Gold Biotechnology's dyes and stains are used in numerous biological applications, including protein staining, electrophoresis, bacterial classification and Western staining. GoldBio’s catalog of high-quality dyes include Basic Fuschin, Brilliant Green, Bromocresol Green, Bromophenol Blue, Coomassie Stains, Crystal Violet, Orange G, Ponceau S and Xylenol Blue, among others. GoldBio guarantees high-quality products with excellent quality control and at a price that means you can focus your research money on your research.
Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family and is used as a common analytical dye in SDS-PAGE and BN-PAGE. CBBG can also be used with the Bradford Method to determine protein concentration.
DAB tetrahydrochloride hydrate is a water soluble form of 3-3'-diaminobenzidine and is used to stain for peroxidase activity. The intensity of DAB staining can be increased with the addition of cobalt, nickel or silver ions.
p-Iodonitrotetrazolium Violet (also called INT) is a tetrazolium dye that turns purple upon reduction. INT dye is often used to visualize dehydrogenase activity histochemically as well as western blot staining and as a staining agent for soft agar colony formation assays.
Methylene blue, chloride trihydrate is a stain that is useful in many staining techniques such as Wright's stain, Jenner's stain, Mallory's phloxine-methylene blue stain, and Levin & Black's stain. Methylene blue can also be used to distinguish between living and non-living cells since it is not able to penetrate living cells.
Neutral red dye is a dark brown to dark green power used to stain nuclei red, in cell viability tests, and as a pH indicator (transition of pH indicator: pH 6.8-8.0, red to yellow). Additionally, it is used to prepare neutral red paper and as a histological stain for lysozymes.
Ponceau S is the sodium salt of a diazo dye used as a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF. It is often used in the staining of polypeptides to be later blot-sequenced because it does not adversely affect the sequencing of the blotted peptides. The stain is also reversible with water washes, preferable for immunological detection, and can even be completely removed. Ponceau S is a negatively charged stain which binds to positively charged amino acids as well as non-polar regions of proteins. The limit of detection for Ponceau S is ~250 ng of protein.
Ponceau S is the sodium salt of a diazo dye used as a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF.It is often used in the staining of polypeptides to be later blot-sequenced because it does not adversely affect the sequencing of the blotted peptides.The stain is also reversible with water washes, preferable for immunological detection, and can even be completely removed.Ponceau S is a negatively charged stain which binds to positively charged amino acids as well as non-polar regions of proteins. The limit of detection for Ponceau S is ~250 ng of protein.
Thiazolyl blue tetrazolium bromide (often called MTT) is a common dye used in Cell Proliferation assays or Cell Growth assays. MTT is converted to water-insoluble, dark blue MTT-formazan by mitochondrial dehydrogenases of living cells. The intensity of the color can then be measured colorimetrically at a wavelength of 570 nm.
Store desiccated at 4°C. Protect from light and air.
Xylenol Blue is a useful alternative to thymol blue developed in the 1920s. Xylenol Blue can be used at half the concentration that thymol blue requires. It is functional at the same two pH ranges, acidic (pH 1-3), which transitions from red to yellow, and basic (pH 8-10), which transitions from yellow to blue. Xylenol Blue may be substituted for any experiment that requires thymol blue.
Gold Biotechnology (U.S. Registration No 3,257,927) and Goldbio (U.S. Registration No 3,257,926)
are registered trademarks of Gold Biotechnology, Inc.