Gold Biotechnology offers Protein A agarose resins for antibody purification. Protein A is a cell wall component of Staphylococcus aureus and consists of a cylindrical, single polypeptide chain that contains 5 antibody-binding domains. These high affinity regions specifically bind to Fc regions of immunoglobulins of various species. Protein A resins have been widely used to purify immunoglobulins of different mammalian species and also IgG subclasses, which have no specific affinity. Our Protein A resins are temperature stable, retain their native conformation even in the presence of denaturing agents, have a high IgG-binding capacity and binding stability and benefit from milder elution conditions than Protein G agarose resin. Protein A is ideally recommended for applications involving various human IgG (excluding IgG3) and mouse IgG2 as well as IgG from pigs, guinea pigs and rabbits.
|P-400-5||5 mL||$ 178.00|
|P-400-25||25 mL||$ 691.00|
|P-400-50||50 mL||$ 1,271.00|
|P-400-100||100 mL||$ 2,178.00|
Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.
Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).
Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).
|Storage/Handling||store at 4°C. Do NOT freeze.|