Coelenterazine 400 a

Name: Coelenterazine 400 a
SKU: C-320
Availability: InStock

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Product Description

Coelenterazine 400A is a derivative of coelenterazine and is a good substrate for Renilla reniformis luciferase (RLuc), but does not oxidize well with Gaussia (GLuc). It is the preferred substrate for BRET (Bioluminescence Resonance Energy Transfer) since its emission maximum of 400nm has minimal interference with the GFP emission.

Product Specifications:
Coelenterazine 400 a
2,8-Dibenzyl-6-phenyl-imidazo[1,2a]pyrazin-3-(7H)-one
DeepBlueC^TM

Formula: C₂₆H₂₁N₃O

MW: 391.48 g/mol

Purity: >95% by TLC

Color: Yellow to orange powder

Storage/Handling: Store at -20°C, and protect from light. Keep under inert gas.
Soluble in methanol or ethanol (NOT in DMSO).
Note: It is not recommended to store dissolved coelenterazine 400a at -20°C or -70°C. Coelenterazine 400a is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.

PubChem CID: 135750016

Product Specifications

Catalog ID	C-320
CAS #	70217-82-2
MW	391.48 g/mol
Grade	MOLECULAR BIOLOGY GRADE
Storage/Handling	Store at -20°C. Protect from light.

Technical Documentation

P Protocol

O Other

C Certificate of Analysis

S Safety Data Sheet

BRET (Bioluminescence Resonance Energy Transfer) Method

High Throughput Bioluminescence Resonance Energy Transfer (BRET) Method using Rluc and Coelenterazine by SW Gersting, et al. (2012)

Coelenterazine Variants

Coelenterazine Analogue Differences

Luciferin Fact Sheet

This table includes relevant information about the luciferin substrate: source organism, species, luciferase enzyme mass, required substrate, wavelength and whether there is or is not ATP dependence.

COA - C-320

Certificate of Analysis - Coelenterazine 400a

SDS (MSDS) - Coelenterazine 400a

Safety Data Sheet