Protein Expression & Purification

Proteins are fundamental macromolecules found in every biological system and form the basis of many fields of work from research into drug creation to industrial applications like fermentation. GoldBio protein products are ideal for every step of your protein synthesis. Our reagents and tools are of the highest quality available at a fraction of the prices of other vendors, so you can be assured that your research yields confident results without compromising your budget.
Showing 21-40 of 122 items.

Cobalt Agarose Beads (High Density)

Catalog ID Size Pricing
H-310-5 5 mL $ 45.00
H-310-10 10 mL $ 79.00
H-310-25 25 mL $ 179.00
H-310-50 50 mL $ 298.00
H-310-100 100 mL $ 546.00
H-310-500 500 mL $ 2,184.00
H-310-C8 Column: 8 x 1 mL $ 157.00
H-310-C5 Column: 5 x 5 mL $ 466.00

Description

Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.

Product Specifications

Catalog ID H-310
Storage/Handling store at 4°C. Do NOT freeze.
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H-310-5 5 mL $ 45.00
H-310-10 10 mL $ 79.00
H-310-25 25 mL $ 179.00
H-310-50 50 mL $ 298.00
H-310-100 100 mL $ 546.00
H-310-500 500 mL $ 2,184.00
H-310-C8 Column: 8 x 1 mL $ 157.00
H-310-C5 Column: 5 x 5 mL $ 466.00

Cobalt HTC Agarose Beads

Catalog ID Size Pricing
R-203-5 5 mL $ 64.00
R-203-25 25 mL $ 272.00
R-203-100 100 mL $ 685.00
R-203-500 500 mL $ 2,788.00

Description

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

Product Specifications

Catalog ID R-203
Storage/Handling store at 4°C. Do NOT freeze.
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R-203-5 5 mL $ 64.00
R-203-25 25 mL $ 272.00
R-203-100 100 mL $ 685.00
R-203-500 500 mL $ 2,788.00

Imidazole, Recrystallized

Catalog ID Size Pricing
I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-902
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at room temperature.
UN Number 3262
Item Class 8
Group Number III
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I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

Digitonin Special Grade (water soluble)

Catalog ID Size Pricing
D-180-250 250 mg $ 98.00
D-180-1 1 g $ 243.00
D-180-2.5 2.5 g $ 508.00
D-180-5 5 g $ 939.00
D-180-10 10 g $ 1,734.00

Description

Product Specifications

Catalog ID D-180
CAS # 11024-24-1
MW 1229.31 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
UN Number 2811
Item Class 6.1
Group Number II
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D-180-250 250 mg $ 98.00
D-180-1 1 g $ 243.00
D-180-2.5 2.5 g $ 508.00
D-180-5 5 g $ 939.00
D-180-10 10 g $ 1,734.00

Phenylmethylsulfonyl fluoride (PMSF)

Catalog ID Size Pricing
P-470-10 10 g $ 53.00
P-470-25 25 g $ 98.00
P-470-50 50 g $ 187.00
P-470-100 100 g $ 282.00

Description

PMSF is a serine protease inhibitor that irreversibly inhibits serine proteases by sulfonylation of the serine residue in the active site of the protease. It is widely used in protocols for the isolation of proteins.

PMSF is inactivated in aqueous solutions. The rate of inactivation increases with increasing pH and is faster at 25°C than at 4°C. The half-life of a 20mM aqueous solution of PMSF is ~35 minutes at pH 8.0. This short half-life means that aqueous solutions of PMSF can be safely discarded after they have been rendered alkaline (pH >8.6) and stored for several hours at room temperature.

Product Specifications

Catalog ID P-470
CAS # 329-98-6
MW 174.19 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at 4°C. Protect from light.
UN Number 2923
Item Class 8 (6.1)
Group Number II
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P-470-10 10 g $ 53.00
P-470-25 25 g $ 98.00
P-470-50 50 g $ 187.00
P-470-100 100 g $ 282.00

DTT (Dithiothreitol) (> 99% pure) Protease free

Catalog ID Size Pricing
DTT10 10 g $ 49.00
DTT25 25 g $ 109.00
DTT50 50 g $ 198.00
DTT100 100 g $ 319.00
DTT500 500 g $ 1,425.00

Description

Dithiothreitol (DTT) is the common name of the more popular of the two Cleland’s Reagents (the other being Dithioerythritol or DTE). DTT is a powerful reducing agent that forms a stable six-membered ring with an internal disulfide bond which is resistant to oxidation. DTT is often used for the following: reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl) cystamine to break apart the matrix of a polyacrylamide gel, the reduction of disulfide bonds in proteins, the prevention of those bonds from forming between cysteine residues and lastly, DTT is often used to reduce thiolated DNA in order to minimize dimerization.

