Enzyme Substrates

Gold Biotechnology offers an extensive amount of enzyme substrates that are ideal for your research needs. These substrates include chromogenic and fluorogenic substrates such as firefly luciferin, MUG, X- α-Gal, X-Gluc, as well as many others. Our superior substrates are ideal for a number of biological applications and are available for a much more affordable price so that your research dollars are focused on the things that matter.
Showing 1-20 of 147 items.

1-O-Methyl-α- D- galactopyranoside (1-O-Methyl-galactoside)

Catalog ID Size Pricing
M-270-50 50 g $ 171.00
M-270-100 100 g $ 321.00
M-270-250 250 g $ 750.00
M-270-500 500 g $ 1,400.00

Description

No description available

Product Specifications

Catalog ID M-270
CAS # 3396-99-4
MW 194.19 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C. Protect from light.
View
Quick Add
M-270-50 50 g $ 171.00
M-270-100 100 g $ 321.00
M-270-250 250 g $ 750.00
M-270-500 500 g $ 1,400.00

1-O-Methyl-α-D-mannopyranoside

Catalog ID Size Pricing
M-485-500 500 g $ 300.00
M-485-1 1 kg $ 566.00
M-485-2.5 2.5 kg $ 1,335.00
M-485-5 5 kg $ 2,519.00

Description

No description available

Product Specifications

Catalog ID M-485
CAS # 617-04-9
MW 194.18 g/mol
Grade MOLECULAR BIOLOGY GRADE
View
Quick Add
M-485-500 500 g $ 300.00
M-485-1 1 kg $ 566.00
M-485-2.5 2.5 kg $ 1,335.00
M-485-5 5 kg $ 2,519.00

1-O-Methyl-β- D- glucuronic acid, sodium salt

Catalog ID Size Pricing
M-310-500 500 mg $ 73.00
M-310-5 5 g $ 286.00
M-310-10 10 g $ 465.00
M-310-25 25 g $ 945.00

Description

1-O-Methyl-β-D-glucuronic acid, sodium salt is an inducer of β-glucuronidase, a glycosidase enzyme coded by the GUS gene.

The GUS gene is the most extensively used reporter gene, particularly in plant biology because it offers the advantage of having no background activity.

β-glucuronidase also has an important role within human digestion because this enzyme is capable of deconjugating toxins within the body and releasing them back freely into the bloodstream. There is a positive relationship between higher levels of β-glucuronidase in the colon and increased cancer risk. However, β-glucuronidase, at certain levels, is necessary, and deficiencies can result in mucopolysaccharidosis type VII or Sly syndrome in newborns.

Product Specifications

Catalog ID M-310
CAS # 134253-42-2
MW 230.00 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C. Protect from light.
View
Quick Add
M-310-500 500 mg $ 73.00
M-310-5 5 g $ 286.00
M-310-10 10 g $ 465.00
M-310-25 25 g $ 945.00

2,3,5-Triphenyltetrazolium chloride (TTC; Tetrazolium chloride)

Catalog ID Size Pricing
T-112-25 25 g $ 47.00
T-112-100 100 g $ 158.00
T-112-250 250 g $ 370.00
T-112-500 500 g $ 691.00

Description

Tetrazolium red is used to visualize dehydrogenase enzyme activity. Initially, the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions, which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.

Product Specifications

Catalog ID T-112
CAS # 298-96-4
MW 334.81 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C. Protect from light.
View
Quick Add
T-112-25 25 g $ 47.00
T-112-100 100 g $ 158.00
T-112-250 250 g $ 370.00
T-112-500 500 g $ 691.00

2-Naphthyl caprylate Octanoic acid (β-naphthyl ester)

Catalog ID Size Pricing
N-100-500 500 mg $ 136.00
N-100-1 1 g $ 204.00
N-100-2.5 2.5 g $ 446.00
N-100-5 5 g $ 775.00

Description

2-Naphthyl caprylate is a chromogenic substrate for carboxylesterase and lipase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a diazonium salt, the corresponding azo-dye is formed.

Naphthols can also be detected by fluorescence analysis, and can be used in a colorimetric assay for the extracellular lipase of Pseudomonas fluorescent B52.

