E_Products
DL39 (DE3) Chemically Competent E. coli Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-104-5x50 | 5 x 50 μL |
|
$ 48.00 |
CC-104-10x50 | 10 x 50 μL |
|
$ 84.00 |
Description
GoldBio’s DL39 (DE3) Chemically Competent E. coli cells are engineered with a T7 expression system to transform and express proteins in order to label residues such as phenylalanine or leucine. DL39 (DE3) Chemically Competent E. coli cells are deficient in the aromatic (TyrB), branched-chain (JIvE), and aspartate (AspC) transaminases and are auxotrophic for aspartic acid, isoleucine, leucine, phenylananine, tyrosine, and valine residues.
Product Specifications
Competent cell type: Chemically Competent
Derivative of: DL39
Species: E. coli
Transformation efficiency: ≥1 x 107 cfu/µg pUC19 DNA
Blue/white screening: Yes
DTNB (Ellman's Reagent)
Catalog ID | Size | Pricing | |
---|---|---|---|
D-250-5 | 5 g | $ 52.00 | |
D-250-25 | 25 g | $ 152.00 | |
D-250-50 | 50 g | $ 229.00 | |
D-250-100 | 100 g | $ 343.00 |
Description
Yellow crystalline powder that is used for the colorimetric determination of thiol groups in biological samples. DTNB is fairly soluble in water.
Product Specifications
Catalog ID | D-250 |
---|---|
CAS # | 69-78-3 |
MW | 396.36 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at 4°C. Protect from light and heat. |
E-64
Catalog ID | Size | Pricing | |
---|---|---|---|
E-064-5 | 5 mg | $ 99.00 | |
E-064-25 | 25 mg | $ 155.00 | |
E-064-100 | 100 mg | $ 449.00 |
Description
E-64 (trans-Epoxysuccinyl-L-leucylamido(4-guanidino)butane) irreversibly inhibits cysteine proteases without affecting other enzymes that contain cysteine residues. E-64 is capable of inhibiting bromelain, trypsin, papain, staphopain, calpain, collagenase, ficain, cathepsin B, H and L. Inhibition of serine proteases is not seen with E-64, excepting trypsin.
E-64 is usually used in a concentration range of 1 to 10μM. It is widely used for in vivo studies due to its cell permeability, low toxicity, and potency. E-64 is being investigated for the treatment of diseases caused by cysteine protease activity. It has been observed to inhibit programmed cell death and restore antigen and mitogen activity related to normal proliferative responses in T cells obtained from HIV+ donors.
Product Specifications
Catalog ID | E-064 |
---|---|
CAS # | 66701-25-5 |
MW | 357.41 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at -20°C. |
Econazole Nitrate
Catalog ID | Size | Pricing | |
---|---|---|---|
E-200-5 | 5 g | $ 70.00 | |
E-200-25 | 25 g | $ 254.00 | |
E-200-100 | 100 g | $ 669.00 |
Description
Econazole Nitrate is a broad-spectrum antimycotic imidazole antifungal targeting the cell membrane of fungi. It increases cell permeability via ergosterol synthesis inhibition. It is useful against fungi that cause Tinea especially Trichophyton and Microsporum species. It can be used to study platelet serotonin uptake and the synthesis of prostanoids.
Product Specifications
Catalog ID | E-200 |
---|---|
CAS # | 24169-02-6 |
MW | 444.70 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
EDTA Disodium, dihydrate
Catalog ID | Size | Pricing | |
---|---|---|---|
E-210-100 | 100 g | $ 39.00 | |
E-210-500 | 500 g | $ 55.00 | |
E-210-1 | 1 kg | $ 89.00 | |
E-210-2 | 2 x 1 kg | $ 165.00 | |
E-210-5 | 5 kg | $ 385.00 |
Description
EDTA (Ethylenediaminetetraacetic acid) is a chelating agent, a general chemical and a sequestrant. In molecular biology applications, it is used to minimize metal ion contamination and prevent enzymatic activity.
EDTA disodium is routinely used in electrophoresis DNA and protein separation applications to chelate metal ions required for enzymatic activity that could potentially damage DNA and protein structure.
