{"title":"New Competent Cells","description":"\u003cp\u003eGoldBio is always expanding our competent cell portfolio to give you more options. Discover some of our newest additions, offering the quality, value, and selection you've come to expect. Explore each product to find detailed specifications.\u003c\/p\u003e","products":[{"product_id":"bl21-de3-plyss-chemically-competent-e-coli-cells","title":"BL21 (DE3) pLysS Chemically Competent E. coli Cells","description":"\u003cp class=\"MsoNormal\"\u003eGoldBio’s BL21(DE3) pLysS Chemically Competent \u003ci\u003eE. coli\u003c\/i\u003e Cells provide tight control of T7 promoter-driven gene expression. This makes them ideal for expressing toxic or unstable recombinant proteins. \u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eThese cells carry a chromosomal T7 RNA polymerase gene under IPTG-inducible control. They also contain the pLysS plasmid that expresses T7 lysozyme. This suppresses basal expression and allows for precise regulation before induction.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eThey're compatible with standard heat-shock protocols, making them a reliable host for protein expression, cloning, and co-expression studies.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003chr\u003e\n\u003cp\u003eBL21 (DE3) pLysS Chemically Competent E. coli Cells are \u003cstrong\u003efree of animal-derived products\u003c\/strong\u003e and grown with animal-free media.\u003c\/p\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul style=\"margin-top: 0in;\" type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo2; tab-stops: list .5in;\"\u003eCompetent Cells\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo2; tab-stops: list .5in;\"\u003e1 x 12 mL Recovery Media\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo2; tab-stops: list .5in;\"\u003e1 x 10 µL Control Plasmid (\u003ca href=\"https:\/\/goldbio.com\/product\/puc19-plasmid\"\u003epUC19 Control\u003c\/a\u003e, 500 pg\/µL)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eReagents Needed for One Reaction\u003c\/h2\u003e\n\u003cul style=\"margin-top: 0in;\" type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l1 level1 lfo3; tab-stops: list .5in;\"\u003eBL21 (DE3) pLysS chemically competent cells: 50 µL\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l1 level1 lfo3; tab-stops: list .5in;\"\u003eDNA (or \u003ca href=\"https:\/\/goldbio.com\/product\/puc19-plasmid\"\u003epUC19 Control\u003c\/a\u003e, 500 pg\/µL): 2 µL\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l1 level1 lfo3; tab-stops: list .5in;\"\u003eRecovery medium: 1 mL\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\/Handling\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003eThis product may be shipped on dry ice. BL21 (DE3) pLysS Chemically Competent \u003ci\u003eE. coli\u003c\/i\u003e cells should be stored at -80°C, pUC19 Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.\u003c\/p\u003e\n\u003ch2\u003eGenomic Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli class=\"MsoNormal\"\u003e\n\u003cspan style=\"mso-spacerun: yes;\"\u003e \u003c\/span\u003e≥1 × 10\u003csup\u003e6\u003c\/sup\u003e cfu\/µg efficiency\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eWidely used host background\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eT7 expression strain\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eContains pLysS plasmid expressing T7 lysozyme to reduce basal (leaky) expression\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eChloramphenicol resistant (for pLysS plasmid maintenance)\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eDeficient in both lon and ompT proteases\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eResistant to phage T1 (\u003ci\u003efhuA2\u003c\/i\u003e)\u003c\/li\u003e\n\u003cli class=\"MsoNormal\"\u003eB strain\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eGenotype\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003eF\u003cspan style=\"font-family: 'Cambria Math',serif; mso-bidi-font-family: 'Cambria Math';\"\u003e⁻\u003c\/span\u003e ompT hsdS\u003csub\u003eB\u003c\/sub\u003e(r\u003csub\u003eB\u003c\/sub\u003e\u003cspan style=\"font-family: 'Cambria Math',serif; mso-bidi-font-family: 'Cambria Math';\"\u003e⁻\u003c\/span\u003e m\u003csub\u003eB\u003c\/sub\u003e\u003cspan style=\"font-family: 