His-tag Purification

His-tag purification is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and chemistry that need to be separated. GoldBio's IDA cross-linked agarose resins consist of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. These resins are loaded with a divalent metal ions (such as nickel, cobalt, zinc or copper) in either high density or low density affinities to meet your specific research requirements. Copper ions have high affinity (binding strength) but low specificity (selectivity); cobalt ions have low affinity but have high specificity; while nickel and zinc combine a mid-ranged affinity and specificity for most general purposes.
Showing 1-19 of 19 items.

5X Phosphate Buffer + 3M Imidazole Solution

Catalog ID Size Pricing
I-905-100 100 mL $ 178.00

Description

This Buffer Set consists of:

- 5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl)
- 25 mL 3M Recrystallized Imidazole Solution, pH 6.0

This Buffer set allows researchers to develop, wash and elute buffers for His-tag protein purification.

Product Specifications

Catalog ID I-905
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I-905-100 100 mL $ 178.00

5X Phosphate Buffer, pH 8

Catalog ID Size Pricing
P-500-50 50 mL $ 58.00
P-500-100 100 mL $ 81.00
P-500-250 250 mL $ 115.00

Description

5X Phosphate Buffer, pH8 (250 mM Phosphate buffer pH 8, 1.5 M NaCl).

Phosphate Buffer, pH8 is a mix of Sodium Phosphate dibasic (Na2HPO4) and Monosodium Phosphate (NaH2PO4). This buffer is the basis for wash and elution buffers for protein purification through His-tag.

Product Specifications

Catalog ID P-500
Storage/Handling Store at 4°C.
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P-500-50 50 mL $ 58.00
P-500-100 100 mL $ 81.00
P-500-250 250 mL $ 115.00

Cobalt Agarose Beads (High Density)

Catalog ID Size Pricing
H-310-5 5 mL $ 45.00
H-310-10 10 mL $ 79.00
H-310-C8 Column: 8 x 1 mL $ 157.00
H-310-25 25 mL $ 179.00
H-310-50 50 mL $ 298.00
H-310-C5 Column: 5 x 5 mL $ 466.00
H-310-100 100 mL $ 546.00
H-310-500 500 mL $ 2,184.00

Description

Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.

Product Specifications

Catalog ID H-310
Storage/Handling store at 4°C. Do NOT freeze.
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H-310-5 5 mL $ 45.00
H-310-10 10 mL $ 79.00
H-310-C8 Column: 8 x 1 mL $ 157.00
H-310-25 25 mL $ 179.00
H-310-50 50 mL $ 298.00
H-310-C5 Column: 5 x 5 mL $ 466.00
H-310-100 100 mL $ 546.00
H-310-500 500 mL $ 2,184.00

Cobalt HTC Agarose Beads

Catalog ID Size Pricing
R-203-5 5 mL $ 64.00
R-203-25 25 mL $ 272.00
R-203-100 100 mL $ 685.00
R-203-500 500 mL $ 2,788.00

Description

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

Product Specifications

Catalog ID R-203
Storage/Handling store at 4°C. Do NOT freeze.
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R-203-5 5 mL $ 64.00
R-203-25 25 mL $ 272.00
R-203-100 100 mL $ 685.00
R-203-500 500 mL $ 2,788.00

Copper Agarose Beads (Low Density)

Catalog ID Size Pricing
H-311-25 25 mL $ 218.00
H-311-100 100 mL $ 577.00

Description

Low Density Copper Agarose Resin is available in bulk or in prepacked columns. Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Cu2+. The resulting resin (ready-to-use) is ideal for rapid purification of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-311
Storage/Handling store at 4°C. Do NOT freeze.
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H-311-25 25 mL $ 218.00
H-311-100 100 mL $ 577.00

His-Tag Buffer Set

Catalog ID Size Pricing
I-906-250 1 kit $ 114.00

Description

His-Tag Buffer Set includes:

- 250 mL Wash Buffer (50mM Phosphate Buffer pH 8, 300mM NaCl, 10mM Imidazole)
- 50 mL Elution Buffer (50mM Phosphate Buffer, 300mM NaCl, 500mM Imidazole)

Designed for use with Goldbio Nickel and Cobalt Agarose resins, but it can be used with most standard His-tag purification resins.

Product Specifications

Catalog ID I-906
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I-906-250 1 kit $ 114.00

Imidazole - 3M Recrystallized Solution, pH 6.0

Catalog ID Size Pricing
I-901-25 25 mL $ 68.00
I-901-50 50 mL $ 101.00
I-901-100 100 mL $ 156.00
I-901-250 250 mL $ 361.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-901
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at 4°C.
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I-901-25 25 mL $ 68.00
I-901-50 50 mL $ 101.00
I-901-100 100 mL $ 156.00
I-901-250 250 mL $ 361.00

Imidazole, Recrystallized

Catalog ID Size Pricing
I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-902
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at room temperature.
UN Number 3262
Item Class 8
Group Number III
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I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

Metal Free Agarose Beads (High Density)

Catalog ID Size Pricing
H-300-25 25 mL $ 218.00
H-300-100 100 mL $ 577.00

Description

Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

Affinity Chromatography is the purification technique that is widely used. It is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin needs to be loaded with a divalent metal of your choosing (Ni2+, Cu2+, Zn2+ or Co2+) or can be used as a negative control to detect non-specific binding. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-300
Storage/Handling store at 4°C. Do NOT freeze.
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H-300-25 25 mL $ 218.00
H-300-100 100 mL $ 577.00

