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Competent Cells and Transformation
GoldBio offers an extensive collection of efficient chemically competent and electrocompetent cells with high transformation efficiencies to match your specific downstream applications: general cloning, protein expression, library construction, and many more.
- GoldBio's wide range of chemically competent and electrocompetent cells are optimized to provide lot-to-lot consistency and up to 10X higher transformation efficiency.
- Our universal recovery medium (SOC) formulation accompanies ALL of GoldBio's competent cells.
- We carry multiple Endonuclease I-deficient (endA1) competent bacterial cell strains to suit your cloning needs and ensure your transformations result in high quality and yield of plasmid DNA.
- Choose from E. coli strains with different selectable markers and screening options including the classic blue/white selection.
- GoldBio cells are ideal for distinct downstream applications from routine cloning/subcloning and protein expression to the more complex library construction, and cloning of difficult targets.
- Our electrocompetent Agrobacterium cells contain an efficient T-DNA binary system for genetic material transfer into the host plant cells.
CHOOSE THE BEST COMPETENT CELLS FOR YOUR APPLICATION!
Our GB5-alpha™, BL21, BL21(DE3), GB10B™, GB10B-Pro™, DL39(DE3), and Agrobacterium cells are available in multiple formats to provide you with flexibility when planning your experiments. GoldBio also provides easy-to-follow protocols, FAQs, protocol videos and selection guides to ensure success of your transformations!
Use GoldBio's Competent Cell Guide to Select the Right Competent Cells for your Assay
Includes transformation efficiencies, genotype, strain information, screening methods and applicatio...
Read more!
BROWSE OUR TRANSFORMATION AND COMPETENT CELL RESOURCES
Your time in the lab is valuable! In this section you will find GoldBio's transformation tools designed to help support and simplify your competent cell transformations. You can access specific chemical transformation or electroporation protocols for each of our competent cell lines, antibiotic information, colony screening protocols, instructional blog articles, detailed guides containing competent cells characteristics and downstream applications, FAQs, electroporation troubleshooting tips and video protocols.
Electroporation and Electrocompetent Cells FAQs:
What is electroporation?
Electroporation is a technique commonly used in transformation assays to introduce DNA into an electrocompetent cell. During electroporation a transient current or electrical pulse is applied to cells that have been suspended in a solution. The current momentarily increases the permeability of the cell's membrane, creating holes (a pathway) that allow DNA (plasmids) to pass through and enter the cell. Generally, electroporation can be more effective than chemical transformation.
When should I use electrocompetent cells?
Electrocompetent cells have a higher transformation efficiency than chemically competent cells. The use of electrocompetent cells facilitate the transformation of large plasmids, including BACs (Bacterial Artificial Chromosomes), that might otherwise be difficult to transform using chemical transformation.
How do I prevent arcing during electroporation?
Arcing during electroporation can be avoided by making sure there are no air bubbles in the cell/DNA solution prior to electroporation. Also, desalt your DNA since the presence of conductive ions in the solution will cause arcing and electroporation failure. In addition, ensure that your equipment, including the cuvette is properly cleaned and in good condition.
Showing 21-40 of 43 items.
GB10B-Pro™ Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-201-5x50 | 5 x 50 μL |
|
$ 96.00 |
| CC-201-10x50 | 10 x 50 μL |
|
$ 172.00 |
| CC-201-5x100 | 5 x 100 μL |
|
$ 172.00 |
| CC-201-15x50 | 15 x 50 μL |
|
$ 236.00 |
| CC-201-10x100 | 10 x 100 μL |
|
$ 464.00 |
| CC-201-15x100 | 15 x 100 μL |
|
$ 674.00 |
Description
GoldBio’s GB10B-Pro™ Electrocompetent E. coli cells are especially designed for the most demanding cloning applications. GB10B-Pro™ cells will provide the greatest number of transformants for when your research requires it, including assembling large and multi-DNA fragments, cloning large (≥10 kb up to 350 kb) or difficult construct transformations, working with synthetic bio-applications, and even BAC cloning.
Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH10B
Species: E. coli
Transformation efficiency:≥1 x 109 cfu/µg pUC19 DNA
Blue/white screening: Yes
GB10B™ Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-100-5x50 | 5 x 50 μL |
|
$ 48.00 |
| CC-100-5x100 | 5 x 100 μL |
|
$ 84.00 |
| CC-100-10x50 | 10 x 50 μL |
|
$ 84.00 |
| CC-100-15x50 | 15 x 50 μL |
|
$ 112.00 |
| CC-100-20x50 | 20 x 50 μL |
|
$ 132.00 |
| CC-100-10x100 | 10 x 100 μL |
|
$ 144.00 |
| CC-100-15x100 | 15 x 100 μL |
|
$ 192.00 |
| CC-100-20x100 | 20 x 100 μL |
|
$ 244.00 |
Description
GoldBio’s GB10B™ Chemically Competent E. coli cells are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. GB10B™ Chemically Competent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine.
Product Specifications
Competent cell type: Chemically competent
Equivalent to: DH10B
Species: E. coli
Transformation efficiency: ≥8.2 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes
GB10B™ Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-200-5x50 | 5 x 50 μL |
|
$ 96.00 |
| CC-200-5x100 | 5 x 100 μL |
|
$ 148.00 |
| CC-200-10x50 | 10 x 50 μL |
|
$ 172.00 |
| CC-200-15x50 | 15 x 50 μL |
|
$ 236.00 |
| CC-200-10x100 | 10 x 100 μL |
|
$ 256.00 |
| CC-200-15x100 | 15 x 100 μL |
|
$ 375.00 |
Description
GoldBio’s GB10B™ Electrocompetent E. coli cells are high efficiency cells, ≥2 x 108 cfu/µg plasmid DNA, suitable for a wide variety of applications requiring high transformation efficiencies, such as cloning and subcloning, as well as working with either cDNA or gDNA library construction. GB10B™ Electrocompetent E. coli cells have multiple features including the ɸ80lacZ∆M15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol. These cells also have the mcrA genotypic marker and the mcrBC, mrr deletion, which allows for cloning of DNA that contains methylcytosine and methyladenine. Here, we present a detailed protocol for electroporation using GB10B™ Electrocompetent E. coli cells.
Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH10B
Species: E. coli
Transformation efficiency: ≥2 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
| Catalog ID | CC-200 |
|---|---|
| Storage/Handling | Store Competent Cells at -80°C. |
GB5-alpha™ Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-101-5x50 | 5 x 50 μL |
|
$ 48.00 |
| CC-101-5x100 | 5 x 100 μL |
|
$ 84.00 |
| CC-101-10x50 | 10 x 50 μL |
|
$ 84.00 |
| CC-101-15x50 | 15 x 50 μL |
|
$ 112.00 |
| CC-101-20x50 | 20 x 50 μL |
|
$ 132.00 |
| CC-101-10x100 | 10 x 100 μL |
|
$ 144.00 |
| CC-101-15x100 | 15 x 100 μL |
|
$ 194.00 |
Description
GoldBio’s GB5-alpha™ Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, cloning, and subcloning.
Product Specifications
Competent cell type: Chemically Competent
Equivalent to: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 106 cfu/µg pUC19 DNA
Blue/white screening: Yes
GB5-alpha™ Electrocompetent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-203-5x50 | 5 x 50 μL |
|
$ 96.00 |
| CC-203-5x100 | 5 x 100 μL |
|
$ 148.00 |
| CC-203-10x50 | 10 x 50 μL |
|
$ 172.00 |
| CC-203-15x50 | 15 x 50 μL |
|
$ 236.00 |
| CC-203-10x100 | 10 x 100 μL |
|
$ 252.00 |
| CC-203-15x100 | 15 x 100 μL |
|
$ 320.00 |
Description
GoldBio’s GB5-alpha™ Electrocompetent E. coli are high efficiency cells, suitable for a wide variety of applications such as cloning and sub-cloning. Plasmid quality and yields are both enhanced in our GB5-alpha™ cells due to mutations in both endA1 and recA1.
