccated at 4°C.
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C8003.0001 1 g $ 128.00
C8003.0005 5 g $ 351.00
C8003.0010 10 g $ 552.00

DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg
Catalog ID Size Pricing
D-301-50 50 mg $ 69.00
D-301-100 100 mg $ 101.00
D-301-500 500 mg $ 419.00
D-301-1 1 g $ 799.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca2+.

Product Specifications

Catalog ID D-301
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-301-50 50 mg $ 69.00
D-301-100 100 mg $ 101.00
D-301-500 500 mg $ 419.00
D-301-1 1 g $ 799.00

DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg
Catalog ID Size Pricing
D-303-100 100 mg $ 30.00
D-303-500 500 mg $ 89.00
D-303-1 1 g $ 161.00
D-303-5 5 g $ 657.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5mM Ca 2+.

Product Specifications

Name DNase I
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-303-100 100 mg $ 30.00
D-303-500 500 mg $ 89.00
D-303-1 1 g $ 161.00
D-303-5 5 g $ 657.00

DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg
Catalog ID Size Pricing
D-300-100 100 mg $ 37.00
D-300-500 500 mg $ 111.00
D-300-1 1 g $ 201.00
D-300-5 5 g $ 822.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.

Product Specifications

Catalog ID D-300
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-300-100 100 mg $ 37.00
D-300-500 500 mg $ 111.00
D-300-1 1 g $ 201.00
D-300-5 5 g $ 822.00

Elastase, Porcine Pancreas

There is no image for Elastase, Porcine Pancreas Elastase, Porcine Pancreas
Catalog ID Size Pricing
E-240-100 100 mg $ 45.00
E-240-500 500 mg $ 75.00
E-240-1 1 g $ 135.00
E-240-5 5 g $ 360.00

Description

Elastase is used for tissue dissociation and cell isolation applications. Elastase has an activity of >30 units/mg and is unstable at < pH 3.5. Elastase was purified from a porcine pancreas and then purified by affinity chromatography to remove trace amounts of contaminating proteases. One unit corresponds to the amount of enzyme which liberates 1 µmol 4-nitroaniline per minute at 25°C and pH 7.8.

Product Specifications

Catalog ID E-240
CAS # 39445-21-1
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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E-240-100 100 mg $ 45.00
E-240-500 500 mg $ 75.00
E-240-1 1 g $ 135.00
E-240-5 5 g $ 360.00

Guanidine HCl

Catalog ID Size Pricing
G-211-50 50 g $ 23.00
G-211-100 100 g $ 28.00
G-211-500 500 g $ 45.00
G-211-1 1 kg $ 66.00
G-211-3 3 kg $ 159.00
G-211-5 5 kg $ 218.00

Description

Guanidine HCl is a freely soluble chaotropic agent routinely used for protein denaturation and folding.

Product Specifications

Catalog ID G-211
CAS # 50-01-1
MW 95.53 g/mol
Storage/Handling Store at room temperature.
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G-211-50 50 g $ 23.00
G-211-100 100 g $ 28.00
G-211-500 500 g $ 45.00
G-211-1 1 kg $ 66.00
G-211-3 3 kg $ 159.00
G-211-5 5 kg $ 218.00

Lysozyme, Egg White

There is no image for Lysozyme, Egg White Lysozyme, Egg White
Catalog ID Size Pricing
L-040-1 1 g $ 28.00
L-040-5 5 g $ 44.00
L-040-10 10 g $ 61.00
L-040-25 25 g $ 109.00
L-040-100 100 g $ 218.00
L-040-250 250 g $ 502.00

Description

Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.

Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.

Product Specifications

Catalog ID L-040
CAS # 12650-88-3
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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L-040-1 1 g $ 28.00
L-040-5 5 g $ 44.00
L-040-10 10 g $ 61.00
L-040-25 25 g $ 109.00
L-040-100 100 g $ 218.00
L-040-250 250 g $ 502.00

Macerozyme R-10

There is no image for Macerozyme R-10 Macerozyme R-10
Catalog ID Size Pricing
M8002.0001 1 g $ 83.00
M8002.0005 5 g $ 162.00
M8002.0010 10 g $ 253.00

Description

Macerozyme R-10 is a macerating enzyme from the Rhizopus sp. which is perfectly suited for the isolation of plant cells. Macerozyme R-10 is often used in combination with Cellulase "Onozuka R-10" in the isolation of plant protoplasts.

Product Specifications

Catalog ID M8002
CAS # 9032-75-1
Storage/Handling Store at 4°C.
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M8002.0001 1 g $ 83.00
M8002.0005 5 g $ 162.00
M8002.0010 10 g $ 253.00

Mammalian Cell Lysis Buffer

Catalog ID Size Pricing
GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Description

Product Specifications

Catalog ID GB-180
Storage/Handling Store at 4°C.
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GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Pectolyase Y-23

Catalog ID Size Pricing
P8004.0001 1 g $ 367.00
P8004.0005 5 g $ 1,449.00

Description

No description available

Product Specifications

Catalog ID P8004
CAS # 9033-35-6
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P8004.0001 1 g $ 367.00
P8004.0005 5 g $ 1,449.00

Tissue Cell Lysis Buffer

Catalog ID Size Pricing
GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Description

Tissue Cell Lysis Buffer has been developed for extraction of total soluble protein from fresh or frozen animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue Cell Lysis Buffer. Tissue Cell Lysis Buffer reagent has been tested for use with a wide variety of animal tissues.

Product Specifications

Catalog ID GB-181
Storage/Handling Store at 4°C.
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GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Urea

Catalog ID Size Pricing
U-200-500 500 g $ 21.00
U-200-1 1 kg $ 31.00

Description

Urea is a nitrogenous organic compound composed of two amino groups and a carbonyl group and is commonly found as a metabolite in urine.Largely used as plant fertilizer, urea is metabolized by symbiotic urease producing soil bacteria into products readily assimilated by plants.In research, urea is commonly used in the denaturing of proteins for polyacrylamide gel electrophoresis as well as increasing the solubility of proteins in protein extraction and isolation by denaturing the insoluble inclusion bodies of recombinant proteins during production.

Product Specifications

Catalog ID U-200
CAS # 57-13-6
MW 60.06 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C.
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U-200-500 500 g $ 21.00
U-200-1 1 kg $ 31.00

Yeast Lysis Buffer Kit plus Zymolyase

Catalog ID Size Pricing
GB-178 100 Preps $ 171.00

Description

The Yeast Lysis Buffer Kit is useful for extraction of soluble proteins from yeast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

This kit is provided with an optional protocol to make spheroplast and remove lytic enzyme Zymolyase®, prior to lysis and extraction of yeast proteins. Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. A ready-to-use Zymolyase® preparation is also provided. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer.

The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works with both gram-positive and gram-negative bacteria. The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.

This kit is suitable for processing 100 x 50 µl yeast cell pellet preparations. Yeast Lysis Buffer is also available separately for further downstream processing.

Product Specifications

Catalog ID GB-178
Storage/Handling Store at -20°C.
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GB-178 100 Preps $ 171.00

Yeast Spheroplast Lysis Buffer

Catalog ID Size Pricing
GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

Description

The Yeast Spheroplast Lysis Buffer is useful for extraction of soluble proteins from spheroplast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer. The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works well with both gram-positive and gram-negative bacteria.

The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.

Product Specifications

Catalog ID GB-179
Storage/Handling Store at -20°C.
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GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

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