{"title":"Featured Products","description":"","products":[{"product_id":"simple-stop-1-phosphatase-inhibitor-cocktail","title":"Simple Stop™ 1 Phosphatase Inhibitor Cocktail","description":"\u003cp\u003eWe have reengineered our GoldBio Simple Stop™ 1 to have significantly better protection against Acid Phosphatases as well as enhanced protection against Protein Phosphatase.\u003c\/p\u003e\n\u003cp\u003eSimple Stop™ 1 is a broad-spectrum phosphatase inhibitor cocktail consisting of five phosphatase inhibitors in two solutions that target all the phosphatase categories: serine\/threonine (Ser\/Thr) specific phosphatase, tyrosine specific phosphatase, dual specificity phosphatases, acid phosphatase and alkaline phosphatase.\u003c\/p\u003e\n\u003cp\u003eEach Simple Stop™ phosphatase inhibitor cocktail solution is a 100X concentrated, ready-to-use, solution. Simply add cocktail solution “A” and cocktail solution “B” to your extraction buffers or samples and you are ready to go. This cocktail is ideal for inhibition in tissue extractions and cell lysis experiments and is compatible with most common protein assays.\u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCocktail solution A: 0.25M Sodium Fluoride, 0.1M Sodium Pyrophosphate and 0.05M activated Sodium Orthovanadate\u003c\/li\u003e\n\u003cli\u003eCocktail solution B: 100mM β-glycerophosphate and 1M Sodium Molybdate\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\/Handling:\u003c\/h2\u003e\n\u003cp\u003eStore at 4°C. When stored under the recommended conditions and handled correctly, these products should be stable for at least 2 year from the date of receipt. \u003cbr\u003ePrecipitate may occasionally occur in the solution. To solubilize, heat at 37-48°C for five minute and vortex.\u003c\/p\u003e","brand":"GoldBio","offers":[{"title":"1 mL","offer_id":44854720888889,"sku":"GB-450-1","price":74.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":44854720921657,"sku":"GB-450-5","price":195.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":44854720954425,"sku":"GB-450-10","price":305.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/files\/Default_No_Image_Bottle_png.png?v=1757612830"},{"product_id":"problock-protease-inhibitor-cocktail-100-edta-free","title":"ProBlock™ Protease Inhibitor Cocktail -100, EDTA free","description":"\u003cp\u003eProBlock™-100, EDTA Free is designed for large volumes of extraction\/lysis buffers and is supplied as 10 vials per kit, with each vial  suitable for 100 ml.  \u003cbr\u003e\u003cbr\u003e ProBlock-100, EDTA Free protease inhibitor cocktail contains optimized  concentrations of various protease inhibitors, which provide excellent  inhibition of protease activities during protein purification. ProBlock™   is a superior general protease inhibitor cocktail that is suitable for  purification from mammalian, plant, bacteria and yeast samples.  The  cocktail is EDTA free and contains both irreversible and reversible  protease inhibitors to inhibit serine, cysteine and other proteases. At  1X concentration, ProBlock™  inhibits over 90% of protease activities  (e.g. Mouse Pancreas Extract 0.5 mg\/ml protein). \u003c\/p\u003e \u003cp\u003eStorage\/Handling: Store at 4°C. When stored under the recommended conditions and handled correctly, these products should be stable for at least 2 year from the date of receipt. \u003cbr\u003e \u003c\/p\u003e","brand":"GoldBio","offers":[{"title":"10 Vials","offer_id":44854721740857,"sku":"GB-328-100","price":604.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/files\/GB-328-100.jpg?v=1757446934"},{"product_id":"problock-gold-extra-strength-protease-inhibitor-cocktail","title":"ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail","description":"\u003cp class=\"MsoNormal\"\u003eGoldBio's ProBlock™ Gold Extra Strength Protease Inhibitor Cocktail is a concentrated, broad-spectrum inhibitor formulation designed for demanding protein extraction and purification applications where increased protease protection is needed.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eIt contains 50% higher levels of protease inhibitors than the \u003ca href=\"https:\/\/www.goldbio.com\/products\/problock-gold-protease-inhibitor-cocktail-100x\"\u003estandard ProBlock™ Gold\u003c\/a\u003e formulation and combines reversible and irreversible inhibitors targeting serine, cysteine, aspartic, aminopeptidase, and other protease activity.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eAt a 1X concentration in extraction buffer at pH 7 to 8, ProBlock™ Gold Extra Strength inhibits more than 90% of protease activity in GoldBio's mouse pancreas extract test system.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eThe disclosed formulation contains AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF. Combining inhibitors with different target specificities provides broader protection than relying on a single protease inhibitor.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eThe Extra Strength formulation is especially useful for samples with high endogenous protease activity or when maximizing recovery of intact proteins during initial extraction and purification is important.