Product Specifications

Catalog ID N-100
CAS # 10251-17-9
MW 270.37 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
N-100-500 500 mg $ 136.00
N-100-1 1 g $ 204.00
N-100-2.5 2.5 g $ 446.00
N-100-5 5 g $ 775.00

2-Naphthyl-2-Naphthyl-β- D-galactopyranoside (β-Naphthyl-β-galactoside)

Catalog ID Size Pricing
N-120-1 1 g $ 70.00
N-120-2.5 2.5 g $ 83.00
N-120-10 10 g $ 221.00
N-120-50 50 g $ 1,024.00

Description

No description available

Product Specifications

Catalog ID N-120
CAS # 33993-25-8
MW 306.32 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
N-120-1 1 g $ 70.00
N-120-2.5 2.5 g $ 83.00
N-120-10 10 g $ 221.00
N-120-50 50 g $ 1,024.00

2-Naphthyl-α- L-fucopyranoside (β-Naphthyl-α-L-fucoside)

Catalog ID Size Pricing
N-110-500 500 mg $ 274.00
N-110-1 1 g $ 503.00
N-110-2.5 2.5 g $ 1,154.00
N-110-5 5 g $ 2,118.00

Description

No description available

Product Specifications

Catalog ID N-110
CAS # 63503-05-9
MW 290.32 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
N-110-500 500 mg $ 274.00
N-110-1 1 g $ 503.00
N-110-2.5 2.5 g $ 1,154.00
N-110-5 5 g $ 2,118.00

2-Naphthyl-α-D-glucopyranoside

Catalog ID Size Pricing
N-125-500 500 mg $ 121.00
N-125-1 1 g $ 210.00
N-125-2.5 2.5 g $ 469.00

Description

No description available

Product Specifications

Catalog ID N-125
CAS # 25320-79-0
MW 306.32 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C.
View
Quick Add
N-125-500 500 mg $ 121.00
N-125-1 1 g $ 210.00
N-125-2.5 2.5 g $ 469.00

2-Nitrophenyl-1-thio-β-D-galactopyranoside

Catalog ID Size Pricing
N-390-500 500 mg $ 218.00
N-390-1 1 g $ 433.00
N-390-2.5 2.5 g $ 1,072.00
N-390-5 5 g $ 2,122.00

Description

No description available

Product Specifications

Catalog ID N-390
CAS # 1158-17-4
MW 317.32 g/mol
Grade MOLECULAR BIOLOGY GRADE
View
Quick Add
N-390-500 500 mg $ 218.00
N-390-1 1 g $ 433.00
N-390-2.5 2.5 g $ 1,072.00
N-390-5 5 g $ 2,122.00

2-Nitrophenyl-N-acetyl-β-D-glucosaminide

Catalog ID Size Pricing
N-210-500 500 mg $ 147.00
N-210-5 5 g $ 215.00
N-210-10 10 g $ 409.00
N-210-25 25 g $ 972.00

Description

No description available

Product Specifications

Catalog ID N-210
CAS # 13264-92-1
MW 342.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
View
Quick Add
N-210-500 500 mg $ 147.00
N-210-5 5 g $ 215.00
N-210-10 10 g $ 409.00
N-210-25 25 g $ 972.00

3-Indoxyl phosphate bis (2-amino-2-methyl-1,3-propanediol) salt

Catalog ID Size Pricing
I-490-50 50 mg $ 63.00
I-490-500 500 mg $ 187.00
I-490-1 1 g $ 288.00
I-490-2.5 2.5 g $ 481.00

Description

No description available

Product Specifications

Catalog ID I-490
CAS # 107475-12-7
MW 423.41 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
View
Quick Add
I-490-50 50 mg $ 63.00
I-490-500 500 mg $ 187.00
I-490-1 1 g $ 288.00
I-490-2.5 2.5 g $ 481.00

4-Aminophenyl phosphate monosodium salt hydrate

Catalog ID Size Pricing
A-292-100 100 mg $ 63.00
A-292-500 500 mg $ 126.00
A-292-1 1 g $ 234.00
A-292-2.5 2.5 g $ 517.00

Description

P-aminophenyl phosphate (pAPP) can be used as a substrate for alkaline phosphatase enzymatic reactions in order to electrochemically quantify ALP activity.

The enzymatic reaction results in the production of phenol, which can be quantified and used to analyze ALP concentrations.

ALP is a naturally occurring enzyme and can be found in human tissue in the liver, bone and intestines. Usual concentrations of ALP range between 25-100 IU/L. A significant spike outside of that range could act as a signal for many diseases.

Product Specifications

Catalog ID A-292
CAS # 108084-47-5
MW 211.90 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
A-292-100 100 mg $ 63.00
A-292-500 500 mg $ 126.00
A-292-1 1 g $ 234.00
A-292-2.5 2.5 g $ 517.00

4-Aminophenyl-β-D-galactopyranoside

Catalog ID Size Pricing
A-280-500 500 mg $ 94.00
A-280-1 1 g $ 159.00
A-280-2.5 2.5 g $ 362.00
A-280-5 5 g $ 658.00

Description

No description available

Product Specifications

Catalog ID A-280
CAS # 5094-33-7
MW 271.27 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
View
Quick Add
A-280-500 500 mg $ 94.00
A-280-1 1 g $ 159.00
A-280-2.5 2.5 g $ 362.00
A-280-5 5 g $ 658.00

