Protein Expression & Purification

Proteins are fundamental macromolecules found in every biological system and form the basis of many fields of work from research into drug creation to industrial applications like fermentation. GoldBio protein products are ideal for every step of your protein synthesis. Our reagents and tools are of the highest quality available at a fraction of the prices of other vendors, so you can be assured that your research yields confident results without compromising your budget.
Showing 61-80 of 122 items.

IPTG

Catalog ID Size Pricing
I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00   $ 22.00
I2481C25 25 g $ 115.00   $ 92.00
I2481C50 50 g $ 184.00   $ 148.00
I2481C100 100 g $ 349.00   $ 280.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

Product Specifications

Catalog ID I2481
CAS # 367-93-1
MW 238.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00
$ 22.00
I2481C25 25 g $ 92.00
I2481C50 50 g $ 184.00
$ 148.00
I2481C100 100 g $ 349.00
$ 280.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

IPTG EZ Pak™ for 1 M Solution

Catalog ID Size Pricing
I2481-EZ10 10 mL $ 58.00
I2481-EZ50 50 mL $ 92.00
I2481-EZ100 100 mL $ 150.00

Description

The IPTG EZ Pak™ is the fastest and easiest way to make a set amount of sterile IPTG solution. The kit includes preweighed IPTG powder, a sterile filter, and a sterile container for the filtered solution. No need to calculate, simply add the stated amount of deionized H2O, filter, and pour into the labeled bottle for easy usage. The EZ Pak™ includes high quality GoldBio IPTG and the sterile solution is ready for tissue culture, bacterial media, or any other appropriate use.

Product Specifications

Catalog ID I2481-EZ
CAS # 367-93-1
MW 238.30 g/mol
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I2481-EZ10 10 mL $ 58.00
I2481-EZ50 50 mL $ 92.00
I2481-EZ100 100 mL $ 150.00

Ixazomib Citrate

Catalog ID Size Pricing
I-350-10 10 mg $ 157.00

Description

No description available

Product Specifications

Catalog ID I-350
CAS # 1239908-20-3
MW 517.12 g/mol
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I-350-10 10 mg $ 157.00

Kanamycin Monosulfate, USP Grade

Catalog ID Size Pricing
K-120-5 5 g $ 23.00
K-120-10 10 g $ 44.00
K-120-25 25 g $ 92.00
K-120-50 50 g $ 173.00
K-120-100 100 g $ 323.00
K-120-250 250 g $ 748.00

Description

Kanamycin is an aminoglycoside antibiotic isolated from Streptomyces kanamyceticus. It is effective against gram-positive and gram-negative bacteria as well as Mycoplasma species. Kanamycin is commonly used to select for bacteria that have been transformed with a kanamycin-resistant gene and plant tissues that have the NPT II (APH3) gene incorporated in them. Kanamycin is very soluble in aqueous solution and is typically used in concentrations of 50-100 µg/ml.

Product Specifications

Catalog ID K-120
CAS # 25389-94-0
MW 582.58 g/mol
Storage/Handling Store desiccated at -20°C.
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K-120-5 5 g $ 23.00
K-120-10 10 g $ 44.00
K-120-25 25 g $ 92.00
K-120-50 50 g $ 173.00
K-120-100 100 g $ 323.00
K-120-250 250 g $ 748.00

L-Glutathione, Reduced

Catalog ID Size Pricing
G-155-25 25 g $ 77.00
G-155-50 50 g $ 141.00
G-155-100 100 g $ 251.00
G-155-250 250 g $ 436.00
G-155-500 500 g $ 819.00
G-155-1000 1 kg $ 1,528.00

Description

L-Glutathione is a co-substrate to glutathione transferase found in plants and animals and is involved in the elimination of free radicals resulting from both cellular respiratory and metabolic activities.

