Screening Reagents

In order to observe activity inside certain cells, a screening reagent is used. These agents produce insoluble precipitates of various colors in screening for the presence of certain enzymatic activity. Screening reagents of high quality produce the best results, and using GoldBio branded reagents allows for the clearest results.
Showing 1-20 of 26 items.

2,3,5-Triphenyltetrazolium chloride (TTC; Tetrazolium chloride)

Catalog ID Size Pricing
T-112-25 25 g $ 47.00
T-112-100 100 g $ 158.00
T-112-250 250 g $ 370.00
T-112-500 500 g $ 691.00

Description

Tetrazolium red is used to visualize dehydrogenase enzyme activity. Initially, the tetrazolium solution is colorless but changes to red when it comes into contact with hydrogen. Tetrazolium red is used in a biochemical viability test for seeds. The test relies on dehydrogenase enzymes to release hydrogen ions, which subsequently reduce the colorless tetrazolium salt solution to a red compound called formazan. Living cells turn red while dead cells remain colorless.

Product Specifications

Catalog ID T-112
CAS # 298-96-4
MW 334.81 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C. Protect from light.
View
Quick Add
T-112-25 25 g $ 47.00
T-112-100 100 g $ 158.00
T-112-250 250 g $ 370.00
T-112-500 500 g $ 691.00

4-Methylumbelliferyl caprylate

Catalog ID Size Pricing
M-540-1 1 g $ 121.00
M-540-2.5 2.5 g $ 273.00
M-540-5 5 g $ 530.00
M-540-10 10 g $ 990.00

Description

No description available

Product Specifications

Catalog ID M-540
CAS # 20671-66-3
MW 302.37 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
M-540-1 1 g $ 121.00
M-540-2.5 2.5 g $ 273.00
M-540-5 5 g $ 530.00
M-540-10 10 g $ 990.00

4-Nitrophenyl-α-D- galactopyranoside

Catalog ID Size Pricing
N-280-1 1 g $ 40.00
N-280-2.5 2.5 g $ 77.00
N-280-5 5 g $ 135.00
N-280-10 10 g $ 243.00

Description

No description available

Product Specifications

Catalog ID N-280
CAS # 7493-95-0
MW 301.26 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
N-280-1 1 g $ 40.00
N-280-2.5 2.5 g $ 77.00
N-280-5 5 g $ 135.00
N-280-10 10 g $ 243.00

4-Nitrophenyl-α-D- glucopyranoside (p-Nitrophenyl-α-D-glucoside; PNP-α-D-Glc)

Catalog ID Size Pricing
N-310-2.5 2.5 g $ 50.00
N-310-10 10 g $ 180.00
N-310-25 25 g $ 426.00
N-310-50 50 g $ 810.00

Description

No description available

Product Specifications

Catalog ID N-310
CAS # 3767-28-0
MW 301.26 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
N-310-2.5 2.5 g $ 50.00
N-310-10 10 g $ 180.00
N-310-25 25 g $ 426.00
N-310-50 50 g $ 810.00

4-Nitrophenyl-β-D- galactosidase (p-Nitrophenyl-β-D-galactoside; PNP-β-D-Gal)

Catalog ID Size Pricing
N-290-10 10 g $ 122.00
N-290-25 25 g $ 285.00
N-290-50 50 g $ 535.00
N-290-100 100 g $ 1,000.00

Description

No description available

Product Specifications

Catalog ID N-290
CAS # 3150-24-1
MW 301.26 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
N-290-10 10 g $ 122.00
N-290-25 25 g $ 285.00
N-290-50 50 g $ 535.00
N-290-100 100 g $ 1,000.00

5-Bromo-4-chloro-3-indoxyl butyrate

Catalog ID Size Pricing
B-300-1 1 g $ 255.00
B-300-2.5 2.5 g $ 490.00
B-300-5 5 g $ 754.00
B-300-10 10 g $ 1,160.00

Description

No description available

Product Specifications

Catalog ID B-300
CAS # 129541-43-1
MW 316.59 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
View
Quick Add
B-300-1 1 g $ 255.00
B-300-2.5 2.5 g $ 490.00
B-300-5 5 g $ 754.00
B-300-10 10 g $ 1,160.00

5-Bromo-4-chloro-3-indoxyl caprylate

Catalog ID Size Pricing
B-320-500 500 mg $ 79.00
B-320-2.5 2.5 g $ 221.00
B-320-5 5 g $ 353.00
B-320-10 10 g $ 565.00

Description

No description available

Product Specifications

Catalog ID B-320
CAS # 129541-42-0
MW 372.70 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store under inert gas at -20°C.
View
Quick Add
B-320-500 500 mg $ 79.00
B-320-2.5 2.5 g $ 221.00
B-320-5 5 g $ 353.00
B-320-10 10 g $ 565.00

