Immunohistochemistry

Using immunohistochemistry, antibodies bind with antigens like proteins. It requires a staining agent like DAB, TMB or BCIP with an enzyme like alkaline phosphatase or horseradish peroxidase, which is more stable at room temperature. The enzyme catalyzes the reaction and the antigens become colored, which indicates the location of proteins. Using different antibodies allows you to choose which proteins to colorize, so a cell sample can be analyzed in multiple ways. Staining is especially useful in helping improve microscopy visibility. GoldBio provides staining agents and catalyzing enzymes at prices that help preserve your research budget.
Showing 1-6 of 6 items.

4-Nitrophenyl phosphate, disodium salt hexahydrate

Catalog ID Size Pricing
N-380-50 50 g $ 74.00
N-380-100 100 g $ 126.00
N-380-250 250 g $ 279.00
N-380-500 500 g $ 475.00

Description

4-Nitrophenyl phosphate, disodium salt hexahydrate is a chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases and is used for determining alkaline phosphatase activity in human serum.

Product Specifications

Catalog ID N-380
CAS # 333338-18-4
MW 371.15 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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N-380-50 50 g $ 74.00
N-380-100 100 g $ 126.00
N-380-250 250 g $ 279.00
N-380-500 500 g $ 475.00

BCIP

Catalog ID Size Pricing
B-500-1 1 g $ 63.00
B-500-10 10 g $ 272.00
B-500-25 25 g $ 675.00
B-500-50 50 g $ 1,281.00

Description

BCIP is a white to off-white powder that is used as a substrate for visualization of alkaline phosphatase. The end product is insoluble and purple when used with nitroblue tetrazolium. This final product can be visually detected. BCIP is DMF soluble.

Product Specifications

Catalog ID B-500
CAS # 6578-06-9
MW 433.63 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
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B-500-1 1 g $ 63.00
B-500-10 10 g $ 272.00
B-500-25 25 g $ 675.00
B-500-50 50 g $ 1,281.00

DAB Tetrahydrochloride Hydrate

Catalog ID Size Pricing
D-320-1 1 g $ 44.00
D-320-5 5 g $ 72.00
D-320-10 10 g $ 137.00
D-320-25 25 g $ 317.00

Description

DAB tetrahydrochloride hydrate is a water soluble form of 3-3'-diaminobenzidine and is used to stain for peroxidase activity. The intensity of DAB staining can be increased with the addition of cobalt, nickel or silver ions.

Product Specifications

Catalog ID D-320
CAS # 868272-85-9
MW 360.11 g/mol
Grade REAGENT GRADE
Storage/Handling Store at -20°C.
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D-320-1 1 g $ 44.00
D-320-5 5 g $ 72.00
D-320-10 10 g $ 137.00
D-320-25 25 g $ 317.00

Nitrotetrazolium blue chloride (NBT)

Catalog ID Size Pricing
NBT1 1 g $ 48.00
NBT2.5 2.5 g $ 98.00
NBT5 5 g $ 158.00
NBT10 10 g $ 254.00

Description

Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple-brown final color of the precipitates at the reaction sites.

NBT has also been used with X-Gal,or Salmon-Gal to speed up and enhance colony screening.

Product Specifications

Catalog ID NBT
CAS # 298-83-9
MW 817.65 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C. Protect from light.
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NBT1 1 g $ 48.00
NBT2.5 2.5 g $ 98.00
NBT5 5 g $ 158.00
NBT10 10 g $ 254.00

TMB dihydrochloride

Catalog ID Size Pricing
TMBHCL2 2 g $ 68.00
TMBHCL10 10 g $ 299.00
TMBHCL25 25 g $ 576.00
TMBHCL50 50 g $ 885.00

Description

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.

Product Specifications

Catalog ID TMBHCL
CAS # 207738-08-7
MW 313.27 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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TMBHCL2 2 g $ 68.00
TMBHCL10 10 g $ 299.00
TMBHCL25 25 g $ 576.00
TMBHCL50 50 g $ 885.00

TMB, free base

Catalog ID Size Pricing
TMBF10 10 g $ 65.00
TMBF25 25 g $ 153.00
TMBF50 50 g $ 240.00
TMBF100 100 g $ 436.00

Description

TMB is an excellent colorimetric substrate for detection of horseradish peroxidase labeled probes and is used with peroxidase and peroxidase-coupled systems, particularly in ELISA techniques. It produces a soluble end product, which is blue in color and can be read spectrophotometrically. TMB has also been used in the quantitative determination of hemoglobin and in cytochemical staining for peroxidase. TMB is a hepatitis B antigen.

Product Specifications

Catalog ID TMBF
CAS # 54827-17-7
MW 240.35 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at 4°C. Protect from light.
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TMBF10 10 g $ 65.00
TMBF25 25 g $ 153.00
TMBF50 50 g $ 240.00
TMBF100 100 g $ 436.00

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