Cell Selection & Maintenance

As you go through the process of selecting and maintaining your cell lines for downstream research, GoldBio wants to provide you with the quality tools you need to effectively analyze, induce and isolate your protein samples. With hundreds of high quality antibiotics like ampicillin and hygromycin, the most affordable IPTG on the market, the essentials of electrophoretic reagents and our famously robust DNA ladders and agarose, GoldBio is determined to cover your research needs from selection to expression. Take time to browse these important mainstays of molecular biology from a company known for its commitment to affordability and exceptional quality.
Showing 1-20 of 26 items.

1 kb DNA Ladder

1 kb DNA Ladder
Catalog ID Size Pricing
D010-500 500 µL $ 46.00
D010-1000 2x500 µL $ 86.00
D010-1500 3x500 µL $ 121.00
D010-2000 4x500 µL $ 161.00
D010-2500 5x500 µL $ 184.00
D010-3000 6x500 µL $ 201.00

Description

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D010
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D010-500 500 µL $ 46.00
D010-1000 2x500 µL $ 86.00
D010-1500 3x500 µL $ 121.00
D010-2000 4x500 µL $ 161.00
D010-2500 5x500 µL $ 184.00
D010-3000 6x500 µL $ 201.00

1 kb PLUS™ DNA Ladder

Catalog ID Size Pricing
D011-500 500 µL $ 58.00
D011-1000 2x500 µL $ 109.00
D011-1500 3x500 µL $ 155.00
D011-2000 4x500 µL $ 196.00
D011-2500 5x500 µL $ 230.00
D011-3000 6x500 µL $ 253.00

Description

The 1 kb PLUS™ is composed of 14 individual DNA fragments: 25K, 10K, 8K, 6K, 5K, 4K, 3K, 2.5K, 2K, 1500, 1000, 750, 500, 250 base pairs. This product contains two enhanced bands (3K and 1K bp) for easy reference. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D011
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D011-500 500 µL $ 58.00
D011-1000 2x500 µL $ 109.00
D011-1500 3x500 µL $ 155.00
D011-2000 4x500 µL $ 196.00
D011-2500 5x500 µL $ 230.00
D011-3000 6x500 µL $ 253.00

100 bp DNA Ladder

Catalog ID Size Pricing
D001-500 500 µL $ 46.00
D001-1000 2x500 µL $ 86.00
D001-1500 3x500 µL $ 121.00
D001-2000 4x500 µL $ 161.00
D001-2500 5x500 µL $ 184.00
D001-3000 6x500 µL $ 201.00

Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D001
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D001-500 500 µL $ 46.00
D001-1000 2x500 µL $ 86.00
D001-1500 3x500 µL $ 121.00
D001-2000 4x500 µL $ 161.00
D001-2500 5x500 µL $ 184.00
D001-3000 6x500 µL $ 201.00

100 bp PLUS™ DNA Ladder

Catalog ID Size Pricing
D003-500 500 µL $ 46.00
D003-1000 2x500 µL $ 86.00
D003-1500 3x500 µL $ 127.00
D003-2000 4x500 µL $ 161.00
D003-2500 5x500 µL $ 184.00
D003-3000 6x500 µL $ 201.00

Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 12 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-3,000 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D003
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D003-500 500 µL $ 46.00
D003-1000 2x500 µL $ 86.00
D003-1500 3x500 µL $ 127.00
D003-2000 4x500 µL $ 161.00
D003-2500 5x500 µL $ 184.00
D003-3000 6x500 µL $ 201.00

5-ethynyl-2’-deoxyuridine (5-EdU)

Catalog ID Size Pricing
E-980-100 100 mg $ 63.00
E-980-250 250 mg $ 105.00
E-980-500 500 mg $ 159.00
E-980-1 1 g $ 239.00

Description

No description available

Product Specifications

Catalog ID E-980
CAS # 61135-33-9
MW 252.22 g/mol
Storage/Handling Store desiccated at -20°C.
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E-980-100 100 mg $ 63.00
E-980-250 250 mg $ 105.00
E-980-500 500 mg $ 159.00
E-980-1 1 g $ 239.00

