Featured Products

Our most popular products from our most popular categories, from our Proven and PublishedTM Luciferin to our popular antibiotic lines and electrophoresis tools. GoldBio’s commitment to quality and affordability makes these products our top sellers.
Showing 1-20 of 24 items.

1 kb DNA Ladder

1 kb DNA Ladder
Catalog ID Size Pricing
D010-500 500 µL $ 46.00
D010-1000 2x500 µL $ 86.00
D010-1500 3x500 µL $ 121.00
D010-2000 4x500 µL $ 161.00
D010-2500 5x500 µL $ 184.00
D010-3000 6x500 µL $ 201.00

Description

A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D010
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D010-500 500 µL $ 46.00
D010-1000 2x500 µL $ 86.00
D010-1500 3x500 µL $ 121.00
D010-2000 4x500 µL $ 161.00
D010-2500 5x500 µL $ 184.00
D010-3000 6x500 µL $ 201.00

100 bp DNA Ladder

Catalog ID Size Pricing
D001-500 500 µL $ 46.00
D001-1000 2x500 µL $ 86.00
D001-1500 3x500 µL $ 121.00
D001-2000 4x500 µL $ 161.00
D001-2500 5x500 µL $ 184.00
D001-3000 6x500 µL $ 201.00

Description

A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size. The ladder is ready-to-use, and is premixed with loading buffer dye for direct loading on gel.

Product Specifications

Catalog ID D001
Storage/Handling Store at room temperature for 6 months. Store at -20°C for 24 months.
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D001-500 500 µL $ 46.00
D001-1000 2x500 µL $ 86.00
D001-1500 3x500 µL $ 121.00
D001-2000 4x500 µL $ 161.00
D001-2500 5x500 µL $ 184.00
D001-3000 6x500 µL $ 201.00

Agarose LE (Molecular Biology Grade)

Catalog ID Size Pricing
A-201-100 100 g $ 92.00   $ 79.00
A-201-500 500 g $ 338.00   $ 288.00
A-201-1000 1 kg $ 562.00   $ 478.00

Description

Agarose LE (Low Electroendosmosis) is Molecular Biology Grade and is specifically designed for the superior separation of nucleic acids, with maximally crisp band resolution, and is also ideal for cloning and cloning related experiments.

GoldBio Agarose LE is refined in an advanced process that excludes the use of organic solvents. The result is a cleaner end-product with significantly reduced environmental impact. The agarose can be used for analyses of nucleic acids from 50 bp to 25 kbp, protein electrophoresis and various blotting protocols.

The low EEO of the agarose promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO reduces band distortion (caused by counterflow) that can result from the presence of excessive sulfate-rich negative ions.

Agarose is a natural product that forms an inert matrix used in electrophoresis, chromatography and other molecular biology and biochemistry techniques. Likewise, it is neutral and easily derivatizable, so it is easy to bind to its structure proteins like enzymes, antigens or antibodies. Toxicity absence makes working with agarose very convenient.

Product Specifications

Catalog ID A-201
CAS # 9012-36-6
Storage/Handling Store at room temperature.
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A-201-100 100 g $ 92.00
$ 79.00
A-201-500 500 g $ 338.00
$ 288.00
A-201-1000 1 kg $ 562.00
$ 478.00

Ampicillin (Sodium), USP Grade

Catalog ID Size Pricing
A-301-5 5 g $ 23.00
A-301-10 10 g $ 34.00
A-301-25 25 g $ 46.00
A-301-100 100 g $ 172.00
A-301-500 500 g $ 822.00

Description

Ampicillin sodium is a member of the extended-spectrum β-lactam family and similar in structure to penicillin. It is used in the selection of antibiotic cell hybrids and has a high rate of action against many gram-negative bacterial strains. Ampicillin sodium targets non-ESBL (Extended Spectrum β-lactamase) bacteria including Staphylococcus and Streptococcus species and medically important enteric pathogens such as Shigella and Salmonella. It has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus species. Resistance to ampicillin is routinely utilized as a selectable marker to confirm successful cell transformation. Ampicillin Sodium is freely soluble in water.

