Coelenterazine is a luminophore found in aquatic organisms and is the substrate for many luciferase enzymes, including Renilla reniformis, Gaussia and aequorins. It is commonly used for bioluminescent calcium detection and the monitoring of reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS methods as well as chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues.
MOLECULAR BIOLOGY GRADE
MW: 423.46 g/mol
Purity: >95% by TLC
Storage/Handling: Store at -20°C, and protect from light. Keep under inert gas.
Soluble in methanol or ethanol (NOT in DMSO).
Note: It is not recommended to store dissolved coelenterazine at -20°C or -70°C. Coelenterazine is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.
PubChem Chemical ID: 2830
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store under inert gas at -20°C. Protect from light.|
High Throughput Bioluminescence Resonance Energy Transfer (BRET) Method using Rluc and Coelenterazine by SW Gersting, et al. (2012)
This table includes relevant information about the luciferin substrate: source organism, species, luciferase enzyme mass, required substrate, wavelength and whether there is or is not ATP dependence.