Product Specifications
Catalog ID | E-210 |
---|---|
CAS # | 6381-92-6 |
MW | 372.24 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store desiccated at room temperature. |
EGF, Human
Catalog ID | Size | Pricing | |
---|---|---|---|
1150-04-100 | 100 μg | $ 85.00 | |
1150-04-500 | 500 μg | $ 109.00 | |
1150-04-1000 | 1000 μg | $ 119.00 |
Description
EGF (Epidermal Growth Factor) is a family of growth factors that are derived from membrane-anchored precursors. The family is characterized by the presence of at least one “EGF-like domain” (characterized by the presence of three disulfide bonds, formed from 6 conserved cysteine residues) in their extracellular domain. EGF was originally discovered as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition and stunting of growth when injected into newborn mice. Mature human EGF shares 70% amino acid sequence identity with mature mouse and rat EGF. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and is sometimes called urogastrone.
Product Specifications
Catalog ID | 1150-04 |
---|---|
MW | ~6.2 kDa |
Storage/Handling | Store desiccated at -20°C. |
EGF, Murine
Catalog ID | Size | Pricing | |
---|---|---|---|
1350-04-100 | 100 μg | $ 115.00 | |
1350-04-500 | 500 μg | $ 165.00 |
Description
EGF (Epidermal Growth Factor) is a family of growth factors that are derived from membrane-anchored precursors. The family is characterized by the presence of at least one “EGF-like domain” (characterized by the presence of three disulfide bonds, formed from 6 conserved cysteine residues) in their extracellular domain. EGF was originally discovered as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition and stunting of growth when injected into newborn mice. Additionally, EGF has been shown to inhibit gastric secretion, is involved in wound healing and signals through a receptor known as c-erbB (a class I tyrosine kinase receptor).
Product Specifications
Catalog ID | 1350-04 |
---|---|
MW | ~6.0 kDa |
Storage/Handling | Store desiccated at -20°C. |
EGF, Rat
Catalog ID | Size | Pricing | |
---|---|---|---|
1550-04-100 | 100 μg | $ 305.00 | |
1550-04-500 | 500 μg | $ 599.00 |
Description
EGF (Epidermal Growth Factor) is a family of growth factors that are derived from membrane-anchored precursors. The family is characterized by the presence of at least one “EGF-like domain” (characterized by the presence of three disulfide bonds, formed from 6 conserved cysteine residues) in their extracellular domain. EGF was originally discovered as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition and stunting of growth when injected into newborn mice. Additionally, EGF has been shown to inhibit gastric secretion, is involved in wound healing and signals through a receptor known as c-erbB (a class I tyrosine kinase receptor).
Product Specifications
Catalog ID | 1550-04 |
---|---|
MW | ~6.2 kDa |
Storage/Handling | Store desiccated at -20°C. |
EGTA
Catalog ID | Size | Pricing | |
---|---|---|---|
E-217-10 | 10 g | $ 35.00 | |
E-217-25 | 25 g | $ 65.00 | |
E-217-100 | 100 g | $ 129.00 | |
E-217-250 | 250 g | $ 259.00 | |
E-217-500 | 5 x 100 g | $ 385.00 |
Description
Ethyleneglycol bis(2-aminoethyl ether)-N,N,N',N' tetraacetic acid, (EGTA) is a calcium specific chelator. Because of its low affinity for Mg+, EGTA inhibits metalloproteases.
Product Specifications
Catalog ID | E-217 |
---|---|
CAS # | 67-42-5 |
MW | 380.35 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at room temperature. |
EHA105 (pSoup) Agrobacterium Chemically Competent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-118-5x50 | 5 x 50 μL |
|
$ 152.00 |
CC-118-10x50 | 10 x 50 μL |
|
$ 268.00 |
CC-118-15x50 | 15 x 50 μL |
|
$ 360.00 |
Description
GoldBio’s EHA105 (pSoup) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.
The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.
GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥5 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 (pSoup) Agrobacterium ElectroCompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-235-5x50 | 5 x 50 μL |
|
$ 160.00 |
CC-235-10x50 | 10 x 50 μL |
|
$ 288.00 |
CC-235-15x50 | 15 x 50 μL |
|
$ 388.00 |
Description
EHA105 (pSoup) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids, and also helps with Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup)
Transformation efficiency: ≥4 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 (pSoup-P19) Agrobacterium Chemically Competent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-128-5x50 | 5 x 50 μL |
|
$ 152.00 |
CC-128-10x50 | 10 x 50 μL |
|
$ 268.00 |
CC-128-15x50 | 15 x 50 μL |
|
$ 360.00 |
Description
GoldBio’s EHA105 (pSoup-P19) chemically competent Agrobacterium cells are optimized for high transformation efficiencies, making them ideal for applications such as cDNA or gDNA library construction. These cells are resistant to rifampicin, chloramphenicol, and streptomycin, providing versatility in selection and antibiotic resistance EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene.