Product Specifications

Catalog ID A-301
CAS # 69-52-3
MW 371.39 g/mol
Storage/Handling Store desiccated at -20°C.
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A-301-5 5 g $ 23.00
A-301-10 10 g $ 34.00
A-301-25 25 g $ 46.00
A-301-100 100 g $ 172.00
A-301-500 500 g $ 822.00

BLUEstain™ 2 Protein ladder, 5-245 kDa

Catalog ID Size Pricing
P008-500 500 µL $ 115.00
P008-1000 2x500 µL $ 224.00
P008-1500 3x500 µL $ 321.00
P008-2000 4x500 µL $ 417.00
P008-2500 5x500 µL $ 506.00
P008-3000 6x500 µL $ 591.00

Description

The BLUEstain™ 2 Protein ladder is a three-color protein standard with 13 prestained proteins, covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). 


The BLUEstain™ 2 Protein ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute or add reducing agent before loading.

Product Specifications

Catalog ID P008
Storage/Handling Store at 4°C for 3 months. Store at -20°C for 24 months.
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P008-500 500 µL $ 115.00
P008-1000 2x500 µL $ 224.00
P008-1500 3x500 µL $ 321.00
P008-2000 4x500 µL $ 417.00
P008-2500 5x500 µL $ 506.00
P008-3000 6x500 µL $ 591.00

BLUEstain™ Protein ladder, 11-245 kDa

Catalog ID Size Pricing
P007-500 500 µL $ 115.00
P007-1000 2x500 µL $ 224.00
P007-1500 3x500 µL $ 321.00
P007-2000 4x500 µL $ 417.00
P007-2500 5x500 µL $ 506.00
P007-3000 6x500 µL $ 591.00

Description

The BLUEstain™ Protein ladder is a three-color protein standard with 12 prestained proteins covering a wide range molecular weights from ~11 to 245 kDa. Proteins are covalently coupled with a bluechromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUEstain™ Protein ladder, is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.

Product Specifications

Catalog ID P007
Storage/Handling Store at 4°C for 3 months. Store at -20°C for 24 months.
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P007-500 500 µL $ 115.00
P007-1000 2x500 µL $ 224.00
P007-1500 3x500 µL $ 321.00
P007-2000 4x500 µL $ 417.00
P007-2500 5x500 µL $ 506.00
P007-3000 6x500 µL $ 591.00

Carbenicillin (Disodium)

Catalog ID Size Pricing
C-103-5 5 g $ 46.00
C-103-25 25 g $ 150.00
C-103-50 50 g $ 264.00
C-103-100 100 g $ 450.00
C-103-250 250 g $ 911.00

Description

Carbenicillin is a member of the penicillin β-lactam antibiotics. However, unlike most β-lactams, carbenicillin disodium is limited to primarily gram-negative bacteria including Pseudomonas aeruginosa and common enteric species. It can be used in selection protocols and is often preferred over ampicillin due to its increased stability. This allows for less contamination of unwanted bacterial colonies. In addition, carbenicillin has been used to regulate bacterial growth in plants, yielding low regeneration frequencies. It can be used in genetic transformation applications to select for AmpR transformed cells.

Product Specifications

Catalog ID C-103
CAS # 4800-94-6
MW 422.36 g/mol
Grade MOLECULAR BIOLOGY GRADE
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C-103-5 5 g $ 46.00
C-103-25 25 g $ 150.00
C-103-50 50 g $ 264.00
C-103-100 100 g $ 450.00
C-103-250 250 g $ 911.00

Chloramphenicol, USP Grade

Catalog ID Size Pricing
C-105-5 5 g $ 12.00
C-105-25 25 g $ 23.00
C-105-100 100 g $ 79.00

Description

Chloramphenicol, also known as chlornitromycin, is a broad-spectrum, bacteriostatic antibiotic derived from Streptomyces venezuelae. It is effective against several gram-positive and gram-negative bacteria and commonly used in researching protein synthesis and to select for chloramphenicol-resistant transformed cells or the bacterial CAT gene.

Product Specifications

Catalog ID C-105
CAS # 56-75-7
MW 323.13 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
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C-105-5 5 g $ 12.00
C-105-25 25 g $ 23.00
C-105-100 100 g $ 79.00

D-Luciferin, Potassium Salt (Proven and Published™)

Catalog ID Size Pricing
LUCK-100 100 mg $ 52.00
LUCK-250 250 mg $ 125.00
LUCK-300 300 mg $ 146.00
LUCK-500 500 mg $ 188.00
LUCK-1G 1 g $ 245.00
LUCK-2G 2 x 1 g $ 472.00
LUCK-3G 3 x 1 g $ 702.00
LUCK-4G 4 x 1 g $ 928.00
LUCK-5G 5 x 1 g $ 1,150.00
LUCK-10G 10 x 1 g $ 2,280.00

Description

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.

