Luciferase Assay Kits

GoldBio's premier selection of high-quality luciferase assay kits are a great addition for your biolumenescent research! Our catalog includes dual assay kits, flash type kits and glowing options. Our luciferase assay kits use GoldBio’s highly published luciferin and provide researchers with convenience and sensitivity.

Showing 1-7 of 7 items.

5X Luciferase Lysis Buffer

There is no image for 5X Luciferase Lysis Buffer 5X Luciferase Lysis Buffer
Catalog ID Size Pricing
L-740-15 15 mL $ 21.00

Description

5X Luciferase Lysis Buffer is a concentrated cell lysis buffer that is designed for use with GoldBio’s Illumination™ Firefly Luciferase Enhanced Assay Kit.The kit is supplied with enough lysis buffer to perform the stated number of assays.This 5X Luciferase Lysis Buffer is sold separately for use with the kits when more lysis buffer is required.

5X Luciferase Lysis Buffer is not compatible with Renilla Luciferase or the Illumination™ Renilla Luciferase Enhanced Assay Kit.For Renilla luciferase assays, use 5X Passive Lysis Buffer (GoldBio Catalog # L-745).

Storage/Handing: Store at -20°C.

Product may be shipped at ambient temperature without reducing performance. Please place at the recommended storage conditions upon receipt of the product.

Product Specifications

Catalog ID L-740
Storage/Handling Store at -20°C.
View
Quick Add
L-740-15 15 mL $ 21.00

5X Passive Lysis Buffer

There is no image for 5X Passive Lysis Buffer 5X Passive Lysis Buffer
Catalog ID Size Pricing
L-745-30 30 mL $ 27.00

Description

5X Passive Lysis Buffer is a concentrated cell lysis buffer that is designed for use with GoldBio’s Illumination™ Renilla Luciferase Enhanced Assay Kit.The kit is supplied with enough lysis buffer to perform the stated number of assays.This 5X Passive Lysis Buffer is sold separately for use with the kits when more lysis buffer is required.

5X Passive Lysis Buffer is not compatible with Firefly Luciferase or the Illumination™ Luciferase Enhanced Assay Kit.For Firefly luciferase assays, use 5X Luciferase Lysis Buffer (GoldBio Catalog # L-740).

Product Specifications

Catalog ID L-745
Storage/Handling Store at -20°C.
View
Quick Add
L-745-30 30 mL $ 27.00

Illumination™ Renilla Luciferase Enhanced Assay Kit

Catalog ID Size Pricing
I-925-50 50 Assays $ 63.00
I-925-150 150 Assays $ 118.00
I-925-1000 1000 Assays $ 529.00

Description

GoldBio’s Illumination™ Renilla Luciferase Enhanced Assay Kit is specifically designed for the measurement of Renilla luciferase in transfected cell reporter gene assays. This kit is enhanced with a water soluble coelenterazine that is easier to use and store than other conventional coelenterazine products.

Renilla luciferase is a commonly used bioluminescent reporter gene that is used to study gene regulation and function both in vivo as well as in vitro. Renilla luciferase is a monomeric 36 kDa protein isolated from the sea pansy, Renilla reniformis, which catalyzes coelenterazine with oxygen to produce a blue light (480 nm). There is no need for any post-translational modifications and the protein may be used as a gene reporter immediately after translation. Our Enhanced Assay Kit includes a special buffer which is designed to yield reliable, linear measurements of Renilla luciferase activity with maximum sensitivity and minimal autoluminescence. This kit is a flash-type luminescent assay, and requires measurement immediately after adding the substrate to the sample.

One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Passive Lysis Buffer (GoldBio Catalog # L-745) can be purchased separately.

Product Specifications

Catalog ID I-925
Storage/Handling Store the kit at -80°C.
View
Quick Add
I-925-50 50 Assays $ 63.00
I-925-150 150 Assays $ 118.00
I-925-1000 1000 Assays $ 529.00

Illumination™ Dura-Luc Lyophilized Firefly HTS Assay Kit

There is no image for Illumination™ Dura-Luc Lyophilized Firefly HTS Assay Kit Illumination™ Dura-Luc Lyophilized Firefly HTS Assay Kit
Catalog ID Size Pricing
I-946-120 120 Assays $ 86.00
I-946-1000 1000 Assays $ 545.00
I-946-10000 10,000 Assays $ 3,011.00

Description

GoldBio’s Illumination™ Dura-Luc Lyophilized Firefly HTS Assay Kit contains a proprietary formulation that modifies and stabilizes the enzymatic reaction to produce a long lasting bioluminescent signal by preventing the formation of adenyl-oxyluciferin at the enzyme surface. The Dura-Luc system is a highly sensitive and homogenous luciferase reporter assay that has a signal half-life of three hours for the best quantification of expression in mammalian cell lines, allowing for batch processing and plate to plate comparisons in high-throughput screening experiments.