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eProBlock™ Gold Extra Strength is supplied with separate EDTA and 1,10-phenanthroline monohydrate solutions for optional metalloprotease inhibition. Researchers can add either chelator when additional metalloprotease protection is needed or leave them out when metal chelation could interfere with the experiment.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eThis is particularly important for proteins that require Ca²⁺, Mg²⁺, or Mn²⁺ for biological activity and for immobilized metal affinity chromatography. Omitting EDTA and 1,10-phenanthroline can also improve compatibility with applications such as isoelectric focusing.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003cul style=\"margin-top: 0in;\" type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo1; tab-stops: list .5in;\"\u003eProBlock™ Gold Extra Strength Protease Inhibitor Cocktail\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo1; tab-stops: list .5in;\"\u003eEDTA (0.5 M Solution, pH 8.0)\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo1; tab-stops: list .5in;\"\u003e1,10-Phenanthroline monohydrate (1 M Solution [100X])\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eCocktail Protease Inhibitors:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003eAEBSF\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eAprotinin\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eBestatin\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eE-64\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eLeupeptin\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003ePepstatin A\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003ePMSF\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eMechanism\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003eProBlock™ Gold Extra Strength uses a multi-inhibitor approach to provide increased protection against proteolysis in samples with elevated or difficult-to-control protease activity. Its higher inhibitor content provides increased inhibitor availability during protein extraction and purification from samples with substantial protease activity. The combination targets multiple protease classes simultaneously, reducing the likelihood that active endogenous proteases will continue degrading proteins after cells or tissues are disrupted.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eComparison\u003c\/h2\u003e\n\u003ctable class=\"MsoNormalTable\" border=\"0\" cellpadding=\"6\" cellspacing=\"0\" style=\"width: 100%; max-width: 100%; border-collapse: collapse; table-layout: fixed;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eFeature\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eProBlock™ Gold Extra Strength\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e(Catalog Number: GB-116)\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eProBlock™ Gold [100X]\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e(Catalog Number: GB-108)\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eBest For\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eMore demanding protein extraction and purification where increased inhibitor strength is desired\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eRoutine protein extraction and purification\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eCore Inhibitors\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eAEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eAEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eProtease Coverage\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eBroad-spectrum inhibition with an enhanced dose of protease inhibitors\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eBroad-spectrum inhibition of serine, cysteine, and other proteases\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eMetalloprotease Inhibition\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eSeparate EDTA and 1,10-phenanthroline monohydrate solutions included\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eSeparate EDTA solution included\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eEDTA\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eOptional\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eOptional\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e1,10-Phenanthroline\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eIncluded separately for additional metalloprotease inhibition\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eNot included\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eMetal-Sensitive Applications\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eEDTA and 1,10-phenanthroline can be omitted when divalent cations must be preserved\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eEDTA can be omitted when divalent cations must be preserved\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eStorage\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e4°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003e4°C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 22%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eStability\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eAt least 1 year when stored and handled as recommended\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 39%; padding: 6px;\"\u003e\n\u003cp class=\"MsoNormal\"\u003eAt least 1 year when stored and handled as recommended\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eCommon Applications\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003e(Click each for more information)\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eProtein Purification from Protease-Rich Samples \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To provide increased protection against protein degradation when routine protease inhibition may not provide sufficient coverage.