EDTA Disodium, dihydrate
EDTA (Ethylenediaminetetraacetic acid) is a chelating agent, a general chemical and a sequestrant. In molecular biology applications, it is used to minimize metal ion contamination and prevent enzymatic activity.
EDTA disodium is routinely used in electrophoresis DNA and protein separation applications to chelate metal ions required for enzymatic activity that could potentially damage DNA and protein structure.
EDTA Disodium, Dihydrate
MOLECULAR BIOLOGY GRADE
Formula: C10H14N2Na2O8 ∙ 2H2O
MW: 372.24 g/mol
Storage/Handling: Store in a dry and well-ventilated place.
PubChem Chemical ID: 636371
|Grade||MOLECULAR BIOLOGY GRADE|
|Storage/Handling||Store desiccated at room temperature.|
High Throughput Bioluminescence Resonance Energy Transfer (BRET) Method using Rluc and Coelenterazine by SW Gersting, et al. (2012)
IPTG Induction & Extraction of Proteins - by Arur and Nayak. Schedl Lab, Wash U. St. Louis
Use of electroporation to modify Agrobacterium tumefaciens.
Use of RIPA Buffer to prepare cell lysates for protein assays, protein purification and other downstream applications.
Guide: Commonly used Buffers contains name, pH range, applications, composition and other characteristics of buffers commonly used in the lab.