Product Specifications

Catalog ID G-155
CAS # 70-18-8
MW 307.32 g/mol
Storage/Handling Store at 4°C. Protect from light.
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G-155-25 25 g $ 77.00
G-155-50 50 g $ 141.00
G-155-100 100 g $ 251.00
G-155-250 250 g $ 436.00
G-155-500 500 g $ 819.00
G-155-1000 1 kg $ 1,528.00

Leupeptin Hemisulfate

Catalog ID Size Pricing
L-010-5 5 mg $ 42.00
L-010-25 25 mg $ 84.00
L-010-100 100 mg $ 275.00

Description

No description available

Product Specifications

Catalog ID L-010
CAS # 103476-89-7
MW 475.59 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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L-010-5 5 mg $ 42.00
L-010-25 25 mg $ 84.00
L-010-100 100 mg $ 275.00

Low Melt Agarose

Catalog ID Size Pricing
A-204-25 25 g $ 119.00
A-204-100 100 g $ 257.00

Description

For applications requiring low gel/melt temperature.
Low Melting (LM) Agarose is a low melting temperature agarose with the highest resolving capacity for large DNA fragments, including PCR products.
Low melting temperature allows for the recovery of undamaged nucleic acids below denaturation temperature. Low gelling temperature ensures In-Gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps. 

Low Melting Agarose is ideal for digestion by agarose enzymes, which makes it very easy to recover large DNA fragments suitable for cloning or enzymatic processing.

Product Specifications

Catalog ID A-204
CAS # 9012-36-6
Storage/Handling Store at room temperature.
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A-204-25 25 g $ 119.00
A-204-100 100 g $ 257.00

Lysozyme, Egg White

There is no image for Lysozyme, Egg White Lysozyme, Egg White
Catalog ID Size Pricing
L-040-1 1 g $ 28.00
L-040-5 5 g $ 44.00
L-040-10 10 g $ 61.00
L-040-25 25 g $ 109.00
L-040-100 100 g $ 218.00
L-040-250 250 g $ 502.00

Description

Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.

Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.

Product Specifications

Catalog ID L-040
CAS # 12650-88-3
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
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L-040-1 1 g $ 28.00
L-040-5 5 g $ 44.00
L-040-10 10 g $ 61.00
L-040-25 25 g $ 109.00
L-040-100 100 g $ 218.00
L-040-250 250 g $ 502.00

Macerozyme R-10

There is no image for Macerozyme R-10 Macerozyme R-10
Catalog ID Size Pricing
M8002.0001 1 g $ 83.00
M8002.0005 5 g $ 162.00
M8002.0010 10 g $ 253.00

Description

No description available

Product Specifications

Catalog ID M8002
CAS # 9032-75-1
Storage/Handling Store at 4°C.
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M8002.0001 1 g $ 83.00
M8002.0005 5 g $ 162.00
M8002.0010 10 g $ 253.00

Mammalian Cell Lysis Buffer

Catalog ID Size Pricing
GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

Description

Product Specifications

Catalog ID GB-180
Storage/Handling Store at 4°C.
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GB-180-100 100 mL $ 58.00
GB-180-500 500 mL $ 117.00

n-Dodecyl-B-D-maltoside (DDM)

Catalog ID Size Pricing
DDM500 500 mg $ 31.00
DDM5 5 g $ 126.00
DDM10 10 g $ 241.00
DDM25 25 g $ 573.00

Description

No description available

Product Specifications

Catalog ID DDM
CAS # 69227-93-6
MW 510.63 g/mol
Storage/Handling Store at -20°C. Protect from light.
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DDM500 500 mg $ 31.00
DDM5 5 g $ 126.00
DDM10 10 g $ 241.00
DDM25 25 g $ 573.00

NDSB-201 (Non-detergent sulfobetaine)

Catalog ID Size Pricing
N-201-5 5 g $ 21.00
N-201-25 25 g $ 24.00
N-201-50 50 g $ 34.00
N-201-100 100 g $ 52.00
N-201-250 250 g $ 100.00

Description

No description available

Product Specifications

Catalog ID N-201
CAS # 15471-17-7
MW 201.24 g/mol
Storage/Handling Store desiccated at room temperature.
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N-201-5 5 g $ 21.00
N-201-25 25 g $ 24.00
N-201-50 50 g $ 34.00
N-201-100 100 g $ 52.00
N-201-250 250 g $ 100.00

Nickel Agarose Beads (High Density)

Catalog ID Size Pricing
H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-C5 Column: 5x5 mL $ 466.00

Description

Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-320
Storage/Handling store at 4°C. Do NOT freeze.
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H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-C5 Column: 5x5 mL $ 466.00

Nickel Agarose Resin Cartridges (High Density)

Catalog ID Size Pricing
H-322-CA1 Cartridge: 1x5 mL $ 121.00
H-322-CA5 Cartridge: 5x5 mL $ 473.00

Description

Nickel Agarose Resin is available in a prepacked cartridge, specifically for use in ÄKTA or Profinia protein purification systems (see additional information for compatible systems).

