Cloning & Induction

Gold Biotechnology offers a variety of lysis buffers fit for bacterial, animal, plant or yeast cultures, as well as IPTG, which is also available in an easy to use EZ Pak, and 5-Fluoroorotic Acid, ideal for yeast selection. These tools are fundamental for induction, selection and protein purification. GoldBio carries quality products at a fraction of the price of other suppliers, allowing you to focus your research funds on the things that really matter.
Showing 1-20 of 35 items.

IPTG

Catalog ID Size Pricing
I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00
I2481C25 25 g $ 115.00
I2481C50 50 g $ 184.00
I2481C100 100 g $ 349.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

Product Specifications

Catalog ID I2481
CAS # 367-93-1
MW 238.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00
I2481C25 25 g $ 115.00
I2481C50 50 g $ 184.00
I2481C100 100 g $ 349.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Hot Start Taq DNA Polymerase

There is no image for Hot Start Taq DNA Polymerase Hot Start Taq DNA Polymerase
Catalog ID Size Pricing
T-510-200 200 µL $ 258.00
T-510-40 40 µL $ 62.00

Description

Hot Start Taq DNA Polymerase is a chemically modified enzyme that remains inactive at room temperature and requires an increase in temperature for activation.

Product Specifications

Catalog ID T-510
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T-510-200 200 µL $ 258.00
T-510-40 40 µL $ 62.00

T4 DNA Helicase (gp41)

There is no image for T4 DNA Helicase (gp41) T4 DNA Helicase (gp41)
Catalog ID Size Pricing
T-417-100 100 µg $ 361.00
T-417-500 500 µg $ 1,056.00

Description

No description available

Product Specifications

Catalog ID T-417
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T-417-100 100 µg $ 361.00
T-417-500 500 µg $ 1,056.00

5-Fluoroorotic acid monohydrate (5-FOA)

Catalog ID Size Pricing
F-230-2.5 2.5 g $ 58.00
F-230-5 5 g $ 110.00
F-230-10 10 g $ 184.00
F-230-25 25 g $ 338.00

Description

5-Fluoroorotic Acid (5-FOA) is widely used in the field of yeast molecular genetics. The toxicity of 5-FOA to yeast cells with a functioning URA3 gene which encodes orotidine-5′-P decarboxylase (OMP decarboxylase), makes it especially useful for the selection and identification of mutant yeast strains. 5-FOA is most often applied with Saccharomyces cerevisiae (URA3), although it has been used in other strains including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3) and the bacterium E. coli (pyrF). The use of 5-FOA in the yeast 2-hybrid system allows for the construction of activation domain hybrid libraries to identify protein-protein interactions.

Product Specifications

Catalog ID F-230
CAS # 220141-70-8
MW 192.10 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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F-230-2.5 2.5 g $ 58.00
F-230-5 5 g $ 110.00
F-230-10 10 g $ 184.00
F-230-25 25 g $ 338.00

IPTG EZ Pak™ for 1 M Solution

Catalog ID Size Pricing
I2481-EZ10 10 mL $ 58.00
I2481-EZ50 50 mL $ 92.00
I2481-EZ100 100 mL $ 150.00

Description

The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

Product Specifications

Catalog ID I2481-EZ
CAS # 367-93-1
MW 238.30 g/mol
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I2481-EZ10 10 mL $ 58.00
I2481-EZ50 50 mL $ 92.00
I2481-EZ100 100 mL $ 150.00

Mammalian Cell Lysis Buffer

Catalog ID Size Pricing
GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Description

Product Specifications

Catalog ID GB-180
Storage/Handling Store at 4°C.
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GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Tissue Cell Lysis Buffer

Catalog ID Size Pricing
GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Description

No description available

Product Specifications

Catalog ID GB-181
Storage/Handling Store at 4°C.
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GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Bacterial Cell Lysis Buffer

Catalog ID Size Pricing
GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-178 100 Preps $ 171.00

Description

No description available

Product Specifications

Catalog ID GB-177
Storage/Handling Store at 4°C. Do NOT freeze.
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GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-178 100 Preps $ 171.00

Yeast Lysis Buffer Kit plus Zymolyase

Catalog ID Size Pricing

Description

No description available

Product Specifications

Catalog ID GB-178
Storage/Handling Store at -20°C.
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Yeast Spheroplast Lysis Buffer

Catalog ID Size Pricing
GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

Description

No description available

Product Specifications

Catalog ID GB-179
Storage/Handling Store at -20°C.
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GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

