Cell Selection & Maintenance

Streptomycin is an aminoglycoside antibiotic primarily effective against gram-negative bacteria and Mycobacterium species. It is commonly used in cell culture media to prevent bacterial contamination. It has also been used to precipitate nucleic acids during protein purification techniques and to study mechanisms of Streptomycin resistance.

GoldBio's SuperLadder (50 bp-25 kb) is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct gel loading. The DNA Ladder is composed of 26 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100 and 50 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA.

This product contains four enhanced bands (3k, 1.2k, 500 and 200 bp) for easier reference. In addition, three tracking dyes are present (Xylene cyanol FF, Bromophenol blue and Orange G) which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA respectively during electrophoresis on a 1% TAE agarose gel which helps in real time monitoring.

Timentin™ is a 15:1 mixture of ticarcillin and clavulanate. Ticarcillin is a penicillin ß-lactam antibiotic, which is susceptible to ß-lactamase degradation. When combined with clavulanate, a ß-lactamase inhibitor, its efficacy is greatly increased. Timentin™ exhibits high activity against gram-negative bacteria and resistant Agrobacterium species.

Vancomycin hydrochloride is a member of the glycopeptide antibiotic class derived from Streptomyces orientalis. It is effective against gram-positive bacteria and used in studies of antibiotic resistance bacteria and nanoparticle transport studies. Exhibiting low toxicity to plants, vancomycin is suitable for plant selection and prevention of bacterial contamination in plant cell culture applications.

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 19 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-10,000 base pairs. The 500, 1.5K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.