Cell Lysis

Many downstream applications require cell membranes to be broken up. Cell lysis forms the fundamental first step in the analysis of DNA and RNA extraction, and protein purification. Proteins removed via lysis can later be analyzed via electrophoresis, blotting or assays. Lysozyme helps break down the bacterial cell wall, while DNases improve your protein recovery. To simplify your extraction and get consistent results, try GoldBio’s pre-made cell lysis buffers. GoldBio’s lysis reagents are designed to help you achieve maximum protein recovery without compromising your research budget.

Sub-Categories

Showing 1-15 of 15 items.

Bacterial Cell Lysis Buffer

Catalog ID Size Pricing
GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-176-100 100 Preps $ 165.00
GB-176-250 250 Preps $ 289.00
GB-176-500 500 Preps $ 533.00

Description

No description available

Product Specifications

Catalog ID GB-177
Storage/Handling Store at 4°C. Do NOT freeze.
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GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-176-100 100 Preps $ 165.00
GB-176-250 250 Preps $ 289.00
GB-176-500 500 Preps $ 533.00

Cellulase R-10

There is no image for Cellulase R-10 Cellulase R-10
Catalog ID Size Pricing
C8001.0001 1 g $ 83.00
C8001.0005 5 g $ 162.00
C8001.0010 10 g $ 253.00

Description

No description available

Product Specifications

Catalog ID C8001
CAS # 9012-54-8
Storage/Handling Store at room temperature.
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C8001.0001 1 g $ 83.00
C8001.0005 5 g $ 162.00
C8001.0010 10 g $ 253.00

Cellulase RS

There is no image for Cellulase RS Cellulase RS
Catalog ID Size Pricing
C8003.0001 1 g $ 128.00
C8003.0005 5 g $ 351.00
C8003.0010 10 g $ 552.00

Description

Cellulase “Onozuka RS” is produced by a mutant Trichoderma viride that was derived from the parent strain for Cellulase “Onozuka -R-10”. Cellulase RS contains a very high activity of decomposing natural celluloses. This type of cellulase can be used to obtain protoplasts in a very short time and dissolves cell walls of a wider range of plants.

Product Specifications

Catalog ID C8003
CAS # 9012-54-8
Storage/Handling Store desiccated at 4°C.
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C8003.0001 1 g $ 128.00
C8003.0005 5 g $ 351.00
C8003.0010 10 g $ 552.00

DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg
Catalog ID Size Pricing
D-301-50 50 mg $ 69.00
D-301-100 100 mg $ 101.00
D-301-500 500 mg $ 419.00
D-301-1 1 g $ 799.00

Description

No description available

Product Specifications

Catalog ID D-301
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-301-50 50 mg $ 69.00
D-301-100 100 mg $ 101.00
D-301-500 500 mg $ 419.00
D-301-1 1 g $ 799.00

DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >300 Kunitz U/mg
Catalog ID Size Pricing
D-303-100 100 mg $ 30.00
D-303-500 500 mg $ 89.00
D-303-1 1 g $ 161.00
D-303-5 5 g $ 657.00

Description

No description available

Product Specifications

Name DNase I
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-303-100 100 mg $ 30.00
D-303-500 500 mg $ 89.00
D-303-1 1 g $ 161.00
D-303-5 5 g $ 657.00

DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg

There is no image for DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg DNase I, Bovine Pancreas (Lysate Tested), >500 Kunitz U/mg
Catalog ID Size Pricing
D-300-100 100 mg $ 37.00
D-300-500 500 mg $ 111.00
D-300-1 1 g $ 201.00
D-300-5 5 g $ 822.00

Description

DNase I (Deoxyribonuclease I) is a dialyzed lyophilized powder from bovine pancreas that has been purified by chromatography.

Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and phosphate group at the 5' position. The optimum pH is 7.8.

DNase I is activated by bivalent metals, and inhibited by chelating agents such as EDTA and SDS. DNase I can be stabilized against proteolytic digestion by 5 mM Ca2+.

