Immunohistochemistry

BCIP Sodium salt is a water soluble form of BCIP. BCIP is a chromogenic substrate that is used to visualize the activity of alkaline phosphatase. When combined with NBT, BCIP produces a blue to violet insoluble end product and can be used in a variety of assays, including immunohistochemistry, Western blotting, Northern blotting and Southern blotting.

4-Nitrophenyl phosphate, disodium salt hexahydrate is a chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases and is used for determining alkaline phosphatase activity in human serum.

BCIP is a white to off-white powder that is used as a substrate for visualization of alkaline phosphatase. The end product is insoluble and purple when used with nitroblue tetrazolium. This final product can be visually detected. BCIP is DMF soluble.

DAB tetrahydrochloride hydrate is a water soluble form of 3-3'-diaminobenzidine and is used to stain for peroxidase activity. The intensity of DAB staining can be increased with the addition of cobalt, nickel or silver ions.

Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple-brown final color of the precipitates at the reaction sites.

NBT has also been used with X-Gal,or Salmon-Gal to speed up and enhance colony screening.

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.