'Cambria Math',serif; mso-bidi-font-family: 'Cambria Math';\"\u003e⁻\u003c\/span\u003e) gal dcm (DE3) pLysS (Cam\u003csup\u003eR\u003c\/sup\u003e)\u003c\/p\u003e\n\u003ch2\u003eFunctional Highlights and Mechanism\u003c\/h2\u003e\n\u003cp class=\"MsoListParagraph\" style=\"text-indent: -.25in; mso-list: l2 level1 lfo1;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-family: Symbol; mso-fareast-font-family: Symbol; mso-bidi-font-family: Symbol;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e·\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e         \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003eControlled T7 Expression System\u003c\/b\u003e: BL21(DE3)pLysS cells carry a chromosomal DE3 lysogen encoding T7 RNA polymerase under lacUV5 control. This system enables IPTG-inducible transcription of genes cloned downstream of T7 promoters.\u003c\/p\u003e\n\u003cp class=\"MsoListParagraph\" style=\"text-indent: -.25in; mso-list: l2 level1 lfo1;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-family: Symbol; mso-fareast-font-family: Symbol; mso-bidi-font-family: Symbol;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e·\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e         \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003epLysS-Mediated Basal Suppression:\u003c\/b\u003e The pLysS plasmid constitutively expresses low levels of T7 lysozyme, which binds to and inhibits T7 RNA polymerase. This inhibits transcriptional activity in the absence of induction, reducing leaky expression that can be toxic to the host.\u003c\/p\u003e\n\u003cp class=\"MsoListParagraph\" style=\"text-indent: -.25in; mso-list: l2 level1 lfo1;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-family: Symbol; mso-fareast-font-family: Symbol; mso-bidi-font-family: Symbol;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e·\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e         \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003eHigh Transformation Efficiency\u003c\/b\u003e: Chemically competent preparation ensures reliable uptake of plasmid DNA, including large or low-copy T7 vectors, using standard heat-shock protocols.\u003c\/p\u003e\n\u003cp class=\"MsoListParagraphCxSpFirst\" style=\"text-indent: -.25in; mso-list: l2 level1 lfo1;\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan style=\"font-family: Symbol; mso-fareast-font-family: Symbol; mso-bidi-font-family: Symbol;\"\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e·\u003cspan style=\"font: 7.0pt 'Times New Roman';\"\u003e         \u003c\/span\u003e\u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cb\u003eProtease and Endonuclease Deficiency:\u003c\/b\u003e The ompT and hsdSB mutations reduce degradation of heterologous proteins and plasmid DNA, respectively, preserving protein integrity and plasmid stability during expression and cloning.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Applications and Usage Notes\u003c\/h2\u003e\n\u003ch3\u003eRecommended Applications\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eExpression of Toxic or Unstable Recombinant Proteins\u003c\/b\u003e\u003cbr\u003eIdeal for proteins that impair host viability when expressed under leaky systems.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eInducible Expression of Tagged Proteins\u003c\/b\u003e\u003cbr\u003eSuitable for 6xHis, GST, or MBP fusion proteins in IPTG-inducible T7 systems.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003ePlasmid Screening and Optimization Studies\u003c\/b\u003e\u003cbr\u003eUseful in testing different expression constructs for yield, solubility, and activity.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eDual Expression or Co-expression of Multi-Subunit Proteins\u003c\/b\u003e\u003cbr\u003eCompatible with dual-plasmid setups when using vectors with compatible origins and resistance markers.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eSuppressed Background Expression for Library Screening\u003c\/b\u003e\u003cbr\u003eReduces false positives caused by uninduced expression in screening experiments.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch3\u003eUsage Tips and Considerations\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eMaintain Chloramphenicol Selection\u003c\/b\u003e\u003cbr\u003eAlways grow cells in medium containing chloramphenicol (25 µg\/ml) to retain the pLysS plasmid.