Metal Free Agarose Beads (Low Density)

Catalog ID Size Pricing
H-301-25 25 mL $ 218.00
H-301-100 100 mL $ 577.00

Description

Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

Affinity Chromatography is the purification technique that is widely used. It is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin needs to be loaded with a divalent metal of your choosing (Ni2+, Cu2+, Zn2+ or Co2+) or can be used as a negative control to detect non-specific binding. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-301
Storage/Handling store at 4°C. Do NOT freeze.
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H-301-25 25 mL $ 218.00
H-301-100 100 mL $ 577.00

Nickel Agarose Beads (High Density)

Catalog ID Size Pricing
H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-C5 Column: 5x5 mL $ 466.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00

Description

Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-320
Storage/Handling store at 4°C. Do NOT freeze.
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H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-C5 Column: 5x5 mL $ 466.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00

Nickel Agarose Beads (Low Density)

Catalog ID Size Pricing
H-321-25 25 mL $ 218.00
H-321-100 100 mL $ 577.00

Description

Low Density Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >12 mg/ml with approximately 60 kDa protein. It works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-321
Storage/Handling store at 4°C. Do NOT freeze.
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H-321-25 25 mL $ 218.00
H-321-100 100 mL $ 577.00

Nickel Agarose Resin Cartridges (High Density)

Catalog ID Size Pricing
H-322-CA1 Cartridge: 1x5 mL $ 121.00
H-322-CA5 Cartridge: 5x5 mL $ 473.00

Description

Nickel Agarose Resin is available in a prepacked cartridge, specifically for use in ÄKTA or Profinia protein purification systems (see additional information for compatible systems).

It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-322
Storage/Handling store at 4°C. Do NOT freeze.
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H-322-CA1 Cartridge: 1x5 mL $ 121.00
H-322-CA5 Cartridge: 5x5 mL $ 473.00

Nickel HTC Agarose Beads

Catalog ID Size Pricing
R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Description

Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID R-202
Storage/Handling store at 4°C. Do NOT freeze.
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R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Nickel NTA Agarose Beads

There is no image for Nickel NTA Agarose Beads Nickel NTA Agarose Beads
Catalog ID Size Pricing
H-350-5 5 mL $ 59.00
H-350-10 10 mL $ 95.00
H-350-25 25 mL $ 207.00
H-350-50 50 mL $ 359.00
H-350-100 100 mL $ 678.00

Description

Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-350
Storage/Handling store at 4°C. Do NOT freeze.
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H-350-5 5 mL $ 59.00
H-350-10 10 mL $ 95.00
H-350-25 25 mL $ 207.00
H-350-50 50 mL $ 359.00
H-350-100 100 mL $ 678.00

Nickel NTA HTC Agarose Beads

There is no image for Nickel NTA HTC Agarose Beads Nickel NTA HTC Agarose Beads
Catalog ID Size Pricing
H-355-25 25 mL $ 278.00
H-355-100 100 mL $ 935.00
H-355-500 500 mL $ 4,023.00

Description

Nickel NTA HTC Agarose Resin has a binding capacity of ~60 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-355
Storage/Handling store at 4°C. Do NOT freeze.
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H-355-25 25 mL $ 278.00
H-355-100 100 mL $ 935.00
H-355-500 500 mL $ 4,023.00

Nickel NTA Magnetic Agarose Beads

There is no image for Nickel NTA Magnetic Agarose Beads Nickel NTA Magnetic Agarose Beads
Catalog ID Size Pricing
H-351-2 2 mL $ 97.00
H-351-5 5 mL $ 218.00
H-351-10 10 mL $ 396.00

Description

Nickel NTA Magnetic Agarose Beads are a rapid and easy small scale purification of histidine-tagged proteins. This resin consists of magnetic agarose derivatized with Nitrilotriacetic (NTA) and provides good properties working in native or denaturing conditions. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

NTA cross-linked resins consist of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent which provides a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-351
Storage/Handling store at 4°C. Do NOT freeze.
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H-351-2 2 mL $ 97.00
H-351-5 5 mL $ 218.00
H-351-10 10 mL $ 396.00

Zinc Agarose Beads (High Density)

Catalog ID Size Pricing
H-330-25 25 mL $ 218.00
H-330-100 100 mL $ 577.00

Description

High Density Zinc Agarose Resin is available as bulk resins for better customization. Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Zn2+. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-330
Storage/Handling store at 4°C. Do NOT freeze.
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H-330-25 25 mL $ 218.00
H-330-100 100 mL $ 577.00

Zinc Agarose Beads (Low Density)

Catalog ID Size Pricing
H-331-25 25 mL $ 218.00
H-331-100 100 mL $ 577.00

Description

Low Density Zinc Agarose Resin is available as bulk resins for better customization. Purification of proteins is a vital part of modern research. Impure extracts generally contain a wide range of proteins with diverse biological functions and different chemistry, which need to be separated.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Zn2+. The resulting resin (ready-to-use) is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-331
Storage/Handling store at 4°C. Do NOT freeze.
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H-331-25 25 mL $ 218.00
H-331-100 100 mL $ 577.00

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