Protease Inhibitor Cocktails

GoldBio’s protease inhibitor cocktails are ready-to-use solutions that contain irreversible and reversible protease inhibitors at optimized concentrations to inhibit serine and cysteine proteases, as well as other proteases. These cocktails are available EDTA free, making it perfect for 2D gel analysis, or they are available with an optional EDTA solution for the inhibition of metalloproteases.
Showing 1-12 of 12 items.

ProBlock™ Gold 2D Protease Inhibitor Cocktail (EDTA Free) [100X]

Catalog ID Size Pricing
GB-109-1 1 mL $ 173.00

Description

ProBlock™ Gold 2D is a protease inhibitor cocktail specifically developed for sample preparation for IEF/2D- studies. No EDTA is used, which allows optimal action of nuclease activity for removing nucleic acids from the samples.

ProBlock™ Gold 2D contains both irreversible and reversible protease inhibitors and inhibits serine, cysteine and metalloproteases etc. Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold 2D shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins extracted from animal cells / tissues, plants, yeast and bacteria etc.

ProBlock™ Gold 2D at 1X concentration in extraction buffer at pH 7-8 inhibits over 90% of protease activities.

Product Specifications

Catalog ID GB-109
Storage/Handling Store at -20°C.
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GB-109-1 1 mL $ 173.00

ProBlock™ Gold Bacterial 2D Protease Inhibitor Cocktail [100X]

Catalog ID Size Pricing
GB-376-1 1 mL $ 68.00
GB-376-5 5 mL $ 247.00

Description

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail. ProBlock™ Gold Bacterial 2D contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during recombinant protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit metallo, serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of Bacterial ProteaseArrest™ inhibits over 90% of protease activities.

ProBlock™ Gold Bacterial 2D cocktail does not use EDTA as its metalloprotease inhibitor as it would inhibit the activity of proteins that require divalent cations (Ca2+, Mg2+, or Mn2+) for their biological activity. In addition, EDTA would inhibit the purification of proteins using immobilized metal affinity chromatography (IMAC), for example His tagged or CBP tagged proteins. ProBlock™ Gold Bacterial 2Dcocktail is compatible with immobilized metal affinity chromatography.

Product Specifications

Catalog ID GB-376
Storage/Handling Store at 4°C.
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GB-376-1 1 mL $ 68.00
GB-376-5 5 mL $ 247.00

ProBlock™ Gold Bacterial Protease Inhibitor Cocktail [100X]

Catalog ID Size Pricing
GB-330-1 1 mL $ 70.00
GB-330-5 5 mL $ 252.00

Description

ProBlock™ Gold Bacterial Protease Inhibitor Cocktail contains optimized concentrations of various bacterial protease inhibitors, which provide excellent inhibition of protease activities during protein purification from bacteria. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine and cysteine proteases. It also contains specific inhibitors for bacterial proteases, such as aspartic proteases and aminopeptidases. A 1X concentration of ProBlock™ Gold Bacterial inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Bacterial is supplied additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

Product Specifications

Catalog ID GB-330
Storage/Handling Store at 4°C.
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GB-330-1 1 mL $ 70.00
GB-330-5 5 mL $ 252.00

ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail

GB-116 - Protease Inhibition
Catalog ID Size Pricing
GB-116-2 2 mL $ 139.00
GB-116-5 5 mL $ 285.00
GB-116-10 10 mL $ 499.00

Description

GoldBio’s new ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail is specifically formulated for the most demanding protein purification applications. The optimized concentrations of the various inhibitors in our Extra Strength cocktail deliver an enhanced dose of protease inhibition for the toughest research experiments. ProBlock™ Gold Extra Strength contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases.

Product Specifications

Catalog ID GB-116
Storage/Handling Store at 4°C.
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GB-116-2 2 mL $ 139.00
GB-116-5 5 mL $ 285.00
GB-116-10 10 mL $ 499.00

ProBlock™ Gold Mammalian Protease Inhibitor Cocktail [100X]

Catalog ID Size Pricing
GB-331-1 1 mL $ 70.00
GB-331-5 5 mL $ 252.00

Description

ProBlock™ Gold mammalian protease inhibitor cocktail contains optimized concentrations of various mammalian protease inhibitors, which provide excellent inhibition of protease activities during protein purification from mammalian cells and tissues. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine and specific inhibitors for mammalian proteases, such as aminopeptidases. A 1X concentration of ProBlock™ Gold Mammalian inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Mammalian is supplied additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

Product Specifications

Catalog ID GB-331
Storage/Handling Store at 4°C.
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GB-331-1 1 mL $ 70.00
GB-331-5 5 mL $ 252.00

ProBlock™ Gold Plant Protease Inhibitor Cocktail [100X]

Catalog ID Size Pricing
GB-332-1 1 mL $ 70.00
GB-332-5 5 mL $ 252.00

Description

ProBlock™ Gold Plant protease inhibitor cocktail contains optimized concentrations of various plant protease inhibitors, which provide excellent inhibition of protease activities during protein purification from plant. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for plant proteases, such as aspartic proteases and aminopeptidases. A 1X concentration oft ProBlock™ Gold Plant inhibits over 90% of protease activities.