DTT is a nearly 7-fold stronger reduction agent than βME (β-mercaptoethanol) and has a less offensive odor and is less toxic.

Product Specifications

Catalog ID DTT
CAS # 27565-41-9 / 3483-12-3
MW 154.25 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
UN Number 3335
Item Class 9
Group Number III
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DTT10 10 g $ 49.00
DTT25 25 g $ 109.00
DTT50 50 g $ 198.00
DTT100 100 g $ 319.00
DTT500 500 g $ 1,425.00

Gentamicin Sulfate, USP Grade

Catalog ID Size Pricing
G-400-1 1 g $ 23.00
G-400-5 5 g $ 34.00
G-400-10 10 g $ 58.00
G-400-25 25 g $ 88.00
G-400-100 100 g $ 282.00

Description

Gentamicin is an aminoglycoside, broad-spectrum antibiotic that effectively inhibits Gram-negative and Gram-positive cell growth.  Gentamicin's mode of action is to bind to the 30S ribosomal subunit and interfere with protein synthesis.

It is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against gram-positive and gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options for infected patients.

Product Specifications

Catalog ID G-400
CAS # 1405-41-0
MW 575.67 g/mol
Storage/Handling Store desiccated at -20°C.
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G-400-1 1 g $ 23.00
G-400-5 5 g $ 34.00
G-400-10 10 g $ 58.00
G-400-25 25 g $ 88.00
G-400-100 100 g $ 282.00

Glutathione Agarose Resin

Catalog ID Size Pricing
G-250-5 5 mL $ 84.00
G-250-10 10 mL $ 158.00
G-250-50 50 mL $ 610.00
G-250-100 100 mL $ 953.00

Description

Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.

Product Specifications

Catalog ID G-250
Storage/Handling store at 4°C. Do NOT freeze.
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G-250-5 5 mL $ 84.00
G-250-10 10 mL $ 158.00
G-250-50 50 mL $ 610.00
G-250-100 100 mL $ 953.00

IPTG

Catalog ID Size Pricing
I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00   $ 22.00
I2481C25 25 g $ 115.00   $ 92.00
I2481C50 50 g $ 184.00   $ 148.00
I2481C100 100 g $ 349.00   $ 280.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

Product Specifications

Catalog ID I2481
CAS # 367-93-1
MW 238.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00
$ 22.00
I2481C25 25 g $ 92.00
I2481C50 50 g $ 184.00
$ 148.00
I2481C100 100 g $ 349.00
$ 280.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Kanamycin Monosulfate, USP Grade

Catalog ID Size Pricing
K-120-5 5 g $ 23.00
K-120-10 10 g $ 44.00
K-120-25 25 g $ 92.00
K-120-50 50 g $ 173.00
K-120-100 100 g $ 323.00
K-120-250 250 g $ 748.00

Description

Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus. It is effective against gram-positive and gram-negative bacteria as well as Mycoplasma species. Kanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have the NPT II (APH3) gene incorporated in them. Kanamycin is very soluble in aqueous solution and is typically used in concentrations of 50-100 µg/ml.

Product Specifications

Catalog ID K-120
CAS # 25389-94-0
MW 582.58 g/mol
Storage/Handling Store desiccated at -20°C.
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K-120-5 5 g $ 23.00
K-120-10 10 g $ 44.00
K-120-25 25 g $ 92.00
K-120-50 50 g $ 173.00
K-120-100 100 g $ 323.00
K-120-250 250 g $ 748.00

Low Melt Agarose

Catalog ID Size Pricing
A-204-25 25 g $ 119.00
A-204-100 100 g $ 257.00

Description

For applications requiring low gel/melt temperature.
Low Melting (LM) Agarose is a low melting temperature agarose with the highest resolving capacity for large DNA fragments, including PCR products.
Low melting temperature allows for the recovery of undamaged nucleic acids below denaturation temperature. Low gelling temperature ensures In-Gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps. 

Low Melting Agarose is ideal for digestion by agarose enzymes, which makes it very easy to recover large DNA fragments suitable for cloning or enzymatic processing.

