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These substrates include chromogenic and fluorogenic substrates such as firefly luciferin, MUG, X- α-Gal, X-Gluc, as well as many others. Our superior substrates are ideal for a number of biological applications and are available for a much more affordable price so that your research dollars are focused on the things that matter." ["hide"]=> int(0) ["third_party_id"]=> int(64) ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(162) "GoldBio’s variety of fluorogenic and chromogenic substrates include firefly luciferin, MUG, X-Gluc, along with others, which are designed for many applications." 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The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI). Coelenterazine is a luminophore is the substrate for many luciferase enzymes, including Renilla reniformis, gaussia, and aequorins. It is commonly used to monitor reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS methods. Gold Biotechnology’s luciferin and coelenterazine are Proven and Published™ to ensure a quality reagent with optimal potential." 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Enzymes are important biological macromolecules that allow for the control of cellular functions and are vital for effective research. Enzyme activity can be slowed or stopped using inhibitors, like Proteinase K or RNase, while substrates like X-Gluc and X-Gal are used in detection and monitoring. D-Luciferin Potassium Salt, our most popular bioluminescent substrate, can be used for in vivo and in vitro imaging. GoldBio knows that having high quality enzyme tools are important to your laboratory, and we are committed to delivering quality products at a fraction of the price.

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object(anvilstl\commercio\models\Category)#444 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(219) ["root"]=> int(1) ["lft"]=> int(7) ["rgt"]=> int(8) ["level"]=> int(2) ["name"]=> string(10) "C_Products" ["url_name"]=> string(10) "c-products" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(0) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(219) ["root"]=> int(1) ["lft"]=> int(7) ["rgt"]=> 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object(anvilstl\commercio\models\Category)#446 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(286) ["root"]=> int(393) ["lft"]=> int(3) ["rgt"]=> int(4) ["level"]=> int(2) ["name"]=> string(15) "Luciferin (old)" ["url_name"]=> string(10) "luciferin2" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(921) "

Bioluminescence is the production and emission of light by a living organism. Bioluminescence occurs widely in marine vertebrates and invertebrates, as well as in some fungi, microorganisms and terrestrial invertebrates. Luciferin, the firefly substrate of luciferase, is a common reagent used throughout the biotechnology field. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI). Coelenterazine is a luminophore is the substrate for many luciferase enzymes, including Renilla reniformis, gaussia, and aequorins. It is commonly used to monitor reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS methods. Gold Biotechnology’s luciferin and coelenterazine are Proven and Published™ to ensure a quality reagent with optimal potential.

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object(anvilstl\commercio\models\Category)#448 (15) { ["featured_image_urls"]=> array(0) { } ["featured_image"]=> array(0) { } ["banner_image_urls"]=> array(0) { } ["productCount"]=> NULL ["_attributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> int(296) ["root"]=> int(393) ["lft"]=> int(2) ["rgt"]=> int(9) ["level"]=> int(1) ["name"]=> string(43) "Shop for Luciferins & Coelenterazines (old)" ["url_name"]=> string(30) "luciferins-and-coelenterazines" ["title"]=> NULL ["short_description"]=> string(0) "" ["long_description"]=> string(0) "" ["hide"]=> int(1) ["third_party_id"]=> NULL ["featured_image_id"]=> int(0) ["banner_image_id"]=> int(0) ["meta_description"]=> string(0) "" ["meta_keywords"]=> string(0) "" ["root_sort"]=> int(0) ["type"]=> string(7) "product" ["condition_type"]=> int(1) ["condition_match_type"]=> int(1) ["sort_type"]=> int(1) ["items_per_page"]=> int(25) ["color"]=> NULL } ["_oldAttributes":"yii\db\BaseActiveRecord":private]=> array(23) { ["id"]=> 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Coelenterazine 400 a

Name: Coelenterazine 400 a
SKU: C-320
Availability: InStock

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Product Description

Coelenterazine 400A is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.

Product Specifications:
Coelenterazine 400 a
2,8-Dibenzyl-6-phenyl-imidazo[1,2a]pyrazin-3-(7H)-one
DeepBlueC^TM

Formula: C₂₆H₂₁N₃O

MW: 391.48 g/mol

Purity: >95% by TLC

Color: Yellow to orange powder

Storage/Handling: Store at -20°C, and protect from light. Keep under inert gas.
Soluble in methanol or ethanol (NOT in DMSO).
Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.

PubChem CID: 135750016

Product Specifications

Catalog ID	C-320
CAS #	70217-82-2
MW	391.48 g/mol
Grade	MOLECULAR BIOLOGY GRADE
Storage/Handling	Store at -20°C. Protect from light.

Technical Documentation

P Protocol

O Other

C Certificate of Analysis

S Safety Data Sheet

BRET (Bioluminescence Resonance Energy Transfer) Method

High Throughput Bioluminescence Resonance Energy Transfer (BRET) Method using Rluc and Coelenterazine by SW Gersting, et al. (2012)

Coelenterazine Variants

Coelenterazine Analogue Differences

Luciferin Fact Sheet

This table includes relevant information about the luciferin substrate: source organism, species, luciferase enzyme mass, required substrate, wavelength and whether there is or is not ATP dependence.

COA - C-320

Certificate of Analysis - Coelenterazine 400a

SDS (MSDS) - Coelenterazine 400a

Safety Data Sheet