Nickel

Nickel agarose beads are used in His-tag purifications. GoldBio’s IDA cross-linked agarose resins consist of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. Nickel divalent metal ions have a good affinity with medium specificity binding capacity and is the metal ion most commonly used in His-tag protein purification. GoldBio offers nickel agarose beads in both a low and high density form to allow better specificity or higher affinity, respectively.
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Highest Density Nickel

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Catalog ID Size Pricing
H-390-25 25 mL $ 333.00
H-390-100 100 mL $ 1,280.00
H-390-500 500 mL $ 5,748.00

Description

GoldBio’s new Highest Density Nickel Agarose is a high capacity resin manufactured with a polychelator ligand. The product was developed to work in the presence of EDTA and DTT without any loss of performance. Its specificity and stability allows a one-step purification, eliminating the need for pretreatment of samples that cause nickel stripping.

Our Highest Density Nickel Agarose is supplied as a suspension in 50% aqueous suspension containing 20% ethanol. Purifications can be performed using 20mM EDTA and 20mM DTT and can work in both native as well as denaturing conditions.

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H-390-25 25 mL $ 333.00
H-390-100 100 mL $ 1,280.00
H-390-500 500 mL $ 5,748.00

Nickel Agarose Beads (High Density)

Catalog ID Size Pricing
H-320-5 5 mL $ 53.00
H-320-10 10 mL $ 92.00
H-320-25 25 mL $ 205.00
H-320-50 50 mL $ 341.00
H-320-100 100 mL $ 609.00
H-320-500 500 mL $ 2,494.00

Description

Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-320
Storage/Handling store at 4°C. Do NOT freeze.
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H-320-5 5 mL $ 53.00
H-320-10 10 mL $ 92.00
H-320-25 25 mL $ 205.00
H-320-50 50 mL $ 341.00
H-320-100 100 mL $ 609.00
H-320-500 500 mL $ 2,494.00

Nickel Agarose Beads (Low Density)

Catalog ID Size Pricing
H-321-25 25 mL $ 234.00
H-321-100 100 mL $ 619.00

Description

Low Density Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >12 mg/ml with approximately 60 kDa protein. It works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-321
Storage/Handling store at 4°C. Do NOT freeze.
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H-321-25 25 mL $ 234.00
H-321-100 100 mL $ 619.00

Nickel HTC Agarose Beads

Catalog ID Size Pricing
R-202-25 25 mL $ 291.00
R-202-100 100 mL $ 735.00
R-202-500 500 mL $ 2,796.00

Description

Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID R-202
Storage/Handling store at 4°C. Do NOT freeze.
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R-202-25 25 mL $ 291.00
R-202-100 100 mL $ 735.00
R-202-500 500 mL $ 2,796.00

Nickel NTA Agarose Beads

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Catalog ID Size Pricing
H-350-5 5 mL $ 68.00
H-350-10 10 mL $ 110.00
H-350-25 25 mL $ 238.00
H-350-50 50 mL $ 411.00
H-350-100 100 mL $ 774.00

Description

Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-350
Storage/Handling store at 4°C. Do NOT freeze.
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H-350-5 5 mL $ 68.00
H-350-10 10 mL $ 110.00
H-350-25 25 mL $ 238.00
H-350-50 50 mL $ 411.00
H-350-100 100 mL $ 774.00

Nickel NTA HTC Agarose Beads

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Catalog ID Size Pricing
H-355-25 25 mL $ 309.00
H-355-100 100 mL $ 1,032.00
H-355-500 500 mL $ 4,439.00

Description

Nickel NTA HTC Agarose Resin has a binding capacity of ~60 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-355
Storage/Handling store at 4°C. Do NOT freeze.
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H-355-25 25 mL $ 309.00
H-355-100 100 mL $ 1,032.00
H-355-500 500 mL $ 4,439.00

Nickel NTA Magnetic Agarose Beads

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Catalog ID Size Pricing
H-351-2 2 mL $ 108.00
H-351-5 5 mL $ 242.00
H-351-10 10 mL $ 438.00

Description

Nickel NTA Magnetic Agarose Beads are a rapid and easy small scale purification of histidine-tagged proteins. This resin consists of magnetic agarose derivatized with Nitrilotriacetic (NTA) and provides good properties working in native or denaturing conditions. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

NTA cross-linked resins consist of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent which provides a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-351
Storage/Handling store at 4°C. Do NOT freeze.
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H-351-2 2 mL $ 108.00
H-351-5 5 mL $ 242.00
H-351-10 10 mL $ 438.00

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