L-Alanine 4-nitroanilide hydrochloride

H-Ala-pNAHCl is a substrate for L-alanine aminopeptidase, which is an enzyme that is localized in bacterial cell walls responsible for the cleavage of L-alanine from various peptides. Significant L-alanine aminopeptidase activity is specific to gram-negative microbes. Therefore, H-Ala-pNAHCl can be used in support of evidence that an organism is gram-positive or gram-negative. It is split by L-alanine aminopeptide into L-alanine and 4-nitroanine. The latter compound causes bacterial suspensions to turn yellow.

Product Specifications
H-Ala-pNA·HCl

MOLECULAR BIOLOGY GRADE

Formula: C₉H₁₁N₃O₃·HCl

MW: 245.66 g/mol

Storage/Handling: Store at -20°C. Protect from light.

PubChem Chemical ID: 2802426