Firefly Luciferase Assays are one of the leading reporter assays in the world in the measurement of gene function and gene regulation as well as being widely used in pharmaceutical screening. The luciferase assays are sensitive and convenient due to the absence of endogenous luciferase activity in most cell types and tissue. Firefly luciferase is a monomeric 62 kDa protein typically isolated from the firefly, Photinus pyralis, which catalyzes the ATP-dependent D-luciferin in the presence of oxygen and Mg2+ to oxyluciferin producing a yellow to greenish light (~560 nm).

The Dura-Luc Lyophilized Firefly Kit contains an assay buffer that is provided in a lyophilized form for economical shipping and more convenient storage. A reconstitution buffer is also provided with this kit.

Product Specifications

Catalog ID I-946
Storage/Handling Store the kit at -20°C.
View
Quick Add
I-946-120 120 Assays $ 86.00
I-946-1000 1000 Assays $ 545.00
I-946-10000 10,000 Assays $ 3,011.00

Illumination™ Firefly & Renilla Luciferase Enhanced Assay Kit

Catalog ID Size Pricing
I-920-50 50 Assays $ 103.00
I-920-150 150 Assays $ 188.00
I-920-1000 1000 Assays $ 908.00

Description

GoldBio’s Illumination™ Firefly & Renilla Luciferase Enhanced Assay Kit is a combined luciferase assay allowing the sequential measurement of Firefly and Renilla luciferase activity in the same sample while still retaining the highest degree of sensitivity, reliability and linearity that you want in your assay. Firefly activity is measured first, then the Renilla Luciferase Assay Buffer is added to the sample to simultaneously extinguish all firefly luciferase activity to the level of untransformed cells and begins the Renilla luciferase activity. This great luciferase combo allows the sequential measurement of both luciferase assays in the same sample without additional hassle.

This kit is a flash-type luminescent assay, and requires measurement immediately after adding the detection reagents to the sample. The Enhanced Assay Kit includes our water soluble coelenterazine that is easier to use and store than other conventional coelenterazine products.

One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Passive Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well.

Kit components

  • 1X Passive Lysis Buffer
  • Firefly Luciferase Assay Buffer
  • Renilla Luciferase Assay Buffer
  • GoldBio D-Luciferin
  • GoldBio Enhanced Coelenterazine

Product Specifications

Catalog ID I-920
Storage/Handling Store the kit at -80°C.
View
Quick Add
I-920-50 50 Assays $ 103.00
I-920-150 150 Assays $ 188.00
I-920-1000 1000 Assays $ 908.00

Illumination™ Firefly Luciferase Enhanced Assay Kit

Catalog ID Size Pricing
I-930-50 50 Assays $ 58.00
I-930-150 150 Assays $ 73.00
I-930-1000 1000 Assays $ 397.00

Description

GoldBio’s Illumination™ Firefly Luciferase Enhanced Assay Kit is specifically designed for the simple and efficient quantification of firefly luciferase reporter enzyme activity from cultured cells with the highest degree of sensitivity, reliability and linearity. This kit is a flash-type luminescent assay, and requires measurement immediately after adding the substrate to the sample.Our Enhanced Assay Kit includes our improved 5X Luciferase Lysis Buffer which provides a better working solution stability compared to other firefly assay kits.

One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Luciferase Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Luciferase Lysis Buffer (GoldBio Catalog # L-740) can be purchased separately.

Kit components

  • 5X Luciferase Lysis Buffer
  • Firefly Luciferase Assay Buffer
  • GoldBio D-Luciferin

Product Specifications

Catalog ID I-930
Storage/Handling Store the kit at -80°C.
View
Quick Add
I-930-50 50 Assays $ 58.00
I-930-150 150 Assays $ 73.00
I-930-1000 1000 Assays $ 397.00

Illumination™ Lyophilized Firefly Luciferase Enhanced Assay Kit

Catalog ID Size Pricing
I-935-150 150 Assays $ 73.00
I-935-1000 1000 Assays $ 397.00

Description

GoldBio’s Illumination™ Lyophilized Firefly Luciferase Enhanced Assay Kit is specifically designed for the simple and efficient quantification of firefly luciferase reporter enzyme activity from cultured cells with the highest degree of sensitivity, reliability and linearity. In addition, the Firefly Luciferase Assay Buffer is lyophilized for more convenient shipping and storage.

This kit is a flash-type luminescent assay, and requires measurement immediately after adding the substrate to the sample.Our Enhanced Assay Kit includes our improved 5X Luciferase Lysis Buffer which provides a better working solution stability compared to other firefly assay kits.

One Assay can be performed in a single tube or an individual well on a 96 well plate. The kits include enough Luciferase Lysis Buffer to perform the stated number of assays in plate sizes ranging from 96-well to 24-well. If needed, additional 5X Luciferase Lysis Buffer (GoldBio Catalog # L-740) can be purchased separately.

Kit components

  • 5X Luciferase Lysis Buffer
  • Firefly Luciferase Assay Buffer (lyophilized)
  • GoldBio D-Luciferin

Product Specifications

Catalog ID I-935
Storage/Handling Store the kit at -20°C.
View
Quick Add
I-935-150 150 Assays $ 73.00
I-935-1000 1000 Assays $ 397.00

Join our list to receive promos and articles.

Join Now