4-Chloro-1-naphthol

Catalog ID Size Pricing
C-273-50 50 g $ 103.00
C-273-100 100 g $ 180.00
C-273-250 250 g $ 383.00
C-273-500 500 g $ 716.00

Description

No description available

Product Specifications

Catalog ID C-273
CAS # 604-44-4
MW 178.62 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
C-273-50 50 g $ 103.00
C-273-100 100 g $ 180.00
C-273-250 250 g $ 383.00
C-273-500 500 g $ 716.00

4-Methylumbelliferyl caprylate

Catalog ID Size Pricing
M-540-1 1 g $ 121.00
M-540-2.5 2.5 g $ 273.00
M-540-5 5 g $ 530.00
M-540-10 10 g $ 990.00

Description

No description available

Product Specifications

Catalog ID M-540
CAS # 20671-66-3
MW 302.37 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
M-540-1 1 g $ 121.00
M-540-2.5 2.5 g $ 273.00
M-540-5 5 g $ 530.00
M-540-10 10 g $ 990.00

4-Methylumbelliferyl palmitate

Catalog ID Size Pricing
M-690-250 250 mg $ 64.00
M-690-500 500 mg $ 92.00
M-690-1 1 g $ 141.00
M-690-2.5 2.5 g $ 265.00

Description

4-Methylumbelliferyl palmitate is used as a fluorogenic substrate for a subclass of esterases called lipase.

Lipase is an enzyme that assists in the hydrolysis of fats. They have a large role in digestion as well as dietary lipid transport.

Lysosomal acid lipase (LAL), in particular, is required for the hydrolysis of cholesterol esters which are then delivered into the lysosome. Deficiencies in LAL are primarily associated with two diseases: cholesteryl ester storage disease (CESD) and Wolman’s disease (WD). 4-Methylumbelliferyl palmitate has often been used as one of the lipase substrates in studies involving either of these diseases.

Product Specifications

Catalog ID M-690
CAS # 17695-48-6
MW 414.58 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
M-690-250 250 mg $ 64.00
M-690-500 500 mg $ 92.00
M-690-1 1 g $ 141.00
M-690-2.5 2.5 g $ 265.00

4-Methylumbelliferyl phosphate bis (2-amino- 2-methyl-1,3-propanediol) salt

Catalog ID Size Pricing
M-710-2.5 2.5 g $ 185.00
M-710-5 5 g $ 321.00
M-710-10 10 g $ 558.00
M-710-25 25 g $ 1,213.00

Description

No description available

Product Specifications

Catalog ID M-710
CAS # 107475-10-5
MW 466.42 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
M-710-2.5 2.5 g $ 185.00
M-710-5 5 g $ 321.00
M-710-10 10 g $ 558.00
M-710-25 25 g $ 1,213.00

4-Methylumbelliferyl phosphate bis (cyclohexylammonium) salt

Catalog ID Size Pricing
M-720-10 10 g $ 265.00
M-720-25 25 g $ 603.00
M-720-50 50 g $ 1,095.00
M-720-100 100 g $ 1,990.00

Description

No description available

Product Specifications

Catalog ID M-720
CAS # 128218-53-1
MW 454.50 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
M-720-10 10 g $ 265.00
M-720-25 25 g $ 603.00
M-720-50 50 g $ 1,095.00
M-720-100 100 g $ 1,990.00

4-Methylumbelliferyl phosphate, free acid

Catalog ID Size Pricing
M-700-250 250 mg $ 34.00
M-700-10 10 g $ 201.00
M-700-25 25 g $ 476.00
M-700-50 50 g $ 905.00

Description

4-Methylumbelliferyl phosphate (MUP) is a sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatase.

Phosphotase activity has long been studied due to its importance in cellular function. Upon dephosphorylation, MUP yields fluorescent 4-methylumelliferone (4-MU). This reaction has been used to spot PCR amplified products in ELISA and to classify bacteria.

More specifically, MUP can act as an indicator of C. perfringens because of its ability to detect phosphatase which is a highly specific indicator of C. perfringens.

Product Specifications

Catalog ID M-700
CAS # 3368-04-5
MW 256.15 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
View
Quick Add
M-700-250 250 mg $ 34.00
M-700-10 10 g $ 201.00
M-700-25 25 g $ 476.00
M-700-50 50 g $ 905.00

4-Methylumbelliferyl sulfate, potassium salt

Catalog ID Size Pricing
M-760-5 5 g $ 272.00
M-760-10 10 g $ 519.00
M-760-25 25 g $ 1,234.00
M-760-50 50 g $ 2,347.00

Description

No description available

Product Specifications

Catalog ID M-760
CAS # 15220-11-8
MW 294.33 g/mol
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
M-760-5 5 g $ 272.00
M-760-10 10 g $ 519.00
M-760-25 25 g $ 1,234.00
M-760-50 50 g $ 2,347.00

Join our list to receive promos and articles.

Join Now