The pSoup plasmid present in these cells is required for the replication of pGreen, 62SK, and pGs2 series plasmids, increasing the range of applications for the EHA105 (pSoup) system. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These competent cells are suitable for Agrobacterium-mediated transformation of dicots such as Arabidopsis thaliana, tobacco, potato, as well as monocots like corn, making them an ideal choice for plant molecular biology research.
GoldBio’s EHA105 Agrobacteriumstrain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥2 x 103 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 (pSoup-P19) Agrobacterium ElectroCompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-245-5x50 | 5 x 50 μL |
|
$ 160.00 |
CC-245-10x50 | 10 x 50 μL |
|
$ 288.00 |
CC-245-15x50 | 15 x 50 μL |
|
$ 388.00 |
Description
EHA105 (pSoup-P19) ElectroCompetent Agrobacterium cells are designed to provide high transformation efficiencies for applications such as cDNA or gDNA library construction. The strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA), which carries the vir gene necessary for the insertion of T-DNA into the plant genome. Although the pEHA105 plasmid is incapable of transferring its own T-DNA, it can still transfer foreign binary vector T-DNA.
The presence of the pSoup helper plasmid is required for the replication of pGreen, 62SK, and pGs2 series plasmids. The p19 protein is derived from tomato bush dwarf virus and further improves the stability of heterologous gene transcripts by inhibiting RNA silencing of foreign. These features help to enhance the Agrobacterium-mediated transformation of dicotyledonous plants like Arabidopsis thaliana, tobacco, and potato, as well as monocotyledonous plants such as corn.
GoldBio’s EHA105 Agrobacterium strain was generated, and primary clone supplied by Dr. Elizabeth Hood.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105 (pSoup-P19)
Transformation efficiency: ≥3 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 Agrobacterium Chemically Competent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-108-5x50 | 5 x 50 μL |
|
$ 152.00 |
CC-108-10x50 | 10 x 50 μL |
|
$ 268.00 |
CC-108-15x50 | 15 x 50 μL |
|
$ 360.00 |
Description
GoldBio’s EHA105 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Product Specifications
Competent cell type: Chemical Competent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥5 x 104 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
EHA105 Agrobacterium ElectroCompetent Cells
Catalog ID | Size | Pricing | |
---|---|---|---|
CC-225-5x50 | 5 x 50 μL |
|
$ 160.00 |
CC-225-10x50 | 10 x 50 μL |
|
$ 288.00 |
CC-225-15x50 | 15 x 50 μL |
|
$ 388.00 |
Description
GoldBio’s EHA105 ElectroCompetent Agrobacterium cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. Our EHA105 strain contains a rifampicin resistance gene (rif) and an amber basic Ti plasmid pEHA105 (pTiBo542DT-DNA) without self-transport function, containing the vir gene. EHA105 is ideal for genetic transformation of rice, tobacco and several other plants.
Product Specifications
Competent cell type: ElectroCompetent
Species: A. tumefaciens
Strain: EHA105
Transformation efficiency: ≥1.6 x 108 cfu/µg pCAMBIA1391z DNA
Blue/white screening: No
Embelin
Catalog ID | Size | Pricing | |
---|---|---|---|
E-310-10 | 10 mg | $ 88.00 | |
E-310-50 | 50 mg | $ 316.00 |
Description
Embelin is a benzoquinone derivative found in Ardisia japonica which is a plant native to eastern Asia. It is used as an anti-cancer compound as well as an anti-inflammatory. Embelin has also been exhibited anthelminthic properties against cestode parasites.
In oncology research, embelin has been used to disrupt X-linked inhibitor of apoptosis (XIAP) activity. Embelin binds the Baculovirus IAP Repeat 3 (BIR3) domain on XIAP and subsequently disrupts apoptosis regulation. Inactivation of BIR3 allows proteins caspase-9 and Diablo homologs/Smac proteins to freely promote apoptotic pathways.