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eAvoid Overgrowth Before Induction\u003c\/b\u003e\u003cbr\u003eInduce at mid-log phase (OD600 ~0.4–0.6) to maximize expression and minimize stress on the host.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eLower Expression Temperature for Soluble Proteins\u003c\/b\u003e\u003cbr\u003eConsider inducing at 16–25°C to improve solubility and reduce aggregation of challenging proteins.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eUse Freshly Prepared IPTG\u003c\/b\u003e\u003cbr\u003eEnsure IPTG is fresh and stored properly to maintain consistent induction across experiments.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eAliquot Cells Upon Receipt\u003c\/b\u003e\u003cbr\u003eMinimize freeze-thaw cycles by aliquoting single-use portions and storing at –80°C.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003eTest Expression Gradually\u003c\/b\u003e\u003cbr\u003eStart with small-scale expressions (2–10 ml) to determine optimal IPTG concentration (commonly 0.1–1 mM).\u003c\/p\u003e\n\u003ch2\u003eCommon Research Applications\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003e\u003cstrong\u003e(Click each for more information)\u003c\/strong\u003e\u003c\/em\u003e\u003c\/p\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eTightly Regulated Expression of Toxic Recombinant Proteins \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To reduce leaky expression of proteins that are toxic to \u003cem\u003eE. coli\u003c\/em\u003e prior to induction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: The pLysS plasmid expresses T7 lysozyme, which binds to and inhibits T7 RNA polymerase. This suppresses basal transcription from the T7 promoter until induction with IPTG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Expression of antimicrobial peptides, proteases, membrane proteins, or other unstable products.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eStudier, F. W. (1991). Use of bacteriophage T7 lysozyme to improve an inducible T7 expression system. \u003cem\u003eJournal of Molecular Biology\u003c\/em\u003e, 219(1), 37–44. https:\/\/doi.org\/10.1016\/0022-2836(91)90855-Z\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eHigh-Level Recombinant Protein Expression Using T7 Promoter Systems \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To produce large amounts of recombinant protein under IPTG induction.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: BL21(DE3)pLysS contains a chromosomal copy of the T7 RNA polymerase gene under lacUV5 control. Upon IPTG addition, T7 polymerase is produced and drives transcription of T7 promoter-controlled genes on the expression plasmid.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Protein purification, enzyme assays, structural biology.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eStudier, F. W., \u0026amp; Moffatt, B. A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. \u003cem\u003eJournal of Molecular Biology\u003c\/em\u003e, 189(1), 113–130. https:\/\/doi.org\/10.1016\/0022-2836(86)90385-2\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eMinimized Background Expression in Protein Optimization Workflows \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To optimize expression conditions (e.g., temperature, induction time, plasmid design) for improved protein yield and solubility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: The presence of pLysS improves experiment reproducibility by reducing unintended basal expression of the target gene, allowing for cleaner optimization.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Protein solubility screening, mutant library expression, tagged protein system trials.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eSørensen, H. P., \u0026amp; Mortensen, K. K. (2005). Soluble expression of recombinant proteins in the cytoplasm of \u003cem\u003eEscherichia coli\u003c\/em\u003e. \u003cem\u003eMicrobial Cell Factories\u003c\/em\u003e, 4, 1. https:\/\/doi.org\/10.1186\/1475-2859-4-1\u003cbr\u003eGrossman, T. H., Kawasaki, E. S., Punreddy, S. R., \u0026amp; Osburne, M. S. (1998). Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. \u003cem\u003eGene\u003c\/em\u003e, 209(1–2), 95–103.