Protein A

Gold Biotechnology offers Protein A agarose resins for antibody purification. Protein A is a cell wall component of Staphylococcus aureus and consists of a cylindrical, single polypeptide chain that contains 5 antibody-binding domains. These high affinity regions specifically bind to Fc regions of immunoglobulins of various species. Protein A resins have been widely used to purify immunoglobulins of different mammalian species and also IgG subclasses, which have no specific affinity. Our Protein A resins are temperature stable, retain their native conformation even in the presence of denaturing agents, have a high IgG-binding capacity and binding stability and benefit from milder elution conditions than Protein G agarose resin. Protein A is ideally recommended for applications involving various human IgG (excluding IgG3) and mouse IgG2 as well as IgG from pigs, guinea pigs and rabbits.

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Protein A Agarose Resin (Pharmaceutical grade for research)

Catalog ID Size Pricing
P-400-5 5 mL $ 167.00
P-400-25 25 mL $ 651.00
P-400-50 50 mL $ 1,199.00
P-400-100 100 mL $ 2,054.00

Description

Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.

Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).

Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).

Product Specifications

Catalog ID P-400
Storage/Handling store at 4°C. Do NOT freeze.
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P-400-5 5 mL $ 167.00
P-400-25 25 mL $ 651.00
P-400-50 50 mL $ 1,199.00
P-400-100 100 mL $ 2,054.00

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