Embelin has also been used to quell inflammatory responses by inhibiting eicosanoid synthesis.It has been shown that embelin targets eicosanoid synthesizing proteins 5-lipoxygenase (5-LO) and microsomal prostaglandin E2 synthase (MPGES). Embelin has also been shown to disrupt eicosanoid synthesis through XIAP suppression.
Product Specifications
Catalog ID | E-310 |
---|---|
CAS # | 550-24-3 |
MW | 294.38 g/mol |
Emodin
Catalog ID | Size | Pricing | |
---|---|---|---|
E-410-50 | 50 mg | $ 117.00 | |
E-410-250 | 250 mg | $ 401.00 |
Description
Emodin is a compound used as an anti-inflammatory and anti-cancer agent. It has been shown that emodin suppresses inflammatory responses in vitro by inhibiting nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) protein complexes. This effect occurs from emodin binding and preserving an inhibitory subunit on NF-κB.
Emodin has also been shown to induce apoptosis in mouse neuroectodermal tumors both in vivo and in vitro. Cell death is induced by inhibiting protein tyrosine kinase (PTK), a regulator of cell growth. Constraining PTK activity prevents further cell development which signals an apoptotic cascade.
Product Specifications
Catalog ID | E-410 |
---|---|
CAS # | 518-82-1 |
MW | 270.24 g/mol |
Enoxacin
Catalog ID | Size | Pricing | |
---|---|---|---|
E-900-1 | 1 g | $ 80.00 |
Description
Enoxacin is a fluoroquinolone antibiotic compound that is markedly effective against gram negative bacteria. It has been used to study fluoroquinolone cross-resistence in Escehria coli, Pseudomonas aeruginosa and Staphylococcus aureus. Enoxacin is structurally related to nalidixic acid and inhibits DNA gyrase in bacteria.
Fluoroquinolones are a group of broad-spectrum antibiotics that inhibit DNA synthesis. These antibiotics prevent unwinding of double-stranded DNA by binding DNA gyrase or DNA topoisomerase. By preventing the formation of single-stranded DNA, fluoroquinolones inhibit bacterial replication.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
Product Specifications
Catalog ID | E-900 |
---|---|
CAS # | 74011-58-8 |
MW | 347.36 g/mol |
Enramycin
Catalog ID | Size | Pricing | |
---|---|---|---|
E-610-10 | 10 mg | $ 211.00 |
Description
Enramycin is a polypeptide antibiotic that displays strong activity against gram positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). It is produced by the fungus Streptomyces fungicidus as Enramycin A (EA) and Enramycin B (EB), with this product containing an approximate 70:30 ratio of EA to EB. Enramycin has been effective against antibiotic resistant bacteria by inhibiting peptidoglycan synthesis for bacterial cell walls.
Enramycin has been proposed to halt peptidoglycan synthesis by inhibiting transferase enzyme MurG or by inhibiting transglycosylase activity. Both inhibitory pathways entail enramycin binding to peptidoglycan precursors in a cell wall synthesis pathway. Some studies have shown that enramycin binds the precursor Lipid I and inhibits MurG. Enramycin has also been shown to bind the Lipid II and prevent transglycosylation of the precursor by tranglycosylases.
Antibiotics are often used in clinical in vitro tests known as antimicrobial susceptibility tests or ASTs to determine their efficacy against certain bacterial species. They are tested against gram-negative and gram-positive bacteria using panels, discs, and MIC strips by medical microbiologists. ASTs decrease the risk of using an antibiotic against bacteria exhibiting resistance to it, and the results are used in clinical settings to determine which antibiotic(s) to prescribe for various infections.
Product Specifications
Catalog ID | E-610 |
---|---|
CAS # | 11115-82-5 |
Enrofloxacin
Catalog ID | Size | Pricing | |
---|---|---|---|
E-230-5 | 5 g | $ 133.00 | |
E-230-25 | 25 g | $ 541.00 |
Description
Enrofloxacin is a fluoroquinolone antibiotic targeting gram-positive and gram-negative bacteria as well as some Mycoplasma species. It has been useful in researching resistance to quinolones in Salmonella enterica, Mycoplasma gallisepticum, and E. coli.
Product Specifications
Catalog ID | E-230 |
---|---|
CAS # | 93106-60-6 |
MW | 359.39 g/mol |
Grade | MOLECULAR BIOLOGY GRADE |
Storage/Handling | Store at 4°C. |