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003e\u003cstrong\u003eEfficient Cloning and Transformation of T7-Based Expression Plasmids \u003cbr\u003e\u003c\/strong\u003e\u003c\/summary\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurpose\u003c\/strong\u003e: To serve as a host strain for transformation and propagation of T7 expression vectors.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHow It Works\u003c\/strong\u003e: These cells are chemically competent, allowing for rapid uptake of plasmid DNA, including high-copy or inducible expression plasmids.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications\u003c\/strong\u003e: Plasmid construction, site-directed mutagenesis, library transformation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/details\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003eTransformation efficiency is tested by using the pUC19 control DNA supplied with the kit and using the protocol given below. Transformation efficiency should be ≥4 x 10\u003csup\u003e7\u003c\/sup\u003e CFU\/µg pUC19 DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eGeneral Guidelines\u003c\/p\u003e\n\u003cul style=\"margin-top: 0in;\" type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo1; tab-stops: list .5in;\"\u003eHandle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting.\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l0 level1 lfo1; tab-stops: list .5in;\"\u003eThaw competent cells on ice and transform cells immediately following thawing. After adding DNA, mix by tapping the tube gently. Do not mix cells by pipetting or vortexing.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e\n\u003ch2\u003eCalculation of Transformation Efficiency\u003c\/h2\u003e\n\u003cp class=\"MsoNormal\"\u003eTransformation Efficiency (TE) is defined as the number of colony forming units (cfu) produced by transforming 1 µg of plasmid into a given volume of competent cells.\u003c\/p\u003e\n\u003cul style=\"margin-top: 0in;\" type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l1 level1 lfo2; tab-stops: list .5in;\"\u003eTE = Colonies\/µg\/Dilution\u003c\/li\u003e\n\u003cul style=\"margin-top: 0in;\" type=\"disc\"\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l1 level2 lfo2; tab-stops: list 1.0in;\"\u003eColonies = the number of colonies counted\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l1 level2 lfo2; tab-stops: list 1.0in;\"\u003eµg = amount of DNA transformed in µg\u003c\/li\u003e\n\u003cli class=\"MsoNormal\" style=\"mso-list: l1 level2 lfo2; tab-stops: list 1.0in;\"\u003eDilution = total dilution of the DNA before plating\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/ul\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003ci\u003eExample\u003c\/i\u003e\u003c\/b\u003e\u003ci\u003e: Transform 1 µl of (10 pg\/µl) control plasmid into 25 µl of cells, add 975 µl of Recovery Medium. Dilute 10 µl of this in 990 µl of Recovery Medium and plate 50 µl. Count the colonies on the plate the next day. If you count 250 colonies, the TE is calculated as follows:\u003cbr\u003e\u003cbr\u003eColonies = 250\u003cbr\u003eµg of DNA = 0.00001\u003cbr\u003eDilution = 10\/1000 x 50\/1000 = 0.0005\u003cbr\u003eTE = 250\/0.00001\/0.0005 = 5.0 × 10\u003csup\u003e10\u003c\/sup\u003e\u003c\/i\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/goldbio.com\/product\/puc19-plasmid\" target=\"_blank\"\u003e\u003cimg style=\"width: 737px; height: 240px;\" alt=\"pUC Plasmid Vector\" height=\"240\" width=\"737\" src=\"https:\/\/commercio.nyc3.digitaloceanspaces.com\/goldbio-2018\/pages\/pUC%20Plasmid%20Card.PNG\"\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"GoldBio","offers":[{"title":"5 x 50 μL","offer_id":45264546103353,"sku":"CC-123-5x50","price":61.0,"currency_code":"USD","in_stock":true},{"title":"10 x 50 μL","offer_id":45264546136121,"sku":"CC-123-10x50","price":109.0,"currency_code":"USD","in_stock":true},{"title":"15 x 50 μL","offer_id":45264546168889,"sku":"CC-123-15x50","price":144.0,"currency_code":"USD","in_stock":true},{"title":"20 x 50 μL","offer_id":45264546201657,"sku":"CC-123-20x50","price":170.