Product Specifications
Competent cell type: ElectroCompetent
Equivalent to: DH5-alpha
Species: E. coli
Transformation efficiency: ≥5 x 108 cfu/µg pUC19 DNA
Blue/white screening: Yes
GV3101 (pSoup) Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-115-5x50 | 5 x 50 μL |
|
$ 152.00 |
| CC-115-10x50 | 10 x 50 μL |
|
$ 268.00 |
| CC-115-15x50 | 15 x 50 μL |
|
$ 360.00 |
Description
GoldBio’s GV3101 Agrobacterium chemically competent cells are a powerful tool for high efficiency transformation of plants. The GV3101 strain is optimized for cDNA and gDNA library construction, and possesses resistance to rifampicin, gentamicin, and tetracycline. The T-DNA region of the GV3101 Ti plasmid has been deleted, but restoration of the binary vector T-region can fully enable T-DNA binary system function.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101 (pSoup)
Transformation efficiency: ≥1.5 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup) Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-217-5x50 | 5 x 50 μL |
|
$ 160.00 |
| CC-217-10x50 | 10 x 50 μL |
|
$ 288.00 |
| CC-217-15x50 | 15 x 50 μL |
|
$ 388.00 |
Description
GoldBio’s GV3101 Agrobacterium electrocompetent cells are a powerful tool for high efficiency transformation of plants. The GV3101 strain is optimized for cDNA and gDNA library construction, and possesses resistance to rifampicin, gentamicin, and tetracycline. The T-DNA region of the GV3101 Ti plasmid has been deleted, but restoration of the binary vector T-region can fully enable T-DNA binary system function.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101 (pSoup)
Transformation efficiency: ≥8 x 107 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup-P19) Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-125-5x50 | 5 x 50 μL |
|
$ 152.00 |
| CC-125-10x50 | 10 x 50 μL |
|
$ 268.00 |
| CC-125-15x50 | 15 x 50 μL |
|
$ 360.00 |
Description
GoldBio’s GV3101 competent Agrobacterium cells are a highly efficient tool for genetic transformation of dicotyledonous and monocotyledonous plant species, such as Arabidopsis thaliana, tobacco, potato, and corn. These cells carry the nopaline-type Ti plasmid pMP90 (pTiC58DT-DNA) that contains the vir genes required for the transfer and integration of T-DNA into the plant genome. Additionally, the cells are resistant to rifampicin, gentamicin, and tetracycline, which allows for selection of transformed cells.
The pSoup plasmid carried by the cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes. These combined properties make GV3101 competent Agrobacterium cells an ideal choice for cDNA or gDNA library construction and the generation of transgenic plants with desirable traits.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101
(pSoup-P19)
Transformation efficiency: ≥3 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 (pSoup-P19) Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-227-5x50 | 5 x 50 μL |
|
$ 160.00 |
| CC-227-10x50 | 10 x 50 μL |
|
$ 288.00 |
| CC-227-15x50 | 15 x 50 μL |
|
$ 388.00 |
Description
GoldBio’s GV3101 electrocompetent Agrobacterium cells are a highly efficient tool for genetic transformation of dicotyledonous and monocotyledonous plant species, such as Arabidopsis thaliana, tobacco, potato, and corn. These cells carry the nopaline-type Ti plasmid pMP90 (pTiC58DT-DNA) that contains the vir genes required for the transfer and integration of T-DNA into the plant genome. Additionally, the cells are resistant to rifampicin, gentamicin, and tetracycline, which allows for selection of transformed cells.
The pSoup plasmid carried by the cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign genes. These combined properties make GV3101 electrocompetent Agrobacterium cells an ideal choice for cDNA or gDNA library construction and the generation of transgenic plants with desirable traits.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101 (pSoup-P19)
Transformation efficiency: ≥1 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-105-5x50 | 5 x 50 μL |
|
$ 152.00 |
| CC-105-10x50 | 10 x 50 μL |
|
$ 268.00 |
| CC-105-15x50 | 15 x 50 μL |
|
$ 360.00 |
Description
GoldBio’s GV3101 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: Chemically Competent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥6 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
GV3101 Agrobacterium Electrocompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-207-5x50 | 5 x 50 μL |
|
$ 160.00 |
| CC-207-10x50 | 10 x 50 μL |
|
$ 288.00 |
| CC-207-15x50 | 15 x 50 μL |
|
$ 388.00 |
Description
GoldBio’s GV3101 Agrobacterium Electrocompetent Cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) harboring the gentamicin resistance. A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted from the Ti plasmid and instead has a binary vector containing the missing T-region. The binary system makes possible to transfer genetic material into a host plant’s genome. Our system is often used for Agrobacterium-mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana, tobacco, potato, soybeans and corn.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: GV3101
Transformation efficiency: ≥2 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
HB101 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-150-5x50 | 5 x 50 µl |
|
$ 48.00 |
| CC-150-10x50 | 10 x 50 µl |
|
$ 84.00 |
Description
GoldBio’s HB101 chemically competent E. coli cells allow you to obtain high transformation efficiency in applications such cloning and sub-cloning. Our E. coli HB101 proceeds from the K12 x B hybrid strain. HB101 strain also contains the recA13 mutation useful in the insert stability and minimization of recombination. E. coli HB101 also has the hsdS20(rB-mB-) genotype that prevents the cleavage of cloned DNA by endogenous restriction enzymes.