Firefly Luciferin is identical to Beetle Luciferin. (See "Additional Information" for structures.)

Product Specifications

Catalog ID LUCK
CAS # 115144-35-9
MW 318.42 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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LUCK-100 100 mg $ 52.00
LUCK-250 250 mg $ 125.00
LUCK-300 300 mg $ 146.00
LUCK-500 500 mg $ 188.00
LUCK-1G 1 g $ 245.00
LUCK-2G 2 x 1 g $ 472.00
LUCK-3G 3 x 1 g $ 702.00
LUCK-4G 4 x 1 g $ 928.00
LUCK-5G 5 x 1 g $ 1,150.00
LUCK-10G 10 x 1 g $ 2,280.00

D-Luciferin, Sodium Salt (Proven and Published™)

Catalog ID Size Pricing
LUCNA-100 100 mg $ 52.00
LUCNA-250 250 mg $ 125.00
LUCNA-300 300 mg $ 146.00
LUCNA-500 500 mg $ 188.00
LUCNA-1G 1 g $ 245.00
LUCNA-2G 2 x 1 g $ 472.00
LUCNA-3G 3 x 1 g $ 702.00
LUCNA-4G 4 x 1 g $ 928.00
LUCNA-5G 5 x 1 g $ 1,150.00
LUCNA-10G 10 x 1 g $ 2,280.00

Description

Tested and Certified for in vivo imaging (See "Luciferin FAQ" in additional information)

Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg2+ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.

Firefly Luciferin is identical to Beetle Luciferin. (See "Additional Information" for structures.)

Product Specifications

Catalog ID LUCNA
CAS # 103404-75-7
MW 320.32 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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LUCNA-100 100 mg $ 52.00
LUCNA-250 250 mg $ 125.00
LUCNA-300 300 mg $ 146.00
LUCNA-500 500 mg $ 188.00
LUCNA-1G 1 g $ 245.00
LUCNA-2G 2 x 1 g $ 472.00
LUCNA-3G 3 x 1 g $ 702.00
LUCNA-4G 4 x 1 g $ 928.00
LUCNA-5G 5 x 1 g $ 1,150.00
LUCNA-10G 10 x 1 g $ 2,280.00

DTT (Dithiothreitol) (> 99% pure) Protease free

Catalog ID Size Pricing
DTT10 10 g $ 49.00
DTT25 25 g $ 109.00
DTT50 50 g $ 198.00
DTT100 100 g $ 319.00
DTT500 500 g $ 1,425.00

Description

Dithiothreitol (DTT) is the common name of the more popular of the two Cleland’s Reagents (the other being Dithioerythritol or DTE). DTT is a powerful reducing agent that forms a stable six-membered ring with an internal disulfide bond which is resistant to oxidation. DTT is often used for the following: reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl) cystamine to break apart the matrix of a polyacrylamide gel, the reduction of disulfide bonds in proteins, the prevention of those bonds from forming between cysteine residues and lastly, DTT is often used to reduce thiolated DNA in order to minimize dimerization.

DTT is a nearly 7-fold stronger reduction agent than βME (β-mercaptoethanol) and has a less offensive odor and is less toxic.

Product Specifications

Catalog ID DTT
CAS # 27565-41-9 / 3483-12-3
MW 154.25 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
UN Number 3335
Item Class 9
Group Number III
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DTT10 10 g $ 49.00
DTT25 25 g $ 109.00
DTT50 50 g $ 198.00
DTT100 100 g $ 319.00
DTT500 500 g $ 1,425.00

IPTG

Catalog ID Size Pricing
I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00
I2481C25 25 g $ 115.00
I2481C50 50 g $ 184.00
I2481C100 100 g $ 349.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

Product Specifications

Catalog ID I2481
CAS # 367-93-1
MW 238.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00
I2481C25 25 g $ 115.00
I2481C50 50 g $ 184.00
I2481C100 100 g $ 349.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Kanamycin Monosulfate, USP Grade

Catalog ID Size Pricing
K-120-5 5 g $ 23.00
K-120-10 10 g $ 44.00
K-120-25 25 g $ 92.00
K-120-50 50 g $ 173.00
K-120-100 100 g $ 323.00
K-120-250 250 g $ 748.00

Description

Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus. It is effective against gram-positive and gram-negative bacteria as well as Mycoplasma species. Kanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have the NPT II (APH3) gene incorporated in them. Kanamycin is very soluble in aqueous solution and is typically used in concentrations of 50-100 µg/ml.

