Protein Purification

Separating proteins from cellular components is critical when trying to determine their overall structure and function. GoldBio carries products designed for the whole range of chromatography applications, from gel chromatography to more targeted applications like affinity, ion exchange or polyhistidine-tag binding. When it comes to purification, the higher the quality of materials, the better the results. GoldBio agaroses come in different densities, with high throughput beads designed for industrial applications. Our Cobalt and Nickel agarose beads are perfect for your his-tag recombinant protein recovery, and our Ultra HBCTM streptavidin resin has superior coupling chemistry and higher binding performance than other vendors. GoldBio offers quality products, at affordable prices.
Showing 1-14 of 14 items.

Cobalt Agarose Beads (High Density)

Catalog ID Size Pricing
H-310-5 5 mL $ 45.00
H-310-10 10 mL $ 79.00
H-310-C8 Column: 8 x 1 mL $ 157.00
H-310-25 25 mL $ 179.00
H-310-50 50 mL $ 298.00
H-310-C5 Column: 5 x 5 mL $ 466.00
H-310-100 100 mL $ 546.00
H-310-500 500 mL $ 2,184.00

Description

Available in bulk or in prepacked columns, cobalt has a binding capacity of >25 mg/ml with approximately 60 kDa protein. Cobalt binds fewer host protein contaminants, resulting in lower background than nickel resins and no metal contamination in eluted histidine-tagged protein sample. In addition, it allows for purification of proteins under native or denaturing conditions. Cobalt Agarose works very well with Ni/Cu/Co Resin Wash Buffer and Histidine Protein Elution Buffer.

Product Specifications

Catalog ID H-310
Storage/Handling store at 4°C. Do NOT freeze.
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H-310-5 5 mL $ 45.00
H-310-10 10 mL $ 79.00
H-310-C8 Column: 8 x 1 mL $ 157.00
H-310-25 25 mL $ 179.00
H-310-50 50 mL $ 298.00
H-310-C5 Column: 5 x 5 mL $ 466.00
H-310-100 100 mL $ 546.00
H-310-500 500 mL $ 2,184.00

Cobalt HTC Agarose Beads

Catalog ID Size Pricing
R-203-5 5 mL $ 64.00
R-203-25 25 mL $ 272.00
R-203-100 100 mL $ 685.00
R-203-500 500 mL $ 2,788.00

Description

Cobalt HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Cobalt purification with the high flow rates of the HTC resin (70% higher than commercial standards).

Product Specifications

Catalog ID R-203
Storage/Handling store at 4°C. Do NOT freeze.
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R-203-5 5 mL $ 64.00
R-203-25 25 mL $ 272.00
R-203-100 100 mL $ 685.00
R-203-500 500 mL $ 2,788.00

Glutathione Agarose Resin

Catalog ID Size Pricing
G-250-5 5 mL $ 84.00
G-250-10 10 mL $ 158.00
G-250-50 50 mL $ 610.00
G-250-100 100 mL $ 953.00

Description

Glutathione has been covalently linked for use in affinity purification of glutathione-S-transferase (GST) and GST fusion proteins.

Product Specifications

Catalog ID G-250
Storage/Handling store at 4°C. Do NOT freeze.
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G-250-5 5 mL $ 84.00
G-250-10 10 mL $ 158.00
G-250-50 50 mL $ 610.00
G-250-100 100 mL $ 953.00

Imidazole - 3M Recrystallized Solution, pH 6.0

Catalog ID Size Pricing
I-901-25 25 mL $ 68.00
I-901-50 50 mL $ 101.00
I-901-100 100 mL $ 156.00
I-901-250 250 mL $ 361.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-901
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at 4°C.
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I-901-25 25 mL $ 68.00
I-901-50 50 mL $ 101.00
I-901-100 100 mL $ 156.00
I-901-250 250 mL $ 361.00

Imidazole, Recrystallized

Catalog ID Size Pricing
I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

Description

Imidazole is a planar 5-membered ring. It is a highly polar, highly water soluble amphoteric compound. Imidazole is often used to elute tagged proteins bound to metal ions attached to the surface of beads in a chromatography column by displacing the His-tag from the metal ion and freeing the His-tagged proteins.

Product Specifications

Catalog ID I-902
CAS # 288-32-4
MW 68.08 g/mol
Storage/Handling Store at room temperature.
UN Number 3262
Item Class 8
Group Number III
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I-902-25 25 g $ 68.00
I-902-50 50 g $ 115.00
I-902-100 100 g $ 184.00
I-902-500 500 g $ 562.00

NDSB-201 (Non-detergent sulfobetaine)

Catalog ID Size Pricing
N-201-5 5 g $ 21.00
N-201-25 25 g $ 24.00
N-201-50 50 g $ 34.00
N-201-100 100 g $ 52.00
N-201-250 250 g $ 100.00

Description

NDSB-201 is a protein specific additive that can be used to help solubilize and stabilize proteins at non-denaturing concentrations while preventing protein aggregation.