Product Specifications

Catalog ID A-204
CAS # 9012-36-6
Storage/Handling Store at room temperature.
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A-204-25 25 g $ 119.00
A-204-100 100 g $ 257.00

Macerozyme R-10

There is no image for Macerozyme R-10 Macerozyme R-10
Catalog ID Size Pricing
M8002.0001 1 g $ 83.00
M8002.0005 5 g $ 162.00
M8002.0010 10 g $ 253.00

Description

No description available

Product Specifications

Catalog ID M8002
CAS # 9032-75-1
Storage/Handling Store at 4°C.
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M8002.0001 1 g $ 83.00
M8002.0005 5 g $ 162.00
M8002.0010 10 g $ 253.00

n-Dodecyl-B-D-maltoside (DDM)

Catalog ID Size Pricing
DDM500 500 mg $ 31.00
DDM5 5 g $ 126.00
DDM10 10 g $ 241.00
DDM25 25 g $ 573.00

Description

No description available

Product Specifications

Catalog ID DDM
CAS # 69227-93-6
MW 510.63 g/mol
Storage/Handling Store at -20°C. Protect from light.
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DDM500 500 mg $ 31.00
DDM5 5 g $ 126.00
DDM10 10 g $ 241.00
DDM25 25 g $ 573.00

Nickel Agarose Beads (High Density)

Catalog ID Size Pricing
H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-C5 Column: 5x5 mL $ 466.00

Description

Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-320
Storage/Handling store at 4°C. Do NOT freeze.
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H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-C5 Column: 5x5 mL $ 466.00

Nickel HTC Agarose Beads

Catalog ID Size Pricing
R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Description

Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID R-202
Storage/Handling store at 4°C. Do NOT freeze.
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R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Nitrotetrazolium blue chloride (NBT)

Catalog ID Size Pricing
NBT1 1 g $ 48.00
NBT2.5 2.5 g $ 98.00
NBT5 5 g $ 158.00
NBT10 10 g $ 254.00

Description

Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple-brown final color of the precipitates at the reaction sites.

NBT has also been used with X-Gal,or Salmon-Gal to speed up and enhance colony screening.

Product Specifications

Catalog ID NBT
CAS # 298-83-9
MW 817.65 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C. Protect from light.
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NBT1 1 g $ 48.00
NBT2.5 2.5 g $ 98.00
NBT5 5 g $ 158.00
NBT10 10 g $ 254.00

Octylglucoside, Ultra Pure

Catalog ID Size Pricing
O-110-5 5 g $ 92.00
O-110-10 10 g $ 166.00
O-110-25 25 g $ 255.00
O-110-50 50 g $ 486.00

Description

Octylglucoside is a nonionic, mild detergent used to isolate membrane proteins in the native state. It can easily be removed during final extractions by dialysis.

Characteristics that contribute to this detergent’s usefulness are its water solubility, chemical structure and small-sized micelles.

Octylglucoside also provides higher resolution of proteins in 2D electrophoresis.

Product Specifications

Catalog ID O-110
CAS # 29836-26-8
MW 292.38 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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O-110-5 5 g $ 92.00
O-110-10 10 g $ 166.00
O-110-25 25 g $ 255.00
O-110-50 50 g $ 486.00

Octylthiogalactoside

Catalog ID Size Pricing
O-120-5 5 g $ 220.00
O-120-10 10 g $ 408.00
O-120-25 25 g $ 942.00
O-120-50 50 g $ 1,742.00

Description

No description available

Product Specifications

Catalog ID O-120
CAS # 42891-16-7
MW 308.43 g/mol
Grade MOLECULAR BIOLOGY GRADE
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O-120-5 5 g $ 220.00
O-120-10 10 g $ 408.00
O-120-25 25 g $ 942.00
O-120-50 50 g $ 1,742.00

Pectolyase Y-23

Catalog ID Size Pricing
P8004.0001 1 g $ 367.00
P8004.0005 5 g $ 1,449.00

Description

No description available

Product Specifications

Catalog ID P8004
CAS # 9033-35-6
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P8004.0001 1 g $ 367.00
P8004.0005 5 g $ 1,449.00

ProBlock™ Protease Inhibitor Cocktail -50, EDTA free

Catalog ID Size Pricing
GB-326-1 1 mL $ 62.00
GB-326-10 10 x 1 mL $ 235.00
GB-326-20 20 x 1 mL $ 461.00

Description

ProBlock™-50, EDTA Free is designed for large volumes of extraction/lysis buffers and is supplied as 1 vial/kit (Cat# GB-326-1), 10 vials /kit (Cat# GB-326-10) or 20 vials/kit (Cat# GB-326-20), with each vial suitable for 50 ml.

ProBlock-50, EDTA Free protease inhibitor cocktail contains optimized concentrations of various protease inhibitors, which provide excellent inhibition of protease activities during protein purification. ProBlock™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail is EDTA free and contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. At 1X concentration, ProBlock™ inhibits over 90% of protease activities (e.g. Mouse Pancreas Extract 0.5 mg/ml protein).

Product Specifications

Catalog ID GB-326
Storage/Handling Store at 4°C.
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GB-326-1 1 mL $ 62.00
GB-326-10 10 x 1 mL $ 235.00
GB-326-20 20 x 1 mL $ 461.00

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