PCR Products

GoldBio’s 2’-Deoxynucleoside 5’-triphosphate mix is an aqueous solution containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM. This mixture has been tested for performance in a wide size-range of PCR templates with Taq and Pfu DNA polymerases.

The dNTP solution is ideal for PCR, cDNA synthesis, DNA sequencing and labeling procedures, and it has been performance tested with Q-PCR and long PCR (30 kb).

EvaGreen® dye is a green fluorescent nucleic acid dye with features that make the dye useful for several applications including qPCR and DNA melt curve analysis, real-time monitoring of thermophilic helicase-dependent amplification (tHDA), and capillary gel electrophoresis. The DNA-bound dye has excitation and emission spectra very close to those of fluorescein (FAM) or SYBR® Green I, making the dye readily compatible with instruments equipped with the 488 nm argon laser or any visible light excitation with wavelength in the region. EvaGreen® dye is extremely stable both thermally and hydrolytically, providing convenience during routine handling. The dye is essentially nonfluorescent by itself, but becomes highly fluorescent upon binding to dsDNA. EvaGreen® dye is nonmutagenic and noncytotoxic by being completely impermeable to cell membranes, making it a great alternative to SYBR® Green I, which enters cell rapidly and is known to be a powerful mutation-enhancer (Ohta, et al. (2001). Mutation Research, 492(1), 91-97).

The unique properties of EvaGreen® dye have made it particularly useful in quantitative real-time PCR (qPCR) applications. Compared with the widely used SYBR® Green I, EvaGreen® dye is generally less inhibitory toward PCR and less likely to cause nonspecific amplification. As a result, EvaGreen® dye can be used at a much higher dye concentration than SYBR® Green I, resulting in a high resolution signal for droplet digital PCR, real-time PCR and melt curve analysis.

GoldBio's First Strand cDNA synthesis kit includes our 5x Master Mix which contains: Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl₂ and protein stabilizers. Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nuclease-free water. An anchored Oligo-dT primer forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-55ºC.

Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue (see Figure 2).

Goof-Proof™ allows you to see quickly and easily whether you forgot to add the master mix or the template to any of your reactions, preventing pipetting mistakes and wasted time, reagents and money. The 2X Goof-Proof™ Master Mix can also be used without the Template Buffer, if desired.

Goof-Proof™ qPCR Master Mix is an EvaGreen dye-based hot start master mix for use in DNA melt curve analysis and in Real Time PCR (RT-PCR) applications. Goof-Proof™ is a mistake-prevention master mix which contains a low concentration of blue dye. Additionally, Goof-Proof™ includes a DNA template buffer which has a higher concentration of blue dye. With the addition of the 2X Goof-Proof™ Master Mix to the reaction, it will appear light blue (see Figure 1). And when you add the template, containing DNA template buffer, the color will turn darker blue.

Goof-Proof™ Universal Probe Master Mix is a high-performance 2X master mix for fluorescent probe-based PCR applications such as SNP genotyping and quantitation. Goof-Proof™ has broad instrument compatibility, can be used in both standard and fast protocols and shows a high degree of consistency in both singleplex and multiplex reactions. The Goof-Proof™ Universal Probe Master Mix is perfectly designed for all fluorescent probe technologies, including displacement probes such as Molecular Beacon and hydrolysis probes such as TaqMan®.

Goof-Proof™ Universal Probe Master Mix with ROX is a high-performance 2X master mix for fluorescent probe-based PCR applications such as SNP genotyping and quantitation. Goof-Proof™ has broad instrument compatibility, can be used in both standard and fast protocols and shows a high degree of consistency in both singleplex and multiplex reactions. The Goof-Proof™ Universal Probe Master Mix is perfectly designed for all fluorescent probe technologies, including displacement probes such as Molecular Beacon and hydrolysis probes like TaqMan®.

Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer that has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

Product Includes

• Hot Start Taq 2x master mix
• 5x GC Enhancer

Taq Purity: ≥98% (assessed by SDS-PAGE with Coomassie blue staining)

Taq Concentration (1x): 0.1 units/μl
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP into acid-insoluble form in 30 minutes at 72ºC.

i7® Hot Start High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. i7 Hot Start High-Fidelity DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8 × 10²-fold lower than Taq DNA polymerase, and significantly lower than the error rates of other proofreading enzymes in the marketplace. i7® Hot Start High-Fidelity DNA Polymerase is ideal for cloning and can be used for long (up to 20 kb) or difficult amplicons. This product is supplied with a proprietary 2.5X PCR reaction buffer containing MgCl₂ with a final (1X) concentration of 2mM. This buffer allows for amplification of non GC rich templates as well as GC rich templates up to 84%.

Applications

• Long and difficult template DNA amplification
• Cloning
• High-fidelity PCR

i7™ High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8×10² fold lower than Taq DNA polymerase. i7™ high-fidelity DNA polymerase is ideal for cloning and can be used for long (up to 20 kb) or difficult amplicons. This product is supplied with a proprietary 5x i7 PCR reaction buffer containing MgCl₂ (final (1x) concentration of 2mM), and 5x GC enhancer that enables efficient amplification of GC rich templates up to 84%.

InstantAmp™ Plant Direct PCR Kit is designed to be the simplest and best method to amplify DNA fragments from plant tissues. The advanced InstantAmp™ formulation provides higher specificity and higher sensitivity without needing complicated DNA purification steps as well as providing faster PCR cycling conditions without compromising the yield in your PCR reaction. InstantAmp™ Plant Direct PCR Kit is the best and most powerful tool for any plant-based high-throughput genotyping, DNA amplification, and genome analysis.

GoldBio's One Step RT-PCR kit combines two powerful mixtures: i) Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.

One step RT-qPCR kit combines two powerful mixtures: i) Reverse Transcriptase and ii) SYBR^® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.

The One Step RT-qPCR kit is used for detection of SARS-CoV-2 in vitro. The approach used for detection is by Real-Time quantitative PCR (RT-qPCR). The SARS-CoV-2 detection kit allows cDNA synthesis and qPCR in a single tube. The kit provides an improved RT-qPCR efficiency, wider dynamic range, superior sensitivity and specificity. In addition, the kit contains CDC recommended primers/probe sets for SARS-CoV-2 and can be used to detect SARS-CoV-2 in respiratory specimens; such as sputum, nasopharyngeal, orophayringel aspirates, washes or swabs and tracheal aspirates.

Our PCR Cloning kit utilizes cloning technology that is simple, rapid and highly efficient which allows direct cloning of any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by our high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step within short times (usually 10-30 minutes) with more than 95% cloning efficiency.

Applications

• Clone any insert at any site within any vector
• Restriction enzyme and phosphatase free system
• Joining multiple large fragments at once
• Precise insertion at a desired orientation
• Rapid and high efficiency with >95% positive clones

Pfu DNA polymerase is a heat stable DNA polymerase which has 5´→ 3´ DNA polymerase and 3´→ 5´ exonuclease (proofreading) activities. Pfu DNA polymerase retains the high fidelity, sensitivity and processivity with an error rate six-fold lower than Taq DNA polymerase, and significantly lower than the error rates of most other proofreading enzymes or DNA polymerase mixtures. This product is supplied with an optional 5X GC Enhancer that increases the amplification of GC rich templates up to 84%.

GoldBio's qPCR 2X Master Mix with SYBR Green^® is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The master mix contains integrated chemically-modified hot start Taq DNA polymerase, SYBR^® Green I fluorescent dye, ROX dye, MgCl₂, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

GoldBio's Reverse Transcriptase (RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. Our RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.

T5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA. The mode of action of T5 Exonuclease in vivo may be analogous to that of the 5´→3´ exonuclease activity of E. coli DNA Polymerase I.