NDSB-201 is a member of a group of nondetergent sulfobetaines. These zwitterionic compounds can reduce aggregation and aid in refolding and renaturing of proteins found in bacterial expression systems and in inclusion bodies. Unlike detergents such as CHAPS, NDSBs are nondenaturing, even at higher concentrations and have been shown to increase the yield efficiency of membrane and nuclear proteins, among others.

Product Specifications

Catalog ID N-201
CAS # 15471-17-7
MW 201.24 g/mol
Storage/Handling Store desiccated at room temperature.
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N-201-5 5 g $ 21.00
N-201-25 25 g $ 24.00
N-201-50 50 g $ 34.00
N-201-100 100 g $ 52.00
N-201-250 250 g $ 100.00

Nickel Agarose Beads (High Density)

Catalog ID Size Pricing
H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-C5 Column: 5x5 mL $ 466.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00

Description

Nickel Agarose Resin is available in bulk or in prepacked columns. It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-320
Storage/Handling store at 4°C. Do NOT freeze.
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H-320-5 5 mL $ 45.00
H-320-10 10 mL $ 79.00
H-320-C8 Column: 8x1 mL $ 157.00
H-320-25 25 mL $ 179.00
H-320-50 50 mL $ 298.00
H-320-C5 Column: 5x5 mL $ 466.00
H-320-100 100 mL $ 546.00
H-320-500 500 mL $ 2,184.00

Nickel Agarose Resin Cartridges (High Density)

Catalog ID Size Pricing
H-322-CA1 Cartridge: 1x5 mL $ 121.00
H-322-CA5 Cartridge: 5x5 mL $ 473.00

Description

Nickel Agarose Resin is available in a prepacked cartridge, specifically for use in ÄKTA or Profinia protein purification systems (see additional information for compatible systems).

It has a binding capacity of >25 mg/ml with approximately 60 kDa protein. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

IDA cross-linked Agarose resin consists of iminodiacetic acid groups ligated by stable ether linkages via a spacer arm. IDA is a tridentate chelating agent, covalently coupled to cross-linked agarose beads. This resin is loaded with Ni2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Affinity Chromatography is based on the interaction between certain superficial protein residues (histidines, cysteines and to a lesser extent tryptophans), with transition metal cations, forming chelates.

Product Specifications

Catalog ID H-322
Storage/Handling store at 4°C. Do NOT freeze.
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H-322-CA1 Cartridge: 1x5 mL $ 121.00
H-322-CA5 Cartridge: 5x5 mL $ 473.00

Nickel HTC Agarose Beads

Catalog ID Size Pricing
R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Description

Nickel HTC 6% Agarose Beads are suitable for downstream purification using IMAC. These resins combine the advantages of Nickel purification with the high flow rates of the HTC resin (70% higher than commercial standards).

HTC beads are high throughput beads designed to meet the demand for industrial process separation. Their rigidity and mechanical resistance permits high flow rates, with good resolution in a minimum time frame, making these beads ideal for process-scale use. HTC beads are highly crosslinked 4% and 6% agarose matrices and have excellent physical and chromatographic qualities. They are autoclavable. These beads are an ideal support for the immobilization of ligands for Affinity Chromatography and base media support for producing IEX (Ion Exchange) and Hydrophobic interaction chromatography resins.

Product Specifications

Catalog ID R-202
Storage/Handling store at 4°C. Do NOT freeze.
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R-202-5 5 mL $ 64.00
R-202-25 25 mL $ 272.00
R-202-100 100 mL $ 685.00
R-202-500 500 mL $ 2,788.00
R-202-1000 1000 mL $ 5,016.00

Nickel NTA Agarose Beads

There is no image for Nickel NTA Agarose Beads Nickel NTA Agarose Beads
Catalog ID Size Pricing
H-350-5 5 mL $ 59.00
H-350-10 10 mL $ 95.00
H-350-25 25 mL $ 207.00
H-350-50 50 mL $ 359.00
H-350-100 100 mL $ 678.00

Description

Nickel-NTA Agarose Resin has a binding capacity of ~50 mg/ml. In addition, it allows for purification of proteins under native or denaturing conditions. GoldBio Nickel Agarose works very well with the His-Tag Buffer Set.