Product Specifications

Catalog ID D-300
CAS # 9003-98-9
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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D-300-100 100 mg $ 37.00
D-300-500 500 mg $ 111.00
D-300-1 1 g $ 201.00
D-300-5 5 g $ 822.00

Elastase, Porcine Pancreas

There is no image for Elastase, Porcine Pancreas Elastase, Porcine Pancreas
Catalog ID Size Pricing
E-240-100 100 mg $ 45.00
E-240-500 500 mg $ 75.00
E-240-1 1 g $ 135.00
E-240-5 5 g $ 360.00

Description

No description available

Product Specifications

Catalog ID E-240
CAS # 39445-21-1
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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E-240-100 100 mg $ 45.00
E-240-500 500 mg $ 75.00
E-240-1 1 g $ 135.00
E-240-5 5 g $ 360.00

Guanidine HCl

Catalog ID Size Pricing
G-211-50 50 g $ 23.00
G-211-100 100 g $ 28.00
G-211-500 500 g $ 45.00
G-211-1 1 kg $ 66.00
G-211-3 3 kg $ 159.00
G-211-5 5 kg $ 218.00

Description

No description available

Product Specifications

Catalog ID G-211
CAS # 50-01-1
MW 95.53 g/mol
Storage/Handling Store at room temperature.
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G-211-50 50 g $ 23.00
G-211-100 100 g $ 28.00
G-211-500 500 g $ 45.00
G-211-1 1 kg $ 66.00
G-211-3 3 kg $ 159.00
G-211-5 5 kg $ 218.00

Lysozyme, Egg White

There is no image for Lysozyme, Egg White Lysozyme, Egg White
Catalog ID Size Pricing
L-040-1 1 g $ 28.00
L-040-5 5 g $ 44.00
L-040-10 10 g $ 61.00
L-040-25 25 g $ 109.00
L-040-100 100 g $ 218.00
L-040-250 250 g $ 502.00

Description

Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.

Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.

Product Specifications

Catalog ID L-040
CAS # 12650-88-3
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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L-040-1 1 g $ 28.00
L-040-5 5 g $ 44.00
L-040-10 10 g $ 61.00
L-040-25 25 g $ 109.00
L-040-100 100 g $ 218.00
L-040-250 250 g $ 502.00

Macerozyme R-10

There is no image for Macerozyme R-10 Macerozyme R-10
Catalog ID Size Pricing
M8002.0001 1 g $ 83.00
M8002.0005 5 g $ 162.00
M8002.0010 10 g $ 253.00

Description

No description available

Product Specifications

Catalog ID M8002
CAS # 9032-75-1
Storage/Handling Store at 4°C.
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M8002.0001 1 g $ 83.00
M8002.0005 5 g $ 162.00
M8002.0010 10 g $ 253.00

Mammalian Cell Lysis Buffer

Catalog ID Size Pricing
GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Description

Product Specifications

Catalog ID GB-180
Storage/Handling Store at 4°C.
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GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Tissue Cell Lysis Buffer

Catalog ID Size Pricing
GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Description

No description available

Product Specifications

Catalog ID GB-181
Storage/Handling Store at 4°C.
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GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Urea

Catalog ID Size Pricing
U-200-500 500 g $ 21.00
U-200-1 1 kg $ 31.00

Description

No description available

Product Specifications

Catalog ID U-200
CAS # 57-13-6
MW 60.06 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C.
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U-200-500 500 g $ 21.00
U-200-1 1 kg $ 31.00

Yeast Lysis Buffer Kit plus Zymolyase

Catalog ID Size Pricing
GB-178 100 Preps $ 171.00

Description

No description available

Product Specifications

Catalog ID GB-178
Storage/Handling Store at -20°C.
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GB-178 100 Preps $ 171.00

Yeast Spheroplast Lysis Buffer

Catalog ID Size Pricing
GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

Description

No description available

Product Specifications

Catalog ID GB-179
Storage/Handling Store at -20°C.
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GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

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