5-Fluoroorotic acid monohydrate (5-FOA)

Catalog ID Size Pricing
F-230-2.5 2.5 g $ 58.00
F-230-5 5 g $ 110.00
F-230-10 10 g $ 184.00
F-230-25 25 g $ 338.00

Description

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

Product Specifications

Catalog ID F-230
CAS # 220141-70-8
MW 192.10 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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F-230-2.5 2.5 g $ 58.00
F-230-5 5 g $ 110.00
F-230-10 10 g $ 184.00
F-230-25 25 g $ 338.00

50 bp DNA Ladder

50 bp DNA Ladder
Catalog ID Size Pricing
D100-500 500 µL $ 58.00
D100-1000 2x500 µL $ 109.00
D100-1500 3x500 µL $ 155.00
D100-2000 4x500 µL $ 196.00
D100-2500 5x500 µL $ 230.00
D100-3000 6x500 µL $ 253.00

Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 17 fragments, suitable for gel electrophoresis. The DNA includes fragments ranging from 50-1,500 base pairs. The 200 and 500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 g a load) for approximating the mass of DNA in comparably intense samples of a similar size.The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D100
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D100-500 500 µL $ 58.00
D100-1000 2x500 µL $ 109.00
D100-1500 3x500 µL $ 155.00
D100-2000 4x500 µL $ 196.00
D100-2500 5x500 µL $ 230.00
D100-3000 6x500 µL $ 253.00

Agarose LE (Molecular Biology Grade)

Catalog ID Size Pricing
A-201-100 100 g $ 92.00
A-201-500 500 g $ 338.00
A-201-1000 1 kg $ 562.00

Description

Agarose LE (Low Electroendosmosis) is Molecular Biology Grade and is specifically designed for the superior separation of nucleic acids, with maximally crisp band resolution, and is also ideal for cloning and cloning related experiments.

GoldBio Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end-product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 50 bp to 25 kbp, protein electrophoresis and various blotting protocols.

The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

Agarose is a natural product that forms an inert matrix used in electrophoresis, chromatography and other molecular biology and biochemistry techniques. Likewise, it is neutral and easily derivatizable, so it is easy to bind to its structure proteins like enzymes, antigens or antibodies. Toxicity absence makes working with agarose very convenient.

Product Specifications

Catalog ID A-201
CAS # 9012-36-6
Storage/Handling Store at room temperature.
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A-201-100 100 g $ 92.00
A-201-500 500 g $ 338.00
A-201-1000 1 kg $ 562.00

Ampicillin (Sodium), USP Grade

Catalog ID Size Pricing
A-301-5 5 g $ 23.00
A-301-10 10 g $ 34.00
A-301-25 25 g $ 46.00
A-301-100 100 g $ 172.00
A-301-500 500 g $ 822.00

Description

Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains. Ampicillin sodium targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. It has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus species. Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation. Ampicillin Sodium is freely soluble in water.

Product Specifications

Catalog ID A-301
CAS # 69-52-3
MW 371.39 g/mol
Storage/Handling Store desiccated at -20°C.
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A-301-5 5 g $ 23.00
A-301-10 10 g $ 34.00
A-301-25 25 g $ 46.00
A-301-100 100 g $ 172.00
A-301-500 500 g $ 822.00

Carbenicillin (Disodium)

Catalog ID Size Pricing
C-103-5 5 g $ 46.00
C-103-25 25 g $ 150.00
C-103-50 50 g $ 264.00
C-103-100 100 g $ 450.00
C-103-250 250 g $ 911.00

Description

Carbenicillin is a member of the penicillin β-lactam antibiotics. However, unlike most β-lactams, carbenicillin disodium is limited to primarily gram-negative bacteria including Pseudomonas aeruginosa and common enteric species. It can be used in selection protocols and is often preferred over ampicillin due to its increased stability. This allows for less contamination of unwanted bacterial colonies. In addition, carbenicillin has been used to regulate bacterial growth in plants, yielding low regeneration frequencies. It can be used in genetic transformation applications to select for AmpR transformed cells.