Product Specifications

Catalog ID K-120
CAS # 25389-94-0
MW 582.58 g/mol
Storage/Handling Store desiccated at -20°C.
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K-120-5 5 g $ 23.00
K-120-10 10 g $ 44.00
K-120-25 25 g $ 92.00
K-120-50 50 g $ 173.00
K-120-100 100 g $ 323.00
K-120-250 250 g $ 748.00

Nourseothricin Sulfate (Streptothricin Sulfate) (NTC or clonNAT)

Catalog ID Size Pricing
N-500-100 100 mg $ 73.00
N-500-500 500 mg $ 191.00
N-500-1 1 g $ 273.00
N-500-2 2 x 1 g $ 501.00
N-500-3 3 x 1 g $ 719.00
N-500-4 4 x 1 g $ 916.00
N-500-5 5 x 1 g $ 1,091.00

Description

Nourseothricin is a broad-spectrum antibiotic derived from Streptomyces noursei. In addition to being effective on prokaryotic cells of gram-positive and gram-negative bacteria, various fungi including Candida albicans, and certain DNA and RNA viruses, it is also effective on eukaryotic cells of higher eukaryotes. It has been used as a selection agent for cells containing the gene conferring resistance to it.

Product Specifications

Catalog ID N-500
CAS # 96736-11-7
MW 1359.47 g/mol
Storage/Handling Store desiccated at 4°C.
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N-500-100 100 mg $ 73.00
N-500-500 500 mg $ 191.00
N-500-1 1 g $ 273.00
N-500-2 2 x 1 g $ 501.00
N-500-3 3 x 1 g $ 719.00
N-500-4 4 x 1 g $ 916.00
N-500-5 5 x 1 g $ 1,091.00

TCEP-HCl

Catalog ID Size Pricing
TCEP1 1 g $ 19.00
TCEP2 2 g $ 34.00
TCEP5 5 g $ 68.00
TCEP10 10 g $ 110.00
TCEP25 25 g $ 229.00
TCEP50 50 g $ 427.00
TCEP100 100 g $ 844.00

Description

TCEP-HCl is an odorless reducing agent suitable for protein applications and in the preparation for electrophoresis. TCEP is water soluble, is stable in a variety of solutions and is particularly useful in the labeling of cysteine residues with maleimides, preventing the cysteines from forming disulfide bonds. As a reducing agent, TCEP has many other desirable features.

Product Specifications

Catalog ID TCEP
CAS # 51805-45-9
MW 286.65 g/mol
Storage/Handling Store desiccated at -20°C.
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TCEP1 1 g $ 19.00
TCEP2 2 g $ 34.00
TCEP5 5 g $ 68.00
TCEP10 10 g $ 110.00
TCEP25 25 g $ 229.00
TCEP50 50 g $ 427.00
TCEP100 100 g $ 844.00

Vancomycin Hydrochloride, USP Grade

Catalog ID Size Pricing
V-200-1 1 g $ 50.00
V-200-5 5 g $ 115.00
V-200-25 25 g $ 281.00

Description

Vancomycin hydrochloride is a member of the glycopeptide antibiotic class derived from Streptomyces orientalis. It is effective against gram-positive bacteria and used in studies of antibiotic resistance bacteria and nanoparticle transport studies. Exhibiting low toxicity to plants, vancomycin is suitable for plant selection and prevention of bacterial contamination in plant cell culture applications.

Product Specifications

Catalog ID V-200
CAS # 1404-93-9
MW 1485.71 g/mol
Storage/Handling Store desiccated at -20°C.
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V-200-1 1 g $ 50.00
V-200-5 5 g $ 115.00
V-200-25 25 g $ 281.00

X-Gal

Catalog ID Size Pricing
X4281C 1 g $ 42.00
X4281C10 10 g $ 229.00

Description

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside is most commonly known as X-Gal. X-gal is a histochemical substrate for β-galactosidase which cleaves X-Gal to yield an insoluble blue precipitate.

