Cobalt

Cobalt agarose beads are used in His-tag purifications. GoldBio’s IDA cross-linked agarose resins consist of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. Cobalt divalent metal ions have a high specificity and low affinity binding capacity for the best purification of specific proteins from a protein extract. Cobalt has a binding capacity of 6-12 mg/ml with a ~30 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. GoldBio’s cobalt agarose beads are a high density form that helps to improve the affinity of the purification.
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Cobalt Agarose Beads (High Density)

Catalog ID Size Pricing
H-310-5 5 mL $ 53.00
H-310-10 10 mL $ 92.00
H-310-25 25 mL $ 205.00
H-310-50 50 mL $ 341.00
H-310-100 100 mL $ 609.00
H-310-500 500 mL $ 2,494.00

Description

Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.

Product Specifications

Catalog ID H-310
Storage/Handling store at 4°C. Do NOT freeze.
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H-310-5 5 mL $ 53.00
H-310-10 10 mL $ 92.00
H-310-25 25 mL $ 205.00
H-310-50 50 mL $ 341.00
H-310-100 100 mL $ 609.00
H-310-500 500 mL $ 2,494.00

Cobalt HTC Agarose Beads

Catalog ID Size Pricing
R-203-25 25 mL $ 291.00
R-203-100 100 mL $ 735.00
R-203-500 500 mL $ 2,823.00

Description

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

Product Specifications

Catalog ID R-203
Storage/Handling store at 4°C. Do NOT freeze.
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R-203-25 25 mL $ 291.00
R-203-100 100 mL $ 735.00
R-203-500 500 mL $ 2,823.00

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