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: The Extra Strength formulation contains 50% more protease inhibitors than standard ProBlock™ Gold. Its combination of AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF targets multiple protease classes simultaneously.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Protein purification from protease-rich samples, difficult tissue extracts, purification of proteolytically sensitive proteins, and experiments where maximizing recovery of intact protein is important.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003ePreparation of Lysates for Quantitative Protein Assays \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To preserve proteins during tissue extraction before quantitative analysis of specific protein targets.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: Adding the inhibitor cocktail during homogenization limits proteolytic changes that could alter the amount or integrity of proteins subsequently measured. This is particularly useful when samples will be stored before analysis.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: ELISA, cytokine quantification, comparative protein analysis, and preparation of frozen lysates for later measurement.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eRomero, J., Toral-Rios, D., Yu, J., Paul, S. M., \u0026amp; Cashikar, A. G. (2024). 25-hydroxycholesterol promotes brain cytokine production and leukocyte infiltration in a mouse model of lipopolysaccharide-induced neuroinflammation. \u003cem\u003eJournal of Neuroinflammation\u003c\/em\u003e, 21, 251. https:\/\/doi.org\/10.1186\/s12974-024-03233-1\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eBenefits:\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003eGreater Protease Inhibition: Provides \u0026gt;90% protease inhibition at 1X concentration and pH 7–8 in GoldBio's mouse pancreas extract test system.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eContains 50% higher levels of protease inhibitors than standard ProBlock™ Gold.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eSupports Recovery of Intact Proteins: Increased inhibitor levels help reduce proteolytic degradation during initial extraction and purification, supporting recovery of intact target proteins from protease-rich samples.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eDisclosed Formulation: Contains AEBSF, aprotinin, bestatin, E-64, leupeptin, pepstatin A, and PMSF.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eMultiple package sizes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eStorage\/Handling:\u003c\/h2\u003e\n\u003cp\u003eStore at 4°C. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"GoldBio","offers":[{"title":"2 mL","offer_id":44854722199609,"sku":"GB-116-2","price":185.0,"currency_code":"USD","in_stock":true},{"title":"5 mL","offer_id":44854722232377,"sku":"GB-116-5","price":364.0,"currency_code":"USD","in_stock":true},{"title":"10 mL","offer_id":44854722265145,"sku":"GB-116-10","price":637.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/files\/GB-116.jpg?v=1783547386"},{"product_id":"problock-gold-2d-protease-inhibitor-cocktail-edta-free-100x","title":"ProBlock™ Gold 2D Protease Inhibitor Cocktail (EDTA Free) [100X]","description":"\u003cp\u003eProBlock™ Gold 2D    is a protease inhibitor cocktail specifically developed for sample preparation for IEF\/2D- studies. No EDTA is used, which allows optimal action of nuclease activity for removing nucleic acids from the samples.\u003cbr\u003e\u003cbr\u003e ProBlock™ Gold 2D contains both irreversible and reversible protease inhibitors and inhibits serine, cysteine and metalloproteases etc. Due to the optimized concentration of the various inhibitors, the ProBlock™ Gold 2D   shows excellent inhibition of protease activities and is therefore suitable for the protection of proteins extracted from animal cells \/ tissues, plants, yeast and bacteria etc.  \u003cbr\u003e\u003cbr\u003e ProBlock™ Gold 2D at 1X concentration in extraction buffer at pH 7-8 inhibits over 90% of protease activities. \u003c\/p\u003e \u003cp\u003eStorage\/Handling: Store at -20°C. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt. \u003cbr\u003e \u003c\/p\u003e","brand":"GoldBio","offers":[{"title":"1 mL","offer_id":44854722297913,"sku":"GB-109-1","price":228.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/files\/4495-GB-109-1.jpg?v=1757446946"}],"url":"https:\/\/www.goldbio.com\/collections\/featured-products.oembed","provider":"GoldBio","version":"1.0","type":"link"}