Cas9 Nuclease (1µM)

There is no image for Cas9 Nuclease (1µM) Cas9 Nuclease (1µM)
Catalog ID Size Pricing
C-519-250 250 µL $ 93.00

Description

No description available

Product Specifications

Catalog ID C-519
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C-519-250 250 µL $ 93.00

Cas9 Nuclease (10µM)

There is no image for Cas9 Nuclease (10µM) Cas9 Nuclease (10µM)
Catalog ID Size Pricing
C-529-50 50 µL $ 155.00
C-529-250 250 µL $ 510.00

Description

No description available

Product Specifications

Catalog ID C-529
Storage/Handling Store at -20°C.
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C-529-50 50 µL $ 155.00
C-529-250 250 µL $ 510.00

Hot Start Taq DNA Polymerase plus dNTP

There is no image for Hot Start Taq DNA Polymerase plus dNTP Hot Start Taq DNA Polymerase plus dNTP
Catalog ID Size Pricing
T-511-40 40 µL $ 76.00
T-511-200 200 µL $ 304.00

Description

No description available

Product Specifications

Catalog ID T-511
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T-511-40 40 µL $ 76.00
T-511-200 200 µL $ 304.00

Hot Start Taq 2x Master Mix - 50 µL reaction

There is no image for Hot Start Taq 2x Master Mix - 50 µL reaction Hot Start Taq 2x Master Mix - 50 µL reaction
Catalog ID Size Pricing
T-512-100 100 reactions $ 72.00
T-512-500 500 reactions $ 216.00

Description

No description available

Product Specifications

Catalog ID T-512
Storage/Handling Store at -20°C.
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T-512-100 100 reactions $ 72.00
T-512-500 500 reactions $ 216.00

Hot Start Taq 2x Master Mix - 20 µL reaction

There is no image for Hot Start Taq 2x Master Mix - 20 µL reaction Hot Start Taq 2x Master Mix - 20 µL reaction
Catalog ID Size Pricing
T-513-100 100 reactions $ 41.00
T-513-500 500 reactions $ 134.00

Description

No description available

Product Specifications

Catalog ID T-513
Storage/Handling Store at -20°C.
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T-513-100 100 reactions $ 41.00
T-513-500 500 reactions $ 134.00

Taq DNA Polymerase

There is no image for Taq DNA Polymerase Taq DNA Polymerase
Catalog ID Size Pricing
T-514-500 500 µL $ 72.00
T-514-5 5 mL $ 510.00

Description

No description available

Product Specifications

Catalog ID T-514
Storage/Handling Store at -20°C.
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T-514-500 500 µL $ 72.00
T-514-5 5 mL $ 510.00

Taq DNA Polymerase plus dNTP

There is no image for Taq DNA Polymerase plus dNTP Taq DNA Polymerase plus dNTP
Catalog ID Size Pricing
T-515-500 500 µL $ 88.00
T-515-5 5 mL $ 613.00

Description

No description available

Product Specifications

Catalog ID T-515
Storage/Handling Store at -20°C.
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T-515-500 500 µL $ 88.00
T-515-5 5 mL $ 613.00

Taq DNA Polymerase with Dye

There is no image for Taq DNA Polymerase with Dye Taq DNA Polymerase with Dye
Catalog ID Size Pricing
T-516-500 500 µL $ 77.00
T-516-5 5 mL $ 520.00

Description

No description available

Product Specifications

Catalog ID T-516
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T-516-500 500 µL $ 77.00
T-516-5 5 mL $ 520.00

Taq DNA Polymerase with Dye plus dNTP

There is no image for Taq DNA Polymerase with Dye plus dNTP Taq DNA Polymerase with Dye plus dNTP
Catalog ID Size Pricing
T-517-500 500 µL $ 93.00
T-517-5 5 mL $ 623.00

Description

No description available

Product Specifications

Catalog ID T-517
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T-517-500 500 µL $ 93.00
T-517-5 5 mL $ 623.00

Taq DNA Polymerase 2x Premix with Dye

There is no image for Taq DNA Polymerase 2x Premix with Dye Taq DNA Polymerase 2x Premix with Dye
Catalog ID Size Pricing
T-518-100 100 reactions $ 67.00
T-518-500 500 reactions $ 139.00

Description

No description available

Product Specifications

Catalog ID T-518
Storage/Handling Store at -20°C.
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T-518-100 100 reactions $ 67.00
T-518-500 500 reactions $ 139.00

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