It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-322
Storage/Handling store at 4°C. Do NOT freeze.
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H-322-CA1 Cartridge: 1x5 mL $ 121.00
H-322-CA5 Cartridge: 5x5 mL $ 473.00

Nickel HTC Agarose Beads

Catalog ID Size Pricing
R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Description

Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID R-202
Storage/Handling store at 4°C. Do NOT freeze.
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R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Nickel NTA Agarose Beads

There is no image for Nickel NTA Agarose Beads Nickel NTA Agarose Beads
Catalog ID Size Pricing
H-350-5 5 mL $ 59.00
H-350-10 10 mL $ 95.00
H-350-25 25 mL $ 207.00
H-350-50 50 mL $ 359.00
H-350-100 100 mL $ 678.00

Description

No description available

Product Specifications

Catalog ID H-350
Storage/Handling store at 4°C. Do NOT freeze.
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H-350-5 5 mL $ 59.00
H-350-10 10 mL $ 95.00
H-350-25 25 mL $ 207.00
H-350-50 50 mL $ 359.00
H-350-100 100 mL $ 678.00

Nickel NTA Magnetic Agarose Beads

There is no image for Nickel NTA Magnetic Agarose Beads Nickel NTA Magnetic Agarose Beads
Catalog ID Size Pricing
H-351-2 2 mL $ 97.00
H-351-5 5 mL $ 218.00
H-351-10 10 mL $ 396.00

Description

No description available

Product Specifications

Catalog ID H-351
Storage/Handling store at 4°C. Do NOT freeze.
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H-351-2 2 mL $ 97.00
H-351-5 5 mL $ 218.00
H-351-10 10 mL $ 396.00

Nitrotetrazolium blue chloride (NBT)

Catalog ID Size Pricing
NBT1 1 g $ 48.00
NBT2.5 2.5 g $ 98.00
NBT5 5 g $ 158.00
NBT10 10 g $ 254.00

Description

Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple-brown final color of the precipitates at the reaction sites.

NBT has also been used with X-Gal,or Salmon-Gal to speed up and enhance colony screening.

Product Specifications

Catalog ID NBT
CAS # 298-83-9
MW 817.65 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C. Protect from light.
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NBT1 1 g $ 48.00
NBT2.5 2.5 g $ 98.00
NBT5 5 g $ 158.00
NBT10 10 g $ 254.00

Octylglucoside, Ultra Pure

Catalog ID Size Pricing
O-110-5 5 g $ 92.00
O-110-10 10 g $ 166.00
O-110-25 25 g $ 255.00
O-110-50 50 g $ 486.00

Description

Octylglucoside is a nonionic, mild detergent used to isolate membrane proteins in the native state. It can easily be removed during final extractions by dialysis.

Characteristics that contribute to this detergent’s usefulness are its water solubility, chemical structure and small-sized micelles.

Octylglucoside also provides higher resolution of proteins in 2D electrophoresis.

Product Specifications

Catalog ID O-110
CAS # 29836-26-8
MW 292.38 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
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O-110-5 5 g $ 92.00
O-110-10 10 g $ 166.00
O-110-25 25 g $ 255.00
O-110-50 50 g $ 486.00

Octylthiogalactoside

Catalog ID Size Pricing
O-120-5 5 g $ 220.00
O-120-10 10 g $ 408.00
O-120-25 25 g $ 942.00
O-120-50 50 g $ 1,742.00

Description

No description available

Product Specifications

Catalog ID O-120
CAS # 42891-16-7
MW 308.43 g/mol
Grade MOLECULAR BIOLOGY GRADE
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O-120-5 5 g $ 220.00
O-120-10 10 g $ 408.00
O-120-25 25 g $ 942.00
O-120-50 50 g $ 1,742.00

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