Product Specifications

Catalog ID GB-332
Storage/Handling Store at 4°C.
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GB-332-1 1 mL $ 70.00
GB-332-5 5 mL $ 252.00

ProBlock™ Gold Protease Inhibitor Cocktail [100X]

Catalog ID Size Pricing
GB-108-2 2 mL $ 103.00
GB-108-5 5 mL $ 211.00
GB-108-10 10 mL $ 370.00

Description

Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins during purification from animal tissues, plant tissues, yeast and bacteria. ProBlock™ Gold contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. An separate bottle of EDTA is provided to inhibit metalloproteases.

Since some proteins require divalent cations like Ca2+, Mg2+ or Mn2+ for their biological activity, the presence of EDTA may be detrimental to the protein activity. Furthermore, if the protein of interest is purified using immobilized metal chelate affinity chromatography, EDTA must be removed from the buffer before the chromatography. The ProBlock™ Gold is therefore supplied with a separate, optional EDTA solution which may be added to the extraction buffer or lysate as needed.

Product Specifications

Catalog ID GB-108
Storage/Handling Store at 4°C.
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GB-108-2 2 mL $ 103.00
GB-108-5 5 mL $ 211.00
GB-108-10 10 mL $ 370.00

ProBlock™ Gold Yeast/Fungi Protease Inhibitor Cocktail [100X]

Catalog ID Size Pricing
GB-333-1 1 mL $ 70.00
GB-333-5 5 mL $ 252.00

Description

ProBlock™ Gold Yeast/Fungi protease inhibitor cocktail contains optimized concentrations of various yeast and fungal protease inhibitors, which provide excellent inhibition of protease activities during protein purification from yeast or fungi. The cocktail contains both reversible and irreversible protease inhibitors to inhibit serine, cysteine, metalloproteases and specific inhibitors for yeast or fungal proteases, such as aspartic proteases. At 1X concentration, ProBlock™ Gold Yeast/Fungi inhibits over 90% of protease activities.

Some proteins require divalent cations (Ca2+, Mg2+ or Mn2+) for their biological activity and the presence of chelating agents may be detrimental to the protein’s activity. Chelators, such as EDTA, inhibit the purification of proteins by immobilized metal affinity chromatography.

ProBlock™ Gold Yeast/Fungi is supplied additional, optional EDTA and 1,10-Phenanthroline monohydrate which are provided for optimal inhibition of metalloproteases and may be added in the extraction buffer or lysate as needed.

Product Specifications

Catalog ID GB-333
Storage/Handling Store at 4°C.
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GB-333-1 1 mL $ 70.00
GB-333-5 5 mL $ 252.00

ProBlock™ Protease Inhibitor Cocktail -100, EDTA free

Catalog ID Size Pricing
GB-328-100 10 x 10 Vial $ 458.00

Description

ProBlock™-100, EDTA Free is designed for large volumes of extraction/ lysis buffers and is supplied as 10 vials /kit, with each vial suitable for 100 ml.

ProBlock-100, EDTA Free protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail is EDTA free and contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

Product Specifications

Catalog ID GB-328
Storage/Handling Store at 4°C.
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GB-328-100 10 x 10 Vial $ 458.00

ProBlock™ Protease Inhibitor Cocktail -100, Plus EDTA

Catalog ID Size Pricing
GB-336-100 10 Vials $ 458.00

Description

ProBlock™-100, Plus EDTA is designed for large volumes of extraction/ lysis buffers and is supplied as 10 vials /kit, with each vial suitable for 100 ml.

ProBlock™-100, Plus EDTA protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is packed separately for inhibiting metalloproteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

Product Specifications

Catalog ID GB-336
Storage/Handling Store at 4°C.
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GB-336-100 10 Vials $ 458.00

ProBlock™ Protease Inhibitor Cocktail -50, EDTA free

Catalog ID Size Pricing
GB-326-1 1 Vial $ 62.00
GB-326-10 10 x 1 Vial $ 235.00
GB-326-20 20 x 1 Vial $ 461.00

Description

ProBlock™-50, EDTA Free is designed for large volumes of extraction/lysis buffers and is supplied as 1 vial/kit (Cat# GB-326-1), 10 vials /kit (Cat# GB-326-10) or 20 vials/kit (Cat# GB-326-20), with each vial suitable for 50 ml.

ProBlock-50, EDTA Free protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail is EDTA free and contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

Product Specifications

Catalog ID GB-326
Storage/Handling Store at 4°C.
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GB-326-1 1 Vial $ 62.00
GB-326-10 10 x 1 Vial $ 235.00
GB-326-20 20 x 1 Vial $ 461.00

ProBlock™ Protease Inhibitor Cocktail -50, Plus EDTA

Catalog ID Size Pricing
GB-334-10 10 Vials $ 235.00
GB-334-20 20 Vials $ 461.00

Description

ProBlock™-50, Plus EDTA is designed for large volumes of extraction/lysis buffers and is supplied as 10 vials /kit (Cat# GB-334-10) or 20 vials/kit (Cat# GB-334-20), with each vial suitable for 50 ml sample. EDTA [0.5M, 100X] is also supplied with this kit as an optional metalloprotease inhibitor.

ProBlock™-50, Plus EDTA protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is packed separately for inhibiting metalloproteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

Product Specifications

Catalog ID GB-334
Storage/Handling Store at 4°C.
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GB-334-10 10 Vials $ 235.00
GB-334-20 20 Vials $ 461.00

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