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/files\/CC-123-BL21_DE3_pLysSChemicallyCompetentE.coliCells.jpg?v=1766165731"},{"product_id":"fold-assist-chemically-competent-e-coli-cell-bundle","title":"Fold-Assist Chemically Competent E. Coli Cell Bundle","description":"\u003ch3 data-section-id=\"zacffh\" data-start=\"253\" data-end=\"310\"\u003eFold-Assist Chemically Competent E. Coli Cell Bundle\u003c\/h3\u003e\n\u003cp data-end=\"474\" data-start=\"202\"\u003eDesigned to support the expression of challenging recombinant proteins, the Fold-Assist™ Chaperone Competent Cell Bundle features \u003cem data-start=\"1302\" data-end=\"1311\"\u003eE. coli\u003c\/em\u003e BL21 (DE3) strains engineered to promote proper folding through co-expression of specialized molecular chaperone systems.\u003c\/p\u003e\n\u003cp data-end=\"803\" data-start=\"476\"\u003eThis bundle includes five strains, each carrying a distinct Fold-Assist™ chaperone plasmid (pFA series), designed to provide complementary folding environments for difficult targets. These systems correspond to widely used chaperone configurations and include combinations of DnaK\/DnaJ\/GrpE (KJE), GroEL\/ES, and Trigger Factor:\u003c\/p\u003e\n\u003cul data-end=\"1034\" data-start=\"805\"\u003e\n\u003cli data-end=\"868\" data-start=\"805\" data-section-id=\"1yrk1qt\"\u003e\n\u003cp data-end=\"868\" data-start=\"807\"\u003e\u003cstrong data-end=\"822\" data-start=\"807\"\u003epFA-KJE-Gro\u003c\/strong\u003e — combined DnaK\/DnaJ\/GrpE + GroEL\/ES system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"908\" data-start=\"869\" data-section-id=\"19gt6x2\"\u003e\n\u003cp data-end=\"908\" data-start=\"871\"\u003e\u003cstrong data-end=\"882\" data-start=\"871\"\u003epFA-KJE\u003c\/strong\u003e — DnaK\/DnaJ\/GrpE system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"942\" data-start=\"909\" data-section-id=\"g3gmtv\"\u003e\n\u003cp data-end=\"942\" data-start=\"911\"\u003e\u003cstrong data-end=\"922\" data-start=\"911\"\u003epFA-Gro\u003c\/strong\u003e — GroEL\/ES system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"995\" data-start=\"943\" data-section-id=\"uhlxpb\"\u003e\n\u003cp data-end=\"995\" data-start=\"945\"\u003e\u003cstrong data-end=\"958\" data-start=\"945\"\u003epFA-GroTF\u003c\/strong\u003e — GroEL\/ES + Trigger Factor system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"1034\" data-start=\"996\" data-section-id=\"7mgcv8\"\u003e\n\u003cp data-end=\"1034\" data-start=\"998\"\u003e\u003cstrong data-end=\"1008\" data-start=\"998\"\u003epFA-TF\u003c\/strong\u003e — Trigger Factor system\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-end=\"1213\" data-start=\"1036\"\u003eEach plasmid has been engineered in a stabilized, low-burden format to support consistent maintenance and reliable co-expression alongside high-level protein production systems.\u003c\/p\u003e\n\u003cp data-end=\"1398\" data-start=\"1215\"\u003eBuilt on the trusted BL21 (DE3) background, these strains enable robust T7-driven expression while minimizing proteolysis, making them ideal for producing soluble, functional protein.\u003c\/p\u003e\n\u003chr\u003e\n\u003cp\u003eFold-Assist Chemically Competent E. coli Cells are \u003cstrong\u003efree of animal-derived products\u003c\/strong\u003e and grown with animal-free media.\u003c\/p\u003e\n\u003chr\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2 data-start=\"1131\" data-end=\"1148\"\u003e\u003cstrong data-start=\"1131\" data-end=\"1148\"\u003eKey Features:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli data-end=\"1545\" data-start=\"1426\" data-section-id=\"y8iqnn\"\u003e\n\u003cp data-end=\"1545\" data-start=\"1428\"\u003e\u003cstrong data-end=\"1467\" data-start=\"1428\"\u003eFive Fold-Assist™ chaperone systems\u003c\/strong\u003e: Screen multiple folding pathways to identify optimal expression conditions\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"1695\" data-start=\"1546\" data-section-id=\"k2zwjn\"\u003e\n\u003cp data-end=\"1695\" data-start=\"1548\"\u003e\u003cstrong data-end=\"1603\" data-start=\"1548\"\u003eFunctionally aligned with industry-standard systems\u003c\/strong\u003e: Corresponding to common KJE, GroEL\/ES, and Trigger Factor-based chaperone configurations\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"1804\" data-start=\"1696\" data-section-id=\"sg8sem\"\u003e\n\u003cp data-end=\"1804\" data-start=\"1698\"\u003e\u003cstrong data-end=\"1729\" data-start=\"1698\"\u003eEnhanced protein solubility\u003c\/strong\u003e: Reduce inclusion body formation and increase recovery of active