PFGE (Pulse Field Gel Electrophoresis) Agarose is a high gel strength agarose, which allows for lower gel concentration (0.3%). PFE is designed to separate high molecular weight DNA at lower concentrations. This agarose can also be used for general nucleic acid separation and has an exceptionally low absorption of staining agents.
(I): Field Inversion Gel Electrophoresis (FIGE) separation(20-50kb): 230 V, 7.9 V/cm, 16 hrs., 50 msec. pulse, forward:reverse pulse ratio = 2.5:1. Suggested gel concentration: 0.3-1.0%.
(II): Rotating gel electrophoresis (RGE) separation (200-2000kb): 180 V, 5.1 V/cm, 34 hrs., 120 angle, 60-120 sec. pulse ramp. Suggested gel concentration 0.5-1.5%.
Gel Strength (1.5%) = >2200 g/cm2
EEO = <0.12
Gel Point = 37±1.5°C at 1.5%
Melting Point = 88±1°C at 1.5%
|Storage/Handling||Store at room temperature.|