Protein Gel Staining
Detection of proteins immobilized in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is an essential step in Western blots and protein analysis. GoldBio offers a variety of easy-to-use in-gel dye-based stains that diffuse into the gel allowing you to visualize your protein and accurately perform protein quantification. Our Coomassie Brilliant Blue G-250 and R250 dyes produce a robust signal in SDS-PAGE and blue native (BN-PAGE) and are compatible with downstream applications including protein extraction, sequencing and mass spectrometry. We also carry a safer and faster alternative to Coomassie stains, Blazin’ Blue™ Protein Gel Stain, which allows detection of protein through visible blue staining or by near-infrared fluorescence. Alternatively, you can detect your protein with our highly sensitive Blazin’ Bright™ Luminescent Protein Gel Stains, which can stain as little as 1-10 ng of protein in a single step and without the need for fixation.
Blazin' Blue™ Protein Gel Stain
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-810-1 | 1 L | $ 127.00 |
Description
Blazin' Blue™ Protein Gel Stain is a ready-to-use protein gel staining solution that is a safer and more effective replacement for traditional Coomassie stains. Blazin' Blue™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation or washing. Protein bands can be detected through visible blue staining or by near-infrared fluorescence. And since Blazin' Blue™ is aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Blue™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.
Product Specifications
| Catalog ID | P-810 |
|---|---|
| Storage/Handling | Store at 4°C. |
Blazin' Bright™ Luminescent Protein Gel Stain
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-820-1 | 1 L | $ 166.00 |
Description
Blazin' Bright™ Luminescent Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Blazin' Bright™ offers a faster alternative to protein staining (5-60 minutes) in a single step without the need for fixation (destaining is optional). The Blazin' Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.
Blazin' Bright™ has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin' Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.
Product Specifications
| Catalog ID | P-820 |
|---|---|
| Storage/Handling | Store at room temperature. |
Blazin' Bright™ Luminescent UV Protein Gel Stain
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| P-825-1 | 1 L | $ 224.00 |
Description
Blazin' Bright™ Luminescent UV Protein Gel Stain is a ready-to-use luminescent protein gel staining solution that is a safer and more effective replacement for traditional fluorescent staining. Gels stained with our Blazin' Bright™ Luminescent UV Protein Gel Stain can be visualized rapidly (5-30 minutes) in a single step without fixation (destaining is optional). The Blazin' Bright™ gel stains are aqueous based, there are no hazardous chemicals like methanol or acetic acid to worry about. Blazin' Bright™ Protein Gel Stain is certified under CCR Title 22 as non-toxic to the environment for drain disposal after a simple pH neutralization step.
Blazin' Bright™ Luminescent UV has a detection limit around 1-10 ng, depending on the detection method used (staining intensity varies between proteins) and does not cause gel shrinkage. Blazin' Bright™ staining is also fully compatible with mass spectrometry (MS) and Edman-based sequencing.
Product Specifications
| Catalog ID | P-825 |
|---|---|
| Storage/Handling | Store at 4°C. |
Coomassie Brilliant Blue G-250
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| C-460-5 | 5 g | $ 29.00 | |
| C-460-25 | 25 g | $ 39.00 | |
| C-460-50 | 50 g | $ 75.00 |
Description
Coomassie Brilliant Blue G-250 (CBBG) is a member of the Coomassie family and is used as a common analytical dye in SDS-PAGE and BN-PAGE. CBBG can also be used with the Bradford Method to determine protein concentration.
Product Specifications
| Catalog ID | C-460 |
|---|---|
| CAS # | 6104-58-1 |
| MW | 854.02 g/mol |
| Storage/Handling | Store desiccated at room temperature. |
Coomassie Brilliant Blue R-250
| Catalog ID | Size | Pricing | |
|---|---|---|---|
| C-461-5 | 5 g | $ 39.00 | |
| C-461-25 | 25 g | $ 55.00 | |
| C-461-100 | 100 g | $ 89.00 |
Description
Coomassie Brilliant Blue R-250 (CBBR) is a member of the Coomassie family and is used extensively as an analytical dye in SDS-PAGE.
Product Specifications
| Catalog ID | C-461 |
|---|---|
| CAS # | 6104-59-2 |
| MW | 825.97 g/mol |
| Storage/Handling | Store desiccated at room temperature. |
