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PCR & Cloning Enzymes

T7 Endonuclease I

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Catalog ID Size Pricing
T-970-500 500 Units $ 99.00
T-970-1250 1,250 Units $ 199.00

Description

T7 Endonuclease I, a stable homodimer of identical 149 amino acid subunits is the product of a recombinant gene in E. coli. Double-stranded breaks (DSBs) generated by CRISPR/TALEN at desired target sites can be PCR-amplified, and the PCR products can be denatured and re-annealed to form mismatched DNA. If the mismatched DNA length position is more than 1 bp, T7 endonuclease I can recognize and cleave it. It is useful for quantitatively estimating the nuclease-induced mutation frequency of gene edited cells.

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T-970-500 500 Units $ 99.00
T-970-1250 1,250 Units $ 199.00

i7™ High-Fidelity DNA Polymerase

There is no image for i7™ High-Fidelity DNA Polymerase i7™ High-Fidelity DNA Polymerase
Catalog ID Size Pricing
D-920-2 200 units $ 148.00
D-920-5 500 units $ 325.00

Description

i7™ High-Fidelity DNA Polymerase is a genetically engineered, heat stable DNA polymerase which has 5´→3´ polymerase and 3´→5´ exonuclease (proofreading) activities. This enzyme has the high-fidelity, sensitivity and processivity with an error rate ~2.8×102 fold lower than Taq DNA polymerase. i7™ high-fidelity DNA polymerase is ideal for cloning and can be used for long (up to 20 kb) or difficult amplicons. This product is supplied with a proprietary 5x i7 PCR reaction buffer containing MgCl2 (final (1x) concentration of 2mM), and 5x GC enhancer that enables efficient amplification of GC rich templates up to 84%.

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D-920-2 200 units $ 148.00
D-920-5 500 units $ 325.00

T5 Exonuclease

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Catalog ID Size Pricing
T-950-1 1,000 Units $ 55.00
T-950-5 5,000 Units $ 215.00

Description

T5 Exonuclease is a highly efficient 5´→3´ exonuclease for either ssDNA or dsDNA. It also has ssDNA specific endonuclease activity in the presence of magnesium ions. However, the enzyme does not degrade supercoiled dsDNA. The mode of action of T5 Exonuclease in vivo may be analogous to that of the 5´→3´ exonuclease activity of E. coli DNA Polymerase I.

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T-950-1 1,000 Units $ 55.00
T-950-5 5,000 Units $ 215.00
RT-PCR Enzymes & Kits

Reverse Transcriptase

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Catalog ID Size Pricing
R-900-10 10,000 units $ 60.00
R-900-50 50,000 units $ 240.00

Description

GoldBio's Reverse Transcriptase (RT) is a recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase with reduced RNase H activity. It is highly efficient at producing full-length cDNA from long RNA templates. Our RT is an RNA-directed DNA polymerase which lacks 3´ → 5´ exonuclease activity and is capable of producing cDNA from as little as 50 pg of total RNA for real-time RT-PCR analysis and other applications.

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R-900-10 10,000 units $ 60.00
R-900-50 50,000 units $ 240.00

RT-PCR Kit

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Catalog ID Size Pricing
R-920-50 50 Reactions $ 160.00
R-920-200 200 Reactions $ 590.00

Description

RT-PCR Kit combines two powerful mixtures: i) 5x master mix and ii) Hot start Taq 2x master mix with standard buffer for 2-steps RT-PCR. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.

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R-920-50 50 Reactions $ 160.00
R-920-200 200 Reactions $ 590.00

One Step RT-PCR Kit

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Catalog ID Size Pricing
R-910-100 100 reactions $ 180.00
R-910-500 500 reactions $ 680.00

Description

GoldBio's One Step RT-PCR kit combines two powerful mixtures: i) Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-PCR reactions.

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R-910-100 100 reactions $ 180.00
R-910-500 500 reactions $ 680.00
qPCR & RT-qPCR Kits

qPCR Master Mix with SYBR Green®

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Catalog ID Size Pricing
M-915-100 100 reactions $ 65.00
M-915-500 500 reactions $ 229.00

Description

GoldBio's qPCR 2X Master Mix with SYBR Green® is a ready-to-use cocktail containing all components except primers and template, for the amplification and detection of DNA in qPCR. The master mix contains integrated chemically-modified hot start Taq DNA polymerase, SYBR® Green I fluorescent dye, ROX dye, MgCl2, dNTPs and stabilizers. This master mix is ideal for high-throughput real-time PCR screening and validation. The amplification step features a high quality hot start Taq DNA Polymerase which offers higher fidelity and better amplification.

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M-915-100 100 reactions $ 65.00
M-915-500 500 reactions $ 229.00

One Step RT-qPCR Kit

There is no image for One Step RT-qPCR Kit One Step RT-qPCR Kit
Catalog ID Size Pricing
R-915-100 100 reactions $ 195.00
R-915-500 500 reactions $ 695.00

Description

One step RT-qPCR kit combines two powerful mixtures: i) Reverse Transcriptase and ii) SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.

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R-915-100 100 reactions $ 195.00
R-915-500 500 reactions $ 695.00

RT-qPCR Kit

There is no image for RT-qPCR Kit RT-qPCR Kit
Catalog ID Size Pricing
R-925-50 50 reactions $ 190.00
R-925-200 200 reactions $ 640.00

Description

RT-qPCR kit combines two powerful mixtures: i) first strand cDNA synthesis kit and ii) SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range, superior sensitivity and specificity. The two mixtures require minimal handling during reaction setup and offer consistent and robust RT-qPCR reactions.

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R-925-50 50 reactions $ 190.00
R-925-200 200 reactions $ 640.00

First Strand cDNA Synthesis Kit

There is no image for First Strand cDNA Synthesis Kit First Strand cDNA Synthesis Kit
Catalog ID Size Pricing
D-925-25 25 reactions  $ 95.00
D-925-100 100 reactions $ 315.00

Description

GoldBio's First Strand cDNA synthesis kit includes our 5x Master Mix which contains: Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl2 and protein stabilizers. Reverse Transcriptase is a recombinant MMLV reverse transcriptase with reduced RNase H activity and increased thermostability. The kit also provides two optimized primers and nuclease-free water. An anchored Oligo-dT primer forces the primer to anneal to the beginning of the polyA tail and the random hexamer primer mix provides random and consistent priming sites covering the entire RNA templates including both mRNAs and non-polyadenylated RNAs. The kit is highly efficient at producing full-length cDNA from long RNA templates at temperatures between 42-55ºC.

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D-925-25 25 reactions  $ 95.00
D-925-100 100 reactions $ 315.00

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