Lysis Buffers

Gold Biotechnology provides a well-rounded selection of lysis buffer solutions for all of your protein recovery requirements. Our buffers are designed exclusively for the lysis of bacterial, animal, plants or yeast cultures. Your purified proteins can then be used for enzyme assays, chromotographical analysis, electrophoreses, and many other downstream applications. GoldBio lysis buffers are based on proprietary combinations of buffering agents, mild detergents and various salts which enhance the extraction of proteins and also maintain the stability of biological activities of the lysed proteins.
Showing 1-5 of 5 items.

Bacterial Cell Lysis Buffer

Catalog ID Size Pricing
GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-176-100 100 Preps $ 165.00
GB-176-250 250 Preps $ 289.00
GB-176-500 500 Preps $ 533.00

Description

The Bacterial Cell Lysis Kit has been developed for the extraction of biologically active, soluble proteins and inclusion bodies from bacterial cells. The Bacterial Lysis Buffer is a proprietary improvement on the lysozyme based lysis method, which allows extraction of soluble proteins and concurrent removal of nucleic acids (DNA & RNA) released during cell lysis. The kit’s lysis eliminates viscosity build-up, allowing effective clarification with lower centrifugal forces.

Product Specifications

Catalog ID GB-177
Storage/Handling Store at 4°C. Do NOT freeze.
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GB-177-100 100 mL $ 58.00
GB-177-500 500 mL $ 113.00
GB-176-100 100 Preps $ 165.00
GB-176-250 250 Preps $ 289.00
GB-176-500 500 Preps $ 533.00

Mammalian Cell Lysis Buffer

Catalog ID Size Pricing
GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Description

Product Specifications

Catalog ID GB-180
Storage/Handling Store at 4°C.
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GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Tissue Cell Lysis Buffer

Catalog ID Size Pricing
GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Description

Tissue Cell Lysis Buffer has been developed for extraction of total soluble protein from fresh or frozen animal tissues. Tissue Cell Lysis Buffer is based on an organic buffer, which utilizes a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on the application, additional agents such as chelating agents, reducing agents and protease inhibitors may be added into Tissue Cell Lysis Buffer. Tissue Cell Lysis Buffer reagent has been tested for use with a wide variety of animal tissues.

Product Specifications

Catalog ID GB-181
Storage/Handling Store at 4°C.
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GB-181-100 100 mL $ 58.00
GB-181-500 500 mL $ 113.00

Yeast Lysis Buffer Kit plus Zymolyase

Catalog ID Size Pricing
GB-178 100 Preps $ 171.00

Description

No description available

Product Specifications

Catalog ID GB-178
Storage/Handling Store at -20°C.
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GB-178 100 Preps $ 171.00

Yeast Spheroplast Lysis Buffer

Catalog ID Size Pricing
GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

Description

The Yeast Spheroplast Lysis Buffer is useful for extraction of soluble proteins from spheroplast cells. It is a proprietary improvement on the Zymolyase® based spheroplast preparation and extraction of soluble proteins from yeast cells.

Yeast Lysis Buffer is based on organic buffering agents that utilize a mild non-ionic detergent and a proprietary combination of various salts and agents to enhance extraction and stability of proteins. Depending on application, additional agents such as reducing agents, chelating agents, and protease inhibitors may be added into Yeast Lysis Buffer. The Yeast Lysis Buffer has been optimized with Saccharomyces cerevisiae and has been tested on several widely used yeast strains. It also works well with both gram-positive and gram-negative bacteria.

The proprietary combination of this reagent provides a simple and versatile method of yeast protein extraction. Yeast Lysis Buffer eliminates the need for laborious glass bead lysis of yeast cells.

Product Specifications

Catalog ID GB-179
Storage/Handling Store at -20°C.
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GB-179-100 100 mL $ 58.00
GB-179-500 500 mL $ 113.00

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