Loading Dye

Gold Biotechnology has affordable chemical dyes for use in loading dyes in gel electrophoresis. GoldBio is committed to providing superior products with superior pricing. We offer Bromophenol Blue, Orange G and Xylene Cyanol FF which migrate similar to DNA bands of 500bp, 50bp and 4kb respectively in a 1% agarose gel. These dyes may be mixed to ficoll or glycerol to create a precise, customized loading dye specific to your research needs.
Showing 1-7 of 7 items.

6X Blue Loading Dye

Catalog ID Size Pricing
L002-5 5 mL $ 26.00
L002-10 2 x 5 mL $ 47.00
L002-20 4 x 5 mL $ 90.00
L002-50 10 x 5 mL $ 212.00

Description

GoldBio's 6× Blue DNA Loading Dye is pre-mixed buffer designed for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 4,000 bp and 500 bp on a standard 1% TAE agarose gel respectively (800 bp and 150 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Product Specifications

Catalog ID L002
Storage/Handling Store at 4°C.
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L002-5 5 mL $ 26.00
L002-10 2 x 5 mL $ 47.00
L002-20 4 x 5 mL $ 90.00
L002-50 10 x 5 mL $ 212.00

6X GelRed™ Prestain Loading Buffer with Blue Tracking Dyes

Catalog ID Size Pricing
G-730-1 1 mL $ 78.00

Description

GelRed™ is an ultra-sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed™ Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.

6X GelRed™ Prestain Loading Buffer with Blue Tracking Dyes contains two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel. For smaller tracking dyes, see Catalog # G-735 (6X GelRed™ Prestain Loading Buffer with Orange Tracking Dyes).

GelRed™ and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed™ is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed™ is compatible with downstream applications such as sequencing and cloning. GelRed™ is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.

Product Specifications

Catalog ID G-730
Storage/Handling Store at 4°C. Protect from light.
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G-730-1 1 mL $ 78.00

6X GelRed™ Prestain Loading Buffer with Orange Tracking Dye

Catalog ID Size Pricing
G-735-1 1 mL $ 78.00

Description

GelRed™ is an ultra-sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EtBr). 6X GelRed™ Prestain Loading Buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. The 6X prestain loading buffer is added to samples in place of gel loading buffer and eliminates the need to add fluorescent DNA dye to the agarose gel during casting.

6X GelRed™ Prestain Loading Buffer with Orange Tracking Dye contains an orange electrophoresis tracking dye that run at approximately 50 bp in a 1% agarose gel. For larger tracking dyes, see Catalog # G-730 (6X GelRed™ Prestain Loading Buffer with Blue Tracking Dyes).

GelRed™ and EtBr have virtually the same spectra, so you can directly replace EtBr with GelRed without changing your existing imaging system. In addition, GelRed™ is far more sensitive than EtBr, which cannot be used in DNA loading buffer to prestain DNA. GelRed™ is compatible with downstream applications such as sequencing and cloning. GelRed™ is efficiently removed from DNA by commercial gel extraction kits or by phenol/chloroform extraction and ethanol precipitation.

Product Specifications

Catalog ID G-735
Storage/Handling Store at 4°C. Protect from light.
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G-735-1 1 mL $ 78.00

6X Green Loading Dye

Catalog ID Size Pricing
L001-5 5 ml $ 26.00
L001-10 2 x 5 ml $ 47.00
L001-20 4 x 5 ml $ 90.00
L001-50 10 x 5 ml $ 212.00

Description

GoldBio's 6× Green DNA Loading Dye is a pre-mixed buffer for tracking DNA samples during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Orange G) for tracking the DNA migration. The Xylene cyanol FF and Orange G migrate at approximately 4,000 bp and 50 bp on a standard 1% TAE agarose gel respectively (800 bp and 30 bp on a 2% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease.

Product Specifications

Catalog ID L001
Storage/Handling Store desiccated at -20°C.
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L001-5 5 ml $ 26.00
L001-10 2 x 5 ml $ 47.00
L001-20 4 x 5 ml $ 90.00
L001-50 10 x 5 ml $ 212.00

Bromophenol Blue Free Acid, ACS Grade

Catalog ID Size Pricing
B-092-5 5 g $ 28.00
B-092-25 25 g $ 35.00
B-092-100 100 g $ 86.00

Description

Bromophenol blue is a dye for tracking proteins and nucleic acids in gel electrophoresis. Bromophenol blue migrates at about the same rate as ~500bp DNA in a 1% agarose gel and at the buffer front in protein polyacrylamide gels.

Product Specifications

Catalog ID B-092
CAS # 115-39-9
MW 669.96 g/mol
Storage/Handling Store desiccated at room temperature.
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B-092-5 5 g $ 28.00
B-092-25 25 g $ 35.00
B-092-100 100 g $ 86.00

Orange G, Sodium Salt

Catalog ID Size Pricing
O-020-100 100 g $ 48.00

Description

Orange G, sodium salt is a reagent used as a tracking dye for nucleic acids in gel electrophoresis. Orange G migrates at about the same rate as a 50 bp DNA fragment on a 1% agarose gel.

Product Specifications

Catalog ID O-020
CAS # 1936-15-8
MW 452.37 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at room temperature.
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O-020-100 100 g $ 48.00

Xylene Cyanol FF, Ultra Pure

Catalog ID Size Pricing
X-300-5 5 g $ 30.00

Description

Xylene cyanol FF is commonly used as a tracking dye in DNA gels and runs at approximately the same rate as a 4 kb DNA fragment on a 1% agarose gel.

Product Specifications

Catalog ID X-300
CAS # 2650-17-1
MW 538.61 g/mol
Storage/Handling Store desiccated at room temperature.
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X-300-5 5 g $ 30.00

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