{"title":"Monthly Highlights","description":"\u003cp data-start=\"57\" data-end=\"394\"\u003eFrom cell lysis to protein purification, the quality of your results depends on protecting proteins from degradation at every step. This month, we're highlighting GoldBio's protease inhibitors, ProBlock™ protease inhibitor cocktails, and lysis buffers designed to help you maximize protein recovery and preserve your sample's integrity.\u003c\/p\u003e\n\u003cp data-start=\"396\" data-end=\"856\" data-is-last-node=\"\" data-is-only-node=\"\"\u003eWhether you're using AEBSF or aprotinin for targeted protease inhibition, ProBlock™ cocktails for broad-spectrum protection, or Dr. Gold's Bacterial Protein Extraction Reagent for rapid protein extraction, GoldBio offers dependable solutions that save time, simplify preparation, and support consistent results, all for the affordable prices you've come to expect.\u003c\/p\u003e","products":[{"product_id":"dr-golds-bacterial-protein-extraction-reagent","title":"Dr. Gold's Bacterial Protein Extraction Reagent","description":"\u003ch3 style=\"text-align: center;\"\u003e  Enhanced Performance | Greater Yields | Cost-Effective\u003cstrong\u003e\u003c\/strong\u003e\n\u003c\/h3\u003e \u003cp\u003e\u003cbr\u003e\u003cstrong\u003eDr. Gold’s Bacterial Protein Extraction Reagent\u003c\/strong\u003e is a powerful, next-generation solution designed for efficient extraction of expressed proteins from bacterial cells—now reengineered for improved performance and cost-effectiveness. Built on the foundation of our trusted Bacterial Cell Lysis Buffer, this ready-to-use formulation offers enhanced yield and reproducibility without the need for harsh mechanical disruption. \u003c\/p\u003e \u003cp\u003eOur \u003cstrong\u003eproprietary blend \u003c\/strong\u003eof buffering agents and dialyzable, non-denaturing detergents \u003cstrong\u003eensures gentle but thorough lysis\u003c\/strong\u003e, preserving native protein structure and activity. \u003c\/p\u003e \u003cp\u003eThe reagent includes lysozyme, nucleases, and a specially optimized salt solution to promote full membrane disruption and maximize the recovery of soluble proteins. \u003c\/p\u003e \u003cp\u003e\u003cbr\u003e\u003cstrong\u003e\u003cem\u003eWhether you're preparing samples for SDS-PAGE, purification, or enzymatic assays, Dr. Gold’s reagent offers superior protein extraction with consistent, repeatable results—batch after batch.\u003cbr\u003e\u003c\/em\u003e\u003c\/strong\u003e \u003c\/p\u003e \u003cp\u003e\u003cstrong\u003e\u003cem\u003e\u003cbr\u003e\u003c\/em\u003e\u003c\/strong\u003e \u003c\/p\u003e \u003ch2\u003eKey Features \u003c\/h2\u003e \u003cul\u003e \u003cli\u003e\n\u003cstrong\u003eReady-to-use formulation\u003c\/strong\u003e—no additional components required \u003c\/li\u003e \u003cli\u003eContains both non-ionic and zwitterionic detergents in Tris buffer \u003c\/li\u003e \u003cli\u003eIncludes lysozyme and nucleases for complete cell breakdown \u003c\/li\u003e \u003cli\u003eEnhanced salt formulation improves soluble protein recovery \u003c\/li\u003e \u003cli\u003eCompatible with common reducing agents and protease inhibitors \u003c\/li\u003e \u003cli\u003eSuitable for use with both fresh and frozen bacterial pellets \u003c\/li\u003e \u003cli\u003e\n\u003cstrong\u003eOutperforms leading competitors\u003c\/strong\u003e in soluble protein yield and clarity \u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eKit Component\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eDr. Gold’s Bacterial Protein Extraction Reagent  \u003c\/li\u003e \u003cli\u003eGB Enzyme Mix  \u003c\/li\u003e \u003cli\u003eSalt Solution  \u003c\/li\u003e \u003c\/ul\u003e \u003cblockquote\u003eNote: The Salt Solution is not strictly required for lysis, but our research has shown it significantly enhances protein solubility and yield. \u003c\/blockquote\u003e \u003ch2\u003eStorage\/Handling\u003c\/h2\u003e \u003cp\u003eThis product may be shipped with blue ice. Dr. Gold’s Bacterial Protein Extraction Reagent and Salt Solution should be stored at 4°C, GB Enzyme Mix should be stored at -20°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt. \u003c\/p\u003e \u003cp\u003e\u003cbr\u003e \u003c\/p\u003e \u003ch2\u003eFAQ \u003c\/h2\u003e \u003ch3\u003eHow much reagent do I need to use with my culture? \u003c\/h3\u003e \u003cp\u003eUse 1.3 mL of extraction buffer per 10 mL of culture at A\u003csub\u003e600\u003c\/sub\u003e = 2.0. Adjust proportionally for different cell densities and volumes. \u003c\/p\u003e \u003cp\u003e\u003cbr\u003e \u003c\/p\u003e \u003ch3\u003eDoes the reagent contain EDTA or other chelating agents? is the reagent compatible with metal affinity chromatography (His tagged proteins)?