X-Gal is a mainstay in molecular biology cloning applications in which it is used to detect the activity of β-galactosidase. X-Gal is used to detect the insertion of foreign DNA into the lacZ region of a plasmid DNA. Insertion of DNA into the lacZ region results in the loss of β-galactosidase activity. Bacteria cells that retain active β-galactosidase will result in characteristic blue colonies. Successful disruption of the lacZα gene disrupts the α-complementation of the β-galactosidase gene and the precipitate does not form, resulting in white colonies.

Many other applications also use X-Gal as a substrate to detect β-galactosidase activity. These include β-galactosidase -antibody linked immunoassays and immunohistochemistry, coliphage detection based on β-galactosidase induction and the detection of micrometastasis formation during tumor progession.

Product Specifications

Catalog ID X4281
CAS # 7240-90-6
MW 408.63 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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X4281C 1 g $ 42.00
X4281C10 10 g $ 229.00

Hygromycin B

Catalog ID Size Pricing
H-270-1 1 g $ 68.00
H-270-2-1 2 x 1 g $ 127.00
H-270-2.5 2.5 g $ 161.00
H-270-5 5 g $ 281.00
H-270-5-1 5 x 1 g $ 305.00
H-270-10 10 g $ 506.00
H-270-10-1 10 x 1 g $ 518.00

Description

Hygromycin B is an aminoglycoside antibiotic isolated from Streptomyces hygroscopicus. It is commonly used to study protein synthesis. Aside from its efficacy against bacteria, fungi, and higher eukaryotic cells, hygromycin B is also an antiviral agent inhibiting translation upon selectively entering cells made permeable due to viral infection. It works as a standard selection antibiotic in gene experiments, especially for selection of hygromycin resistance gene transformed cells.

Product Specifications

Catalog ID H-270
CAS # 31282-04-9
MW 527.52 g/mol
Storage/Handling Store desiccated at -20°C.
UN Number 3462
Item Class 6.1
Group Number I
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H-270-1 1 g $ 68.00
H-270-2-1 2 x 1 g $ 127.00
H-270-2.5 2.5 g $ 161.00
H-270-5 5 g $ 281.00
H-270-5-1 5 x 1 g $ 305.00
H-270-10 10 g $ 506.00
H-270-10-1 10 x 1 g $ 518.00

G-418 Sulfate

Catalog ID Size Pricing
G-418-1 1 g $ 30.00
G-418-5 5 g $ 137.00
G-418-10 10 g $ 229.00
G-418-25 25 g $ 338.00

Description

G-418 sulfate, also known as geneticin, is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is commonly used in plant and mammalian cell culture as a selectable marker. It can be used to select for cells transformed with an iNOS promoter construct or neomycin resistance gene.

G-418 functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation. Resistance to G-418 is conferred through the nptll gene in plant cells and the neo gene in mammalian cells, which encode aminoglycoside 3'-phosphotransferase. For gene selection applications, a kill curve can be made to determine the minimum effective G418 concentration to kill non-resistant cells. G418 disulfate is freely soluble in water.

Product Specifications

Catalog ID G-418
CAS # 108321-42-2
MW 692.71 g/mol
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G-418-1 1 g $ 30.00
G-418-5 5 g $ 137.00
G-418-10 10 g $ 229.00
G-418-25 25 g $ 338.00

HEPES, Free Acid

Catalog ID Size Pricing
H-400-100 100 g $ 34.00
H-400-250 250 g $ 68.00
H-400-500 500 g $ 103.00
H-400-1 1 kg $ 161.00
H-400-2 2 x 1 kg $ 268.00
H-400-5 5 kg $ 568.00

Description

HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al. It is a zwitterionic, piperazinic buffer that is useful for a pH range of 6.8 – 8.2. HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions.

Product Specifications

Catalog ID H-400
CAS # 7365-45-9
MW 238.30 g/mol
Grade ULTRA PURE GRADE
Storage/Handling Store at room temperature.
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H-400-100 100 g $ 34.00
H-400-250 250 g $ 68.00
H-400-500 500 g $ 103.00
H-400-1 1 kg $ 161.00
H-400-2 2 x 1 kg $ 268.00
H-400-5 5 kg $ 568.00

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