protein\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"1921\" data-start=\"1805\" data-section-id=\"1qxrqx\"\u003e\n\u003cp data-end=\"1921\" data-start=\"1807\"\u003e\u003cstrong data-end=\"1835\" data-start=\"1807\"\u003eOptimized plasmid design\u003c\/strong\u003e: Engineered for stable maintenance and reduced cellular burden during co-expression\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"2043\" data-start=\"1922\" data-section-id=\"mdlml4\"\u003e\n\u003cp data-end=\"2043\" data-start=\"1924\"\u003e\u003cstrong data-end=\"1971\" data-start=\"1924\"\u003eCompatible with standard expression vectors\u003c\/strong\u003e: Designed to perform alongside common high-expression plasmid systems\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"2152\" data-start=\"2044\" data-section-id=\"d9rtnk\"\u003e\n\u003cp data-end=\"2152\" data-start=\"2046\"\u003e\u003cstrong data-end=\"2080\" data-start=\"2046\"\u003eInducible chaperone expression\u003c\/strong\u003e: Fine-tune folding support independently of target protein production\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"2152\" data-start=\"2044\" data-section-id=\"d9rtnk\"\u003e\n\u003cstrong\u003eFree of animal derived components and grown with animal free media.\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"1916\" data-end=\"1933\"\u003e\u003cstrong data-start=\"1916\" data-end=\"1933\"\u003eApplications:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"1934\" data-end=\"2169\"\u003e\n\u003cli data-section-id=\"1jeylk\" data-start=\"1934\" data-end=\"1991\"\u003e\n\u003cp data-start=\"1936\" data-end=\"1991\"\u003eExpression of insoluble or aggregation-prone proteins\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-section-id=\"13n4aqe\" data-start=\"1992\" data-end=\"2058\"\u003e\n\u003cp data-start=\"1994\" data-end=\"2058\"\u003eProduction of eukaryotic or multi-domain proteins in \u003cem data-start=\"2047\" data-end=\"2056\"\u003eE. coli\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-section-id=\"11vc9xn\" data-start=\"2059\" data-end=\"2110\"\u003e\n\u003cp data-start=\"2061\" data-end=\"2110\"\u003eStructural biology and protein characterization\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-section-id=\"omb0iw\" data-start=\"2111\" data-end=\"2169\"\u003e\n\u003cp data-start=\"2113\" data-end=\"2169\"\u003eOptimization of recombinant protein yield and activity\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"2171\" data-end=\"2458\"\u003e\u003cstrong data-start=\"2171\" data-end=\"2194\"\u003eWorkflow Advantage:\u003c\/strong\u003e\u003cbr\u003eEach strain is pre-transformed with a chaperone plasmid, eliminating the need for sequential cloning and strain construction. This enables rapid screening of multiple folding environments using a single transformation step\u003c\/p\u003e\n\u003ch2 data-start=\"2171\" data-end=\"2458\"\u003e\u003cstrong data-start=\"1916\" data-end=\"1933\"\u003eKit Components:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e2x 100µL of each Chaperone Competent E. Coli Cell line (10x tubes in total)\u003c\/li\u003e\n\u003cli\u003e2 x 12mL Recovery Media\u003c\/li\u003e\n\u003cli\u003e1 x 10µL Control Plasmid (\u003ca href=\"https:\/\/goldbio.com\/product\/puc19-plasmid\"\u003epUC19 Control\u003c\/a\u003e, 500 pg\/µL)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cstrong data-start=\"1916\" data-end=\"1933\"\u003eStorage\/Handling:\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis product is shipped on dry ice. Chemically Competent \u003ci\u003eE. coli\u003c\/i\u003e cells should be stored at -80°C, pUC19 Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. When stored under the recommended conditions and handled correctly, these products should be stable for at least 1 year from the date of receipt.\u003c\/p\u003e","brand":"GoldBio","offers":[{"title":"10 x 100 μL","offer_id":45625009307705,"sku":"CC-330-10x100","price":149.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/files\/CC-330-Fold-AssistChemicallyCompetentE.ColiCellBundle_7bc7679f-ea25-4104-a365-8922c16e2d3f.jpg?v=1774297474"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0718\/5235\/9737\/collections\/CC-123-BL21_DE3_pLysSChemicallyCompetentE.coliCells.jpg?v=1783604018","url":"https:\/\/www.goldbio.com\/collections\/new-competent-cells.oembed","provider":"GoldBio","version":"1.0","type":"link"}