\u003cbr\u003e\n\u003c\/h3\u003e \u003cp\u003eNo! Dr. Gold’s Bacterial Protein Extraction Reagent does not contain EDTA or other chelating agents. It is fully compatible with His-Tag affinity chromatography.\u003cbr\u003e \u003c\/p\u003e \u003cp\u003e\u003cbr\u003e \u003c\/p\u003e \u003ch3\u003eCan proteins isolated using Dr. Gold’s Bacterial Protein Extraction Reagent be quantified using Bradford or BCA or will there be background due to lysozyme and nucleases?\u003cbr\u003e\n\u003c\/h3\u003e \u003cp\u003eThere may be some background signal from the added enzymes, but in our testing it's not enough to skew the total extraction results (Levels are undetectable on SDS-Page Gels of extracted material)\u003cspan class=\"redactor-invisible-space\"\u003e.\u003cbr\u003e\u003c\/span\u003e \u003c\/p\u003e \u003cp\u003e\u003cbr\u003e \u003c\/p\u003e \u003cp\u003e\u003cbr\u003e\u003cspan class=\"redactor-invisible-space\"\u003e\u003c\/span\u003e \u003c\/p\u003e","brand":"GoldBio","offers":[{"title":"25 mL","offer_id":44854720561209,"sku":"GB-500-25","price":22.0,"currency_code":"USD","in_stock":true},{"title":"50 mL","offer_id":44854720593977,"sku":"GB-500-50","price":36.0,"currency_code":"USD","in_stock":true},{"title":"100 mL","offer_id":44854720626745,"sku":"GB-500-100","price":64.0,"currency_code":"USD","in_stock":true},{"title":"500 mL","offer_id":44854720659513,"sku":"GB-500-500","price":122.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/files\/Gb-500_-_Dr._Golds_Protein_Extraction_Reagent.jpg?v=1760382796"},{"product_id":"aebsf","title":"AEBSF","description":"\u003cp\u003e AEBSF is an irreversible serine protease inhibitor. AEBSF has been shown  to inhibit trypsin, chymotrypsin, plasmin, kallikrein and thrombin. It  inhibits the active site of the enzyme through acylation. As an  alternative to PMSF and DFP, AEBSF offers lower toxicity, improved  solubility in water and improved stability in aqueous solutions. AEBSF  has been used in cell culture in concentrations of up to 0.25mM.  Aqueous solutions are stable between pH 5-6; limited stability above pH  7.5. \u003c\/p\u003e \u003cblockquote\u003e\n\u003cstrong\u003e Attention Canadian and Mexican customers: This product is a hazardous and can only ship via Air. \u003c\/strong\u003e \u003c\/blockquote\u003e \u003ch2\u003eApplications\u003c\/h2\u003e \u003cul\u003e \u003cli\u003eSerine Protease inhibitor\u003c\/li\u003e \u003cli\u003e\n\u003cspan class=\"ILfuVd\"\u003e\u003cspan class=\"hgKElc\"\u003eSREBP\u003c\/span\u003e\u003c\/span\u003e protein activation\u003c\/li\u003e \u003cli\u003ePMSF\/DFP alternative\u003c\/li\u003e \u003c\/ul\u003e \u003ch2\u003eStorage and Handling\u003c\/h2\u003e \u003cp\u003eStore at -20°C. Protect from light. \u003c\/p\u003e \u003ch2\u003eWorking Concentration\u003c\/h2\u003e \u003cp\u003eMake stock solutions at 100mM. Aliquot and store at -20°C for up to 2 months. \u003cbr\u003eAEBSF working concentrations are usually between 0.1-1.0mM and should be made daily.\u003cbr\u003e\u003cbr\u003e \u003c\/p\u003e","brand":"GoldBio","offers":[{"title":"500 mg","offer_id":44854847995961,"sku":"A-540-500","price":59.0,"currency_code":"USD","in_stock":true},{"title":"1 g","offer_id":44854848028729,"sku":"A-540-1","price":104.0,"currency_code":"USD","in_stock":true},{"title":"2.5 g","offer_id":44854848061497,"sku":"A-540-2.5","price":224.0,"currency_code":"USD","in_stock":true},{"title":"5 g","offer_id":44854848094265,"sku":"A-540-5","price":342.0,"currency_code":"USD","in_stock":true},{"title":"10 g","offer_id":44854848127033,"sku":"A-540-10","price":624.0,"currency_code":"USD","in_stock":true},{"title":"25 g","offer_id":44854848159801,"sku":"A-540-25","price":1415.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/files\/A-540.jpg?v=1757448005"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/collections\/Gb-500_-_Dr._Golds_Protein_Extraction_Reagent.jpg?v=1780685493","url":"https:\/\/www.goldbio.com\/collections\/monthly-highlights.oembed","provider":"GoldBio","version":"1.0","type":"link"}