Magenta-Gal

Catalog ID Size Pricing
B-378-5 5 g $ 219.00
B-378-10 10 g $ 399.00
B-378-25 25 g $ 905.00
B-378-50MG 50 mg $ 23.00

Description

No description available

Product Specifications

Catalog ID B-378
CAS # 93863-88-8
MW 408.63 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
View
Quick Add
B-378-5 5 g $ 219.00
B-378-10 10 g $ 399.00
B-378-25 25 g $ 905.00
B-378-50MG 50 mg $ 23.00

5-Bromo-6-chloro-3-indoxyl butyrate

Catalog ID Size Pricing
B-310-500 500 mg $ 245.00
B-310-1 1 g $ 377.00
B-310-2.5 2.5 g $ 726.00
B-310-5 5 g $ 1,117.00

Description

No description available

Product Specifications

Catalog ID B-310
CAS # 873295-29-5
MW 316.59 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
B-310-500 500 mg $ 245.00
B-310-1 1 g $ 377.00
B-310-2.5 2.5 g $ 726.00
B-310-5 5 g $ 1,117.00

5-Bromo-6-chloro-3-indoxyl caprylate

Catalog ID Size Pricing
B-325-2.5 2.5 g $ 191.00
B-325-5 5 g $ 357.00
B-325-10 10 g $ 667.00
B-325-25 25 g $ 1,558.00

Description

No description available

Product Specifications

Catalog ID B-325
CAS # 209347-94-4
MW 372.70 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C.
View
Quick Add
B-325-2.5 2.5 g $ 191.00
B-325-5 5 g $ 357.00
B-325-10 10 g $ 667.00
B-325-25 25 g $ 1,558.00

Bluo-Gal

Catalog ID Size Pricing
B-673-250 250 mg $ 47.00
B-673-500 500 mg $ 82.00
B-673-1 1 g $ 116.00
B-673-2.5 2.5 g $ 207.00
B-673-10 10 g $ 558.00

Description

Bluo-Gal is used as an alternative to X-Gal, and produces a darker blue color. Bluo-Gal is hydrolyzed by β-galactosidase to produce a blue precipitate. This precipitate is insoluble in alcohol and xylenes, thus making it suitable for immunoblotting and immunocytochemical assays. Bacterial colonies containing active β-galactosidase produce blue colonies when grown on plates containing Bluo-Gal.

Product Specifications

Catalog ID B-673
CAS # 97753-82-7
MW 374.18 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
B-673-250 250 mg $ 47.00
B-673-500 500 mg $ 82.00
B-673-1 1 g $ 116.00
B-673-2.5 2.5 g $ 207.00
B-673-10 10 g $ 558.00

DTNB (Ellman's Reagent)

Catalog ID Size Pricing
D-250-5 5 g $ 73.00
D-250-25 25 g $ 183.00
D-250-50 50 g $ 331.00
D-250-100 100 g $ 388.00

Description

No description available

Product Specifications

Catalog ID D-250
CAS # 69-78-3
MW 396.36 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C. Protect from light and heat.
View
Quick Add
D-250-5 5 g $ 73.00
D-250-25 25 g $ 183.00
D-250-50 50 g $ 331.00
D-250-100 100 g $ 388.00

IPTG

Catalog ID Size Pricing
I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00
I2481C25 25 g $ 115.00
I2481C50 50 g $ 184.00
I2481C100 100 g $ 349.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Description

IPTG is an analog of galactose that is non-metabolizable and inactivates the lac repressor to induce synthesis of β-galactosidase in E. coli. The expression of cloned genes under the control of the lac operon is induced by IPTG. It is also a substrate for thigalactoside transacetylase and has been reported to induce penicillinase in bacteria.

Product Specifications

Catalog ID I2481
CAS # 367-93-1
MW 238.30 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
View
Quick Add
I2481C 1 g $ 17.00
I2481C5 5 g $ 27.00
I2481C25 25 g $ 115.00
I2481C50 50 g $ 184.00
I2481C100 100 g $ 349.00
I2481C200 200 g $ 675.00
I2481C300 300 g $ 899.00
I2481C500 500 g $ 1,407.00
I2481C400 400 g $ 1,168.00

Lapis-3-Acetate

Catalog ID Size Pricing
B-650-1 1 g $ 76.00
B-650-2.5 2.5 g $ 165.00
B-650-5 5 g $ 288.00
B-650-10 10 g $ 500.00

Description

No description available

Product Specifications

Catalog ID B-650
CAS # 17357-14-1
MW 254.09 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
B-650-1 1 g $ 76.00
B-650-2.5 2.5 g $ 165.00
B-650-5 5 g $ 288.00
B-650-10 10 g $ 500.00

MUG

Catalog ID Size Pricing
MUG200 200 mg $ 19.00
MUG1 1 g $ 58.00
MUG5 5 g $ 233.00
MUG10 10 g $ 370.00
MUG25 25 g $ 741.00

Description

4-Methylumbelliferyl beta-D-glucuronide (MUG) is a fluorogenic substrate for beta-glucuronidase (GUS). Upon cleavage, MUG yields blue fluorescence seen under UV light.