Product Specifications

Catalog ID C-103
CAS # 4800-94-6
MW 422.36 g/mol
Grade MOLECULAR BIOLOGY GRADE
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C-103-5 5 g $ 46.00
C-103-25 25 g $ 150.00
C-103-50 50 g $ 264.00
C-103-100 100 g $ 450.00
C-103-250 250 g $ 911.00

Cefotaxime (Sodium), USP Grade

Catalog ID Size Pricing
C-104-1 1 g $ 23.00
C-104-5 5 g $ 41.00
C-104-25 25 g $ 172.00

Description

Cefotaxime is a third generation, broad-spectrum cephalosporin antibiotic effective against various gram-negative and gram-positive bacteria. However, unlike many other cephalosporins, cefotaxime is not effective against Pseudomonas aeruginosa bacteria. Cefotaxime is often used in Agrobacterium tumefaciens-mediated transformations to regulate bacterial growth. It has been shown to exhibit a synergistic effect with Vancomycin in certain conditions.

Product Specifications

Catalog ID C-104
CAS # 64485-93-4
MW 477.45 g/mol
Storage/Handling Store desiccated at -20°C.
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C-104-1 1 g $ 23.00
C-104-5 5 g $ 41.00
C-104-25 25 g $ 172.00

Chloramphenicol, USP Grade

Catalog ID Size Pricing
C-105-5 5 g $ 12.00
C-105-25 25 g $ 23.00
C-105-100 100 g $ 79.00

Description

Chloramphenicol, also known as chlornitromycin, is a broad-spectrum, bacteriostatic antibiotic derived from Streptomyces venezuelae. It is effective against several gram-positive and gram-negative bacteria and commonly used in researching protein synthesis and to select for chloramphenicol-resistant transformed cells or the bacterial CAT gene.

Product Specifications

Catalog ID C-105
CAS # 56-75-7
MW 323.13 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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C-105-5 5 g $ 12.00
C-105-25 25 g $ 23.00
C-105-100 100 g $ 79.00

G-418 Sulfate

Catalog ID Size Pricing
G-418-1 1 g $ 30.00
G-418-5 5 g $ 137.00
G-418-10 10 g $ 229.00
G-418-25 25 g $ 338.00

Description

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. It can be used to select for cells transformed with an iNOS promoter construct or neomycin resistance gene.

G-418 functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 disulfate is freely soluble in water.

Product Specifications

Catalog ID G-418
CAS # 108321-42-2
MW 692.71 g/mol
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G-418-1 1 g $ 30.00
G-418-5 5 g $ 137.00
G-418-10 10 g $ 229.00
G-418-25 25 g $ 338.00

Gentamicin Sulfate, USP Grade

Catalog ID Size Pricing
G-400-1 1 g $ 23.00
G-400-5 5 g $ 34.00
G-400-10 10 g $ 58.00
G-400-25 25 g $ 88.00
G-400-100 100 g $ 282.00

Description

Gentamicin is an aminoglycoside, broad-spectrum antibiotic that effectively inhibits Gram-negative and Gram-positive cell growth.  Gentamicin's mode of action is to bind to the 30S ribosomal subunit and interfere with protein synthesis.

It is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram-positive and gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients.