Product Specifications
Competent cell type: Chemically competent
Derivative of: HB101
Species: E. coli
Transformation efficiency: ≥7 x 106 cfu/µg pUC19 DNA
Blue/white screening: No
JM109 Chemically Competent E. coli Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-114-5x50 | 5 x 50 µl |
|
$ 48.00 |
| CC-114-10x50 | 10 x 50 µl |
|
$ 84.00 |
Description
GoldBio’s JM109 Chemically Competent E. coli cells are suitable for high efficiency transformation in a wide variety of routine applications such as plasmid isolation, library construction, cloning, and subcloning. JM109 is a K strain bacterium that carries multiple features including the lacIqZΔM15 marker, which provides α-complementation of the β-galactosidase gene with blue/white screening protocol as well as the hsdR17 genotype which prevents endogenous endonucleases from cleaving DNA.
Product Specifications
Competent cell type: Chemically competent
Derivative of: JM109
Species: E. coli
Format: Tubes
Transformation efficiency: ≥6 x 105 cfu/µg pUC19 DNA
Blue/white screening: Yes
Product Specifications
| Catalog ID | CC-114 |
|---|---|
| Storage/Handling | Store Competent Cells at -80°C. |
LBA4404 Agrobacterium Chemically Competent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-107-5x50 | 5 x 50 μL |
|
$ 152.00 |
| CC-107-10x50 | 10 x 50 μL |
|
$ 268.00 |
| CC-107-15x50 | 15 x 50 μL |
|
$ 360.00 |
Description
GoldBio’s LBA4404 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbors a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥1 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
LBA4404 Agrobacterium ElectroCompetent Cells
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| CC-220-5x50 | 5 x 50 μL |
|
$ 160.00 |
| CC-220-10x50 | 10 x 50 μL |
|
$ 288.00 |
| CC-220-15x50 | 15 x 50 μL |
|
$ 388.00 |
Description
GoldBio’s LBA4404 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our LBA4404 strain harbor a rifampicin resistance (rif) gene. Furthermore, LBA4404 has an octoprine-type Ti plasmid pAL4404 without self-transport function, containing the vir genes. Our LBA4404 strain can be used in genetic transformation of tomato, tobacco and other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: LBA4404
Transformation efficiency: ≥4 x 106 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
pACYC Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-555-10 | 10 μg | $ 25.00 |
Description
The pACYC plasmid series is another set of commonly used cloning vectors in molecular biology and biotechnology. PACYC184 has a p15A origin of replication with chloramphenicol and tetracycline resistance genes. Unlike the pUC or pBR325 vectors, pACYC184 plasmids are characterized by a low copy number. This means fewer copies of the plasmid are present in each bacterial cell, which can be advantageous for cloning and expressing genes that are toxic to the host cells when present in high quantities. pACYC184 has a small size of 4245 bp and is suitable for cloning small to medium-sized DNA fragments. PACYC plasmids are especially useful in experiments where multiple plasmids are required within the same cell, or when cloning genes that are deleterious to host cells at high copy numbers.
PBR325 Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-595-10 | 10 μg | $ 25.00 |
Description
The pBR325 plasmid is a classic cloning vector used in molecular biology. It is derived from the pBR322 plasmid, which itself is one of the first and most widely used plasmids for cloning purposes.
pCAMBIA1391z Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-590-10 | 10 μg | $ 199.00 |
Description
pCambia1391Z is a reliable vector plasmid derived from pPZP and is a robust tool for genetic manipulation in plants. Versatile and stable, pCambia1391Z adapts to a wide array of plant species, maintaining the integrity of inserted DNA throughout transformation and regeneration. Designed specifically for promoter testing in plants, this vector harbors a promoterless version of gusA for tracking and visualizing transformed cells. It is additionally coupled with a catalase intron downstream of a truncated lacZ gene, enabling blue/white screening in suitable E. coli host strains.
pSoup Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| 1 μg | P-520-1 | $ 195.00 |
Description
The pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. pSoup plasmids are instrumental in helping with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
pSoup-P19 Plasmid
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-525-1 | 1 μg | $ 195.00 |
Description
The pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. The combination of these features in the pSoup-P19 plasmid helps to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