This substrate is commonly used for the detection of Escherichia coli through its ability to detect activity of beta-glucuronidase, which is encoded by uidA. GUS activity is tested in minimal medium o-nitrophenyl-beta-D-galactopyranoside (MMO)-MUG.

It is ideal for plant molecular study as a way to examine gene expression under various promoters. And while it works well for measuring activity in plant lysate and leaf disks, it can also work in whole plant assays.

Product Specifications

Catalog ID MUG
CAS # 6160-80-1 or 881005-91-0
MW 388.32 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C.
View
Quick Add
MUG200 200 mg $ 19.00
MUG1 1 g $ 58.00
MUG5 5 g $ 233.00
MUG10 10 g $ 370.00
MUG25 25 g $ 741.00

N-Methylindoxyl-beta-D- galactopyranoside

Catalog ID Size Pricing
M-480-100 100 mg $ 130.00
M-480-500 500 mg $ 525.00
M-480-5 5 g $ 2,283.00

Description

No description available

Product Specifications

Catalog ID M-480
CAS # 207598-26-3
MW 309.32 g/mol
Storage/Handling Store at -20°C. Protect from light.
View
Quick Add
M-480-100 100 mg $ 130.00
M-480-500 500 mg $ 525.00
M-480-5 5 g $ 2,283.00

Nitrotetrazolium blue chloride (NBT)

Catalog ID Size Pricing
NBT1 1 g $ 48.00
NBT2.5 2.5 g $ 98.00
NBT5 5 g $ 158.00
NBT10 10 g $ 254.00

Description

Nitrotetrazolium Blue (NBT) is used in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple-brown final color of the precipitates at the reaction sites.

NBT has also been used with X-Gal,or Salmon-Gal to speed up and enhance colony screening.

Product Specifications

Catalog ID NBT
CAS # 298-83-9
MW 817.65 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store at 4°C. Protect from light.
View
Quick Add
NBT1 1 g $ 48.00
NBT2.5 2.5 g $ 98.00
NBT5 5 g $ 158.00
NBT10 10 g $ 254.00

Salmon Gal (6-Chloro-3-indoxyl-β-D-galactopyranoside)

Catalog ID Size Pricing
C-255-500 500 mg $ 47.00
C-255-1 1 g $ 80.00
C-255-2.5 2.5 g $ 177.00
C-255-5 5 g $ 265.00

Description

Salmon Gal (6-Chloro-3-indolyl-β-D-galactopyranoside) is used with IPTG as an alternative chromogenic substrate capable of detecting LacZ gene encoded ß-galactosidase activity for colony screening in colometric assays.

Unlike colony blue/white screening through X-Gal, nonrecombination results in a salmon color after salmon gal is cleaved by ß-galactosidase through hydrolysis. This contrast may be useful in comparative or secondary screenings.

Incorporating nitroblue tetrazolium salt (NBT) into the salmon gal reactions provides faster results with a clear, purple precipitate than in unaccompanied uses of salmon gal.

Product Specifications

Catalog ID C-255
CAS # 138182-21-5
MW 329.73 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
View
Quick Add
C-255-500 500 mg $ 47.00
C-255-1 1 g $ 80.00
C-255-2.5 2.5 g $ 177.00
C-255-5 5 g $ 265.00

TMB dihydrochloride

Catalog ID Size Pricing
TMBHCL2 2 g $ 68.00
TMBHCL10 10 g $ 299.00
TMBHCL25 25 g $ 576.00
TMBHCL50 50 g $ 885.00

Description

No description available

Product Specifications

Catalog ID TMBHCL
CAS # 207738-08-7
MW 313.27 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at -20°C. Protect from light.
View
Quick Add
TMBHCL2 2 g $ 68.00
TMBHCL10 10 g $ 299.00
TMBHCL25 25 g $ 576.00
TMBHCL50 50 g $ 885.00

TMB, free base

Catalog ID Size Pricing
TMBF10 10 g $ 65.00
TMBF25 25 g $ 153.00
TMBF50 50 g $ 240.00
TMBF100 100 g $ 436.00

Description

No description available

Product Specifications

Catalog ID TMBF
CAS # 54827-17-7
MW 240.35 g/mol
Grade MOLECULAR BIOLOGY GRADE
Storage/Handling Store desiccated at 4°C. Protect from light.
View
Quick Add
TMBF10 10 g $ 65.00
TMBF25 25 g $ 153.00
TMBF50 50 g $ 240.00
TMBF100 100 g $ 436.00

Join our list to receive promos and articles.

Join Now