Product Specifications

Catalog ID G-400
CAS # 1405-41-0
MW 575.67 g/mol
Storage/Handling Store desiccated at -20°C.
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G-400-1 1 g $ 23.00
G-400-5 5 g $ 34.00
G-400-10 10 g $ 58.00
G-400-25 25 g $ 88.00
G-400-100 100 g $ 282.00

High Resolution Agarose (For Nucleotides < 1kb)

Catalog ID Size Pricing
A-202-100 100 g $ 254.00
A-202-500 500 g $ 1,207.00

Description

No description available

Product Specifications

Catalog ID A-202
CAS # 9012-36-6
Storage/Handling Store at room temperature.
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A-202-100 100 g $ 254.00
A-202-500 500 g $ 1,207.00

Hygromycin B

Catalog ID Size Pricing
H-270-1 1 g $ 68.00
H-270-2-1 2 x 1 g $ 127.00
H-270-2.5 2.5 g $ 161.00
H-270-5 5 g $ 281.00
H-270-5-1 5 x 1 g $ 305.00
H-270-10 10 g $ 506.00
H-270-10-1 10 x 1 g $ 518.00

Description

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.

Product Specifications

Catalog ID H-270
CAS # 31282-04-9
MW 527.52 g/mol
Storage/Handling Store desiccated at -20°C.
UN Number 3462
Item Class 6.1
Group Number I
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H-270-1 1 g $ 68.00
H-270-2-1 2 x 1 g $ 127.00
H-270-2.5 2.5 g $ 161.00
H-270-5 5 g $ 281.00
H-270-5-1 5 x 1 g $ 305.00
H-270-10 10 g $ 506.00
H-270-10-1 10 x 1 g $ 518.00

IPTG

Catalog ID Size Pricing
I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00   $ 22.00
I2481C25 25 g $ 115.00   $ 92.00
I2481C50 50 g $ 184.00   $ 148.00
I2481C100 100 g $ 349.00   $ 280.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

Product Specifications

Catalog ID I2481
CAS # 367-93-1
MW 238.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00
$ 22.00
I2481C25 25 g $ 92.00
I2481C50 50 g $ 184.00
$ 148.00
I2481C100 100 g $ 349.00
$ 280.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

IPTG EZ Pak™ for 1 M Solution

Catalog ID Size Pricing
I2481-EZ10 10 mL $ 58.00
I2481-EZ50 50 mL $ 92.00
I2481-EZ100 100 mL $ 150.00

Description

The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

Product Specifications

Catalog ID I2481-EZ
CAS # 367-93-1
MW 238.30 g/mol
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I2481-EZ10 10 mL $ 58.00
I2481-EZ50 50 mL $ 92.00
I2481-EZ100 100 mL $ 150.00

Kanamycin Monosulfate, USP Grade

Catalog ID Size Pricing
K-120-5 5 g $ 23.00
K-120-10 10 g $ 44.00
K-120-25 25 g $ 92.00
K-120-50 50 g $ 173.00
K-120-100 100 g $ 323.00
K-120-250 250 g $ 748.00

Description

Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus. It is effective against gram-positive and gram-negative bacteria as well as Mycoplasma species. Kanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have the NPT II (APH3) gene incorporated in them. Kanamycin is very soluble in aqueous solution and is typically used in concentrations of 50-100 µg/ml.

Product Specifications

Catalog ID K-120
CAS # 25389-94-0
MW 582.58 g/mol
Storage/Handling Store desiccated at -20°C.
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K-120-5 5 g $ 23.00
K-120-10 10 g $ 44.00
K-120-25 25 g $ 92.00
K-120-50 50 g $ 173.00
K-120-100 100 g $ 323.00
K-120-250 250 g $ 748.00

Low Melt Agarose

Catalog ID Size Pricing
A-204-25 25 g $ 119.00
A-204-100 100 g $ 257.00

Description

For applications requiring low gel/melt temperature.
Low Melting (LM) Agarose is a low melting temperature agarose with the highest resolving capacity for large DNA fragments, including PCR products.
Low melting temperature allows for the recovery of undamaged nucleic acids below denaturation temperature. Low gelling temperature ensures In-Gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps. 

Low Melting Agarose is ideal for digestion by agarose enzymes, which makes it very easy to recover large DNA fragments suitable for cloning or enzymatic processing.

Product Specifications

Catalog ID A-204
CAS # 9012-36-6
Storage/Handling Store at room temperature.
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A-204-25 25 g $ 119.00
A-204-100 100 g $ 257.00

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