NTA cross-linked Agarose resin consists of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent, covalently coupled to cross-linked agarose beads, providing a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-350
Storage/Handling store at 4°C. Do NOT freeze.
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H-350-5 5 mL $ 59.00
H-350-10 10 mL $ 95.00
H-350-25 25 mL $ 207.00
H-350-50 50 mL $ 359.00
H-350-100 100 mL $ 678.00

Nickel NTA Magnetic Agarose Beads

There is no image for Nickel NTA Magnetic Agarose Beads Nickel NTA Magnetic Agarose Beads
Catalog ID Size Pricing
H-351-2 2 mL $ 97.00
H-351-5 5 mL $ 218.00
H-351-10 10 mL $ 396.00

Description

Nickel NTA Magnetic Agarose Beads are a rapid and easy small scale purification of histidine-tagged proteins. This resin consists of magnetic agarose derivatized with Nitrilotriacetic (NTA) and provides good properties working in native or denaturing conditions. Magnetic agarose beads provide a convenient and quick method for purification without the need for pipetting or centrifugation. Washing, binding and elution steps will require a magnetic device.

NTA cross-linked resins consist of nitrilotriacetic acid groups ligated by stable ether linkages via a spacer arm. NTA is a tetravalent chelating agent which provides a higher specificity and lower ion leaching than IDA linked resins. NTA resins have also been shown to be more robust in the presence of higher concentrations of EDTA, but may require a higher imidazole concentration for protein elution. This resin is loaded with Ni 2+. The resulting, ready-to-use resin is ideal for rapid purifications of His-tagged proteins.

Product Specifications

Catalog ID H-351
Storage/Handling store at 4°C. Do NOT freeze.
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H-351-2 2 mL $ 97.00
H-351-5 5 mL $ 218.00
H-351-10 10 mL $ 396.00

Protein A Agarose Resin (Pharmaceutical grade for research)

Catalog ID Size Pricing
P-400-5 5 mL $ 154.00
P-400-25 25 mL $ 604.00
P-400-50 50 mL $ 1,113.00
P-400-100 100 mL $ 1,907.00

Description

Protein A agarose resin consists of rProtein A covalently bound to cross-linked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein A allowing for reuse of the affinity resin in several purification steps. Protein A Agarose resin is available in bulk from 5-100 ml quantities.

Protein A resin is good for the purification of a wide range of Immunoglobulins of different mammalian species and also certain IgG subclasses that have no other affinity (see additional information).

Protein A is a cell wall component of Staphylococcus aureus. It consists of a single polypeptide chain shaped as a cylinder, which contains five antibody–binding domains. These high affinity regions are specifically bonded to Fc region of the immunoglobulins (specially IgGs of different species).

Product Specifications

Catalog ID P-400
Storage/Handling store at 4°C. Do NOT freeze.
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P-400-5 5 mL $ 154.00
P-400-25 25 mL $ 604.00
P-400-50 50 mL $ 1,113.00
P-400-100 100 mL $ 1,907.00

Protein G HTC Agarose Beads

There is no image for Protein G HTC Agarose Beads Protein G HTC Agarose Beads
Catalog ID Size Pricing
P-430-1 1 mL $ 123.00
P-430-5 5 mL $ 350.00
P-430-25 25 mL $ 1,217.00

Description

Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains of native Protein G have been removed to ensure the maximum specific IgG binding capacity.

GoldBio offers rProtein G resins to isolate and purify classes, subclasses and fragments of immunoglobulins from cell culture media and biological fluids. Rapid purification and high yield of purified immunoglobulin are obtainable by this method.

Protein G is covalently bound to highly cross-linked agarose beads, providing a very stable bond that can greatly minimize leakage of the Protein G and allowing for reuse of the affinity resin in several purification steps. This resin works well in High Flow conditions.

Product Specifications

Catalog ID P-430
Storage/Handling store at 4°C. Do NOT freeze.
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P-430-1 1 mL $ 123.00
P-430-5 5 mL $ 350.00
P-430-25 25 mL $ 1,217.00

Streptavidin Agarose Resin

There is no image for Streptavidin Agarose Resin Streptavidin Agarose Resin
Catalog ID Size Pricing
S-105-1 1 mL $ 59.00
S-105-5 5 mL $ 223.00
S-105-10 10 mL $ 397.00

Description

Streptavidin Agarose Resin is a high affinity biotin-binding chromatography resin with streptavidin covalently bound to 6% crosslinked agarose beads and a biotin binding capacity of >120 nmol/ml of gel. Immobilized Streptavidin resin is suitable for gravity flow columns, spin columns, and FPLC methods. Immobilized Streptavidin can be used for affinity chromatography purifications by separating biotinylated proteins from nonbiotinylated proteins and for affinity purification of antigens when used with biotinylated antibodies.

Product Specifications

Catalog ID S-105
Storage/Handling store at 4°C. Do NOT freeze.
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S-105-1 1 mL $ 59.00
S-105-5 5 mL $ 223.00
S-105-10 10 mL $ 397.00

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